Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1377 and Pf1N1B4_1378 are separated by 211 nucleotides Pf1N1B4_1378 and Pf1N1B4_1379 overlap by 4 nucleotides Pf1N1B4_1379 and Pf1N1B4_1380 overlap by 4 nucleotides
Pf1N1B4_1377 - Mobile element protein, at 1,575,567 to 1,576,709
_1377
Pf1N1B4_1378 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 1,576,921 to 1,577,637
_1378
Pf1N1B4_1379 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,577,634 to 1,578,506
_1379
Pf1N1B4_1380 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,578,503 to 1,579,786
_1380
Position (kb)
1577
1578
1579 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1576.863 kb on + strand at 1576.863 kb on + strand at 1576.863 kb on + strand at 1576.863 kb on + strand at 1576.864 kb on - strand at 1576.938 kb on - strand at 1577.015 kb on + strand, within Pf1N1B4_1378 at 1577.016 kb on - strand, within Pf1N1B4_1378 at 1577.016 kb on - strand, within Pf1N1B4_1378 at 1577.016 kb on - strand, within Pf1N1B4_1378 at 1577.076 kb on + strand, within Pf1N1B4_1378 at 1577.076 kb on + strand, within Pf1N1B4_1378 at 1577.077 kb on - strand, within Pf1N1B4_1378 at 1577.303 kb on + strand, within Pf1N1B4_1378 at 1577.304 kb on - strand, within Pf1N1B4_1378 at 1577.304 kb on - strand, within Pf1N1B4_1378 at 1577.411 kb on + strand, within Pf1N1B4_1378 at 1577.411 kb on + strand, within Pf1N1B4_1378 at 1577.411 kb on + strand, within Pf1N1B4_1378 at 1577.411 kb on + strand, within Pf1N1B4_1378 at 1577.412 kb on - strand, within Pf1N1B4_1378 at 1577.412 kb on - strand, within Pf1N1B4_1378 at 1577.412 kb on - strand, within Pf1N1B4_1378 at 1577.447 kb on + strand, within Pf1N1B4_1378 at 1577.448 kb on - strand, within Pf1N1B4_1378 at 1577.595 kb on - strand at 1577.631 kb on + strand at 1577.632 kb on - strand at 1577.637 kb on + strand at 1577.637 kb on + strand at 1577.638 kb on - strand at 1577.638 kb on - strand at 1577.638 kb on - strand at 1577.638 kb on - strand at 1577.740 kb on - strand, within Pf1N1B4_1379 at 1577.740 kb on - strand, within Pf1N1B4_1379 at 1577.740 kb on - strand, within Pf1N1B4_1379 at 1577.760 kb on - strand, within Pf1N1B4_1379 at 1577.760 kb on - strand, within Pf1N1B4_1379 at 1577.760 kb on - strand, within Pf1N1B4_1379 at 1577.766 kb on + strand, within Pf1N1B4_1379 at 1577.950 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.098 kb on + strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.099 kb on - strand, within Pf1N1B4_1379 at 1578.215 kb on - strand, within Pf1N1B4_1379 at 1578.230 kb on + strand, within Pf1N1B4_1379 at 1578.343 kb on + strand, within Pf1N1B4_1379 at 1578.344 kb on - strand, within Pf1N1B4_1379 at 1578.344 