Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1123 and Pf1N1B4_1124 are separated by 73 nucleotides Pf1N1B4_1124 and Pf1N1B4_1125 are separated by 10 nucleotides Pf1N1B4_1125 and Pf1N1B4_1126 are separated by 10 nucleotides
Pf1N1B4_1123 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 1,261,170 to 1,262,738
_1123
Pf1N1B4_1124 - Dipeptide transport system permease protein DppB (TC 3.A.1.5.2), at 1,262,812 to 1,263,822
_1124
Pf1N1B4_1125 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 1,263,833 to 1,264,744
_1125
Pf1N1B4_1126 - Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2), at 1,264,755 to 1,265,723
_1126
Position (kb)
1262
1263
1264 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1261.821 kb on + strand, within Pf1N1B4_1123 at 1261.821 kb on + strand, within Pf1N1B4_1123 at 1261.822 kb on - strand, within Pf1N1B4_1123 at 1261.822 kb on - strand, within Pf1N1B4_1123 at 1261.927 kb on - strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.007 kb on + strand, within Pf1N1B4_1123 at 1262.008 kb on - strand, within Pf1N1B4_1123 at 1262.008 kb on - strand, within Pf1N1B4_1123 at 1262.008 kb on - strand, within Pf1N1B4_1123 at 1262.008 kb on - strand at 1262.034 kb on + strand, within Pf1N1B4_1123 at 1262.034 kb on + strand, within Pf1N1B4_1123 at 1262.034 kb on + strand, within Pf1N1B4_1123 at 1262.034 kb on + strand, within Pf1N1B4_1123 at 1262.034 kb on + strand, within Pf1N1B4_1123 at 1262.035 kb on - strand, within Pf1N1B4_1123 at 1262.035 kb on - strand, within Pf1N1B4_1123 at 1262.058 kb on - strand, within Pf1N1B4_1123 at 1262.091 kb on + strand, within Pf1N1B4_1123 at 1262.091 kb on + strand, within Pf1N1B4_1123 at 1262.091 kb on + strand, within Pf1N1B4_1123 at 1262.091 kb on + strand, within Pf1N1B4_1123 at 1262.092 kb on - strand, within Pf1N1B4_1123 at 1262.163 kb on + strand, within Pf1N1B4_1123 at 1262.163 kb on + strand, within Pf1N1B4_1123 at 1262.163 kb on + strand, within Pf1N1B4_1123 at 1262.164 kb on - strand, within Pf1N1B4_1123 at 1262.252 kb on + strand, within Pf1N1B4_1123 at 1262.354 kb on + strand, within Pf1N1B4_1123 at 1262.505 kb on + strand, within Pf1N1B4_1123 at 1262.558 kb on + strand, within Pf1N1B4_1123 at 1262.589 kb on + strand at 1262.590 kb on - strand at 1262.651 kb on + strand at 1262.651 kb on + strand at 1262.651 kb on + strand at 1262.651 kb on + strand at 1262.677 kb on + strand at 1262.677 kb on + strand at 1262.677 kb on + strand at 1262.677 kb on + strand at 1262.677 kb on + strand at 1262.678 kb on - strand at 1262.711 kb on + strand at 1262.711 kb on + strand at 1262.711 kb on + strand at 1262.711 kb on + strand at 1262.711 kb on + strand at 1262.736 kb on + strand at 1262.736 kb on + strand at 1262.737 kb on - strand at 1262.737 kb on - strand at 1262.737 kb on - strand at 1262.737 kb on - strand at 1262.740 kb on + strand at 1262.740 kb on + strand at 1262.741 kb on - strand at 1262.802 kb on - strand at 1262.802 kb on - strand at 1262.817 kb on + strand at 1262.817 kb on + strand at 1262.817 kb on + strand at 1262.818 kb on - strand