Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1033 and Pf1N1B4_1034 overlap by 30 nucleotides Pf1N1B4_1034 and Pf1N1B4_1035 overlap by 20 nucleotides Pf1N1B4_1035 and Pf1N1B4_1036 are separated by 103 nucleotides Pf1N1B4_1036 and Pf1N1B4_1037 are separated by 54 nucleotides
Pf1N1B4_1033 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 1,174,260 to 1,175,042
_1033
Pf1N1B4_1034 - hypothetical protein, at 1,175,013 to 1,175,279
_1034
Pf1N1B4_1035 - Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C), at 1,175,260 to 1,175,715
_1035
Pf1N1B4_1036 - Signal transduction histidine kinase, at 1,175,819 to 1,176,295
_1036
Pf1N1B4_1037 - FIG00953110: hypothetical protein, at 1,176,350 to 1,176,955
_1037
Position (kb)
1175
1176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1174.261 kb on + strand at 1174.268 kb on + strand at 1174.268 kb on + strand at 1174.269 kb on - strand at 1174.269 kb on - strand at 1174.269 kb on - strand at 1174.432 kb on - strand, within Pf1N1B4_1033 at 1174.460 kb on + strand, within Pf1N1B4_1033 at 1174.460 kb on + strand, within Pf1N1B4_1033 at 1174.460 kb on + strand, within Pf1N1B4_1033 at 1174.460 kb on + strand, within Pf1N1B4_1033 at 1174.461 kb on - strand, within Pf1N1B4_1033 at 1174.461 kb on - strand, within Pf1N1B4_1033 at 1174.461 kb on - strand, within Pf1N1B4_1033 at 1174.461 kb on - strand, within Pf1N1B4_1033 at 1174.597 kb on + strand, within Pf1N1B4_1033 at 1174.598 kb on - strand, within Pf1N1B4_1033 at 1174.598 kb on - strand, within Pf1N1B4_1033 at 1174.606 kb on + strand, within Pf1N1B4_1033 at 1174.606 kb on + strand, within Pf1N1B4_1033 at 1174.607 kb on - strand, within Pf1N1B4_1033 at 1174.707 kb on + strand, within Pf1N1B4_1033 at 1174.708 kb on - strand, within Pf1N1B4_1033 at 1174.708 kb on - strand, within Pf1N1B4_1033 at 1174.708 kb on - strand, within Pf1N1B4_1033 at 1174.708 kb on - strand, within Pf1N1B4_1033 at 1174.756 kb on + strand, within Pf1N1B4_1033 at 1174.778 kb on - strand, within Pf1N1B4_1033 at 1174.778 kb on - strand, within Pf1N1B4_1033 at 1175.095 kb on + strand, within Pf1N1B4_1034 at 1175.105 kb on + strand, within Pf1N1B4_1034 at 1175.105 kb on + strand, within Pf1N1B4_1034 at 1175.106 kb on - strand, within Pf1N1B4_1034 at 1175.106 kb on - strand, within Pf1N1B4_1034 at 1175.106 kb on - strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.126 kb on + strand, within Pf1N1B4_1034 at 1175.127 kb on - strand, within Pf1N1B4_1034 at 1175.127 kb on - strand, within Pf1N1B4_1034 at 1175.127 kb on - strand at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.504 kb on + strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.505 kb on - strand, within Pf1N1B4_1035 at 1175.685 kb on - strand at 1175.685 kb on - strand at 1175.743 kb on + strand at 1175.751 kb on - strand at 1175.779 kb on + strand at 1175.792 kb on - strand at 1175.915 kb on + strand, within Pf1N1B4_1036 at 1175.916 kb on - strand, within Pf1N1B4_1036 at 1175.916 kb on - strand, within Pf1N1B4_1036 at 1175.916 kb on - strand, within Pf1N1B4_1036 at 1175.920 kb on - strand, within Pf1N1B4_1036 at 1175.949 kb on - strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.950 kb on + strand, within Pf1N1B4_1036 at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1175.951 kb on - strand at 1175.951 kb on - strand, within Pf1N1B4_1036 at 1176.152 kb on + strand, within Pf1N1B4_1036 at 1176.152 kb on + strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.153 kb on - strand, within Pf1N1B4_1036 at 1176.305 kb on - strand at 1176.544 kb on - strand, within Pf1N1B4_1037 at 1176.544 kb on - strand, within Pf1N1B4_1037 at 1176.591 kb on + strand, within Pf1N1B4_1037 at 1176.591 kb on + strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.592 kb on - strand, within Pf1N1B4_1037 at 1176.614 kb on + strand, within Pf1N1B4_1037 at 1176.614 kb on + strand, within Pf1N1B4_1037 at 1176.615 kb on - strand at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037 at 1176.615 kb on - strand, within Pf1N1B4_1037
