Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1260 and Pf1N1B4_1261 are separated by 148 nucleotides Pf1N1B4_1261 and Pf1N1B4_1262 are separated by 191 nucleotides Pf1N1B4_1262 and Pf1N1B4_1263 overlap by 4 nucleotides Pf1N1B4_1263 and Pf1N1B4_1264 overlap by 47 nucleotides
Pf1N1B4_1260 - Copper chaperone, at 1,440,981 to 1,441,178
_1260
Pf1N1B4_1261 - FIG00953575: hypothetical protein, at 1,441,327 to 1,441,710
_1261
Pf1N1B4_1262 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 1,441,902 to 1,444,295
_1262
Pf1N1B4_1263 - Cu(I)-responsive transcriptional regulator, at 1,444,292 to 1,444,705
_1263
Pf1N1B4_1264 - Integral membrane efflux protein, at 1,444,659 to 1,445,873
_1264
Position (kb)
1441
1442
1443
1444
1445 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1440.970 kb on + strand at 1440.974 kb on - strand at 1441.000 kb on + strand at 1441.000 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.300 kb on + strand at 1441.301 kb on - strand at 1441.301 kb on - strand at 1441.301 kb on - strand at 1441.301 kb on - strand at 1441.301 kb on - strand at 1441.301 kb on - strand at 1441.367 kb on + strand, within Pf1N1B4_1261 at 1441.367 kb on + strand, within Pf1N1B4_1261 at 1441.367 kb on + strand, within Pf1N1B4_1261 at 1441.367 kb on + strand, within Pf1N1B4_1261 at 1441.367 kb on + strand, within Pf1N1B4_1261 at 1441.368 kb on - strand, within Pf1N1B4_1261 at 1441.408 kb on + strand at 1441.471 kb on + strand, within Pf1N1B4_1261 at 1441.472 kb on - strand, within Pf1N1B4_1261 at 1441.472 kb on - strand, within Pf1N1B4_1261 at 1441.472 kb on - strand, within Pf1N1B4_1261 at 1441.488 kb on - strand, within Pf1N1B4_1261 at 1441.681 kb on + strand at 1441.681 kb on + strand at 1441.681 kb on + strand at 1441.681 kb on + strand at 1441.681 kb on + strand at 1441.681 kb on + strand at 1441.682 kb on - strand at 1441.682 kb on - strand at 1441.682 kb on - strand at 1441.682 kb on - strand at 1441.682 kb on - strand at 1441.682 kb on - strand at 1441.682 kb on - strand at 1441.875 kb on - strand at 1441.875 kb on - strand at 1441.875 kb on - strand at 1441.878 kb on + strand at 1441.878 kb on + strand at 1441.886 kb on - strand at 1442.299 kb on - strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.514 kb on + strand, within Pf1N1B4_1262 at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.515 kb on - strand at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.515 kb on - strand, within Pf1N1B4_1262 at 1442.646 kb on + strand, within Pf1N1B4_1262 at 1442.646 kb on + strand, within Pf1N1B4_1262 at 1442.646 kb on + strand, within Pf1N1B4_1262 at 1442.646 kb on + strand, within Pf1N1B4_1262 at 1442.646 kb on + strand, within Pf1N1B4_1262 at 1442.665 kb on + strand, within Pf1N1B4_1262 at 1442.694 kb on + strand, within Pf1N1B4_1262 at 1442.694 kb on + strand, within Pf1N1B4_1262 at 1442.694 kb on + strand, within Pf1N1B4_1262 at 1442.695 kb on - strand, within Pf1N1B4_1262 at 1442.695 kb on - strand, within Pf1N1B4_1262 at 1442.695 kb on - strand, within Pf1N1B4_1262 at 1443.165 kb on + strand, within Pf1N1B4_1262 at 1443.785 kb on - strand, within Pf1N1B4_1262 at 1444.108 kb on - strand at 1444.146 kb on + strand at 1444.146 kb on + strand at 1444.146 kb on + strand at 1444.147 kb on - strand at 1444.147 kb on - strand at 1444.147 kb