Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1346

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1345 and Pf1N1B4_1346 overlap by 4 nucleotidesPf1N1B4_1346 and Pf1N1B4_1347 are separated by 168 nucleotidesPf1N1B4_1347 and Pf1N1B4_1348 are separated by 31 nucleotides Pf1N1B4_1345 - Urea ABC transporter, permease protein UrtC, at 1,542,250 to 1,543,329 _1345 Pf1N1B4_1346 - Urea ABC transporter, ATPase protein UrtD, at 1,543,326 to 1,544,195 _1346 Pf1N1B4_1347 - Urea ABC transporter, ATPase protein UrtE, at 1,544,364 to 1,545,062 _1347 Pf1N1B4_1348 - Urease accessory protein UreD, at 1,545,094 to 1,545,933 _1348 Position (kb) 1543 1544 1545Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1542.333 kb on - strandat 1542.333 kb on - strandat 1542.333 kb on - strandat 1542.333 kb on - strandat 1542.395 kb on - strand, within Pf1N1B4_1345at 1542.532 kb on + strand, within Pf1N1B4_1345at 1542.532 kb on + strand, within Pf1N1B4_1345at 1542.532 kb on + strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.721 kb on + strand, within Pf1N1B4_1345at 1542.721 kb on + strand, within Pf1N1B4_1345at 1542.722 kb on - strand, within Pf1N1B4_1345at 1542.722 kb on - strand, within Pf1N1B4_1345at 1543.066 kb on + strandat 1543.066 kb on + strand, within Pf1N1B4_1345at 1543.066 kb on + strand, within Pf1N1B4_1345at 1543.067 kb on - strand, within Pf1N1B4_1345at 1543.067 kb on - strand, within Pf1N1B4_1345at 1543.087 kb on - strand, within Pf1N1B4_1345at 1543.234 kb on + strandat 1543.279 kb on + strandat 1543.279 kb on + strandat 1543.279 kb on + strandat 1543.279 kb on + strandat 1543.280 kb on - strandat 1543.280 kb on - strandat 1543.280 kb on - strandat 1543.280 kb on - strandat 1543.287 kb on + strandat 1543.400 kb on + strandat 1543.533 kb on + strand, within Pf1N1B4_1346at 1543.533 kb on + strand, within Pf1N1B4_1346at 1543.534 kb on - strand, within Pf1N1B4_1346at 1543.892 kb on + strand, within Pf1N1B4_1346at 1543.892 kb on + strand, within Pf1N1B4_1346at 1543.893 kb on - strand, within Pf1N1B4_1346at 1543.893 kb on - strand, within Pf1N1B4_1346at 1544.181 kb on + strandat 1544.181 kb on + strandat 1544.395 kb on - strandat 1544.584 kb on + strand, within Pf1N1B4_1347at 1544.602 kb on - strand, within Pf1N1B4_1347at 1544.943 kb on + strand, within Pf1N1B4_1347at 1544.943 kb on + strand, within Pf1N1B4_1347at 1544.943 kb on + strand, within Pf1N1B4_1347at 1544.944 kb on - strand, within Pf1N1B4_1347at 1544.944 kb on - strand, within Pf1N1B4_1347at 1544.974 kb on - strand, within Pf1N1B4_1347at 1544.974 kb on - strand, within Pf1N1B4_1347at 1545.024 kb on - strandat 1545.024 kb on - strandat 1545.060 kb on + strandat 1545.087 kb on - strandat 1545.087 kb on - strandat 1545.101 kb on + strandat 1545.101 kb on + strandat 1545.101 kb on + strandat 1545.102 kb on - strandat 1545.102 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,542,333 - -1.3
1,542,333 - +1.9
1,542,333 - -2.0
1,542,333 - +1.3
1,542,395 - Pf1N1B4_1345 0.13 -1.7
1,542,532 + Pf1N1B4_1345 0.26 -1.5
1,542,532 + Pf1N1B4_1345 0.26 -1.1
1,542,532 + Pf1N1B4_1345 0.26 +0.9
1,542,533 - Pf1N1B4_1345 0.26 +0.3
1,542,533 - Pf1N1B4_1345 0.26 +1.3
1,542,533 - Pf1N1B4_1345 0.26 +0.9
1,542,533 - Pf1N1B4_1345 0.26 +0.3
1,542,721 + Pf1N1B4_1345 0.44 -1.1
1,542,721 + Pf1N1B4_1345 0.44 +0.3
1,542,722 - Pf1N1B4_1345 0.44 +1.1
1,542,722 - Pf1N1B4_1345 0.44 +1.9
1,543,066 + +1.3
1,543,066 + Pf1N1B4_1345 0.76 -1.5
1,543,066 + Pf1N1B4_1345 0.76 -1.3
1,543,067 - Pf1N1B4_1345 0.76 +0.3
1,543,067 - Pf1N1B4_1345 0.76 +1.0
1,543,087 - Pf1N1B4_1345 0.78 +0.3
1,543,234 + +1.9
1,543,279 + +1.3
1,543,279 + -0.7
1,543,279 + -1.7
1,543,279 + +0.9
1,543,280 - +1.2
1,543,280 - +0.3
1,543,280 - +0.7
1,543,280 - +1.2
1,543,287 + +1.6
1,543,400 + -1.3
1,543,533 + Pf1N1B4_1346 0.24 +2.3
1,543,533 + Pf1N1B4_1346 0.24 -0.3
1,543,534 - Pf1N1B4_1346 0.24 -0.3
1,543,892 + Pf1N1B4_1346 0.65 +0.7
1,543,892 + Pf1N1B4_1346 0.65 +0.3
1,543,893 - Pf1N1B4_1346 0.65 +1.6
1,543,893 - Pf1N1B4_1346 0.65 -0.3
1,544,181 + +1.0
1,544,181 + -0.7
1,544,395 - +0.8
1,544,584 + Pf1N1B4_1347 0.31 +0.3
1,544,602 - Pf1N1B4_1347 0.34 -2.9
1,544,943 + Pf1N1B4_1347 0.83 -0.1
1,544,943 + Pf1N1B4_1347 0.83 +0.3
1,544,943 + Pf1N1B4_1347 0.83 -0.7
1,544,944 - Pf1N1B4_1347 0.83 +0.3
1,544,944 - Pf1N1B4_1347 0.83 -0.4
1,544,974 - Pf1N1B4_1347 0.87 +0.3
1,544,974 - Pf1N1B4_1347 0.87 +0.3
1,545,024 - -1.3
1,545,024 - -0.7
1,545,060 + -1.3
1,545,087 - -0.1
1,545,087 - +0.7
1,545,101 + +0.3
1,545,101 + -0.3
1,545,101 + -1.7
1,545,102 - +0.6
1,545,102 - +1.3

Or see this region's nucleotide sequence