Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1489

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1487 and Pf1N1B4_1488 are separated by 122 nucleotidesPf1N1B4_1488 and Pf1N1B4_1489 are separated by 12 nucleotidesPf1N1B4_1489 and Pf1N1B4_1490 are separated by 247 nucleotides Pf1N1B4_1487 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 1,713,796 to 1,715,280 _1487 Pf1N1B4_1488 - hypothetical protein, at 1,715,403 to 1,715,729 _1488 Pf1N1B4_1489 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12), at 1,715,742 to 1,719,695 _1489 Pf1N1B4_1490 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 1,719,943 to 1,721,745 _1490 Position (kb) 1715 1716 1717 1718 1719 1720Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1714.760 kb on + strand, within Pf1N1B4_1487at 1714.803 kb on - strand, within Pf1N1B4_1487at 1714.862 kb on + strand, within Pf1N1B4_1487at 1714.862 kb on + strand, within Pf1N1B4_1487at 1714.862 kb on + strand, within Pf1N1B4_1487at 1714.943 kb on + strand, within Pf1N1B4_1487at 1715.021 kb on + strand, within Pf1N1B4_1487at 1715.022 kb on - strand, within Pf1N1B4_1487at 1715.022 kb on - strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.075 kb on + strand, within Pf1N1B4_1487at 1715.076 kb on - strand, within Pf1N1B4_1487at 1715.076 kb on - strand, within Pf1N1B4_1487at 1715.076 kb on - strand, within Pf1N1B4_1487at 1715.076 kb on - strand, within Pf1N1B4_1487at 1715.076 kb on - strand, within Pf1N1B4_1487at 1715.076 kb on - strand, within Pf1N1B4_1487at 1715.076 kb on - strandat 1715.178 kb on - strandat 1715.241 kb on - strandat 1715.241 kb on - strandat 1715.241 kb on - strandat 1715.305 kb on + strandat 1715.305 kb on + strandat 1715.306 kb on - strandat 1715.306 kb on - strandat 1715.310 kb on - strandat 1715.310 kb on - strandat 1715.310 kb on - strandat 1715.375 kb on + strandat 1715.376 kb on - strandat 1715.376 kb on - strandat 1715.469 kb on + strand, within Pf1N1B4_1488at 1715.495 kb on - strand, within Pf1N1B4_1488at 1715.506 kb on - strand, within Pf1N1B4_1488at 1715.568 kb on + strand, within Pf1N1B4_1488at 1715.574 kb on + strand, within Pf1N1B4_1488at 1715.575 kb on - strand, within Pf1N1B4_1488at 1715.575 kb on - strand, within Pf1N1B4_1488at 1715.575 kb on - strand, within Pf1N1B4_1488at 1715.602 kb on + strand, within Pf1N1B4_1488at 1715.689 kb on - strand, within Pf1N1B4_1488at 1715.689 kb on - strand, within Pf1N1B4_1488at 1715.741 kb on + strandat 1715.742 kb on - strandat 1715.742 kb on - strandat 1715.742 kb on - strandat 1716.223 kb on + strand, within Pf1N1B4_1489at 1716.223 kb on + strand, within Pf1N1B4_1489at 1716.586 kb on - strand, within Pf1N1B4_1489at 1716.696 kb