Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1306 and Pf1N1B4_1307 are separated by 132 nucleotides Pf1N1B4_1307 and Pf1N1B4_1308 are separated by 49 nucleotides Pf1N1B4_1308 and Pf1N1B4_1309 are separated by 17 nucleotides
Pf1N1B4_1306 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 1,489,168 to 1,490,907
_1306
Pf1N1B4_1307 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 1,491,040 to 1,491,492
_1307
Pf1N1B4_1308 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 1,491,542 to 1,492,009
_1308
Pf1N1B4_1309 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 1,492,027 to 1,493,385
_1309
Position (kb)
1491
1492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1490.072 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.359 kb on - strand, within Pf1N1B4_1306 at 1490.380 kb on + strand, within Pf1N1B4_1306 at 1490.380 kb on + strand, within Pf1N1B4_1306 at 1490.380 kb on + strand, within Pf1N1B4_1306 at 1490.381 kb on - strand, within Pf1N1B4_1306 at 1490.381 kb on - strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.383 kb on - strand, within Pf1N1B4_1306 at 1490.383 kb on - strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.453 kb on - strand, within Pf1N1B4_1306 at 1490.557 kb on - strand, within Pf1N1B4_1306 at 1490.773 kb on + strand at 1490.773 kb on + strand at 1490.774 kb on - strand at 1490.774 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.921 kb on + strand at 1490.921 kb on + strand at 1491.059 kb on + strand at 1491.182 kb on - strand, within Pf1N1B4_1307 at 1491.282 kb on + strand, within Pf1N1B4_1307 at 1491.385 kb on + strand, within Pf1N1B4_1307 at 1491.385 kb on + strand, within Pf1N1B4_1307 at 1491.385 kb on + strand, within Pf1N1B4_1307
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,490,072 + Pf1N1B4_1306 0.52 +0.4 1,490,101 + Pf1N1B4_1306 0.54 -1.3 1,490,101 + Pf1N1B4_1306 0.54 -0.2 1,490,101 + Pf1N1B4_1306 0.54 +1.1 1,490,101 + Pf1N1B4_1306 0.54 +0.2 1,490,101 + Pf1N1B4_1306 0.54 -1.0 1,490,101 + Pf1N1B4_1306 0.54 -0.4 1,490,101 + Pf1N1B4_1306 0.54 +0.1 1,490,101 + Pf1N1B4_1306 0.54 -1.9 1,490,101 + Pf1N1B4_1306 0.54 -0.9 1,490,101 + Pf1N1B4_1306 0.54 +1.2 1,490,101 + Pf1N1B4_1306 0.54 -0.4 1,490,101 + Pf1N1B4_1306 0.54 -0.1 1,490,101 + Pf1N1B4_1306 0.54 -0.6 1,490,101 + Pf1N1B4_1306 0.54 +0.1 1,490,101 + Pf1N1B4_1306 0.54 -0.8 1,490,102 - Pf1N1B4_1306 0.54 -0.5 1,490,102 - Pf1N1B4_1306 0.54 -1.4 1,490,102 - Pf1N1B4_1306 0.54 -0.3 1,490,102 - Pf1N1B4_1306 0.54 +0.7 1,490,102 - Pf1N1B4_1306 0.54 +3.1 1,490,102 - Pf1N1B4_1306 0.54 +0.3 1,490,102 - Pf1N1B4_1306 0.54 +0.3 1,490,102 - Pf1N1B4_1306 0.54 -0.3 1,490,102 - Pf1N1B4_1306 0.54 +0.5 1,490,236 + Pf1N1B4_1306 0.61 +1.8 1,490,236 + Pf1N1B4_1306 0.61 +1.0 1,490,236 + Pf1N1B4_1306 0.61 -1.3 1,490,236 + Pf1N1B4_1306 0.61 +0.3 1,490,236 + Pf1N1B4_1306 0.61 +0.3 1,490,236 + Pf1N1B4_1306 0.61 -0.3 1,490,236 + Pf1N1B4_1306 0.61 +0.3 1,490,236 + Pf1N1B4_1306 0.61 -0.7 1,490,237 - Pf1N1B4_1306 0.61 +1.3 1,490,237 - Pf1N1B4_1306 0.61 -0.5 1,490,237 - Pf1N1B4_1306 0.61 +1.3 1,490,237 - Pf1N1B4_1306 0.61 +2.3 1,490,359 - Pf1N1B4_1306 0.68 +0.8 1,490,380 + Pf1N1B4_1306 0.70 +0.8 1,490,380 + Pf1N1B4_1306 0.70 +0.3 1,490,380 + Pf1N1B4_1306 0.70 -0.3 1,490,381 - Pf1N1B4_1306 0.70 +0.3 1,490,381 - Pf1N1B4_1306 0.70 -0.1 1,490,382 + Pf1N1B4_1306 0.70 +0.8 1,490,382 + Pf1N1B4_1306 0.70 +1.8 1,490,382 + Pf1N1B4_1306 0.70 +0.5 1,490,382 + Pf1N1B4_1306 0.70 +1.3 1,490,383 - Pf1N1B4_1306 0.70 +3.3 1,490,383 - Pf1N1B4_1306 0.70 +1.3 1,490,434 + Pf1N1B4_1306 0.73 +1.4 1,490,434 + Pf1N1B4_1306 0.73 -1.1 1,490,434 + Pf1N1B4_1306 0.73 -0.2 1,490,434 + Pf1N1B4_1306 0.73 +1.5 1,490,435 - Pf1N1B4_1306 0.73 -2.7 1,490,435 - Pf1N1B4_1306 0.73 -0.7 1,490,435 - Pf1N1B4_1306 0.73 -0.2 1,490,435 - Pf1N1B4_1306 0.73 -1.1 1,490,435 - Pf1N1B4_1306 0.73 -0.3 1,490,435 - Pf1N1B4_1306 0.73 -1.7 1,490,453 - Pf1N1B4_1306 0.74 +0.7 1,490,557 - Pf1N1B4_1306 0.80 -0.3 1,490,773 + +1.8 1,490,773 + -0.1 1,490,774 - +0.7 1,490,774 - -0.9 1,490,781 - +0.3 1,490,781 - -0.9 1,490,781 - +1.1 1,490,781 - -0.7 1,490,781 - -1.7 1,490,781 - +0.3 1,490,781 - +0.6 1,490,781 - +0.0 1,490,921 + -0.0 1,490,921 + -0.0 1,491,059 + -1.3 1,491,182 - Pf1N1B4_1307 0.31 +0.3 1,491,282 + Pf1N1B4_1307 0.53 -3.1 1,491,385 + Pf1N1B4_1307 0.76 -0.7 1,491,385 + Pf1N1B4_1307 0.76 -2.9 1,491,385 + Pf1N1B4_1307 0.76 -3.3
Or see this region's nucleotide sequence