at 1262.818 kb on - strand at 1262.823 kb on + strand at 1262.843 kb on + strand at 1262.921 kb on + strand, within Pf1N1B4_1124 at 1262.921 kb on + strand, within Pf1N1B4_1124 at 1262.921 kb on + strand at 1262.921 kb on + strand, within Pf1N1B4_1124 at 1262.921 kb on + strand, within Pf1N1B4_1124 at 1262.922 kb on - strand, within Pf1N1B4_1124 at 1262.922 kb on - strand, within Pf1N1B4_1124 at 1262.922 kb on - strand, within Pf1N1B4_1124 at 1262.922 kb on - strand, within Pf1N1B4_1124 at 1262.978 kb on + strand, within Pf1N1B4_1124 at 1262.979 kb on - strand, within Pf1N1B4_1124 at 1262.998 kb on + strand, within Pf1N1B4_1124 at 1263.016 kb on + strand, within Pf1N1B4_1124 at 1263.016 kb on + strand, within Pf1N1B4_1124 at 1263.016 kb on + strand, within Pf1N1B4_1124 at 1263.017 kb on - strand, within Pf1N1B4_1124 at 1263.017 kb on - strand, within Pf1N1B4_1124 at 1263.017 kb on - strand, within Pf1N1B4_1124 at 1263.017 kb on - strand, within Pf1N1B4_1124 at 1263.017 kb on - strand, within Pf1N1B4_1124 at 1263.095 kb on + strand at 1263.096 kb on - strand, within Pf1N1B4_1124 at 1263.096 kb on - strand, within Pf1N1B4_1124 at 1263.135 kb on - strand, within Pf1N1B4_1124 at 1263.328 kb on + strand, within Pf1N1B4_1124 at 1263.328 kb on + strand, within Pf1N1B4_1124 at 1263.367 kb on - strand, within Pf1N1B4_1124 at 1263.508 kb on + strand, within Pf1N1B4_1124 at 1263.509 kb on - strand, within Pf1N1B4_1124 at 1263.509 kb on - strand, within Pf1N1B4_1124 at 1263.509 kb on - strand, within Pf1N1B4_1124 at 1263.785 kb on - strand at 1263.848 kb on - strand at 1263.848 kb on - strand at 1263.879 kb on - strand at 1263.891 kb on - strand at 1263.891 kb on - strand at 1263.893 kb on - strand at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.410 kb on - strand, within Pf1N1B4_1125 at 1264.566 kb on + strand, within Pf1N1B4_1125 at 1264.566 kb on + strand, within Pf1N1B4_1125 at 1264.567 kb on - strand, within Pf1N1B4_1125 at 1264.567 kb on - strand, within Pf1N1B4_1125 at 1264.617 kb on - strand, within Pf1N1B4_1125 at 1264.617 kb on - strand, within Pf1N1B4_1125 at 1264.766 kb on - strand at 1264.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,261,821 + Pf1N1B4_1123 0.41 +0.7 1,261,821 + Pf1N1B4_1123 0.41 +0.2 1,261,822 - Pf1N1B4_1123 0.42 +0.2 1,261,822 - Pf1N1B4_1123 0.42 -1.8 1,261,927 - Pf1N1B4_1123 0.48 +0.2 1,262,007 + Pf1N1B4_1123 0.53 -0.8 1,262,007 + Pf1N1B4_1123 0.53 +0.0 1,262,007 + Pf1N1B4_1123 0.53 -0.8 1,262,007 + Pf1N1B4_1123 0.53 -1.1 1,262,007 + Pf1N1B4_1123 0.53 +1.4 1,262,007 + Pf1N1B4_1123 0.53 +1.7 1,262,007 + Pf1N1B4_1123 0.53 -0.3 1,262,007 + Pf1N1B4_1123 0.53 +0.6 1,262,007 + Pf1N1B4_1123 0.53 +0.6 1,262,007 + Pf1N1B4_1123 0.53 -0.8 1,262,008 - Pf1N1B4_1123 0.53 -0.3 1,262,008 - Pf1N1B4_1123 0.53 -0.4 1,262,008 - Pf1N1B4_1123 0.53 -1.4 1,262,008 - -0.8 1,262,034 + Pf1N1B4_1123 0.55 -0.8 1,262,034 + Pf1N1B4_1123 0.55 -0.8 1,262,034 + Pf1N1B4_1123 0.55 -1.7 1,262,034 + Pf1N1B4_1123 0.55 -0.4 1,262,034 + Pf1N1B4_1123 0.55 -1.4 1,262,035 - Pf1N1B4_1123 0.55 +1.5 1,262,035 - Pf1N1B4_1123 