Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1153 and Pf1N1B4_1154 are separated by 83 nucleotides Pf1N1B4_1154 and Pf1N1B4_1155 are separated by 301 nucleotides Pf1N1B4_1155 and Pf1N1B4_5175 are separated by 11 nucleotides Pf1N1B4_5175 and Pf1N1B4_1156 are separated by 262 nucleotides
Pf1N1B4_1153 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 1,300,869 to 1,301,717
_1153
Pf1N1B4_1154 - FIG00957702: hypothetical protein, at 1,301,801 to 1,302,106
_1154
Pf1N1B4_1155 - hypothetical protein, at 1,302,408 to 1,302,953
_1155
Pf1N1B4_5175 - tRNA-Thr-CGT, at 1,302,965 to 1,303,037
_5175
Pf1N1B4_1156 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 1,303,300 to 1,305,405
_1156
Position (kb)
1302
1303 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1301.417 kb on + strand, within Pf1N1B4_1153 at 1301.615 kb on + strand, within Pf1N1B4_1153 at 1301.615 kb on + strand, within Pf1N1B4_1153 at 1301.616 kb on - strand, within Pf1N1B4_1153 at 1301.649 kb on + strand at 1301.649 kb on + strand at 1301.649 kb on + strand at 1301.651 kb on + strand at 1301.651 kb on + strand at 1301.652 kb on - strand at 1301.725 kb on + strand at 1301.725 kb on + strand at 1301.725 kb on + strand at 1301.732 kb on + strand at 1301.732 kb on + strand at 1301.737 kb on + strand at 1301.737 kb on + strand at 1301.737 kb on + strand at 1301.737 kb on + strand at 1301.738 kb on - strand at 1301.744 kb on + strand at 1301.744 kb on + strand at 1301.745 kb on - strand at 1301.767 kb on + strand at 1301.768 kb on - strand at 1301.811 kb on - strand at 1301.811 kb on - strand at 1301.811 kb on - strand at 1301.815 kb on + strand at 1301.816 kb on - strand at 1301.816 kb on - strand at 1301.816 kb on - strand at 1301.816 kb on - strand at 1301.829 kb on + strand at 1301.830 kb on - strand at 1301.852 kb on + strand, within Pf1N1B4_1154 at 1301.852 kb on + strand, within Pf1N1B4_1154 at 1301.853 kb on - strand, within Pf1N1B4_1154 at 1301.853 kb on - strand, within Pf1N1B4_1154 at 1301.853 kb on - strand, within Pf1N1B4_1154 at 1301.917 kb on - strand, within Pf1N1B4_1154 at 1301.917 kb on - strand, within Pf1N1B4_1154 at 1301.917 kb on - strand, within Pf1N1B4_1154 at 1301.917 kb on - strand, within Pf1N1B4_1154 at 1301.917 kb on - strand, within Pf1N1B4_1154 at 1302.052 kb on - strand, within Pf1N1B4_1154 at 1302.052 kb on - strand, within Pf1N1B4_1154 at 1302.052 kb on - strand at 1302.172 kb on + strand at 1302.172 kb on + strand at 1302.173 kb on - strand at 1302.173 kb on - strand at 1302.259 kb on + strand at 1302.260 kb on - strand at 1302.261 kb on + strand at 1302.261 kb on + strand at 1302.261 kb on + strand at 1302.261 kb on + strand at 1302.262 kb on - strand at 1302.262 kb on - strand at 1302.262 kb on - strand at 1302.262 kb on - strand at 1302.262 kb on - strand at 1302.262 kb on - strand at 1302.283 kb on - strand at 1302.283 kb on - strand at 1302.283 kb on - strand at 1302.294 kb on + strand at 1302.295 kb on - strand at 1302.295 kb on - strand at 1302.295 kb on - strand at 1302.295 kb on - strand at 1302.300 kb on + strand at 1302.300 kb on + strand at 1302.301 kb on - strand at 1302.301 kb on - strand at 1302.301 kb on - strand at 1302.308 kb on - strand at 1302.323 kb on + strand at 1302.323 kb on + strand at 1302.323 kb on + strand at 1302.323 kb on + strand at 1302.324 kb on - strand at 1302.324 kb on - strand at 1302.324 kb on - strand at 1302.348 kb on + strand at 1302.348 kb on + strand at 1302.348 kb on + strand at 1302.348 kb on + strand at 1302.348 kb on + strand at 1302.349 kb on - strand at 