Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1326 and Pf1N1B4_1327 overlap by 1 nucleotides Pf1N1B4_1327 and Pf1N1B4_1328 overlap by 4 nucleotides Pf1N1B4_1328 and Pf1N1B4_1329 overlap by 8 nucleotides
Pf1N1B4_1326 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 1,520,725 to 1,522,428
_1326
Pf1N1B4_1327 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 1,522,428 to 1,523,162
_1327
Pf1N1B4_1328 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 1,523,159 to 1,523,785
_1328
Pf1N1B4_1329 - Cobalamin biosynthesis protein CobG, at 1,523,778 to 1,525,079
_1329
Position (kb)
1523
1524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1522.263 kb on - strand at 1522.296 kb on - strand at 1522.352 kb on + strand at 1522.471 kb on + strand at 1522.543 kb on + strand, within Pf1N1B4_1327 at 1522.543 kb on + strand, within Pf1N1B4_1327 at 1522.544 kb on - strand, within Pf1N1B4_1327 at 1522.778 kb on - strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.819 kb on + strand, within Pf1N1B4_1327 at 1522.820 kb on - strand, within Pf1N1B4_1327 at 1522.903 kb on + strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.928 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.361 kb on + strand at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.751 kb on + strand at 1523.751 kb on + strand at 1523.751 kb on + strand at 1523.751 kb on + strand at 1523.752 kb on - strand at 1523.752 kb on - strand at 1523.775 kb on + strand at 1523.775 kb on + strand at 1523.775 kb on + strand at 1523.775 kb on + strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.834 kb on + strand at 1523.834 kb on + strand at 1523.834 kb on + strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.837 kb on + strand at 1523.837 kb on + strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.890 kb on + strand at 1523.891 kb on - strand at 1524.283 kb on - strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.301 kb on + strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,522,263 - -1.7 1,522,296 - -1.5 1,522,352 + -1.6 1,522,471 + -1.1 1,522,543 + Pf1N1B4_1327 0.16 +0.0 1,522,543 + Pf1N1B4_1327 0.16 +0.3 1,522,544 - Pf1N1B4_1327 0.16 +1.9 1,522,778 - Pf1N1B4_1327 0.48 -1.1 1,522,804 + Pf1N1B4_1327 0.51 +0.1 1,522,804 + Pf1N1B4_1327 0.51 -0.7 1,522,804 + +1.9 1,522,804 + Pf1N1B4_1327 0.51 -0.7 1,522,804 + Pf1N1B4_1327 0.51 -0.5 1,522,804 + Pf1N1B4_1327 0.51 +0.3 1,522,805 - Pf1N1B4_1327 0.51 +1.3 1,522,805 - Pf1N1B4_1327 0.51 +0.7 1,522,805 - Pf1N1B4_1327 0.51 +0.3 1,522,805 - Pf1N1B4_1327 0.51 +0.9 1,522,805 - Pf1N1B4_1327 0.51 -0.7 1,522,805 - Pf1N1B4_1327 0.51 -1.3 1,522,805 - Pf1N1B4_1327 0.51 +0.2 1,522,805 - +0.3 1,522,805 - Pf1N1B4_1327 0.51 +0.3 1,522,810 + Pf1N1B4_1327 0.52 -1.1 1,522,810 + Pf1N1B4_1327 0.52 +0.0 1,522,810 + Pf1N1B4_1327 0.52 +0.3 1,522,810 + Pf1N1B4_1327 0.52 -1.7 1,522,810 + Pf1N1B4_1327 0.52 +0.3 1,522,810 + Pf1N1B4_1327 0.52 +1.6 1,522,810 + Pf1N1B4_1327 0.52 -0.7 1,522,810 + Pf1N1B4_1327 0.52 -0.3 1,522,810 + Pf1N1B4_1327 