Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1328

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1326 and Pf1N1B4_1327 overlap by 1 nucleotidesPf1N1B4_1327 and Pf1N1B4_1328 overlap by 4 nucleotidesPf1N1B4_1328 and Pf1N1B4_1329 overlap by 8 nucleotides Pf1N1B4_1326 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 1,520,725 to 1,522,428 _1326 Pf1N1B4_1327 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 1,522,428 to 1,523,162 _1327 Pf1N1B4_1328 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 1,523,159 to 1,523,785 _1328 Pf1N1B4_1329 - Cobalamin biosynthesis protein CobG, at 1,523,778 to 1,525,079 _1329 Position (kb) 1523 1524Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1522.263 kb on - strandat 1522.296 kb on - strandat 1522.352 kb on + strandat 1522.471 kb on + strandat 1522.543 kb on + strand, within Pf1N1B4_1327at 1522.543 kb on + strand, within Pf1N1B4_1327at 1522.544 kb on - strand, within Pf1N1B4_1327at 1522.778 kb on - strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strandat 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strandat 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.819 kb on + strand, within Pf1N1B4_1327at 1522.820 kb on - strand, within Pf1N1B4_1327at 1522.903 kb on + strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.928 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.361 kb on + strandat 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.751 kb on + strandat 1523.751 kb on + strandat 1523.751 kb on + strandat 1523.751 kb on + strandat 1523.752 kb on - strandat 1523.752 kb on - strandat 1523.775 kb on + strandat 1523.775 kb on + strandat 1523.775 kb on + strandat 1523.775 kb on + strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.834 kb on + strandat 1523.834 kb on + strandat 1523.834 kb on + strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.837 kb on + strandat 1523.837 kb on + strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.890 kb on + strandat 1523.891 kb on - strandat 1524.283 kb on - strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strandat 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.301 kb on + strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.758 kb on + strand, within Pf1N1B4_1329at 1524.758 kb on + strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,522,263 - -1.7
1,522,296 - -1.5
1,522,352 + -1.6
1,522,471 + -1.1
1,522,543 + Pf1N1B4_1327 0.16 +0.0
1,522,543 + Pf1N1B4_1327 0.16 +0.3
1,522,544 - Pf1N1B4_1327 0.16 +1.9
1,522,778 - Pf1N1B4_1327 0.48 -1.1
1,522,804 + Pf1N1B4_1327 0.51 +0.1
1,522,804 + Pf1N1B4_1327 0.51 -0.7
1,522,804 + +1.9
1,522,804 + Pf1N1B4_1327 0.51 -0.7
1,522,804 + Pf1N1B4_1327 0.51 -0.5
1,522,804 + Pf1N1B4_1327 0.51 +0.3
1,522,805 - Pf1N1B4_1327 0.51 +1.3
1,522,805 - Pf1N1B4_1327 0.51 +0.7
1,522,805 - Pf1N1B4_1327 0.51 +0.3
1,522,805 - Pf1N1B4_1327 0.51 +0.9
1,522,805 - Pf1N1B4_1327 0.51 -0.7
1,522,805 - Pf1N1B4_1327 0.51 -1.3
1,522,805 - Pf1N1B4_1327 0.51 +0.2
1,522,805 - +0.3
1,522,805 - Pf1N1B4_1327 0.51 +0.3
1,522,810 + Pf1N1B4_1327 0.52 -1.1
1,522,810 + Pf1N1B4_1327 0.52 +0.0
1,522,810 + Pf1N1B4_1327 0.52 +0.3
1,522,810 + Pf1N1B4_1327 0.52 -1.7
