Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1333 and Pf1N1B4_1334 are separated by 272 nucleotides Pf1N1B4_1334 and Pf1N1B4_1335 are separated by 48 nucleotides Pf1N1B4_1335 and Pf1N1B4_1336 are separated by 66 nucleotides Pf1N1B4_1336 and Pf1N1B4_1337 are separated by 84 nucleotides
Pf1N1B4_1333 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 1,528,422 to 1,529,156
_1333
Pf1N1B4_1334 - FIG00955840: hypothetical protein, at 1,529,429 to 1,529,827
_1334
Pf1N1B4_1335 - Copper metallochaperone, bacterial analog of Cox17 protein, at 1,529,876 to 1,530,355
_1335
Pf1N1B4_1336 - Putative multicopper oxidases, at 1,530,422 to 1,530,826
_1336
Pf1N1B4_1337 - Outer membrane receptor proteins, mostly Fe transport, at 1,530,911 to 1,533,028
_1337
Position (kb)
1529
1530
1531 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1529.139 kb on + strand at 1529.139 kb on + strand at 1529.139 kb on + strand at 1529.140 kb on - strand at 1529.140 kb on - strand at 1529.140 kb on - strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.155 kb on - strand at 1529.155 kb on - strand at 1529.155 kb on - strand at 1529.155 kb on - strand at 1529.158 kb on + strand at 1529.158 kb on + strand at 1529.159 kb on - strand at 1529.277 kb on + strand at 1529.278 kb on - strand at 1529.363 kb on + strand at 1529.363 kb on + strand at 1529.364 kb on - strand at 1529.364 kb on - strand at 1529.364 kb on - strand at 1529.364 kb on - strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.386 kb on - strand at 1529.386 kb on - strand at 1529.389 kb on + strand at 1529.390 kb on - strand at 1529.390 kb on - strand at 1529.390 kb on - strand at 1529.717 kb on + strand, within Pf1N1B4_1334 at 1529.717 kb on + strand, within Pf1N1B4_1334 at 1529.717 kb on + strand, within Pf1N1B4_1334 at 1529.718 kb on - strand, within Pf1N1B4_1334 at 1529.721 kb on - strand, within Pf1N1B4_1334 at 1529.774 kb on - strand at 1529.904 kb on + strand at 1529.905 kb on - strand at 1529.905 kb on - strand at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1530.017 kb on + strand, within Pf1N1B4_1335 at 1530.018 kb on - strand, within Pf1N1B4_1335 at 1530.195 kb on - strand, within Pf1N1B4_1335 at 1530.195 kb on - strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.202 kb on - strand at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.340 kb on + strand at 1530.340 kb on + strand at 1530.340 kb on + strand at 1530.340 kb on + strand at 1530.341 kb on - strand at 1530.537 kb on + strand, within Pf1N1B4_1336 at 1530.537 kb on + strand, within Pf1N1B4_1336 at 1530.538 kb on - strand, within Pf1N1B4_1336 at 1530.538 kb on - strand, within Pf1N1B4_1336 at 1530.824 kb on + strand at 1530.888 kb on - strand at 1531.175 kb on + strand, within Pf1N1B4_1337 at 1531.176 kb on - strand, within Pf1N1B4_1337 at 1531.176 kb on - strand, within Pf1N1B4_1337 at 1531.236 kb on + strand, within Pf1N1B4_1337 at 1531.267 kb on + strand, within Pf1N1B4_1337 at 1531.268 kb on - strand, within Pf1N1B4_1337 at 1531.332 kb on - strand, within Pf1N1B4_1337 at 1531.332 kb on - strand, within Pf1N1B4_1337 at 1531.350 kb on - strand, within Pf1N1B4_1337 at 1531.350 kb on - strand, within Pf1N1B4_1337