kb on - strand, within Pf1N1B4_1379 at 1578.344 kb on - strand, within Pf1N1B4_1379 at 1578.492 kb on + strand at 1578.493 kb on - strand at 1578.512 kb on + strand at 1578.512 kb on + strand at 1578.513 kb on - strand at 1578.520 kb on + strand at 1578.584 kb on + strand at 1578.584 kb on + strand at 1578.585 kb on - strand at 1578.585 kb on - strand at 1578.585 kb on - strand at 1578.771 kb on + strand, within Pf1N1B4_1380 at 1578.771 kb on + strand, within Pf1N1B4_1380 at 1578.772 kb on - strand, within Pf1N1B4_1380 at 1578.772 kb on - strand, within Pf1N1B4_1380 at 1578.772 kb on - strand, within Pf1N1B4_1380 at 1578.772 kb on - strand, within Pf1N1B4_1380 at 1578.772 kb on - strand, within Pf1N1B4_1380 at 1578.772 kb on - strand, within Pf1N1B4_1380 at 1578.776 kb on - strand, within Pf1N1B4_1380 at 1578.776 kb on - strand, within Pf1N1B4_1380 at 1578.776 kb on - strand, within Pf1N1B4_1380 at 1578.776 kb on - strand, within Pf1N1B4_1380 at 1578.842 kb on + strand, within Pf1N1B4_1380 at 1578.842 kb on + strand, within Pf1N1B4_1380 at 1578.842 kb on + strand, within Pf1N1B4_1380 at 1578.842 kb on + strand, within Pf1N1B4_1380 at 1578.842 kb on + strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.843 kb on - strand, within Pf1N1B4_1380 at 1578.939 kb on + strand, within Pf1N1B4_1380 at 1578.939 kb on + strand, within Pf1N1B4_1380 at 1578.939 kb on + strand, within Pf1N1B4_1380 at 1578.939 kb on + strand, within Pf1N1B4_1380 at 1578.975 kb on + strand, within Pf1N1B4_1380 at 1578.975 kb on + strand, within Pf1N1B4_1380 at 1578.975 kb on + strand, within Pf1N1B4_1380 at 1578.975 kb on + strand, within Pf1N1B4_1380 at 1578.975 kb on + strand, within Pf1N1B4_1380 at 1578.975 kb on + strand, within Pf1N1B4_1380 at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.976 kb on - strand at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.976 kb on - strand, within Pf1N1B4_1380 at 1578.977 kb on + strand, within Pf1N1B4_1380 at 1578.977 kb on + strand, within Pf1N1B4_1380 at 1578.977 kb on + strand at 1578.977 kb on + strand, within Pf1N1B4_1380 at 1578.978 kb on - strand, within Pf1N1B4_1380 at 1578.978 kb on - strand, within Pf1N1B4_1380 at 1578.978 kb on - strand, within Pf1N1B4_1380 at 1578.978 kb on - strand, within Pf1N1B4_1380 at 1578.984 kb on + strand at 1578.984 kb on + strand, within Pf1N1B4_1380 at 1578.985 kb on - strand, within Pf1N1B4_1380 at 1578.985 kb on - strand, within Pf1N1B4_1380 at 1578.985 kb on - strand, within Pf1N1B4_1380 at 1578.985 kb on - strand, within Pf1N1B4_1380 at 1578.985 kb on - strand, within Pf1N1B4_1380 at 1578.985 kb on - strand, within Pf1N1B4_1380 at 1578.988 kb on - strand, within Pf1N1B4_1380 