Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1228 and Pf1N1B4_1229 are separated by 10 nucleotides Pf1N1B4_1229 and Pf1N1B4_1230 are separated by 161 nucleotides
Pf1N1B4_1228 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 1,394,068 to 1,394,955
_1228
Pf1N1B4_1229 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 1,394,966 to 1,396,483
_1229
Pf1N1B4_1230 - Transcriptional regulator, LysR family, at 1,396,645 to 1,397,592
_1230
Position (kb)
1394
1395
1396
1397 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1393.978 kb on - strand at 1393.978 kb on - strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.145 kb on - strand at 1394.145 kb on - strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1395.051 kb on + strand at 1395.051 kb on + strand at 1395.193 kb on + strand, within Pf1N1B4_1229 at 1395.194 kb on - strand at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.610 kb on + strand, within Pf1N1B4_1229 at 1395.736 kb on + strand at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1396.024 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.156 kb on + strand, within Pf1N1B4_1229 at 1396.202 kb on + strand, within Pf1N1B4_1229 at 1396.203 kb on - strand, within Pf1N1B4_1229 at 1396.214 kb on - strand, within Pf1N1B4_1229 at 1396.484 kb on - strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.507 kb on - strand at 1396.507 kb on - strand at 1396.510 kb on + strand at 1396.510 kb on + strand at 1396.511 kb on - strand at 1396.511 kb on - strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.614 kb on + strand at 1396.615 kb on - strand at 1396.617 kb on + strand at 1396.629 kb on + strand at 1396.630 kb on - strand at 1396.667 kb on + strand at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.171 kb on - strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.285 kb on - strand, within Pf1N1B4_1230 at 1397.467 kb on + strand at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,393,978 - +1.2 1,393,978 - -2.8 1,394,144 + -2.0 1,394,144 + +0.2 1,394,144 + -1.2 1,394,144 + +0.2 1,394,145 - +1.2 1,394,145 - +2.2 1,394,303 + Pf1N1B4_1228 0.26 -1.8 1,394,303 + Pf1N1B4_1228 0.26 -0.1 1,394,303 + Pf1N1B4_1228 0.26 +0.2 1,394,303 + +0.2 1,394,303 + Pf1N1B4_1228 0.26 -0.8 1,394,304 - Pf1N1B4_1228 0.27 -2.1 1,394,304 - Pf1N1B4_1228 0.27 -0.8 1,394,304 - Pf1N1B4_1228 0.27 -1.9 1,394,304 - Pf1N1B4_1228 0.27 -0.8 1,394,304 - Pf1N1B4_1228 0.27 -2.1 1,394,304 - Pf1N1B4_1228 0.27 -1.8 1,394,304 - -1.1 1,394,304 - Pf1N1B4_1228 0.27 -1.8 1,394,304 - Pf1N1B4_1228 0.27 -2.9 1,395,051 + -0.5 1,395,051 + -2.1 1,395,193 + Pf1N1B4_1229 0.15 -2.4 1,395,194 - +0.3 1,395,327 + Pf1N1B4_1229 0.24 -1.1 1,395,327 + Pf1N1B4_1229 