on + strand, within Pf1N1B4_1489at 1716.696 kb on + strand, within Pf1N1B4_1489at 1716.696 kb on + strand, within Pf1N1B4_1489at 1716.697 kb on - strand, within Pf1N1B4_1489at 1716.697 kb on - strand, within Pf1N1B4_1489at 1716.822 kb on + strand, within Pf1N1B4_1489at 1716.822 kb on + strand, within Pf1N1B4_1489at 1716.823 kb on - strandat 1716.981 kb on + strand, within Pf1N1B4_1489at 1716.981 kb on + strand, within Pf1N1B4_1489at 1717.101 kb on + strand, within Pf1N1B4_1489at 1717.101 kb on + strandat 1717.101 kb on + strand, within Pf1N1B4_1489at 1717.103 kb on + strand, within Pf1N1B4_1489at 1717.116 kb on + strand, within Pf1N1B4_1489at 1717.116 kb on + strand, within Pf1N1B4_1489at 1717.131 kb on + strand, within Pf1N1B4_1489at 1717.132 kb on - strand, within Pf1N1B4_1489at 1717.221 kb on + strand, within Pf1N1B4_1489at 1717.221 kb on + strand, within Pf1N1B4_1489at 1717.221 kb on + strand, within Pf1N1B4_1489at 1717.222 kb on - strand, within Pf1N1B4_1489at 1717.222 kb on - strand, within Pf1N1B4_1489at 1717.222 kb on - strand, within Pf1N1B4_1489at 1717.222 kb on - strand, within Pf1N1B4_1489at 1717.222 kb on - strand, within Pf1N1B4_1489at 1717.242 kb on + strandat 1717.242 kb on + strand, within Pf1N1B4_1489at 1717.243 kb on - strand, within Pf1N1B4_1489at 1717.243 kb on - strandat 1717.243 kb on - strand, within Pf1N1B4_1489at 1717.243 kb on - strand, within Pf1N1B4_1489at 1717.297 kb on - strand, within Pf1N1B4_1489at 1717.309 kb on + strand, within Pf1N1B4_1489at 1717.309 kb on + strand, within Pf1N1B4_1489at 1717.353 kb on + strand, within Pf1N1B4_1489at 1717.569 kb on + strand, within Pf1N1B4_1489at 1717.570 kb on - strand, within Pf1N1B4_1489at 1717.989 kb on + strand, within Pf1N1B4_1489at 1717.992 kb on + strand, within Pf1N1B4_1489at 1717.993 kb on - strand, within Pf1N1B4_1489at 1718.682 kb on + strand, within Pf1N1B4_1489at 1718.682 kb on + strand, within Pf1N1B4_1489at 1718.683 kb on - strand, within Pf1N1B4_1489at 1718.892 kb on - strand, within Pf1N1B4_1489at 1718.943 kb on + strand, within Pf1N1B4_1489at 1718.944 kb on - strand, within Pf1N1B4_1489at 1719.039 kb on + strand, within Pf1N1B4_1489at 1719.039 kb on + strand, within Pf1N1B4_1489at 1719.040 kb on - strandat 1719.107 kb on + strand, within Pf1N1B4_1489at 1719.214 kb on + strand, within Pf1N1B4_1489at 1719.215 kb on - strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.285 kb on + strand, within Pf1N1B4_1489at 1719.286 kb on - strand, within Pf1N1B4_1489at 1719.286 kb on - strand, within Pf1N1B4_1489at 1719.286 kb on - strand, within Pf1N1B4_1489at 1719.286 kb on - strand, within Pf1N1B4_1489at 1719.286 kb on - strand, within Pf1N1B4_1489at 1719.286 kb