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,529,139 + -0.7 1,529,139 + +0.0 1,529,139 + -1.2 1,529,140 - -0.7 1,529,140 - +0.9 1,529,140 - -1.0 1,529,154 + +0.3 1,529,154 + +1.9 1,529,154 + -0.2 1,529,154 + -0.7 1,529,154 + -0.4 1,529,154 + +0.0 1,529,155 - +0.3 1,529,155 - +0.7 1,529,155 - -0.7 1,529,155 - -0.7 1,529,158 + -0.7 1,529,158 + -0.3 1,529,159 - -0.3 1,529,277 + -0.5 1,529,278 - +1.9 1,529,363 + -0.1 1,529,363 + +0.3 1,529,364 - +0.3 1,529,364 - +0.0 1,529,364 - -0.1 1,529,364 - +0.7 1,529,385 + +0.9 1,529,385 + +0.6 1,529,385 + -2.1 1,529,385 + -0.3 1,529,385 + +0.0 1,529,385 + -0.5 1,529,386 - -1.5 1,529,386 - -0.5 1,529,389 + +0.3 1,529,390 - +0.9 1,529,390 - +0.3 1,529,390 - +0.9 1,529,717 + Pf1N1B4_1334 0.72 +0.7 1,529,717 + Pf1N1B4_1334 0.72 -0.5 1,529,717 + Pf1N1B4_1334 0.72 -1.7 1,529,718 - Pf1N1B4_1334 0.72 +0.9 1,529,721 - Pf1N1B4_1334 0.73 +2.1 1,529,774 - -0.7 1,529,904 + +0.7 1,529,905 - +1.3 1,529,905 - +0.9 1,529,942 + Pf1N1B4_1335 0.14 -0.3 1,529,942 + Pf1N1B4_1335 0.14 -0.5 1,529,942 + Pf1N1B4_1335 0.14 +0.9 1,529,942 + Pf1N1B4_1335 0.14 -0.2 1,529,942 + Pf1N1B4_1335 0.14 +1.0 1,529,943 - Pf1N1B4_1335 0.14 +0.4 1,529,943 - Pf1N1B4_1335 0.14 +1.4 1,529,943 - Pf1N1B4_1335 0.14 +0.4 1,530,017 + Pf1N1B4_1335 0.29 -0.3 1,530,018 - Pf1N1B4_1335 0.30 +0.3 1,530,195 - Pf1N1B4_1335 0.66 -0.7 1,530,195 - Pf1N1B4_1335 0.66 +1.1 1,530,201 + Pf1N1B4_1335 0.68 +1.7 1,530,201 + Pf1N1B4_1335 0.68 +0.6 1,530,201 + Pf1N1B4_1335 0.68 -0.3 1,530,201 + Pf1N1B4_1335 0.68 -0.3 1,530,201 + Pf1N1B4_1335 0.68 -2.1 1,530,202 - +1.3 1,530,202 - Pf1N1B4_1335 0.68 +0.4 1,530,202 - Pf1N1B4_1335 0.68 +0.5 1,530,202 - Pf1N1B4_1335 0.68 -0.8 1,530,202 - Pf1N1B4_1335 0.68 -1.2 1,530,202 - Pf1N1B4_1335 0.68 +1.2 1,530,340 + -0.3 1,530,340 + +1.3 1,530,340 + -1.8 1,530,340 + -0.2 1,530,341 - -0.7 1,530,537 + Pf1N1B4_1336 0.28 +0.3 1,530,537 + Pf1N1B4_1336 0.28 +0.3 1,530,538 - Pf1N1B4_1336 0.29 -0.7 1,530,538 - Pf1N1B4_1336 0.29 -1.3 1,530,824 + +0.3 1,530,888 - +0.1 1,531,175 + Pf1N1B4_1337 0.12 -0.5 1,531,176 - Pf1N1B4_1337 0.13 -1.0 1,531,176 - Pf1N1B4_1337 0.13 -0.7 1,531,236 + Pf1N1B4_1337 0.15 +0.9 1,531,267 + Pf1N1B4_1337 0.17 +0.3 1,531,268 - Pf1N1B4_1337 0.17 -1.8 1,531,332 - Pf1N1B4_1337 0.20 -0.2 1,531,332 - Pf1N1B4_1337 0.20 +1.6 1,531,350 - Pf1N1B4_1337 0.21 +0.8 1,531,350 - Pf1N1B4_1337 0.21 -1.0
Or see this region's nucleotide sequence