Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1111 and Pf1N1B4_1112 are separated by 14 nucleotides Pf1N1B4_1112 and Pf1N1B4_1113 are separated by 139 nucleotides Pf1N1B4_1113 and Pf1N1B4_1114 are separated by 63 nucleotides
Pf1N1B4_1111 - Phosphatidylcholine-hydrolyzing phospholipase C (EC 3.1.4.3), at 1,245,644 to 1,246,963
_1111
Pf1N1B4_1112 - hypothetical protein, at 1,246,978 to 1,247,169
_1112
Pf1N1B4_1113 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 1,247,309 to 1,248,184
_1113
Pf1N1B4_1114 - ABC transporter, ATP-binding protein, at 1,248,248 to 1,249,975
_1114
Position (kb)
1247
1248
1249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.338 kb on - strand at 1246.418 kb on + strand, within Pf1N1B4_1111 at 1246.419 kb on - strand, within Pf1N1B4_1111 at 1246.458 kb on + strand, within Pf1N1B4_1111 at 1246.459 kb on - strand, within Pf1N1B4_1111 at 1246.459 kb on - strand, within Pf1N1B4_1111 at 1246.459 kb on - strand, within Pf1N1B4_1111 at 1246.628 kb on + strand, within Pf1N1B4_1111 at 1246.629 kb on - strand, within Pf1N1B4_1111 at 1246.709 kb on + strand, within Pf1N1B4_1111 at 1246.709 kb on + strand, within Pf1N1B4_1111 at 1246.709 kb on + strand, within Pf1N1B4_1111 at 1246.710 kb on - strand, within Pf1N1B4_1111 at 1246.710 kb on - strand, within Pf1N1B4_1111 at 1246.779 kb on + strand, within Pf1N1B4_1111 at 1246.779 kb on + strand, within Pf1N1B4_1111 at 1246.779 kb on + strand, within Pf1N1B4_1111 at 1246.904 kb on + strand at 1246.904 kb on + strand at 1246.905 kb on - strand at 1246.905 kb on - strand at 1246.905 kb on - strand at 1246.922 kb on + strand at 1246.922 kb on + strand at 1246.923 kb on - strand at 1246.983 kb on + strand at 1246.983 kb on + strand at 1246.984 kb on - strand at 1247.175 kb on + strand at 1247.175 kb on + strand at 1247.175 kb on + strand at 1247.175 kb on + strand at 1247.176 kb on - strand at 1247.176 kb on - strand at 1247.231 kb on + strand at 1247.242 kb on + strand at 1247.242 kb on + strand at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.463 kb on + strand, within Pf1N1B4_1113 at 1247.463 kb on + strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.557 kb on - strand, within Pf1N1B4_1113 at 1247.557 kb on - strand, within Pf1N1B4_1113 at 1248.099 kb on + strand at 1248.099 kb on + strand at 1248.099 kb on + strand at 1248.100 kb on - strand at 1248.100 kb on - strand at 1248.115 kb on + strand at 1248.116 kb on - strand at 1248.116 kb on - strand at 1248.432 kb on + strand, within Pf1N1B4_1114 at 1248.433 kb on - strand, within Pf1N1B4_1114 at 1248.433 kb on - strand, within Pf1N1B4_1114 at 1248.433 kb on - strand, within Pf1N1B4_1114 at 1248.450 kb on + strand, within Pf1N1B4_1114 at 1248.451 kb on - strand, within Pf1N1B4_1114 at 1248.577 kb on - strand, within Pf1N1B4_1114 at 1248.634 kb on + strand, within Pf1N1B4_1114