at 1578.988 kb on - strand at 1578.988 kb on - strand, within Pf1N1B4_1380 at 1578.988 kb on - strand, within Pf1N1B4_1380 at 1578.988 kb on - strand, within Pf1N1B4_1380 at 1578.988 kb on - strand, within Pf1N1B4_1380 at 1579.005 kb on + strand, within Pf1N1B4_1380 at 1579.005 kb on + strand, within Pf1N1B4_1380 at 1579.006 kb on - strand, within Pf1N1B4_1380 at 1579.006 kb on - strand, within Pf1N1B4_1380 at 1579.006 kb on - strand, within Pf1N1B4_1380 at 1579.006 kb on - strand, within Pf1N1B4_1380 at 1579.099 kb on + strand, within Pf1N1B4_1380 at 1579.133 kb on - strand, within Pf1N1B4_1380 at 1579.133 kb on - strand, within Pf1N1B4_1380 at 1579.185 kb on + strand, within Pf1N1B4_1380 at 1579.186 kb on - strand, within Pf1N1B4_1380 at 1579.205 kb on + strand, within Pf1N1B4_1380 at 1579.206 kb on - strand, within Pf1N1B4_1380 at 1579.233 kb on - strand, within Pf1N1B4_1380 at 1579.287 kb on + strand, within Pf1N1B4_1380 at 1579.288 kb on - strand, within Pf1N1B4_1380 at 1579.288 kb on - strand, within Pf1N1B4_1380 at 1579.326 kb on + strand, within Pf1N1B4_1380 at 1579.327 kb on - strand, within Pf1N1B4_1380 at 1579.338 kb on + strand, within Pf1N1B4_1380 at 1579.401 kb on + strand, within Pf1N1B4_1380 at 1579.401 kb on + strand, within Pf1N1B4_1380 at 1579.401 kb on + strand, within Pf1N1B4_1380 at 1579.401 kb on + strand, within Pf1N1B4_1380 at 1579.401 kb on + strand, within Pf1N1B4_1380 at 1579.401 kb on + strand, within Pf1N1B4_1380 at 1579.402 kb on - strand, within Pf1N1B4_1380 at 1579.402 kb on - strand, within Pf1N1B4_1380 at 1579.402 kb on - strand, within Pf1N1B4_1380 at 1579.402 kb on - strand, within Pf1N1B4_1380 at 1579.402 kb on - strand, within Pf1N1B4_1380 at 1579.434 kb on + strand, within Pf1N1B4_1380 at 1579.434 kb on + strand, within Pf1N1B4_1380 at 1579.434 kb on + strand, within Pf1N1B4_1380
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,576,863 + +0.7 1,576,863 + +0.2 1,576,863 + -1.5 1,576,863 + -0.4 1,576,864 - +0.7 1,576,938 - +0.3 1,577,015 + Pf1N1B4_1378 0.13 +0.9 1,577,016 - Pf1N1B4_1378 0.13 -0.2 1,577,016 - Pf1N1B4_1378 0.13 -2.4 1,577,016 - Pf1N1B4_1378 0.13 -0.5 1,577,076 + Pf1N1B4_1378 0.22 +0.3 1,577,076 + Pf1N1B4_1378 0.22 +0.3 1,577,077 - Pf1N1B4_1378 0.22 -1.1 1,577,303 + Pf1N1B4_1378 0.53 -1.7 1,577,304 - Pf1N1B4_1378 0.53 +0.3 1,577,304 - Pf1N1B4_1378 0.53 -1.2 1,577,411 + Pf1N1B4_1378 0.68 -1.0 1,577,411 + Pf1N1B4_1378 0.68 -2.7 1,577,411 + Pf1N1B4_1378 0.68 -2.5 1,577,411 + Pf1N1B4_1378 0.68 +0.3 1,577,412 - Pf1N1B4_1378 0.68 -0.2 1,577,412 - Pf1N1B4_1378 0.68 +0.3 1,577,412 - Pf1N1B4_1378 0.68 +0.3 1,577,447 + Pf1N1B4_1378 0.73 -2.2 1,577,448 - Pf1N1B4_1378 0.74 -1.6 1,577,595 - -0.7 1,577,631 + -1.0 1,577,632 - -2.2 1,577,637 + -1.7 1,577,637 + +0.3 1,577,638 - -2.0 1,577,638 - -1.7 1,577,638 - -2.5 1,577,638 - -1.7 1,577,740 - Pf1N1B4_1379 0.12 -0.7 1,577,740 - Pf1N1B4_1379 0.12 -0.5 1,577,740 - Pf1N1B4_1379 0.12 +0.3 1,577,760 - Pf1N1B4_1379 0.14 +0.3 1,577,760 - Pf1N1B4_1379 0.14 -2.5 1,577,760 - Pf1N1B4_1379 0.14 -1.7 1,577,766 + Pf1N1B4_1379 0.15 -0.4 1,577,950 + Pf1N1B4_1379 0.36 -1.7 1,578,098 + Pf1N1B4_1379 0.53 -1.7 1,578,098 + Pf1N1B4_1379 0.53 -1.8 1,578,098 + Pf1N1B4_1379 0.53 -0.7 1,578,098 + -1.3 1,578,098 + Pf1N1B4_1379 0.53 -1.4 1,578,098 + Pf1N1B4_1379 0.53 -0.4 1,578,098 + Pf1N1B4_1379 0.53 +0.0 1,578,098 + Pf1N1B4_1379 0.53 -0.7 1,578,098 + Pf1N1B4_1379 0.53 -2.0 1,578,098 + Pf1N1B4_1379 0.53 -0.7 1,578,098 + Pf1N1B4_1379 0.53 -1.3 1,578,098 + Pf1N1B4_1379 0.53 -0.9 1,578,098 + Pf1N1B4_1379 0.53 -1.6 1,578,098 + Pf1N1B4_1379 0.53 -1.0 1,578,098 + Pf1N1B4_1379 0.53 -0.9 1,578,098 + Pf1N1B4_1379 0.53 -2.0 1,578,098 + Pf1N1B4_1379 0.53 +0.7 1,578,098 + Pf1N1B4_1379 0.53 -1.8 1,578,098 + Pf1N1B4_1379 0.53 -0.3 1,578,099 - Pf1N1B4_1379 0.53 -2.5 1,578,099 - Pf1N1B4_1379 0.53 -2.0 1,578,099 - Pf1N1B4_1379 0.53 -2.0 1,578,099 - Pf1N1B4_1379 0.53 -2.5 1,578,099 - Pf1N1B4_1379 0.53 -1.7 1,578,099 - Pf1N1B4_1379 0.53 -0.8 1,578,099 - Pf1N1B4_1379 0.53 +1.3 1,578,099 - Pf1N1B4_1379 0.53 +0.3 1,578,099 - Pf1N1B4_1379 0.53 -1.3 1,578,099 - +0.3 1,578,099 - Pf1N1B4_1379 0.53 +0.0 1,578,099 - Pf1N1B4_1379 0.53 -1.7 1,578,099 - Pf1N1B4_1379 0.53 -2.7 1,578,099 - Pf1N1B4_1379 0.53 -1.3 1,578,099 - Pf1N1B4_1379 0.53 -0.3 1,578,215 - Pf1N1B4_1379 0.67 -1.7 1,578,230 + Pf1N1B4_1379 0.68 -1.1 1,578,343 + Pf1N1B4_1379 0.81 -3.1 1,578,344 - Pf1N1B4_1379 0.81 -2.7 1,578,344 - Pf1N1B4_1379 0.81 -2.0 1,578,344 - Pf1N1B4_1379 0.81 -0.7 1,578,492 + +1.3 1,578,493 - -1.7 1,578,512 + -2.0 1,578,512 + -2.5 1,578,513 - -2.7 1,578,520 + -1.0 1,578,584 + -0.4 1,578,584 + +0.3 1,578,585 - -2.0 1,578,585 - +0.3 1,578,585 - -2.6 1,578,771 + Pf1N1B4_1380 0.21 -0.7 1,578,771 + Pf1N1B4_1380 0.21 -0.7 1,578,772 - Pf1N1B4_1380 0.21 -0.4 1,578,772 - Pf1N1B4_1380 0.21 -1.1 1,578,772 - Pf1N1B4_1380 0.21 -1.7 1,578,772 - Pf1N1B4_1380 0.21 -2.0 1,578,772 - Pf1N1B4_1380 0.21 +0.3 1,578,772 - Pf1N1B4_1380 0.21 -1.2 1,578,776 - Pf1N1B4_1380 0.21 -2.5 1,578,776 - Pf1N1B4_1380 0.21 +1.9 1,578,776 - Pf1N1B4_1380 0.21 +0.3 1,578,776 - Pf1N1B4_1380 0.21 -1.8 1,578,842 + Pf1N1B4_1380 0.26 -0.7 1,578,842 + Pf1N1B4_1380 0.26 +0.3 1,578,842 + Pf1N1B4_1380 0.26 +0.3 1,578,842 + Pf1N1B4_1380 0.26 -2.7 1,578,842 + Pf1N1B4_1380 0.26 -1.3 1,578,843 - Pf1N1B4_1380 0.26 -2.0 1,578,843 - Pf1N1B4_1380 0.26 -2.3 1,578,843 - Pf1N1B4_1380 0.26 -0.7 1,578,843 - Pf1N1B4_1380 0.26 -0.7 1,578,843 - Pf1N1B4_1380 0.26 -1.3 1,578,843 - Pf1N1B4_1380 0.26 -1.7 1,578,843 - Pf1N1B4_1380 0.26 -0.7 1,578,939 + Pf1N1B4_1380 0.34 -0.3 1,578,939 + Pf1N1B4_1380 0.34 -0.7 1,578,939 + Pf1N1B4_1380 0.34 +0.0 1,578,939 + Pf1N1B4_1380 0.34 -3.6 1,578,975 + Pf1N1B4_1380 0.37 -0.7 1,578,975 + Pf1N1B4_1380 0.37 -1.7 1,578,975 + Pf1N1B4_1380 0.37 -0.1 1,578,975 + Pf1N1B4_1380 0.37 -1.3 1,578,975 + Pf1N1B4_1380 0.37 -2.0 1,578,975 + Pf1N1B4_1380 0.37 +0.3 1,578,976 - Pf1N1B4_1380 0.37 -0.3 1,578,976 - Pf1N1B4_1380 0.37 -0.7 1,578,976 - Pf1N1B4_1380 0.37 -0.9 1,578,976 - Pf1N1B4_1380 0.37 -1.4 1,578,976 - +0.3 1,578,976 - Pf1N1B4_1380 0.37 +0.7 1,578,976 - Pf1N1B4_1380 0.37 -1.5 1,578,976 - Pf1N1B4_1380 0.37 -0.7 1,578,977 + Pf1N1B4_1380 0.37 +0.9 1,578,977 + Pf1N1B4_1380 0.37 -2.3 1,578,977 + +1.3 1,578,977 + Pf1N1B4_1380 0.37 -1.3 1,578,978 - Pf1N1B4_1380 0.37 +0.3 1,578,978 - Pf1N1B4_1380 0.37 -1.5 1,578,978 - Pf1N1B4_1380 0.37 -1.7 1,578,978 - Pf1N1B4_1380 0.37 -0.4 1,578,984 + -0.3 1,578,984 + Pf1N1B4_1380 0.37 +0.3 1,578,985 - Pf1N1B4_1380 0.38 -0.7 1,578,985 - Pf1N1B4_1380 0.38 -0.4 1,578,985 - Pf1N1B4_1380 0.38 -1.3 1,578,985 - Pf1N1B4_1380 0.38 +0.3 1,578,985 - Pf1N1B4_1380 0.38 +0.9 1,578,985 - Pf1N1B4_1380 0.38 -0.1 1,578,988 - Pf1N1B4_1380 0.38 -1.4 1,578,988 - +0.3 1,578,988 - Pf1N1B4_1380 0.38 -1.3 1,578,988 - Pf1N1B4_1380 0.38 -2.3 1,578,988 - Pf1N1B4_1380 0.38 -1.3 1,578,988 - Pf1N1B4_1380 0.38 -0.7 1,579,005 + Pf1N1B4_1380 0.39 -1.0 1,579,005 + Pf1N1B4_1380 0.39 -0.3 1,579,006 - Pf1N1B4_1380 0.39 -2.3 1,579,006 - Pf1N1B4_1380 0.39 -0.1 1,579,006 - Pf1N1B4_1380 0.39 -3.3 1,579,006 - Pf1N1B4_1380 0.39 -2.0 1,579,099 + Pf1N1B4_1380 0.46 -0.7 1,579,133 - Pf1N1B4_1380 0.49 -1.3 1,579,133 - Pf1N1B4_1380 0.49 -1.3 1,579,185 + Pf1N1B4_1380 0.53 -3.0 1,579,186 - Pf1N1B4_1380 0.53 -1.7 1,579,205 + Pf1N1B4_1380 0.55 -1.3 1,579,206 - Pf1N1B4_1380 0.55 -1.1 1,579,233 - Pf1N1B4_1380 0.57 -2.3 1,579,287 + Pf1N1B4_1380 0.61 +0.3 1,579,288 - Pf1N1B4_1380 0.61 -1.3 1,579,288 - Pf1N1B4_1380 0.61 +0.3 1,579,326 + Pf1N1B4_1380 0.64 -1.3 1,579,327 - Pf1N1B4_1380 0.64 -1.0 1,579,338 + Pf1N1B4_1380 0.65 -1.0 1,579,401 + Pf1N1B4_1380 0.70 -3.5 1,579,401 + Pf1N1B4_1380 0.70 -1.5 1,579,401 + Pf1N1B4_1380 0.70 -1.0 1,579,401 + Pf1N1B4_1380 0.70 -0.4 1,579,401 + Pf1N1B4_1380 0.70 -2.0 1,579,401 + Pf1N1B4_1380 0.70 -0.7 1,579,402 - Pf1N1B4_1380 0.70 -2.0 1,579,402 - Pf1N1B4_1380 0.70 +1.3 1,579,402 - Pf1N1B4_1380 0.70 -1.0 1,579,402 - Pf1N1B4_1380 0.70 -2.0 1,579,402 - Pf1N1B4_1380 0.70 -0.9 1,579,434 + Pf1N1B4_1380 0.73 -0.9 1,579,434 + Pf1N1B4_1380 0.73 +1.3 1,579,434 + Pf1N1B4_1380 0.73 +0.3
Or see this region's nucleotide sequence