on - strand at 1444.249 kb on + strand at 1444.349 kb on + strand, within Pf1N1B4_1263 at 1444.349 kb on + strand, within Pf1N1B4_1263 at 1444.406 kb on + strand, within Pf1N1B4_1263 at 1444.426 kb on + strand, within Pf1N1B4_1263 at 1444.427 kb on - strand, within Pf1N1B4_1263 at 1444.618 kb on - strand, within Pf1N1B4_1263 at 1444.789 kb on + strand, within Pf1N1B4_1264 at 1444.790 kb on - strand, within Pf1N1B4_1264 at 1444.964 kb on - strand, within Pf1N1B4_1264 at 1445.055 kb on - strand, within Pf1N1B4_1264
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,440,970 + -1.3 1,440,974 - +0.3 1,441,000 + -1.1 1,441,000 + +1.8 1,441,300 + +1.3 1,441,300 + -1.7 1,441,300 + -0.7 1,441,300 + -0.3 1,441,300 + -0.2 1,441,300 + -0.2 1,441,300 + -0.9 1,441,300 + +1.5 1,441,300 + -0.7 1,441,301 - +1.0 1,441,301 - +2.6 1,441,301 - -1.6 1,441,301 - -0.7 1,441,301 - -0.7 1,441,301 - -0.6 1,441,367 + Pf1N1B4_1261 0.10 -0.1 1,441,367 + Pf1N1B4_1261 0.10 +0.3 1,441,367 + Pf1N1B4_1261 0.10 +0.8 1,441,367 + Pf1N1B4_1261 0.10 -2.1 1,441,367 + Pf1N1B4_1261 0.10 +0.3 1,441,368 - Pf1N1B4_1261 0.11 -0.6 1,441,408 + +0.3 1,441,471 + Pf1N1B4_1261 0.38 -0.6 1,441,472 - Pf1N1B4_1261 0.38 +0.1 1,441,472 - Pf1N1B4_1261 0.38 +0.3 1,441,472 - Pf1N1B4_1261 0.38 -0.7 1,441,488 - Pf1N1B4_1261 0.42 +1.3 1,441,681 + +0.8 1,441,681 + -0.2 1,441,681 + -1.3 1,441,681 + -0.2 1,441,681 + -0.5 1,441,681 + -1.0 1,441,682 - -0.6 1,441,682 - +0.8 1,441,682 - +1.8 1,441,682 - -0.2 1,441,682 - +1.3 1,441,682 - +0.5 1,441,682 - -0.7 1,441,875 - -1.7 1,441,875 - +0.3 1,441,875 - -0.0 1,441,878 + -1.6 1,441,878 + +1.1 1,441,886 - -2.1 1,442,299 - Pf1N1B4_1262 0.17 -1.3 1,442,514 + Pf1N1B4_1262 0.26 -1.1 1,442,514 + Pf1N1B4_1262 0.26 -0.3 1,442,514 + Pf1N1B4_1262 0.26 +0.2 1,442,514 + Pf1N1B4_1262 0.26 +1.8 1,442,514 + Pf1N1B4_1262 0.26 -2.1 1,442,514 + Pf1N1B4_1262 0.26 -2.3 1,442,514 + Pf1N1B4_1262 0.26 -1.6 1,442,514 + Pf1N1B4_1262 0.26 -1.6 1,442,514 + Pf1N1B4_1262 0.26 -0.8 1,442,514 + Pf1N1B4_1262 0.26 -1.1 1,442,514 + Pf1N1B4_1262 0.26 -1.3 1,442,515 - Pf1N1B4_1262 0.26 -0.8 1,442,515 - Pf1N1B4_1262 0.26 -0.1 1,442,515 - +0.2 1,442,515 - Pf1N1B4_1262 0.26 -0.0 1,442,515 - Pf1N1B4_1262 0.26 -0.3 1,442,515 - Pf1N1B4_1262 0.26 -0.9 1,442,515 - Pf1N1B4_1262 0.26 -0.4 1,442,515 - Pf1N1B4_1262 0.26 +2.2 1,442,646 + Pf1N1B4_1262 0.31 -1.8 1,442,646 + Pf1N1B4_1262 0.31 -1.3 1,442,646 + Pf1N1B4_1262 0.31 -2.1 1,442,646 + Pf1N1B4_1262 0.31 -0.8 1,442,646 + Pf1N1B4_1262 0.31 -1.8 1,442,665 + Pf1N1B4_1262 0.32 -1.3 1,442,694 + Pf1N1B4_1262 0.33 -0.8 1,442,694 + Pf1N1B4_1262 0.33 -1.8 1,442,694 + Pf1N1B4_1262 0.33 -2.3 1,442,695 - Pf1N1B4_1262 0.33 -2.3 1,442,695 - Pf1N1B4_1262 0.33 -0.3 1,442,695 - Pf1N1B4_1262 0.33 -2.6 1,443,165 + Pf1N1B4_1262 0.53 -1.9 1,443,785 - Pf1N1B4_1262 0.79 +1.8 1,444,108 - -2.1 1,444,146 + -0.8 1,444,146 + +0.2 1,444,146 + -0.3 1,444,147 - +0.2 1,444,147 - -0.8 1,444,147 - -0.3 1,444,249 + -2.3 1,444,349 + Pf1N1B4_1263 0.14 -0.8 1,444,349 + Pf1N1B4_1263 0.14 -1.8 1,444,406 + Pf1N1B4_1263 0.28 +0.2 1,444,426 + Pf1N1B4_1263 0.32 -1.3 1,444,427 - Pf1N1B4_1263 0.33 -1.1 1,444,618 - Pf1N1B4_1263 0.79 -1.1 1,444,789 + Pf1N1B4_1264 0.11 +0.8 1,444,790 - Pf1N1B4_1264 0.11 -1.1 1,444,964 - Pf1N1B4_1264 0.25 -0.9 1,445,055 - Pf1N1B4_1264 0.33 -1.1
Or see this region's nucleotide sequence