Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1487 and Pf1N1B4_1488 are separated by 122 nucleotides Pf1N1B4_1488 and Pf1N1B4_1489 are separated by 12 nucleotides Pf1N1B4_1489 and Pf1N1B4_1490 are separated by 247 nucleotides
Pf1N1B4_1487 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 1,713,796 to 1,715,280
_1487
Pf1N1B4_1488 - hypothetical protein, at 1,715,403 to 1,715,729
_1488
Pf1N1B4_1489 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12), at 1,715,742 to 1,719,695
_1489
Pf1N1B4_1490 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 1,719,943 to 1,721,745
_1490
Position (kb)
1715
1716
1717
1718
1719
1720 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1714.760 kb on + strand, within Pf1N1B4_1487 at 1714.803 kb on - strand, within Pf1N1B4_1487 at 1714.862 kb on + strand, within Pf1N1B4_1487 at 1714.862 kb on + strand, within Pf1N1B4_1487 at 1714.862 kb on + strand, within Pf1N1B4_1487 at 1714.943 kb on + strand, within Pf1N1B4_1487 at 1715.021 kb on + strand, within Pf1N1B4_1487 at 1715.022 kb on - strand, within Pf1N1B4_1487 at 1715.022 kb on - strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand at 1715.178 kb on - strand at 1715.241 kb on - strand at 1715.241 kb on - strand at 1715.241 kb on - strand at 1715.305 kb on + strand at 1715.305 kb on + strand at 1715.306 kb on - strand at 1715.306 kb on - strand at 1715.310 kb on - strand at 1715.310 kb on - strand at 1715.310 kb on - strand at 1715.375 kb on + strand at 1715.376 kb on - strand at 1715.376 kb on - strand at 1715.469 kb on + strand, within Pf1N1B4_1488 at 1715.495 kb on - strand, within Pf1N1B4_1488 at 1715.506 kb on - strand, within Pf1N1B4_1488 at 1715.568 kb on + strand, within Pf1N1B4_1488 at 1715.574 kb on + strand, within Pf1N1B4_1488 at 1715.575 kb on - strand, within Pf1N1B4_1488 at 1715.575 kb on - strand, within Pf1N1B4_1488 at 1715.575 kb on - strand, within Pf1N1B4_1488 at 1715.602 kb on + strand, within Pf1N1B4_1488 at 1715.689 kb on - strand, within Pf1N1B4_1488 at 1715.689 kb on - strand, within Pf1N1B4_1488 at 1715.741 kb on + strand at 1715.742 kb on - strand at 1715.742 kb on - strand at 1715.742 kb on - strand at 1716.223 kb on + strand, within Pf1N1B4_1489 at 1716.223 kb on + strand, within Pf1N1B4_1489 at 1716.586 kb on - strand, within Pf1N1B4_1489 at 1716.696 kb on + strand, within Pf1N1B4_1489 at 1716.696 kb on + strand, within Pf1N1B4_1489 at 1716.696 kb on + strand, within Pf1N1B4_1489 at 1716.697 kb on - strand, within Pf1N1B4_1489 at 1716.697 kb on - strand, within Pf1N1B4_1489 at 1716.822 kb on + strand, within Pf1N1B4_1489 at 1716.822 kb on + strand, within Pf1N1B4_1489 at 1716.823 kb on - strand at 1716.981 kb on + strand, within Pf1N1B4_1489 at 1716.981 kb on + strand, within Pf1N1B4_1489 at 1717.101 kb on + strand, within Pf1N1B4_1489 at 1717.101 kb on + strand at 1717.101 kb on + strand, within Pf1N1B4_1489 at 1717.103 kb on + strand, within Pf1N1B4_1489 at 1717.116 kb on + strand, within Pf1N1B4_1489 at 1717.116 kb on + strand, within Pf1N1B4_1489 at 1717.131 kb on + strand, within Pf1N1B4_1489 at 1717.132 kb on - strand, within Pf1N1B4_1489 at 1717.221 kb on + strand, within Pf1N1B4_1489 at 1717.221 kb on + strand, within Pf1N1B4_1489 at 1717.221 kb on + strand, within Pf1N1B4_1489 at 1717.222 kb on - strand, within Pf1N1B4_1489 at 1717.222 kb on - strand, within Pf1N1B4_1489 at 1717.222 kb on - strand, within Pf1N1B4_1489 at 1717.222 kb on - strand, within Pf1N1B4_1489 at 1717.222 kb on - strand, within Pf1N1B4_1489 at 1717.242 kb on + strand at 1717.242 kb on + strand, within Pf1N1B4_1489 at 1717.243 kb on - strand, within Pf1N1B4_1489 at 1717.243 kb on - strand at 1717.243 kb on - strand, within Pf1N1B4_1489 at 1717.243 kb on - strand, within Pf1N1B4_1489 at 1717.297 kb on - strand, within Pf1N1B4_1489 at 1717.309 kb on + strand, within Pf1N1B4_1489 at 1717.309 kb on + strand, within Pf1N1B4_1489 at 1717.353 kb on + strand, within Pf1N1B4_1489 at 1717.569 kb on + strand, within Pf1N1B4_1489 at 1717.570 kb on - strand, within Pf1N1B4_1489 at 1717.989 kb on + strand, within Pf1N1B4_1489 at 1717.992 kb on + strand, within Pf1N1B4_1489 at 1717.993 kb on - strand, within Pf1N1B4_1489 at 1718.682 kb on + strand, within Pf1N1B4_1489 at 1718.682 kb on + strand, within Pf1N1B4_1489 at 1718.683 kb on - strand, within Pf1N1B4_1489 at 1718.892 kb on - strand, within Pf1N1B4_1489 at 1718.943 kb on + strand, within Pf1N1B4_1489 at 1718.944 kb on - strand, within Pf1N1B4_1489 at 1719.039 kb on + strand, within Pf1N1B4_1489 at 1719.039 kb on + strand, within Pf1N1B4_1489 at 1719.040 kb on - strand at 1719.107 kb on + strand, within Pf1N1B4_1489 at 1719.214 kb on + strand, within Pf1N1B4_1489 at 1719.215 kb on - strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.285 kb on + strand, within Pf1N1B4_1489 at 1719.286 kb on - strand, within Pf1N1B4_1489 at 1719.286 kb on - strand, within Pf1N1B4_1489 at 1719.286 kb on - strand, within Pf1N1B4_1489 at 1719.286 kb on - strand, within Pf1N1B4_1489 at 1719.286 kb on - strand, within Pf1N1B4_1489 at 1719.286 kb on - strand, within Pf1N1B4_1489 at 1719.605 kb on + strand at 1719.605 kb on + strand at 1719.668 kb on + strand at 1719.669 kb on - strand at 1719.908 kb on + strand at 1719.908 kb on + strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.909 kb on - strand at 1719.934 kb on + strand at 1719.988 kb on + strand at 1719.989 kb on - strand at 1719.989 kb on - strand at 1719.989 kb on - strand at 1719.989 kb on - strand at 1720.142 kb on - strand, within Pf1N1B4_1490 at 1720.257 kb on + strand, within Pf1N1B4_1490 at 1720.257 kb on + strand, within Pf1N1B4_1490 at 1720.257 kb on + strand, within Pf1N1B4_1490 at 1720.257 kb on + strand, within Pf1N1B4_1490 at 1720.258 kb on - strand, within Pf1N1B4_1490 at 1720.258 kb on - strand, within Pf1N1B4_1490 at 1720.258 kb on - strand, within Pf1N1B4_1490
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,714,760 + Pf1N1B4_1487 0.65 +0.2 1,714,803 - Pf1N1B4_1487 0.68 +0.4 1,714,862 + Pf1N1B4_1487 0.72 -1.3 1,714,862 + Pf1N1B4_1487 0.72 +1.1 1,714,862 + Pf1N1B4_1487 0.72 -0.0 1,714,943 + Pf1N1B4_1487 0.77 +0.4 1,715,021 + Pf1N1B4_1487 0.82 -0.2 1,715,022 - Pf1N1B4_1487 0.83 -2.2 1,715,022 - Pf1N1B4_1487 0.83 -1.2 1,715,075 + Pf1N1B4_1487 0.86 +0.4 1,715,075 + Pf1N1B4_1487 0.86 -0.8 1,715,075 + Pf1N1B4_1487 0.86 -1.9 1,715,075 + Pf1N1B4_1487 0.86 -1.6 1,715,075 + Pf1N1B4_1487 0.86 -1.6 1,715,075 + Pf1N1B4_1487 0.86 +1.4 1,715,075 + Pf1N1B4_1487 0.86 +1.4 1,715,075 + Pf1N1B4_1487 0.86 -0.2 1,715,076 - Pf1N1B4_1487 0.86 +2.0 1,715,076 - Pf1N1B4_1487 0.86 -0.6 1,715,076 - Pf1N1B4_1487 0.86 +0.4 1,715,076 - Pf1N1B4_1487 0.86 -0.2 1,715,076 - Pf1N1B4_1487 0.86 -1.2 1,715,076 - Pf1N1B4_1487 0.86 -0.7 1,715,076 - -1.2 1,715,178 - -1.0 1,715,241 - +1.4 1,715,241 - -0.4 1,715,241 - -0.6 1,715,305 + -0.6 1,715,305 + -0.6 1,715,306 - -0.6 1,715,306 - +0.4 1,715,310 - +2.0 1,715,310 - -0.2 1,715,310 - -0.3 1,715,375 + -0.9 1,715,376 - -0.5 1,715,376 - +0.4 1,715,469 + Pf1N1B4_1488 0.20 -2.6 1,715,495 - Pf1N1B4_1488 0.28 -1.1 1,715,506 - Pf1N1B4_1488 0.31 -0.3 1,715,568 + Pf1N1B4_1488 0.50 -0.6 1,715,574 + Pf1N1B4_1488 0.52 -0.6 1,715,575 - Pf1N1B4_1488 0.53 +2.0 1,715,575 - Pf1N1B4_1488 0.53 +0.2 1,715,575 - Pf1N1B4_1488 0.53 -1.6 1,715,602 + Pf1N1B4_1488 0.61 -0.6 1,715,689 - Pf1N1B4_1488 0.87 -0.0 1,715,689 - Pf1N1B4_1488 0.87 -0.8 1,715,741 + -0.0 1,715,742 - +0.7 1,715,742 - -0.6 1,715,742 - +1.0 1,716,223 + Pf1N1B4_1489 0.12 +1.4 1,716,223 + Pf1N1B4_1489 0.12 -0.0 1,716,586 - Pf1N1B4_1489 0.21 -0.6 1,716,696 + Pf1N1B4_1489 0.24 +1.1 1,716,696 + Pf1N1B4_1489 0.24 -0.3 1,716,696 + Pf1N1B4_1489 0.24 +1.2 1,716,697 - Pf1N1B4_1489 0.24 +0.7 1,716,697 - Pf1N1B4_1489 0.24 +1.0 1,716,822 + Pf1N1B4_1489 0.27 -0.6 1,716,822 + Pf1N1B4_1489 0.27 +1.2 1,716,823 - +1.7 1,716,981 + Pf1N1B4_1489 0.31 +2.0 