on - strand, within Pf1N1B4_1489at 1719.605 kb on + strandat 1719.605 kb on + strandat 1719.668 kb on + strandat 1719.669 kb on - strandat 1719.908 kb on + strandat 1719.908 kb on + strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.909 kb on - strandat 1719.934 kb on + strandat 1719.988 kb on + strandat 1719.989 kb on - strandat 1719.989 kb on - strandat 1719.989 kb on - strandat 1719.989 kb on - strandat 1720.142 kb on - strand, within Pf1N1B4_1490at 1720.257 kb on + strand, within Pf1N1B4_1490at 1720.257 kb on + strand, within Pf1N1B4_1490at 1720.257 kb on + strand, within Pf1N1B4_1490at 1720.257 kb on + strand, within Pf1N1B4_1490at 1720.258 kb on - strand, within Pf1N1B4_1490at 1720.258 kb on - strand, within Pf1N1B4_1490at 1720.258 kb on - strand, within Pf1N1B4_1490

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,714,760 + Pf1N1B4_1487 0.65 +0.2
1,714,803 - Pf1N1B4_1487 0.68 +0.4
1,714,862 + Pf1N1B4_1487 0.72 -1.3
1,714,862 + Pf1N1B4_1487 0.72 +1.1
1,714,862 + Pf1N1B4_1487 0.72 -0.0
1,714,943 + Pf1N1B4_1487 0.77 +0.4
1,715,021 + Pf1N1B4_1487 0.82 -0.2
1,715,022 - Pf1N1B4_1487 0.83 -2.2
1,715,022 - Pf1N1B4_1487 0.83 -1.2
1,715,075 + Pf1N1B4_1487 0.86 +0.4
1,715,075 + Pf1N1B4_1487 0.86 -0.8
1,715,075 + Pf1N1B4_1487 0.86 -1.9
1,715,075 + Pf1N1B4_1487 0.86 -1.6
1,715,075 + Pf1N1B4_1487 0.86 -1.6
1,715,075 + Pf1N1B4_1487 0.86 +1.4
1,715,075 + Pf1N1B4_1487 0.86 +1.4
1,715,075 + Pf1N1B4_1487 0.86 -0.2
1,715,076 - Pf1N1B4_1487 0.86 +2.0
1,715,076 - Pf1N1B4_1487 0.86 -0.6
1,715,076 - Pf1N1B4_1487 0.86 +0.4
1,715,076 - Pf1N1B4_1487 0.86 -0.2
1,715,076 - Pf1N1B4_1487 0.86 -1.2
1,715,076 - Pf1N1B4_1487 0.86 -0.7
1,715,076 - -1.2
1,715,178 - -1.0
1,715,241 - +1.4
1,715,241 - -0.4
1,715,241 - -0.6
1,715,305 + -0.6
1,715,305 + -0.6
1,715,306 - -0.6
1,715,306 - +0.4
1,715,310 - +2.0
1,715,310 - -0.2
1,715,310 - -0.3
1,715,375 + -0.9
1,715,376 - -0.5
1,715,376 - +0.4
1,715,469 + Pf1N1B4_1488 0.20 -2.6
1,715,495 - Pf1N1B4_1488 0.28 -1.1
1,715,506 - Pf1N1B4_1488 0.31 -0.3
1,715,568 + Pf1N1B4_1488 0.50 -0.6
1,715,574 + Pf1N1B4_1488 0.52 -0.6
1,715,575 - Pf1N1B4_1488 0.53 +2.0
1,715,575 - Pf1N1B4_1488 0.53 +0.2
1,715,575 - Pf1N1B4_1488 0.53 -1.6
1,715,602 + Pf1N1B4_1488 0.61 -0.6
1,715,689 - Pf1N1B4_1488 0.87 -0.0
1,715,689 - Pf1N1B4_1488 0.87 -0.8
1,715,741 + -0.0
1,715,742 - +0.7
1,715,742 - -0.6
1,715,742 - +1.0
1,716,223 + Pf1N1B4_1489 0.12 +1.4
1,716,223 + Pf1N1B4_1489 0.12 -0.0
1,716,586 - Pf1N1B4_1489 0.21 -0.6
1,716,696 + Pf1N1B4_1489 0.24 +1.1
1,716,696 + Pf1N1B4_1489 0.24 -0.3
1,716,696 + Pf1N1B4_1489 0.24 +1.2
1,716,697 - Pf1N1B4_1489 0.24 +0.7
1,716,697 - Pf1N1B4_1489 0.24 +1.0
1,716,822 + Pf1N1B4_1489 0.27 -0.6