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,246,337 + Pf1N1B4_1111 0.53 +1.7 1,246,337 + Pf1N1B4_1111 0.53 +0.4 1,246,337 + Pf1N1B4_1111 0.53 -0.7 1,246,337 + Pf1N1B4_1111 0.53 -1.2 1,246,337 + Pf1N1B4_1111 0.53 -0.2 1,246,338 - -1.4 1,246,418 + Pf1N1B4_1111 0.59 +0.2 1,246,419 - Pf1N1B4_1111 0.59 -1.2 1,246,458 + Pf1N1B4_1111 0.62 -1.4 1,246,459 - Pf1N1B4_1111 0.62 -0.5 1,246,459 - Pf1N1B4_1111 0.62 -0.4 1,246,459 - Pf1N1B4_1111 0.62 +0.2 1,246,628 + Pf1N1B4_1111 0.75 +2.0 1,246,629 - Pf1N1B4_1111 0.75 -3.4 1,246,709 + Pf1N1B4_1111 0.81 +0.2 1,246,709 + Pf1N1B4_1111 0.81 +1.5 1,246,709 + Pf1N1B4_1111 0.81 -1.2 1,246,710 - Pf1N1B4_1111 0.81 -0.8 1,246,710 - Pf1N1B4_1111 0.81 +0.7 1,246,779 + Pf1N1B4_1111 0.86 -0.4 1,246,779 + Pf1N1B4_1111 0.86 +0.2 1,246,779 + Pf1N1B4_1111 0.86 -1.1 1,246,904 + -0.4 1,246,904 + -0.3 1,246,905 - +0.9 1,246,905 - -0.2 1,246,905 - +0.7 1,246,922 + +0.2 1,246,922 + +0.2 1,246,923 - +1.2 1,246,983 + +0.2 1,246,983 + -0.4 1,246,984 - +0.7 1,247,175 + +0.2 1,247,175 + -1.4 1,247,175 + +0.2 1,247,175 + -0.8 1,247,176 - +2.5 1,247,176 - +0.2 1,247,231 + +1.2 1,247,242 + -1.8 1,247,242 + -0.1 1,247,428 - Pf1N1B4_1113 0.14 -0.4 1,247,428 - Pf1N1B4_1113 0.14 -1.8 1,247,428 - Pf1N1B4_1113 0.14 -0.8 1,247,428 - Pf1N1B4_1113 0.14 -2.4 1,247,463 + Pf1N1B4_1113 0.18 -2.3 1,247,463 + Pf1N1B4_1113 0.18 -1.4 1,247,464 - Pf1N1B4_1113 0.18 -2.2 1,247,464 - Pf1N1B4_1113 0.18 -1.0 1,247,464 - Pf1N1B4_1113 0.18 -1.4 1,247,464 - Pf1N1B4_1113 0.18 -2.2 1,247,464 - Pf1N1B4_1113 0.18 -0.3 1,247,464 - Pf1N1B4_1113 0.18 -1.2 1,247,464 - Pf1N1B4_1113 0.18 +3.3 1,247,464 - Pf1N1B4_1113 0.18 -1.4 1,247,464 - Pf1N1B4_1113 0.18 -1.4 1,247,464 - Pf1N1B4_1113 0.18 -2.2 1,247,464 - Pf1N1B4_1113 0.18 -1.4 1,247,464 - Pf1N1B4_1113 0.18 -0.4 1,247,557 - Pf1N1B4_1113 0.28 +0.7 1,247,557 - Pf1N1B4_1113 0.28 -1.3 1,248,099 + -0.4 1,248,099 + -0.8 1,248,099 + -1.8 1,248,100 - -0.6 1,248,100 - -0.8 1,248,115 + -1.2 1,248,116 - -0.2 1,248,116 - -2.2 1,248,432 + Pf1N1B4_1114 0.11 +0.2 1,248,433 - Pf1N1B4_1114 0.11 -0.2 1,248,433 - Pf1N1B4_1114 0.11 +0.9 1,248,433 - Pf1N1B4_1114 0.11 +0.6 1,248,450 + Pf1N1B4_1114 0.12 +0.2 1,248,451 - Pf1N1B4_1114 0.12 -1.8 1,248,577 - Pf1N1B4_1114 0.19 +0.9 1,248,634 + Pf1N1B4_1114 0.22 +1.2
Or see this region's nucleotide sequence