1,716,981 + Pf1N1B4_1489 0.31 -0.3 1,717,101 + Pf1N1B4_1489 0.34 +1.8 1,717,101 + +0.4 1,717,101 + Pf1N1B4_1489 0.34 +0.4 1,717,103 + Pf1N1B4_1489 0.34 +1.0 1,717,116 + Pf1N1B4_1489 0.35 +2.7 1,717,116 + Pf1N1B4_1489 0.35 +0.0 1,717,131 + Pf1N1B4_1489 0.35 +0.9 1,717,132 - Pf1N1B4_1489 0.35 +0.4 1,717,221 + Pf1N1B4_1489 0.37 +3.2 1,717,221 + Pf1N1B4_1489 0.37 +0.8 1,717,221 + Pf1N1B4_1489 0.37 +1.7 1,717,222 - Pf1N1B4_1489 0.37 +0.7 1,717,222 - Pf1N1B4_1489 0.37 +1.0 1,717,222 - Pf1N1B4_1489 0.37 +1.4 1,717,222 - Pf1N1B4_1489 0.37 +1.4 1,717,222 - Pf1N1B4_1489 0.37 +0.8 1,717,242 + +2.4 1,717,242 + Pf1N1B4_1489 0.38 +3.0 1,717,243 - Pf1N1B4_1489 0.38 +0.1 1,717,243 - +1.4 1,717,243 - Pf1N1B4_1489 0.38 -1.2 1,717,243 - Pf1N1B4_1489 0.38 +0.4 1,717,297 - Pf1N1B4_1489 0.39 +1.4 1,717,309 + Pf1N1B4_1489 0.40 +3.7 1,717,309 + Pf1N1B4_1489 0.40 +1.3 1,717,353 + Pf1N1B4_1489 0.41 -0.9 1,717,569 + Pf1N1B4_1489 0.46 +1.7 1,717,570 - Pf1N1B4_1489 0.46 +1.0 1,717,989 + Pf1N1B4_1489 0.57 -0.8 1,717,992 + Pf1N1B4_1489 0.57 +1.1 1,717,993 - Pf1N1B4_1489 0.57 +2.2 1,718,682 + Pf1N1B4_1489 0.74 -0.3 1,718,682 + Pf1N1B4_1489 0.74 -1.2 1,718,683 - Pf1N1B4_1489 0.74 +1.2 1,718,892 - Pf1N1B4_1489 0.80 +2.6 1,718,943 + Pf1N1B4_1489 0.81 +0.8 1,718,944 - Pf1N1B4_1489 0.81 +2.2 1,719,039 + Pf1N1B4_1489 0.83 +1.4 1,719,039 + Pf1N1B4_1489 0.83 +0.8 1,719,040 - -0.6 1,719,107 + Pf1N1B4_1489 0.85 -0.8 1,719,214 + Pf1N1B4_1489 0.88 -0.9 1,719,215 - Pf1N1B4_1489 0.88 +0.4 1,719,285 + Pf1N1B4_1489 0.90 +1.6 1,719,285 + Pf1N1B4_1489 0.90 +0.4 1,719,285 + Pf1N1B4_1489 0.90 +1.4 1,719,285 + Pf1N1B4_1489 0.90 +1.4 1,719,285 + Pf1N1B4_1489 0.90 -1.2 1,719,285 + Pf1N1B4_1489 0.90 +0.7 1,719,285 + Pf1N1B4_1489 0.90 +1.8 1,719,285 + Pf1N1B4_1489 0.90 +0.8 1,719,286 - Pf1N1B4_1489 0.90 +1.8 1,719,286 - Pf1N1B4_1489 0.90 +0.8 1,719,286 - Pf1N1B4_1489 0.90 +2.7 1,719,286 - Pf1N1B4_1489 0.90 -0.3 1,719,286 - Pf1N1B4_1489 0.90 -0.3 1,719,286 - Pf1N1B4_1489 0.90 +0.9 1,719,605 + -0.4 1,719,605 + +0.4 1,719,668 + +0.4 1,719,669 - -0.4 1,719,908 + -0.0 1,719,908 + +0.4 1,719,909 - +0.4 1,719,909 - +0.1 1,719,909 - -0.6 1,719,909 - -1.6 1,719,909 - -0.6 1,719,909 - -0.2 1,719,909 - -0.8 1,719,909 - +0.7 1,719,934 + +0.8 1,719,988 + +0.3 1,719,989 - -0.1 1,719,989 - -0.6 1,719,989 - -0.6 1,719,989 - -1.9 1,720,142 - Pf1N1B4_1490 0.11 -0.4 1,720,257 + Pf1N1B4_1490 0.17 +1.4 1,720,257 + Pf1N1B4_1490 0.17 +1.0 1,720,257 + Pf1N1B4_1490 0.17 +0.4 1,720,257 + Pf1N1B4_1490 0.17 -0.4 1,720,258 - Pf1N1B4_1490 0.17 +2.0 1,720,258 - Pf1N1B4_1490 0.17 -0.8 1,720,258 - Pf1N1B4_1490 0.17 -0.6
Or see this region's nucleotide sequence