0.24 -1.3 1,395,327 + Pf1N1B4_1229 0.24 +0.3 1,395,327 + Pf1N1B4_1229 0.24 +0.7 1,395,327 + Pf1N1B4_1229 0.24 -2.1 1,395,327 + Pf1N1B4_1229 0.24 +0.3 1,395,327 + Pf1N1B4_1229 0.24 -0.7 1,395,328 - Pf1N1B4_1229 0.24 -0.1 1,395,328 - Pf1N1B4_1229 0.24 -0.7 1,395,610 + Pf1N1B4_1229 0.42 -1.5 1,395,736 + +0.3 1,395,760 - Pf1N1B4_1229 0.52 -0.7 1,395,760 - Pf1N1B4_1229 0.52 +0.3 1,396,024 + Pf1N1B4_1229 0.70 -1.8 1,396,059 + Pf1N1B4_1229 0.72 -0.3 1,396,059 + Pf1N1B4_1229 0.72 -0.3 1,396,059 + Pf1N1B4_1229 0.72 -2.3 1,396,059 + Pf1N1B4_1229 0.72 -2.1 1,396,059 + Pf1N1B4_1229 0.72 -2.3 1,396,059 + Pf1N1B4_1229 0.72 +0.8 1,396,059 + Pf1N1B4_1229 0.72 -1.0 1,396,059 + Pf1N1B4_1229 0.72 -1.6 1,396,060 - Pf1N1B4_1229 0.72 -1.2 1,396,060 - Pf1N1B4_1229 0.72 -0.9 1,396,060 - Pf1N1B4_1229 0.72 -1.2 1,396,156 + Pf1N1B4_1229 0.78 -1.3 1,396,202 + Pf1N1B4_1229 0.81 -0.2 1,396,203 - Pf1N1B4_1229 0.81 -0.7 1,396,214 - Pf1N1B4_1229 0.82 -1.5 1,396,484 - -2.6 1,396,506 + +1.0 1,396,506 + -0.7 1,396,506 + -1.9 1,396,506 + +0.3 1,396,506 + -1.3 1,396,507 - -0.3 1,396,507 - +0.6 1,396,510 + -0.7 1,396,510 + -1.5 1,396,511 - +1.3 1,396,511 - -1.7 1,396,598 + -0.7 1,396,598 + -0.7 1,396,598 + -2.3 1,396,598 + -0.7 1,396,598 + -2.3 1,396,598 + -1.7 1,396,598 + -0.3 1,396,598 + -1.1 1,396,599 - -0.2 1,396,599 - -1.7 1,396,599 - -0.7 1,396,599 - +0.3 1,396,599 - +1.3 1,396,599 - +1.3 1,396,599 - -1.3 1,396,599 - +1.4 1,396,599 - +1.8 1,396,599 - -1.3 1,396,614 + -2.3 1,396,615 - +1.8 1,396,617 + -1.9 1,396,629 + -1.3 1,396,630 - +0.5 1,396,667 + -1.7 1,396,759 + Pf1N1B4_1230 0.12 -2.7 1,396,759 + Pf1N1B4_1230 0.12 -0.7 1,396,759 + Pf1N1B4_1230 0.12 +0.3 1,396,760 - Pf1N1B4_1230 0.12 -0.1 1,396,760 - Pf1N1B4_1230 0.12 +0.9 1,396,760 - Pf1N1B4_1230 0.12 +0.3 1,397,131 + Pf1N1B4_1230 0.51 +0.3 1,397,131 + Pf1N1B4_1230 0.51 -1.7 1,397,131 + Pf1N1B4_1230 0.51 -0.7 1,397,132 - Pf1N1B4_1230 0.51 +1.8 1,397,132 - Pf1N1B4_1230 0.51 +0.6 1,397,170 + Pf1N1B4_1230 0.55 -1.3 1,397,170 + Pf1N1B4_1230 0.55 -1.3 1,397,170 + Pf1N1B4_1230 0.55 +0.3 1,397,170 + Pf1N1B4_1230 0.55 -1.3 1,397,171 - Pf1N1B4_1230 0.55 +0.3 1,397,248 + Pf1N1B4_1230 0.64 -1.7 1,397,248 + Pf1N1B4_1230 0.64 -1.7 1,397,248 + Pf1N1B4_1230 0.64 -1.3 1,397,248 + Pf1N1B4_1230 0.64 -3.2 1,397,248 + Pf1N1B4_1230 0.64 -1.3 1,397,249 - Pf1N1B4_1230 0.64 +1.3 1,397,249 - Pf1N1B4_1230 0.64 -0.3 1,397,249 - Pf1N1B4_1230 0.64 -1.1 1,397,249 - Pf1N1B4_1230 0.64 -1.1 1,397,284 + Pf1N1B4_1230 0.67 +0.3 1,397,284 + Pf1N1B4_1230 0.67 -1.1 1,397,285 - Pf1N1B4_1230 0.68 -1.3 1,397,467 + +0.3 1,397,468 - Pf1N1B4_1230 0.87 -2.1 1,397,468 - Pf1N1B4_1230 0.87 +0.8 1,397,468 - Pf1N1B4_1230 0.87 -0.7
Or see this region's nucleotide sequence