Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1138 and Pf1N1B4_1139 are separated by 335 nucleotides Pf1N1B4_1139 and Pf1N1B4_1140 are separated by 0 nucleotides Pf1N1B4_1140 and Pf1N1B4_1141 are separated by 206 nucleotides
Pf1N1B4_1138 - Complete genome; segment 11/17, at 1,275,679 to 1,277,805
_1138
Pf1N1B4_1139 - FOG: TPR repeat, SEL1 subfamily, at 1,278,141 to 1,278,917
_1139
Pf1N1B4_1140 - Iron-uptake factor PiuC, at 1,278,918 to 1,279,598
_1140
Pf1N1B4_1141 - Ferrichrome-iron receptor, at 1,279,805 to 1,282,063
_1141
Position (kb)
1278
1279 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1277.329 kb on - strand, within Pf1N1B4_1138 at 1277.513 kb on - strand, within Pf1N1B4_1138 at 1277.523 kb on - strand, within Pf1N1B4_1138 at 1277.546 kb on + strand, within Pf1N1B4_1138 at 1277.547 kb on - strand, within Pf1N1B4_1138 at 1277.554 kb on + strand, within Pf1N1B4_1138 at 1277.555 kb on - strand, within Pf1N1B4_1138 at 1277.555 kb on - strand, within Pf1N1B4_1138 at 1277.555 kb on - strand, within Pf1N1B4_1138 at 1277.737 kb on - strand at 1277.739 kb on - strand at 1277.943 kb on + strand at 1278.089 kb on + strand at 1278.300 kb on + strand, within Pf1N1B4_1139 at 1278.339 kb on - strand, within Pf1N1B4_1139 at 1278.347 kb on - strand, within Pf1N1B4_1139 at 1278.446 kb on - strand, within Pf1N1B4_1139 at 1278.562 kb on + strand at 1278.562 kb on + strand, within Pf1N1B4_1139 at 1278.569 kb on - strand, within Pf1N1B4_1139 at 1278.569 kb on - strand, within Pf1N1B4_1139 at 1278.569 kb on - strand, within Pf1N1B4_1139 at 1278.569 kb on - strand, within Pf1N1B4_1139 at 1278.569 kb on - strand, within Pf1N1B4_1139 at 1278.569 kb on - strand, within Pf1N1B4_1139 at 1278.901 kb on - strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.919 kb on + strand at 1278.920 kb on - strand at 1278.920 kb on - strand at 1278.920 kb on - strand at 1278.920 kb on - strand at 1278.952 kb on + strand at 1278.952 kb on + strand at 1278.952 kb on + strand at 1278.952 kb on + strand at 1278.953 kb on - strand at 1278.953 kb on - strand at 1278.953 kb on - strand at 1278.953 kb on - strand at 1278.953 kb on - strand at 1278.953 kb on - strand at 1279.059 kb on + strand, within Pf1N1B4_1140 at 1279.060 kb on - strand, within Pf1N1B4_1140 at 1279.060 kb on - strand, within Pf1N1B4_1140 at 1279.219 kb on + strand, within Pf1N1B4_1140 at 1279.219 kb on + strand, within Pf1N1B4_1140 at 1279.220 kb on - strand, within Pf1N1B4_1140 at 1279.220 kb on - strand, within Pf1N1B4_1140 at 1279.339 kb on + strand, within Pf1N1B4_1140 at 1279.340 kb on - strand, within Pf1N1B4_1140 at 1279.340 kb on - strand, within Pf1N1B4_1140 at 1279.695 kb on + strand at 1279.695 kb on + strand at 1279.695 kb on + strand at 1279.696 kb on - strand at 1279.696 kb on - strand at 1279.696 kb on - strand at 1279.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,277,329 - Pf1N1B4_1138 0.78 +1.2 1,277,513 - Pf1N1B4_1138 0.86 +0.8 1,277,523 - Pf1N1B4_1138 0.87 +0.4 1,277,546 + Pf1N1B4_1138 0.88 -0.0 1,277,547 - Pf1N1B4_1138 0.88 +1.2 1,277,554 + Pf1N1B4_1138 0.88 -0.6 1,277,555 - Pf1N1B4_1138 0.88 -0.8 1,277,555 - Pf1N1B4_1138 0.88 -0.4 1,277,555 - Pf1N1B4_1138 0.88 -0.1 1,277,737 - +2.2 1,277,739 - +0.3 1,277,943 + +1.8 1,278,089 + -1.4 1,278,300 + Pf1N1B4_1139 0.20 +2.5 1,278,339 - Pf1N1B4_1139 0.25 -0.8 1,278,347 - Pf1N1B4_1139 0.27 +0.2 1,278,446 - Pf1N1B4_1139 0.39 +1.2 1,278,562 + -1.1 1,278,562 + Pf1N1B4_1139 0.54 +0.7 1,278,569 - Pf1N1B4_1139 0.55 +0.2 1,278,569 - Pf1N1B4_1139 0.55 +0.6 1,278,569 - Pf1N1B4_1139 0.55 +0.2 1,278,569 - Pf1N1B4_1139 0.55 +1.2 1,278,569 - Pf1N1B4_1139 0.55 -1.4 1,278,569 - Pf1N1B4_1139 0.55 -0.4 1,278,901 - +0.8 1,278,919 + -0.6 1,278,919 + +0.8 1,278,919 + +0.2 1,278,919 + -0.2 1,278,919 + +0.4 1,278,919 + +0.2 1,278,919 + -0.9 1,278,919 + +0.2 1,278,919 + +1.5 1,278,919 + +0.6 1,278,919 + +0.8 1,278,919 + +0.6 1,278,920 - -0.8 1,278,920 - +0.2 1,278,920 - -0.6 1,278,920 - -1.4 1,278,952 + +0.8 1,278,952 + -0.8 1,278,952 + +0.2 1,278,952 + +1.2 1,278,953 - +0.2 1,278,953 - -1.1 1,278,953 - +0.8 1,278,953 - -0.1 1,278,953 - +1.2 1,278,953 - -2.1 1,279,059 + Pf1N1B4_1140 0.21 +0.6 1,279,060 - Pf1N1B4_1140 0.21 +0.8 1,279,060 - Pf1N1B4_1140 0.21 +0.2 1,279,219 + Pf1N1B4_1140 0.44 -0.8 1,279,219 + Pf1N1B4_1140 0.44 +0.9 1,279,220 - Pf1N1B4_1140 0.44 -1.4 1,279,220 - Pf1N1B4_1140 0.44 -2.4 1,279,339 + Pf1N1B4_1140 0.62 -0.2 1,279,340 - Pf1N1B4_1140 0.62 +0.4 1,279,340 - Pf1N1B4_1140 0.62 -0.4 1,279,695 + -0.6 1,279,695 + -0.1 1,279,695 + -0.8 1,279,696 - +1.8 1,279,696 - -0.8 1,279,696 - -0.8 1,279,696 - -1.3
Or see this region's nucleotide sequence