0.55 +0.2 1,262,058 - Pf1N1B4_1123 0.57 +0.2 1,262,091 + Pf1N1B4_1123 0.59 +0.2 1,262,091 + Pf1N1B4_1123 0.59 -0.5 1,262,091 + Pf1N1B4_1123 0.59 +0.2 1,262,091 + Pf1N1B4_1123 0.59 -0.4 1,262,092 - Pf1N1B4_1123 0.59 +0.5 1,262,163 + Pf1N1B4_1123 0.63 -0.4 1,262,163 + Pf1N1B4_1123 0.63 +0.9 1,262,163 + Pf1N1B4_1123 0.63 -0.8 1,262,164 - Pf1N1B4_1123 0.63 +2.7 1,262,252 + Pf1N1B4_1123 0.69 -0.5 1,262,354 + Pf1N1B4_1123 0.75 -0.3 1,262,505 + Pf1N1B4_1123 0.85 +0.1 1,262,558 + Pf1N1B4_1123 0.88 -1.0 1,262,589 + -0.7 1,262,590 - -0.1 1,262,651 + +0.3 1,262,651 + +0.2 1,262,651 + +0.2 1,262,651 + +0.2 1,262,677 + +0.2 1,262,677 + -1.8 1,262,677 + +0.2 1,262,677 + +0.2 1,262,677 + +0.6 1,262,678 - +1.2 1,262,711 + -1.1 1,262,711 + +0.4 1,262,711 + +0.2 1,262,711 + -0.7 1,262,711 + +0.2 1,262,736 + +0.2 1,262,736 + -1.6 1,262,737 - -0.8 1,262,737 - +0.7 1,262,737 - +0.3 1,262,737 - -1.1 1,262,740 + +0.2 1,262,740 + -1.1 1,262,741 - +1.2 1,262,802 - -0.3 1,262,802 - -1.3 1,262,817 + -0.4 1,262,817 + -0.7 1,262,817 + -0.6 1,262,818 - +1.7 1,262,818 - +1.3 1,262,823 + -1.2 1,262,843 + +0.2 1,262,921 + Pf1N1B4_1124 0.11 +0.2 1,262,921 + Pf1N1B4_1124 0.11 -1.4 1,262,921 + +1.2 1,262,921 + Pf1N1B4_1124 0.11 +0.2 1,262,921 + Pf1N1B4_1124 0.11 +0.2 1,262,922 - Pf1N1B4_1124 0.11 +0.2 1,262,922 - Pf1N1B4_1124 0.11 +1.7 1,262,922 - Pf1N1B4_1124 0.11 +0.4 1,262,922 - Pf1N1B4_1124 0.11 -1.2 1,262,978 + Pf1N1B4_1124 0.16 +0.5 1,262,979 - Pf1N1B4_1124 0.17 +2.2 1,262,998 + Pf1N1B4_1124 0.18 -0.8 1,263,016 + Pf1N1B4_1124 0.20 +0.6 1,263,016 + Pf1N1B4_1124 0.20 -1.4 1,263,016 + Pf1N1B4_1124 0.20 +0.7 1,263,017 - Pf1N1B4_1124 0.20 -0.8 1,263,017 - Pf1N1B4_1124 0.20 +1.7 1,263,017 - Pf1N1B4_1124 0.20 +0.7 1,263,017 - Pf1N1B4_1124 0.20 -1.6 1,263,017 - Pf1N1B4_1124 0.20 +0.2 1,263,095 + +2.2 1,263,096 - Pf1N1B4_1124 0.28 +2.5 1,263,096 - Pf1N1B4_1124 0.28 -0.6 1,263,135 - Pf1N1B4_1124 0.32 +1.2 1,263,328 + Pf1N1B4_1124 0.51 -1.8 1,263,328 + Pf1N1B4_1124 0.51 +0.2 1,263,367 - Pf1N1B4_1124 0.55 -0.4 1,263,508 + Pf1N1B4_1124 0.69 +1.7 1,263,509 - Pf1N1B4_1124 0.69 -1.8 1,263,509 - Pf1N1B4_1124 0.69 -0.3 1,263,509 - Pf1N1B4_1124 0.69 +1.7 1,263,785 - +0.2 1,263,848 - -0.1 1,263,848 - -0.2 1,263,879 - +1.7 1,263,891 - +0.2 1,263,891 - -0.4 1,263,893 - +0.7 1,264,361 + Pf1N1B4_1125 0.58 +0.9 1,264,361 + Pf1N1B4_1125 0.58 -0.5 1,264,361 + Pf1N1B4_1125 0.58 -1.2 1,264,361 + Pf1N1B4_1125 0.58 -1.0 1,264,361 + Pf1N1B4_1125 0.58 -1.4 1,264,361 + Pf1N1B4_1125 0.58 -0.8 1,264,361 + Pf1N1B4_1125 0.58 -0.8 1,264,362 - Pf1N1B4_1125 0.58 -1.5 1,264,362 - Pf1N1B4_1125 0.58 +0.6 1,264,362 - Pf1N1B4_1125 0.58 +1.2 1,264,362 - Pf1N1B4_1125 0.58 -1.8 1,264,362 - Pf1N1B4_1125 0.58 -2.2 1,264,362 - Pf1N1B4_1125 0.58 +1.2 1,264,362 - Pf1N1B4_1125 0.58 -0.4 1,264,410 - Pf1N1B4_1125 0.63 +0.2 1,264,566 + Pf1N1B4_1125 0.80 -1.3 1,264,566 + Pf1N1B4_1125 0.80 -0.7 1,264,567 - Pf1N1B4_1125 0.80 -2.4 1,264,567 - Pf1N1B4_1125 0.80 -0.8 1,264,617 - Pf1N1B4_1125 0.86 -0.3 1,264,617 - Pf1N1B4_1125 0.86 +0.4 1,264,766 - -0.4 1,264,766 - -0.4
Or see this region's nucleotide sequence