0.52 -1.2 1,522,810 + Pf1N1B4_1327 0.52 +0.3 1,522,810 + Pf1N1B4_1327 0.52 -0.1 1,522,810 + Pf1N1B4_1327 0.52 -2.5 1,522,811 - Pf1N1B4_1327 0.52 -0.5 1,522,811 - Pf1N1B4_1327 0.52 +1.0 1,522,811 - Pf1N1B4_1327 0.52 +0.3 1,522,811 - Pf1N1B4_1327 0.52 +1.3 1,522,811 - Pf1N1B4_1327 0.52 +0.7 1,522,811 - Pf1N1B4_1327 0.52 -1.5 1,522,811 - Pf1N1B4_1327 0.52 -2.7 1,522,811 - Pf1N1B4_1327 0.52 -1.1 1,522,811 - Pf1N1B4_1327 0.52 -1.2 1,522,811 - Pf1N1B4_1327 0.52 -1.1 1,522,811 - Pf1N1B4_1327 0.52 -1.3 1,522,811 - Pf1N1B4_1327 0.52 +0.9 1,522,811 - Pf1N1B4_1327 0.52 -1.7 1,522,819 + Pf1N1B4_1327 0.53 -0.6 1,522,820 - Pf1N1B4_1327 0.53 -0.1 1,522,903 + Pf1N1B4_1327 0.65 +0.3 1,522,904 - Pf1N1B4_1327 0.65 -0.3 1,522,904 - Pf1N1B4_1327 0.65 -0.3 1,522,904 - Pf1N1B4_1327 0.65 -2.4 1,522,904 - Pf1N1B4_1327 0.65 -0.5 1,522,928 - Pf1N1B4_1327 0.68 +0.7 1,523,054 - Pf1N1B4_1327 0.85 -0.7 1,523,054 - Pf1N1B4_1327 0.85 -1.3 1,523,054 - Pf1N1B4_1327 0.85 -0.7 1,523,054 - Pf1N1B4_1327 0.85 -1.1 1,523,361 + +0.7 1,523,361 + Pf1N1B4_1328 0.32 -1.5 1,523,361 + Pf1N1B4_1328 0.32 -0.7 1,523,361 + Pf1N1B4_1328 0.32 -1.3 1,523,362 - Pf1N1B4_1328 0.32 +0.0 1,523,362 - Pf1N1B4_1328 0.32 +0.3 1,523,362 - Pf1N1B4_1328 0.32 -1.5 1,523,362 - Pf1N1B4_1328 0.32 -1.3 1,523,751 + -1.3 1,523,751 + -2.1 1,523,751 + -0.4 1,523,751 + -0.6 1,523,752 - +0.3 1,523,752 - -0.7 1,523,775 + +0.3 1,523,775 + -1.7 1,523,775 + -1.5 1,523,775 + -1.7 1,523,776 - +0.9 1,523,776 - -2.1 1,523,776 - -0.7 1,523,776 - +0.3 1,523,776 - -0.5 1,523,776 - +1.6 1,523,776 - +0.4 1,523,834 + -0.5 1,523,834 + -0.7 1,523,834 + +1.7 1,523,835 - -1.7 1,523,835 - -1.5 1,523,835 - -1.3 1,523,835 - -1.1 1,523,835 - -1.9 1,523,837 + -1.5 1,523,837 + -0.7 1,523,838 - -1.5 1,523,838 - -0.3 1,523,838 - -1.7 1,523,838 - -0.1 1,523,838 - -2.3 1,523,890 + -0.7 1,523,891 - -2.1 1,524,283 - Pf1N1B4_1329 0.39 -0.3 1,524,295 + Pf1N1B4_1329 0.40 +0.3 1,524,295 + Pf1N1B4_1329 0.40 -0.3 1,524,295 + -1.3 1,524,295 + Pf1N1B4_1329 0.40 -2.1 1,524,295 + Pf1N1B4_1329 0.40 -1.7 1,524,295 + Pf1N1B4_1329 0.40 -1.9 1,524,295 + Pf1N1B4_1329 0.40 -0.7 1,524,296 - Pf1N1B4_1329 0.40 -0.1 1,524,296 - Pf1N1B4_1329 0.40 +1.3 1,524,296 - Pf1N1B4_1329 0.40 -1.7 1,524,296 - Pf1N1B4_1329 0.40 -2.9 1,524,296 - Pf1N1B4_1329 0.40 -0.3 1,524,296 - Pf1N1B4_1329 0.40 -0.6 1,524,296 - Pf1N1B4_1329 0.40 -1.7 1,524,296 - Pf1N1B4_1329 0.40 -1.0 1,524,296 - Pf1N1B4_1329 0.40 -2.7 1,524,296 - Pf1N1B4_1329 0.40 -0.5 1,524,296 - Pf1N1B4_1329 0.40 -2.3 1,524,296 - Pf1N1B4_1329 0.40 -1.3 1,524,296 - Pf1N1B4_1329 0.40 -1.2 1,524,301 + Pf1N1B4_1329 0.40 -2.7 1,524,302 - Pf1N1B4_1329 0.40 -0.9 1,524,302 - Pf1N1B4_1329 0.40 +0.3 1,524,302 - Pf1N1B4_1329 0.40 -2.3 1,524,302 - Pf1N1B4_1329 0.40 -0.1 1,524,302 - Pf1N1B4_1329 0.40 -1.3 1,524,758 + Pf1N1B4_1329 0.75 +1.4 1,524,758 + Pf1N1B4_1329 0.75 -1.2 1,524,759 - Pf1N1B4_1329 0.75 -0.1 1,524,759 - Pf1N1B4_1329 0.75 -1.3 1,524,759 - Pf1N1B4_1329 0.75 -0.3 1,524,759 - Pf1N1B4_1329 0.75 -0.5
Or see this region's nucleotide sequence