1,716,822 + Pf1N1B4_1489 0.27 +1.2
1,716,823 - +1.7
1,716,981 + Pf1N1B4_1489 0.31 +2.0
1,716,981 + Pf1N1B4_1489 0.31 -0.3
1,717,101 + Pf1N1B4_1489 0.34 +1.8
1,717,101 + +0.4
1,717,101 + Pf1N1B4_1489 0.34 +0.4
1,717,103 + Pf1N1B4_1489 0.34 +1.0
1,717,116 + Pf1N1B4_1489 0.35 +2.7
1,717,116 + Pf1N1B4_1489 0.35 +0.0
1,717,131 + Pf1N1B4_1489 0.35 +0.9
1,717,132 - Pf1N1B4_1489 0.35 +0.4
1,717,221 + Pf1N1B4_1489 0.37 +3.2
1,717,221 + Pf1N1B4_1489 0.37 +0.8
1,717,221 + Pf1N1B4_1489 0.37 +1.7
1,717,222 - Pf1N1B4_1489 0.37 +0.7
1,717,222 - Pf1N1B4_1489 0.37 +1.0
1,717,222 - Pf1N1B4_1489 0.37 +1.4
1,717,222 - Pf1N1B4_1489 0.37 +1.4
1,717,222 - Pf1N1B4_1489 0.37 +0.8
1,717,242 + +2.4
1,717,242 + Pf1N1B4_1489 0.38 +3.0
1,717,243 - Pf1N1B4_1489 0.38 +0.1
1,717,243 - +1.4
1,717,243 - Pf1N1B4_1489 0.38 -1.2
1,717,243 - Pf1N1B4_1489 0.38 +0.4
1,717,297 - Pf1N1B4_1489 0.39 +1.4
1,717,309 + Pf1N1B4_1489 0.40 +3.7
1,717,309 + Pf1N1B4_1489 0.40 +1.3
1,717,353 + Pf1N1B4_1489 0.41 -0.9
1,717,569 + Pf1N1B4_1489 0.46 +1.7
1,717,570 - Pf1N1B4_1489 0.46 +1.0
1,717,989 + Pf1N1B4_1489 0.57 -0.8
1,717,992 + Pf1N1B4_1489 0.57 +1.1
1,717,993 - Pf1N1B4_1489 0.57 +2.2
1,718,682 + Pf1N1B4_1489 0.74 -0.3
1,718,682 + Pf1N1B4_1489 0.74 -1.2
1,718,683 - Pf1N1B4_1489 0.74 +1.2
1,718,892 - Pf1N1B4_1489 0.80 +2.6
1,718,943 + Pf1N1B4_1489 0.81 +0.8
1,718,944 - Pf1N1B4_1489 0.81 +2.2
1,719,039 + Pf1N1B4_1489 0.83 +1.4
1,719,039 + Pf1N1B4_1489 0.83 +0.8
1,719,040 - -0.6
1,719,107 + Pf1N1B4_1489 0.85 -0.8
1,719,214 + Pf1N1B4_1489 0.88 -0.9
1,719,215 - Pf1N1B4_1489 0.88 +0.4
1,719,285 + Pf1N1B4_1489 0.90 +1.6
1,719,285 + Pf1N1B4_1489 0.90 +0.4
1,719,285 + Pf1N1B4_1489 0.90 +1.4
1,719,285 + Pf1N1B4_1489 0.90 +1.4
1,719,285 + Pf1N1B4_1489 0.90 -1.2
1,719,285 + Pf1N1B4_1489 0.90 +0.7
1,719,285 + Pf1N1B4_1489 0.90 +1.8
1,719,285 + Pf1N1B4_1489 0.90 +0.8
1,719,286 - Pf1N1B4_1489 0.90 +1.8
1,719,286 - Pf1N1B4_1489 0.90 +0.8
1,719,286 - Pf1N1B4_1489 0.90 +2.7
1,719,286 - Pf1N1B4_1489 0.90 -0.3
1,719,286 - Pf1N1B4_1489 0.90 -0.3
1,719,286 - Pf1N1B4_1489 0.90 +0.9
1,719,605 + -0.4
1,719,605 + +0.4
1,719,668 + +0.4
1,719,669 - -0.4
1,719,908 + -0.0
1,719,908 + +0.4
1,719,909 - +0.4
1,719,909 - +0.1
1,719,909 - -0.6
1,719,909 - -1.6
1,719,909 - -0.6
1,719,909 - -0.2
1,719,909 - -0.8
1,719,909 - +0.7
1,719,934 + +0.8
1,719,988 + +0.3
1,719,989 - -0.1
1,719,989 - -0.6
1,719,989 - -0.6
1,719,989 - -1.9
1,720,142 - Pf1N1B4_1490 0.11 -0.4
1,720,257 + Pf1N1B4_1490 0.17 +1.4
1,720,257 + Pf1N1B4_1490 0.17 +1.0
1,720,257 + Pf1N1B4_1490 0.17 +0.4
1,720,257 + Pf1N1B4_1490 0.17 -0.4
1,720,258 - Pf1N1B4_1490 0.17 +2.0
1,720,258 - Pf1N1B4_1490 0.17 -0.8
1,720,258 - Pf1N1B4_1490 0.17 -0.6

Or see this region's nucleotide sequence