Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1317

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1316 and Pf1N1B4_1317 are separated by 99 nucleotidesPf1N1B4_1317 and Pf1N1B4_1318 are separated by 49 nucleotidesPf1N1B4_1318 and Pf1N1B4_1319 are separated by 86 nucleotides Pf1N1B4_1316 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 1,499,196 to 1,500,491 _1316 Pf1N1B4_1317 - Sensor histidine kinase/response regulator, at 1,500,591 to 1,503,365 _1317 Pf1N1B4_1318 - hypothetical protein, at 1,503,415 to 1,504,077 _1318 Pf1N1B4_1319 - hypothetical protein, at 1,504,164 to 1,504,949 _1319 Position (kb) 1500 1501 1502 1503 1504Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1499.592 kb on + strand, within Pf1N1B4_1316at 1499.593 kb on - strand, within Pf1N1B4_1316at 1499.616 kb on - strand, within Pf1N1B4_1316at 1499.809 kb on + strand, within Pf1N1B4_1316at 1500.489 kb on + strandat 1500.489 kb on + strandat 1500.490 kb on - strandat 1500.510 kb on + strandat 1500.536 kb on - strandat 1500.552 kb on + strandat 1500.552 kb on + strandat 1500.552 kb on + strandat 1500.552 kb on + strandat 1500.553 kb on - strandat 1500.553 kb on - strandat 1500.553 kb on - strandat 1500.556 kb on + strandat 1500.556 kb on + strandat 1500.556 kb on + strandat 1500.557 kb on - strandat 1500.557 kb on - strandat 1500.558 kb on + strandat 1500.558 kb on + strandat 1500.558 kb on + strandat 1500.559 kb on - strandat 1500.574 kb on + strandat 1500.574 kb on + strandat 1500.574 kb on + strandat 1500.574 kb on + strandat 1500.574 kb on + strandat 1500.574 kb on + strandat 1500.575 kb on - strandat 1500.575 kb on - strandat 1500.575 kb on - strandat 1500.888 kb on + strand, within Pf1N1B4_1317at 1501.053 kb on + strand, within Pf1N1B4_1317at 1501.466 kb on + strand, within Pf1N1B4_1317at 1501.471 kb on - strand, within Pf1N1B4_1317at 1501.894 kb on - strand, within Pf1N1B4_1317at 1502.894 kb on + strand, within Pf1N1B4_1317at 1503.007 kb on + strand, within Pf1N1B4_1317at 1503.413 kb on + strandat 1503.414 kb on - strandat 1503.414 kb on - strandat 1503.414 kb on - strandat 1503.414 kb on - strandat 1503.562 kb on + strand, within Pf1N1B4_1318at 1503.563 kb on - strandat 1503.563 kb on - strand, within Pf1N1B4_1318at 1503.633 kb on - strand, within Pf1N1B4_1318at 1503.761 kb on + strand, within Pf1N1B4_1318at 1503.762 kb on - strand, within Pf1N1B4_1318at 1503.762 kb on - strand, within Pf1N1B4_1318at 1503.762 kb on - strand, within Pf1N1B4_1318at 1503.762 kb on - strand, within Pf1N1B4_1318at 1503.762 kb on - strand, within Pf1N1B4_1318at 1503.783 kb on - strand, within Pf1N1B4_1318at 1503.823 kb on - strand, within Pf1N1B4_1318at 1503.823 kb on - strand, within Pf1N1B4_1318at 1503.938 kb on + strand, within Pf1N1B4_1318at 1503.938 kb on + strand, within Pf1N1B4_1318at 1503.939 kb on - strand, within Pf1N1B4_1318at 1503.939 kb on - strand, within Pf1N1B4_1318at 1504.015 kb on + strandat 1504.016 kb on - strandat 1504.016 kb on - strandat 1504.093 kb on + strandat 1504.093 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,499,592 + Pf1N1B4_1316 0.31 -1.3
1,499,593 - Pf1N1B4_1316 0.31 -0.7
1,499,616 - Pf1N1B4_1316 0.32 +0.3
1,499,809 + Pf1N1B4_1316 0.47 -2.5
1,500,489 + -2.1
1,500,489 + +0.3
1,500,490 - -3.2
1,500,510 + -0.7
1,500,536 - -0.1
1,500,552 + +0.3
1,500,552 + -0.3
1,500,552 + -1.7
1,500,552 + +0.3
1,500,553 - -2.9
1,500,553 - +0.3
1,500,553 - -1.7
1,500,556 + -0.1
1,500,556 + -0.7
1,500,556 + +1.3
1,500,557 - -1.9
1,500,557 - +0.3
1,500,558 + -0.1
1,500,558 + +1.3
1,500,558 + -0.3
1,500,559 - -1.7
1,500,574 + -0.1
1,500,574 + -0.1
1,500,574 + -0.0
1,500,574 + +0.3
1,500,574 + -0.7
1,500,574 + +0.8
1,500,575 - -1.3
1,500,575 - -1.3
1,500,575 - -0.7
1,500,888 + Pf1N1B4_1317 0.11 -2.9
1,501,053 + Pf1N1B4_1317 0.17 -0.3
1,501,466 + Pf1N1B4_1317 0.32 +0.3
1,501,471 - Pf1N1B4_1317 0.32 -1.5
1,501,894 - Pf1N1B4_1317 0.47 -1.3
1,502,894 + Pf1N1B4_1317 0.83 +0.3
1,503,007 + Pf1N1B4_1317 0.87 -1.7
1,503,413 + +0.3
1,503,414 - -0.3
1,503,414 - +0.7
1,503,414 - -0.7
1,503,414 - -1.3
1,503,562 + Pf1N1B4_1318 0.22 +0.1
1,503,563 - -0.3
1,503,563 - Pf1N1B4_1318 0.22 +0.3
1,503,633 - Pf1N1B4_1318 0.33 +1.8
1,503,761 + Pf1N1B4_1318 0.52 +0.6
1,503,762 - Pf1N1B4_1318 0.52 +0.8
1,503,762 - Pf1N1B4_1318 0.52 -1.2
1,503,762 - Pf1N1B4_1318 0.52 -0.7
1,503,762 - Pf1N1B4_1318 0.52 +1.3
1,503,762 - Pf1N1B4_1318 0.52 -0.3
1,503,783 - Pf1N1B4_1318 0.56 -0.3
1,503,823 - Pf1N1B4_1318 0.62 -1.2
1,503,823 - Pf1N1B4_1318 0.62 -1.1
1,503,938 + Pf1N1B4_1318 0.79 +0.3
1,503,938 + Pf1N1B4_1318 0.79 -0.7
1,503,939 - Pf1N1B4_1318 0.79 -0.1
1,503,939 - Pf1N1B4_1318 0.79 -0.5
1,504,015 + +0.3
1,504,016 - +0.8
1,504,016 - -0.4
1,504,093 + +2.6
1,504,093 + -2.3

Or see this region's nucleotide sequence