Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1316 and Pf1N1B4_1317 are separated by 99 nucleotides Pf1N1B4_1317 and Pf1N1B4_1318 are separated by 49 nucleotides Pf1N1B4_1318 and Pf1N1B4_1319 are separated by 86 nucleotides
Pf1N1B4_1316 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 1,499,196 to 1,500,491
_1316
Pf1N1B4_1317 - Sensor histidine kinase/response regulator, at 1,500,591 to 1,503,365
_1317
Pf1N1B4_1318 - hypothetical protein, at 1,503,415 to 1,504,077
_1318
Pf1N1B4_1319 - hypothetical protein, at 1,504,164 to 1,504,949
_1319
Position (kb)
1500
1501
1502
1503
1504 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1499.592 kb on + strand, within Pf1N1B4_1316 at 1499.593 kb on - strand, within Pf1N1B4_1316 at 1499.616 kb on - strand, within Pf1N1B4_1316 at 1499.809 kb on + strand, within Pf1N1B4_1316 at 1500.489 kb on + strand at 1500.489 kb on + strand at 1500.490 kb on - strand at 1500.510 kb on + strand at 1500.536 kb on - strand at 1500.552 kb on + strand at 1500.552 kb on + strand at 1500.552 kb on + strand at 1500.552 kb on + strand at 1500.553 kb on - strand at 1500.553 kb on - strand at 1500.553 kb on - strand at 1500.556 kb on + strand at 1500.556 kb on + strand at 1500.556 kb on + strand at 1500.557 kb on - strand at 1500.557 kb on - strand at 1500.558 kb on + strand at 1500.558 kb on + strand at 1500.558 kb on + strand at 1500.559 kb on - strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.574 kb on + strand at 1500.575 kb on - strand at 1500.575 kb on - strand at 1500.575 kb on - strand at 1500.888 kb on + strand, within Pf1N1B4_1317 at 1501.053 kb on + strand, within Pf1N1B4_1317 at 1501.466 kb on + strand, within Pf1N1B4_1317 at 1501.471 kb on - strand, within Pf1N1B4_1317 at 1501.894 kb on - strand, within Pf1N1B4_1317 at 1502.894 kb on + strand, within Pf1N1B4_1317 at 1503.007 kb on + strand, within Pf1N1B4_1317 at 1503.413 kb on + strand at 1503.414 kb on - strand at 1503.414 kb on - strand at 1503.414 kb on - strand at 1503.414 kb on - strand at 1503.562 kb on + strand, within Pf1N1B4_1318 at 1503.563 kb on - strand at 1503.563 kb on - strand, within Pf1N1B4_1318 at 1503.633 kb on - strand, within Pf1N1B4_1318 at 1503.761 kb on + strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.762 kb on - strand, within Pf1N1B4_1318 at 1503.783 kb on - strand, within Pf1N1B4_1318 at 1503.823 kb on - strand, within Pf1N1B4_1318 at 1503.823 kb on - strand, within Pf1N1B4_1318 at 1503.938 kb on + strand, within Pf1N1B4_1318 at 1503.938 kb on + strand, within Pf1N1B4_1318 at 1503.939 kb on - strand, within Pf1N1B4_1318 at 1503.939 kb on - strand, within Pf1N1B4_1318 at 1504.015 kb on + strand at 1504.016 kb on - strand at 1504.016 kb on - strand at 1504.093 kb on + strand at 1504.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,499,592 + Pf1N1B4_1316 0.31 -1.3 1,499,593 - Pf1N1B4_1316 0.31 -0.7 1,499,616 - Pf1N1B4_1316 0.32 +0.3 1,499,809 + Pf1N1B4_1316 0.47 -2.5 1,500,489 + -2.1 1,500,489 + +0.3 1,500,490 - -3.2 1,500,510 + -0.7 1,500,536 - -0.1 1,500,552 + +0.3 1,500,552 + -0.3 1,500,552 + -1.7 1,500,552 + +0.3 1,500,553 - -2.9 1,500,553 - +0.3 1,500,553 - -1.7 1,500,556 + -0.1 1,500,556 + -0.7 1,500,556 + +1.3 1,500,557 - -1.9 1,500,557 - +0.3 1,500,558 + -0.1 1,500,558 + +1.3 1,500,558 + -0.3 1,500,559 - -1.7 1,500,574 + -0.1 1,500,574 + -0.1 1,500,574 + -0.0 1,500,574 + +0.3 1,500,574 + -0.7 1,500,574 + +0.8 1,500,575 - -1.3 1,500,575 - -1.3 1,500,575 - -0.7 1,500,888 + Pf1N1B4_1317 0.11 -2.9 1,501,053 + Pf1N1B4_1317 0.17 -0.3 1,501,466 + Pf1N1B4_1317 0.32 +0.3 1,501,471 - Pf1N1B4_1317 0.32 -1.5 1,501,894 - Pf1N1B4_1317 0.47 -1.3 1,502,894 + Pf1N1B4_1317 0.83 +0.3 1,503,007 + Pf1N1B4_1317 0.87 -1.7 1,503,413 + +0.3 1,503,414 - -0.3 1,503,414 - +0.7 1,503,414 - -0.7 1,503,414 - -1.3 1,503,562 + Pf1N1B4_1318 0.22 +0.1 1,503,563 - -0.3 1,503,563 - Pf1N1B4_1318 0.22 +0.3 1,503,633 - Pf1N1B4_1318 0.33 +1.8 1,503,761 + Pf1N1B4_1318 0.52 +0.6 1,503,762 - Pf1N1B4_1318 0.52 +0.8 1,503,762 - Pf1N1B4_1318 0.52 -1.2 1,503,762 - Pf1N1B4_1318 0.52 -0.7 1,503,762 - Pf1N1B4_1318 0.52 +1.3 1,503,762 - Pf1N1B4_1318 0.52 -0.3 1,503,783 - Pf1N1B4_1318 0.56 -0.3 1,503,823 - Pf1N1B4_1318 0.62 -1.2 1,503,823 - Pf1N1B4_1318 0.62 -1.1 1,503,938 + Pf1N1B4_1318 0.79 +0.3 1,503,938 + Pf1N1B4_1318 0.79 -0.7 1,503,939 - Pf1N1B4_1318 0.79 -0.1 1,503,939 - Pf1N1B4_1318 0.79 -0.5 1,504,015 + +0.3 1,504,016 - +0.8 1,504,016 - -0.4 1,504,093 + +2.6 1,504,093 + -2.3
Or see this region's nucleotide sequence