1302.349 kb on - strand at 1302.349 kb on - strand at 1302.349 kb on - strand at 1302.528 kb on + strand, within Pf1N1B4_1155 at 1302.817 kb on + strand, within Pf1N1B4_1155 at 1302.817 kb on + strand, within Pf1N1B4_1155 at 1302.818 kb on - strand, within Pf1N1B4_1155 at 1302.818 kb on - strand, within Pf1N1B4_1155 at 1302.818 kb on - strand, within Pf1N1B4_1155 at 1302.818 kb on - strand at 1302.818 kb on - strand, within Pf1N1B4_1155 at 1302.827 kb on + strand, within Pf1N1B4_1155 at 1302.827 kb on + strand, within Pf1N1B4_1155 at 1302.828 kb on - strand, within Pf1N1B4_1155 at 1302.951 kb on + strand at 1302.952 kb on - strand at 1303.029 kb on + strand, within Pf1N1B4_5175 at 1303.045 kb on + strand at 1303.052 kb on - strand at 1303.052 kb on - strand at 1303.121 kb on - strand at 1303.129 kb on - strand at 1303.148 kb on - strand at 1303.200 kb on + strand at 1303.201 kb on - strand at 1303.215 kb on + strand at 1303.215 kb on + strand at 1303.215 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,301,417 + Pf1N1B4_1153 0.65 -0.3 1,301,615 + Pf1N1B4_1153 0.88 -1.1 1,301,615 + Pf1N1B4_1153 0.88 -0.4 1,301,616 - Pf1N1B4_1153 0.88 -2.0 1,301,649 + -1.3 1,301,649 + -0.5 1,301,649 + -1.4 1,301,651 + +0.2 1,301,651 + -0.8 1,301,652 - -0.4 1,301,725 + +0.6 1,301,725 + -0.8 1,301,725 + +0.2 1,301,732 + -0.6 1,301,732 + -0.4 1,301,737 + -0.8 1,301,737 + +1.2 1,301,737 + +0.2 1,301,737 + -0.2 1,301,738 - -0.3 1,301,744 + -2.4 1,301,744 + +0.5 1,301,745 - -0.2 1,301,767 + +0.2 1,301,768 - +1.2 1,301,811 - -1.0 1,301,811 - +0.2 1,301,811 - +0.2 1,301,815 + +1.2 1,301,816 - +0.2 1,301,816 - -0.3 1,301,816 - +0.1 1,301,816 - -1.7 1,301,829 + -0.8 1,301,830 - -1.1 1,301,852 + Pf1N1B4_1154 0.17 +1.2 1,301,852 + Pf1N1B4_1154 0.17 -1.4 1,301,853 - Pf1N1B4_1154 0.17 +0.2 1,301,853 - Pf1N1B4_1154 0.17 +1.2 1,301,853 - Pf1N1B4_1154 0.17 -0.8 1,301,917 - Pf1N1B4_1154 0.38 +2.5 1,301,917 - Pf1N1B4_1154 0.38 +1.0 1,301,917 - Pf1N1B4_1154 0.38 -0.1 1,301,917 - Pf1N1B4_1154 0.38 -0.4 1,301,917 - Pf1N1B4_1154 0.38 -0.1 1,302,052 - Pf1N1B4_1154 0.82 -0.8 1,302,052 - Pf1N1B4_1154 0.82 -0.2 1,302,052 - +1.8 1,302,172 + +0.6 1,302,172 + +0.2 1,302,173 - +0.2 1,302,173 - +0.2 1,302,259 + -1.0 1,302,260 - +1.2 1,302,261 + -1.1 1,302,261 + +0.2 1,302,261 + +0.2 1,302,261 + +0.2 1,302,262 - -0.1 1,302,262 - -1.4 1,302,262 - +0.2 1,302,262 - +2.5 1,302,262 - -0.6 1,302,262 - +0.2 1,302,283 - -0.4 1,302,283 - -1.4 1,302,283 - -0.2 1,302,294 + -1.4 1,302,295 - +0.2 1,302,295 - +1.2 1,302,295 - +0.2 1,302,295 - +0.8 1,302,300 + -0.4 1,302,300 + -0.4 1,302,301 - +1.8 1,302,301 - +0.9 1,302,301 - +1.8 1,302,308 - +0.6 1,302,323 + +0.8 1,302,323 + +1.2 1,302,323 + +0.3 1,302,323 + +0.2 1,302,324 - -1.8 1,302,324 - -0.4 1,302,324 - -0.7 1,302,348 + +0.8 1,302,348 + -1.4 1,302,348 + +2.5 1,302,348 + -2.1 1,302,348 + -1.1 1,302,349 - +0.8 1,302,349 - -1.7 1,302,349 - -1.4 1,302,349 - -0.0 1,302,528 + Pf1N1B4_1155 0.22 +1.2 1,302,817 + Pf1N1B4_1155 0.75 +0.6 1,302,817 + Pf1N1B4_1155 0.75 +0.8 1,302,818 - Pf1N1B4_1155 0.75 -0.2 1,302,818 - Pf1N1B4_1155 0.75 -0.5 1,302,818 - Pf1N1B4_1155 0.75 +0.2 1,302,818 - -1.4 1,302,818 - Pf1N1B4_1155 0.75 +0.9 1,302,827 + Pf1N1B4_1155 0.77 +0.3 1,302,827 + Pf1N1B4_1155 0.77 +1.2 1,302,828 - Pf1N1B4_1155 0.77 -0.4 1,302,951 + +0.6 1,302,952 - +0.1 1,303,029 + Pf1N1B4_5175 0.88 -1.1 1,303,045 + +0.1 1,303,052 - +0.2 1,303,052 - +0.2 1,303,121 - -0.8 1,303,129 - +0.2 1,303,148 - +1.8 1,303,200 + +0.2 1,303,201 - +0.2 1,303,215 + -0.2 1,303,215 + +0.7 1,303,215 + -0.4
Or see this region's nucleotide sequence