Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1402 and Pf1N1B4_1403 overlap by 4 nucleotides Pf1N1B4_1403 and Pf1N1B4_5183 are separated by 226 nucleotides Pf1N1B4_5183 and Pf1N1B4_1404 are separated by 337 nucleotides
Pf1N1B4_1402 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 1,605,577 to 1,606,338
_1402
Pf1N1B4_1403 - 3'-to-5' exoribonuclease RNase R, at 1,606,335 to 1,608,965
_1403
Pf1N1B4_5183 - tRNA-Leu-CAG, at 1,609,192 to 1,609,275
_5183
Pf1N1B4_1404 - Ferric iron ABC transporter, iron-binding protein, at 1,609,613 to 1,610,623
_1404
Position (kb)
1606
1607
1608
1609 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1605.433 kb on + strand at 1605.434 kb on - strand at 1605.434 kb on - strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.535 kb on + strand at 1605.535 kb on + strand at 1605.536 kb on - strand at 1605.536 kb on - strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.699 kb on - strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.707 kb on - strand, within Pf1N1B4_1402 at 1605.707 kb on - strand, within Pf1N1B4_1402 at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.349 kb on + strand at 1606.350 kb on - strand at 1606.544 kb on + strand at 1606.837 kb on - strand, within Pf1N1B4_1403 at 1606.855 kb on + strand, within Pf1N1B4_1403 at 1606.856 kb on - strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1607.230 kb on + strand, within Pf1N1B4_1403 at 1607.231 kb on - strand, within Pf1N1B4_1403 at 1607.239 kb on + strand at 1607.240 kb on - strand, within Pf1N1B4_1403 at 1607.275 kb on + strand, within Pf1N1B4_1403 at 1607.276 kb on - strand, within Pf1N1B4_1403 at 1607.276 kb on - strand, within Pf1N1B4_1403 at 1607.410 kb on - strand, within Pf1N1B4_1403 at 1607.410 kb on - strand, within Pf1N1B4_1403 at 1607.680 kb on + strand, within Pf1N1B4_1403 at 1607.723 kb on - strand, within Pf1N1B4_1403 at 1607.787 kb on - strand, within Pf1N1B4_1403 at 1607.788 kb on + strand, within Pf1N1B4_1403 at 1607.788 kb on + strand, within Pf1N1B4_1403 at 1607.789 kb on - strand, within Pf1N1B4_1403 at 1607.789 kb on - strand, within Pf1N1B4_1403 at 1607.824 kb on + strand, within Pf1N1B4_1403 at 1607.966 kb on - strand, within Pf1N1B4_1403 at 1607.966 kb on - strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.050 kb on - strand, within Pf1N1B4_1403 at 1608.050 kb on - strand, within Pf1N1B4_1403 at 1608.050 kb on - strand, within Pf1N1B4_1403 at 1608.262 kb on + strand, within Pf1N1B4_1403 at 1608.263 kb on - strand at 1608.263 kb on - strand, within Pf1N1B4_1403 at 1608.263 kb on - strand, within Pf1N1B4_1403 at 1608.263 kb on - strand at 1608.304 kb on + strand, within Pf1N1B4_1403 at 1608.304 kb on + strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.487 kb on + strand, within Pf1N1B4_1403 at 1608.727 kb on + strand at 1608.727 kb on + strand at 1608.727 kb on + strand at 1608.728 kb on - strand at 1608.728 kb on - strand at 1608.745 kb on + strand at 1608.745 kb on + strand at 1608.745 kb on + strand at 1608.746 kb on - strand at 1608.986 kb on + strand at 1608.997 kb on + strand at 1609.050 kb on + strand at 1609.367 kb on + strand at 1609.367 kb on + strand at 1609.367 kb on + strand at 1609.368 kb on - strand at 1609.434 kb on - strand at 1609.434 kb on - strand at 1609.434 kb on - strand at 1609.516 kb on + strand at 1609.517 kb on - strand at 1609.575 kb on + strand at 1609.627 kb on + strand at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.868 kb on + strand, within Pf1N1B4_1404 at 1609.868 kb on + strand, within Pf1N1B4_1404 at 1609.868 kb on + strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,605,433 + -2.7 1,605,434 - -0.7 1,605,434 - +1.3 1,605,463 + +0.7 1,605,463 + +2.7 1,605,463 + +0.3 1,605,463 + -0.1 1,605,463 + +0.3 1,605,464 - +2.7 1,605,464 - +0.3 1,605,464 - +1.3 1,605,464 - +0.8 1,605,464 - +0.1 1,605,535 + +0.3 1,605,535 + -0.7 1,605,536 - -0.9 1,605,536 - +0.3 1,605,562 + -0.7 1,605,562 + -0.1 1,605,562 + -0.4 1,605,562 + -0.2 1,605,562 + +0.2 1,605,562 + +0.7 1,605,563 - +0.3 1,605,563 - +0.9 1,605,563 - +1.3 1,605,563 - -0.1 1,605,563 - -0.6 1,605,563 - +0.3 1,605,699 - Pf1N1B4_1402 0.16 -0.9 1,605,706 + Pf1N1B4_1402 0.17 -0.4 1,605,706 + Pf1N1B4_1402 0.17 -1.1 1,605,706 + Pf1N1B4_1402 0.17 -0.0 1,605,706 + Pf1N1B4_1402 0.17 -1.7 1,605,707 - Pf1N1B4_1402 0.17 -1.7 1,605,707 - Pf1N1B4_1402 0.17 -0.6 1,606,316 + +0.9 1,606,316 + +0.5 1,606,316 + -0.8 1,606,316 + +1.9 1,606,317 - -1.7 1,606,317 - -0.1 1,606,317 - -0.1 1,606,317 - +0.3 1,606,349 + +0.6 1,606,350 - -0.1 1,606,544 + -1.0 1,606,837 - Pf1N1B4_1403 0.19 +1.5 1,606,855 + Pf1N1B4_1403 0.20 -0.1 1,606,856 - Pf1N1B4_1403 0.20 -2.0 1,606,897 + Pf1N1B4_1403 0.21 +1.3 1,606,897 + Pf1N1B4_1403 0.21 +0.3 1,606,897 + Pf1N1B4_1403 0.21 -0.1 1,606,897 + Pf1N1B4_1403 0.21 -0.9 1,606,898 - Pf1N1B4_1403 0.21 -0.5 1,606,898 - Pf1N1B4_1403 0.21 +0.3 1,606,898 - Pf1N1B4_1403 0.21 +0.6 1,607,230 + Pf1N1B4_1403 0.34 -0.7 1,607,231 - Pf1N1B4_1403 0.34 +1.9 1,607,239 + +1.3 1,607,240 - Pf1N1B4_1403 0.34 +2.7 1,607,275 + Pf1N1B4_1403 0.36 +1.9 1,607,276 - Pf1N1B4_1403 0.36 +0.3 1,607,276 - Pf1N1B4_1403 0.36 +1.7 1,607,410 - Pf1N1B4_1403 0.41 +2.7 1,607,410 - Pf1N1B4_1403 0.41 +0.3 1,607,680 + Pf1N1B4_1403 0.51 -0.4 1,607,723 - Pf1N1B4_1403 0.53 -0.1 1,607,787 - Pf1N1B4_1403 0.55 -2.5 1,607,788 + Pf1N1B4_1403 0.55 +1.9 1,607,788 + Pf1N1B4_1403 0.55 -0.1 1,607,789 - Pf1N1B4_1403 0.55 +0.3 1,607,789 - Pf1N1B4_1403 0.55 +1.2 1,607,824 + Pf1N1B4_1403 0.57 -0.5 1,607,966 - Pf1N1B4_1403 0.62 +3.2 1,607,966 - Pf1N1B4_1403 0.62 +1.7 1,608,049 + Pf1N1B4_1403 0.65 +1.9 1,608,049 + Pf1N1B4_1403 0.65 -1.0 1,608,049 + Pf1N1B4_1403 0.65 -1.7 1,608,049 + Pf1N1B4_1403 0.65 +0.1 1,608,050 - Pf1N1B4_1403 0.65 -1.2 1,608,050 - Pf1N1B4_1403 0.65 -1.5 1,608,050 - Pf1N1B4_1403 0.65 +1.9 1,608,262 + Pf1N1B4_1403 0.73 +2.7 1,608,263 - -0.4 1,608,263 - Pf1N1B4_1403 0.73 +1.3 1,608,263 - Pf1N1B4_1403 0.73 +2.3 1,608,263 - +0.9 1,608,304 + Pf1N1B4_1403 0.75 +0.8 1,608,304 + Pf1N1B4_1403 0.75 -0.7 1,608,305 - Pf1N1B4_1403 0.75 +1.5 1,608,305 - Pf1N1B4_1403 0.75 +2.3 1,608,305 - Pf1N1B4_1403 0.75 -1.7 1,608,305 - Pf1N1B4_1403 0.75 -0.2 1,608,487 + Pf1N1B4_1403 0.82 +0.8 1,608,727 + -1.7 1,608,727 + +0.9 1,608,727 + +0.3 1,608,728 - -2.0 1,608,728 - -0.7 1,608,745 + +0.3 1,608,745 + +0.1 1,608,745 + +0.9 1,608,746 - +0.3 1,608,986 + +0.3 1,608,997 + +1.3 1,609,050 + -0.3 1,609,367 + -0.7 1,609,367 + +0.3 1,609,367 + -2.1 1,609,368 - -0.9 1,609,434 - -0.4 1,609,434 - -0.7 1,609,434 - -0.2 1,609,516 + +0.3 1,609,517 - -1.0 1,609,575 + +1.3 1,609,627 + -1.2 1,609,718 + Pf1N1B4_1404 0.10 +1.3 1,609,718 + Pf1N1B4_1404 0.10 -0.2 1,609,718 + Pf1N1B4_1404 0.10 +1.9 1,609,718 + Pf1N1B4_1404 0.10 +1.3 1,609,719 - Pf1N1B4_1404 0.10 -0.1 1,609,719 - Pf1N1B4_1404 0.10 -0.1 1,609,719 - Pf1N1B4_1404 0.10 -0.2 1,609,719 - Pf1N1B4_1404 0.10 -0.7 1,609,719 - Pf1N1B4_1404 0.10 -0.7 1,609,868 + Pf1N1B4_1404 0.25 +0.3 1,609,868 + Pf1N1B4_1404 0.25 +0.3 1,609,868 + Pf1N1B4_1404 0.25 -1.1 1,609,869 - Pf1N1B4_1404 0.25 -0.7 1,609,869 - +0.3 1,609,869 - Pf1N1B4_1404 0.25 +0.1 1,609,869 - Pf1N1B4_1404 0.25 +0.9 1,609,869 - Pf1N1B4_1404 0.25 +1.3 1,609,869 - Pf1N1B4_1404 0.25 -0.2 1,609,961 + Pf1N1B4_1404 0.34 -0.4 1,609,961 + Pf1N1B4_1404 0.34 -1.2 1,609,961 + Pf1N1B4_1404 0.34 -0.3 1,609,961 + Pf1N1B4_1404 0.34 +1.1 1,609,962 - Pf1N1B4_1404 0.35 -0.6 1,609,962 - Pf1N1B4_1404 0.35 -1.2 1,609,962 - Pf1N1B4_1404 0.35 +1.2 1,609,962 - Pf1N1B4_1404 0.35 -1.2
Or see this region's nucleotide sequence