1,522,810 + Pf1N1B4_1327 0.52 +0.3
1,522,810 + Pf1N1B4_1327 0.52 +1.6
1,522,810 + Pf1N1B4_1327 0.52 -0.7
1,522,810 + Pf1N1B4_1327 0.52 -0.3
1,522,810 + Pf1N1B4_1327 0.52 -1.2
1,522,810 + Pf1N1B4_1327 0.52 +0.3
1,522,810 + Pf1N1B4_1327 0.52 -0.1
1,522,810 + Pf1N1B4_1327 0.52 -2.5
1,522,811 - Pf1N1B4_1327 0.52 -0.5
1,522,811 - Pf1N1B4_1327 0.52 +1.0
1,522,811 - Pf1N1B4_1327 0.52 +0.3
1,522,811 - Pf1N1B4_1327 0.52 +1.3
1,522,811 - Pf1N1B4_1327 0.52 +0.7
1,522,811 - Pf1N1B4_1327 0.52 -1.5
1,522,811 - Pf1N1B4_1327 0.52 -2.7
1,522,811 - Pf1N1B4_1327 0.52 -1.1
1,522,811 - Pf1N1B4_1327 0.52 -1.2
1,522,811 - Pf1N1B4_1327 0.52 -1.1
1,522,811 - Pf1N1B4_1327 0.52 -1.3
1,522,811 - Pf1N1B4_1327 0.52 +0.9
1,522,811 - Pf1N1B4_1327 0.52 -1.7
1,522,819 + Pf1N1B4_1327 0.53 -0.6
1,522,820 - Pf1N1B4_1327 0.53 -0.1
1,522,903 + Pf1N1B4_1327 0.65 +0.3
1,522,904 - Pf1N1B4_1327 0.65 -0.3
1,522,904 - Pf1N1B4_1327 0.65 -0.3
1,522,904 - Pf1N1B4_1327 0.65 -2.4
1,522,904 - Pf1N1B4_1327 0.65 -0.5
1,522,928 - Pf1N1B4_1327 0.68 +0.7
1,523,054 - Pf1N1B4_1327 0.85 -0.7
1,523,054 - Pf1N1B4_1327 0.85 -1.3
1,523,054 - Pf1N1B4_1327 0.85 -0.7
1,523,054 - Pf1N1B4_1327 0.85 -1.1
1,523,361 + +0.7
1,523,361 + Pf1N1B4_1328 0.32 -1.5
1,523,361 + Pf1N1B4_1328 0.32 -0.7
1,523,361 + Pf1N1B4_1328 0.32 -1.3
1,523,362 - Pf1N1B4_1328 0.32 +0.0
1,523,362 - Pf1N1B4_1328 0.32 +0.3
1,523,362 - Pf1N1B4_1328 0.32 -1.5
1,523,362 - Pf1N1B4_1328 0.32 -1.3
1,523,751 + -1.3
1,523,751 + -2.1
1,523,751 + -0.4
1,523,751 + -0.6
1,523,752 - +0.3
1,523,752 - -0.7
1,523,775 + +0.3
1,523,775 + -1.7
1,523,775 + -1.5
1,523,775 + -1.7
1,523,776 - +0.9
1,523,776 - -2.1
1,523,776 - -0.7
1,523,776 - +0.3
1,523,776 - -0.5
1,523,776 - +1.6
1,523,776 - +0.4
1,523,834 + -0.5
1,523,834 + -0.7
1,523,834 + +1.7
1,523,835 - -1.7
1,523,835 - -1.5
1,523,835 - -1.3
1,523,835 - -1.1
1,523,835 - -1.9
1,523,837 + -1.5
1,523,837 + -0.7
1,523,838 - -1.5
1,523,838 - -0.3
1,523,838 - -1.7
1,523,838 - -0.1
1,523,838 - -2.3
1,523,890 + -0.7
1,523,891 - -2.1
1,524,283 - Pf1N1B4_1329 0.39 -0.3
1,524,295 + Pf1N1B4_1329 0.40 +0.3
1,524,295 + Pf1N1B4_1329 0.40 -0.3
1,524,295 + -1.3
1,524,295 + Pf1N1B4_1329 0.40 -2.1
1,524,295 + Pf1N1B4_1329 0.40 -1.7
1,524,295 + Pf1N1B4_1329 0.40 -1.9
1,524,295 + Pf1N1B4_1329 0.40 -0.7
1,524,296 - Pf1N1B4_1329 0.40 -0.1
1,524,296 - Pf1N1B4_1329 0.40 +1.3
1,524,296 - Pf1N1B4_1329 0.40 -1.7
1,524,296 - Pf1N1B4_1329 0.40 -2.9
1,524,296 - Pf1N1B4_1329 0.40 -0.3
1,524,296 - Pf1N1B4_1329 0.40 -0.6
1,524,296 - Pf1N1B4_1329 0.40 -1.7
1,524,296 - Pf1N1B4_1329 0.40 -1.0
1,524,296 - Pf1N1B4_1329 0.40 -2.7
1,524,296 - Pf1N1B4_1329 0.40 -0.5
1,524,296 - Pf1N1B4_1329 0.40 -2.3
1,524,296 - Pf1N1B4_1329 0.40 -1.3
1,524,296 - Pf1N1B4_1329 0.40 -1.2
1,524,301 + Pf1N1B4_1329 0.40 -2.7
1,524,302 - Pf1N1B4_1329 0.40 -0.9
1,524,302 - Pf1N1B4_1329 0.40 +0.3
1,524,302 - Pf1N1B4_1329 0.40 -2.3
1,524,302 - Pf1N1B4_1329 0.40 -0.1
1,524,302 - Pf1N1B4_1329 0.40 -1.3
1,524,758 + Pf1N1B4_1329 0.75 +1.4
1,524,758 + Pf1N1B4_1329 0.75 -1.2
1,524,759 - Pf1N1B4_1329 0.75 -0.1
1,524,759 - Pf1N1B4_1329 0.75 -1.3
1,524,759 - Pf1N1B4_1329 0.75 -0.3
1,524,759 - Pf1N1B4_1329 0.75 -0.5

Or see this region's nucleotide sequence