Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1293

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1292 and Pf1N1B4_1293 are separated by 19 nucleotidesPf1N1B4_1293 and Pf1N1B4_1294 overlap by 4 nucleotides Pf1N1B4_1292 - DNA-damage-inducible protein F, at 1,472,913 to 1,474,253 _1292 Pf1N1B4_1293 - hypothetical protein, at 1,474,273 to 1,475,070 _1293 Pf1N1B4_1294 - VgrG protein, at 1,475,067 to 1,476,122 _1294 Position (kb) 1474 1475 1476Strain fitness (log2 ratio) -2 -1 0 1 2at 1473.803 kb on - strandat 1473.803 kb on - strand, within Pf1N1B4_1292at 1473.803 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.986 kb on - strand, within Pf1N1B4_1292at 1473.987 kb on + strand, within Pf1N1B4_1292at 1473.987 kb on + strand, within Pf1N1B4_1292at 1473.988 kb on - strand, within Pf1N1B4_1292at 1473.996 kb on + strand, within Pf1N1B4_1292at 1473.997 kb on - strand, within Pf1N1B4_1292at 1474.032 kb on + strand, within Pf1N1B4_1292at 1474.032 kb on + strand, within Pf1N1B4_1292at 1474.032 kb on + strand, within Pf1N1B4_1292at 1474.033 kb on - strand, within Pf1N1B4_1292at 1474.088 kb on + strandat 1474.089 kb on - strand, within Pf1N1B4_1292at 1474.218 kb on + strandat 1474.218 kb on + strandat 1474.219 kb on - strandat 1474.219 kb on - strandat 1474.442 kb on + strand, within Pf1N1B4_1293at 1474.442 kb on + strand, within Pf1N1B4_1293at 1474.443 kb on - strand, within Pf1N1B4_1293at 1474.443 kb on - strand, within Pf1N1B4_1293at 1474.460 kb on + strandat 1474.461 kb on - strand, within Pf1N1B4_1293at 1474.712 kb on + strand, within Pf1N1B4_1293at 1474.712 kb on + strandat 1474.733 kb on + strand, within Pf1N1B4_1293at 1474.733 kb on + strand, within Pf1N1B4_1293at 1474.733 kb on + strand, within Pf1N1B4_1293at 1474.734 kb on - strand, within Pf1N1B4_1293at 1474.736 kb on + strand, within Pf1N1B4_1293at 1475.601 kb on - strand, within Pf1N1B4_1294at 1475.602 kb on + strand, within Pf1N1B4_1294at 1475.603 kb on - strand, within Pf1N1B4_1294at 1475.890 kb on + strand, within Pf1N1B4_1294at 1476.030 kb on + strandat 1476.031 kb on - strandat 1476.031 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,473,803 - -0.7
1,473,803 - Pf1N1B4_1292 0.66 -1.9
1,473,803 - Pf1N1B4_1292 0.66 +0.5
1,473,986 - Pf1N1B4_1292 0.80 -0.7
1,473,986 - Pf1N1B4_1292 0.80 -1.1
1,473,986 - Pf1N1B4_1292 0.80 +0.3
1,473,986 - Pf1N1B4_1292 0.80 -1.3
1,473,986 - Pf1N1B4_1292 0.80 -1.3
1,473,986 - Pf1N1B4_1292 0.80 +0.8
1,473,986 - Pf1N1B4_1292 0.80 +2.3
1,473,987 + Pf1N1B4_1292 0.80 -0.6
1,473,987 + Pf1N1B4_1292 0.80 +1.8
1,473,988 - Pf1N1B4_1292 0.80 -0.1
1,473,996 + Pf1N1B4_1292 0.81 +0.7
1,473,997 - Pf1N1B4_1292 0.81 -0.7
1,474,032 + Pf1N1B4_1292 0.83 -0.0
1,474,032 + Pf1N1B4_1292 0.83 +0.3
1,474,032 + Pf1N1B4_1292 0.83 -0.7
1,474,033 - Pf1N1B4_1292 0.84 -1.3
1,474,088 + +1.3
1,474,089 - Pf1N1B4_1292 0.88 -0.7
1,474,218 + -0.7
1,474,218 + -1.1
1,474,219 - +0.3
1,474,219 - +1.8
1,474,442 + Pf1N1B4_1293 0.21 +1.7
1,474,442 + Pf1N1B4_1293 0.21 +0.3
1,474,443 - Pf1N1B4_1293 0.21 +0.3
1,474,443 - Pf1N1B4_1293 0.21 -0.2
1,474,460 + -0.7
1,474,461 - Pf1N1B4_1293 0.24 +0.7
1,474,712 + Pf1N1B4_1293 0.55 +0.3
1,474,712 + +1.3
1,474,733 + Pf1N1B4_1293 0.58 +1.3
1,474,733 + Pf1N1B4_1293 0.58 +0.3
1,474,733 + Pf1N1B4_1293 0.58 +0.3
1,474,734 - Pf1N1B4_1293 0.58 -0.7
1,474,736 + Pf1N1B4_1293 0.58 -0.3
1,475,601 - Pf1N1B4_1294 0.51 -0.5
1,475,602 + Pf1N1B4_1294 0.51 -0.7
1,475,603 - Pf1N1B4_1294 0.51 +0.0
1,475,890 + Pf1N1B4_1294 0.78 +0.3
1,476,030 + -1.3
1,476,031 - +1.3
1,476,031 - -0.2

Or see this region's nucleotide sequence