Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1345 and Pf1N1B4_1346 overlap by 4 nucleotides Pf1N1B4_1346 and Pf1N1B4_1347 are separated by 168 nucleotides Pf1N1B4_1347 and Pf1N1B4_1348 are separated by 31 nucleotides
Pf1N1B4_1345 - Urea ABC transporter, permease protein UrtC, at 1,542,250 to 1,543,329
_1345
Pf1N1B4_1346 - Urea ABC transporter, ATPase protein UrtD, at 1,543,326 to 1,544,195
_1346
Pf1N1B4_1347 - Urea ABC transporter, ATPase protein UrtE, at 1,544,364 to 1,545,062
_1347
Pf1N1B4_1348 - Urease accessory protein UreD, at 1,545,094 to 1,545,933
_1348
Position (kb)
1543
1544
1545 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1542.333 kb on - strand at 1542.333 kb on - strand at 1542.333 kb on - strand at 1542.333 kb on - strand at 1542.395 kb on - strand, within Pf1N1B4_1345 at 1542.532 kb on + strand, within Pf1N1B4_1345 at 1542.532 kb on + strand, within Pf1N1B4_1345 at 1542.532 kb on + strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.721 kb on + strand, within Pf1N1B4_1345 at 1542.721 kb on + strand, within Pf1N1B4_1345 at 1542.722 kb on - strand, within Pf1N1B4_1345 at 1542.722 kb on - strand, within Pf1N1B4_1345 at 1543.066 kb on + strand at 1543.066 kb on + strand, within Pf1N1B4_1345 at 1543.066 kb on + strand, within Pf1N1B4_1345 at 1543.067 kb on - strand, within Pf1N1B4_1345 at 1543.067 kb on - strand, within Pf1N1B4_1345 at 1543.087 kb on - strand, within Pf1N1B4_1345 at 1543.234 kb on + strand at 1543.279 kb on + strand at 1543.279 kb on + strand at 1543.279 kb on + strand at 1543.279 kb on + strand at 1543.280 kb on - strand at 1543.280 kb on - strand at 1543.280 kb on - strand at 1543.280 kb on - strand at 1543.287 kb on + strand at 1543.400 kb on + strand at 1543.533 kb on + strand, within Pf1N1B4_1346 at 1543.533 kb on + strand, within Pf1N1B4_1346 at 1543.534 kb on - strand, within Pf1N1B4_1346 at 1543.892 kb on + strand, within Pf1N1B4_1346 at 1543.892 kb on + strand, within Pf1N1B4_1346 at 1543.893 kb on - strand, within Pf1N1B4_1346 at 1543.893 kb on - strand, within Pf1N1B4_1346 at 1544.181 kb on + strand at 1544.181 kb on + strand at 1544.395 kb on - strand at 1544.584 kb on + strand, within Pf1N1B4_1347 at 1544.602 kb on - strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.944 kb on - strand, within Pf1N1B4_1347 at 1544.944 kb on - strand, within Pf1N1B4_1347 at 1544.974 kb on - strand, within Pf1N1B4_1347 at 1544.974 kb on - strand, within Pf1N1B4_1347 at 1545.024 kb on - strand at 1545.024 kb on - strand at 1545.060 kb on + strand at 1545.087 kb on - strand at 1545.087 kb on - strand at 1545.101 kb on + strand at 1545.101 kb on + strand at 1545.101 kb on + strand at 1545.102 kb on - strand at 1545.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,542,333 - -1.3 1,542,333 - +1.9 1,542,333 - -2.0 1,542,333 - +1.3 1,542,395 - Pf1N1B4_1345 0.13 -1.7 1,542,532 + Pf1N1B4_1345 0.26 -1.5 1,542,532 + Pf1N1B4_1345 0.26 -1.1 1,542,532 + Pf1N1B4_1345 0.26 +0.9 1,542,533 - Pf1N1B4_1345 0.26 +0.3 1,542,533 - Pf1N1B4_1345 0.26 +1.3 1,542,533 - Pf1N1B4_1345 0.26 +0.9 1,542,533 - Pf1N1B4_1345 0.26 +0.3 1,542,721 + Pf1N1B4_1345 0.44 -1.1 1,542,721 + Pf1N1B4_1345 0.44 +0.3 1,542,722 - Pf1N1B4_1345 0.44 +1.1 1,542,722 - Pf1N1B4_1345 0.44 +1.9 1,543,066 + +1.3 1,543,066 + Pf1N1B4_1345 0.76 -1.5 1,543,066 + Pf1N1B4_1345 0.76 -1.3 1,543,067 - Pf1N1B4_1345 0.76 +0.3 1,543,067 - Pf1N1B4_1345 0.76 +1.0 1,543,087 - Pf1N1B4_1345 0.78 +0.3 1,543,234 + +1.9 1,543,279 + +1.3 1,543,279 + -0.7 1,543,279 + -1.7 1,543,279 + +0.9 1,543,280 - +1.2 1,543,280 - +0.3 1,543,280 - +0.7 1,543,280 - +1.2 1,543,287 + +1.6 1,543,400 + -1.3 1,543,533 + Pf1N1B4_1346 0.24 +2.3 1,543,533 + Pf1N1B4_1346 0.24 -0.3 1,543,534 - Pf1N1B4_1346 0.24 -0.3 1,543,892 + Pf1N1B4_1346 0.65 +0.7 1,543,892 + Pf1N1B4_1346 0.65 +0.3 1,543,893 - Pf1N1B4_1346 0.65 +1.6 1,543,893 - Pf1N1B4_1346 0.65 -0.3 1,544,181 + +1.0 1,544,181 + -0.7 1,544,395 - +0.8 1,544,584 + Pf1N1B4_1347 0.31 +0.3 1,544,602 - Pf1N1B4_1347 0.34 -2.9 1,544,943 + Pf1N1B4_1347 0.83 -0.1 1,544,943 + Pf1N1B4_1347 0.83 +0.3 1,544,943 + Pf1N1B4_1347 0.83 -0.7 1,544,944 - Pf1N1B4_1347 0.83 +0.3 1,544,944 - Pf1N1B4_1347 0.83 -0.4 1,544,974 - Pf1N1B4_1347 0.87 +0.3 1,544,974 - Pf1N1B4_1347 0.87 +0.3 1,545,024 - -1.3 1,545,024 - -0.7 1,545,060 + -1.3 1,545,087 - -0.1 1,545,087 - +0.7 1,545,101 + +0.3 1,545,101 + -0.3 1,545,101 + -1.7 1,545,102 - +0.6 1,545,102 - +1.3
Or see this region's nucleotide sequence