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,174,261 + -0.6 1,174,268 + +0.3 1,174,268 + +1.1 1,174,269 - +0.6 1,174,269 - -1.1 1,174,269 - -1.1 1,174,432 - Pf1N1B4_1033 0.22 +0.2 1,174,460 + Pf1N1B4_1033 0.26 -1.1 1,174,460 + Pf1N1B4_1033 0.26 -0.6 1,174,460 + Pf1N1B4_1033 0.26 -0.5 1,174,460 + Pf1N1B4_1033 0.26 +0.7 1,174,461 - Pf1N1B4_1033 0.26 -2.4 1,174,461 - Pf1N1B4_1033 0.26 +0.1 1,174,461 - Pf1N1B4_1033 0.26 -0.4 1,174,461 - Pf1N1B4_1033 0.26 +1.1 1,174,597 + Pf1N1B4_1033 0.43 +2.1 1,174,598 - Pf1N1B4_1033 0.43 +0.7 1,174,598 - Pf1N1B4_1033 0.43 -2.1 1,174,606 + Pf1N1B4_1033 0.44 +0.2 1,174,606 + Pf1N1B4_1033 0.44 -2.2 1,174,607 - Pf1N1B4_1033 0.44 +0.1 1,174,707 + Pf1N1B4_1033 0.57 -0.9 1,174,708 - Pf1N1B4_1033 0.57 +0.3 1,174,708 - Pf1N1B4_1033 0.57 +1.5 1,174,708 - Pf1N1B4_1033 0.57 -1.9 1,174,708 - Pf1N1B4_1033 0.57 -1.2 1,174,756 + Pf1N1B4_1033 0.63 +0.6 1,174,778 - Pf1N1B4_1033 0.66 +0.6 1,174,778 - Pf1N1B4_1033 0.66 -0.3 1,175,095 + Pf1N1B4_1034 0.31 -0.5 1,175,105 + Pf1N1B4_1034 0.34 -2.3 1,175,105 + Pf1N1B4_1034 0.34 -0.3 1,175,106 - Pf1N1B4_1034 0.35 -1.4 1,175,106 - Pf1N1B4_1034 0.35 -1.0 1,175,106 - Pf1N1B4_1034 0.35 -2.0 1,175,126 + Pf1N1B4_1034 0.42 -0.3 1,175,126 + Pf1N1B4_1034 0.42 +0.7 1,175,126 + Pf1N1B4_1034 0.42 -1.2 1,175,126 + Pf1N1B4_1034 0.42 -0.3 1,175,126 + Pf1N1B4_1034 0.42 -0.9 1,175,126 + Pf1N1B4_1034 0.42 +0.6 1,175,126 + Pf1N1B4_1034 0.42 +1.4 1,175,127 - Pf1N1B4_1034 0.43 -0.9 1,175,127 - Pf1N1B4_1034 0.43 -0.3 1,175,127 - +1.1 1,175,504 + Pf1N1B4_1035 0.54 -1.4 1,175,504 + Pf1N1B4_1035 0.54 -1.2 1,175,504 + +0.1 1,175,504 + Pf1N1B4_1035 0.54 -0.4 1,175,504 + Pf1N1B4_1035 0.54 -0.9 1,175,504 + Pf1N1B4_1035 0.54 -1.4 1,175,505 - Pf1N1B4_1035 0.54 -1.4 1,175,505 - Pf1N1B4_1035 0.54 -1.1 1,175,505 - Pf1N1B4_1035 0.54 -0.3 1,175,505 - Pf1N1B4_1035 0.54 -1.9 1,175,685 - -1.7 1,175,685 - -0.4 1,175,743 + -0.7 1,175,751 - -0.4 1,175,779 + -2.4 1,175,792 - +0.5 1,175,915 + Pf1N1B4_1036 0.20 +0.1 1,175,916 - Pf1N1B4_1036 0.20 -1.9 1,175,916 - Pf1N1B4_1036 0.20 +1.1 1,175,916 - Pf1N1B4_1036 0.20 +0.1 1,175,920 - Pf1N1B4_1036 0.21 +2.1 1,175,949 - Pf1N1B4_1036 0.27 +0.7 1,175,950 + Pf1N1B4_1036 0.27 +0.7 1,175,950 + Pf1N1B4_1036 0.27 +0.8 1,175,950 + Pf1N1B4_1036 0.27 +1.0 1,175,950 + Pf1N1B4_1036 0.27 -1.2 1,175,951 - Pf1N1B4_1036 0.28 -0.4 1,175,951 - Pf1N1B4_1036 0.28 +0.7 1,175,951 - Pf1N1B4_1036 0.28 -1.9 1,175,951 - +1.7 1,175,951 - Pf1N1B4_1036 0.28 -0.9 1,176,152 + Pf1N1B4_1036 0.70 -0.4 1,176,152 + Pf1N1B4_1036 0.70 -2.9 1,176,153 - Pf1N1B4_1036 0.70 +1.5 1,176,153 - Pf1N1B4_1036 0.70 -0.2 1,176,153 - Pf1N1B4_1036 0.70 -0.9 1,176,153 - Pf1N1B4_1036 0.70 -0.3 1,176,153 - Pf1N1B4_1036 0.70 -1.2 1,176,153 - Pf1N1B4_1036 0.70 -0.6 1,176,153 - Pf1N1B4_1036 0.70 -0.2 1,176,305 - +0.1 1,176,544 - Pf1N1B4_1037 0.32 -0.9 1,176,544 - Pf1N1B4_1037 0.32 -1.2 1,176,591 + Pf1N1B4_1037 0.40 +1.7 1,176,591 + Pf1N1B4_1037 0.40 -0.2 1,176,592 - Pf1N1B4_1037 0.40 -1.1 1,176,592 - Pf1N1B4_1037 0.40 -0.5 1,176,592 - Pf1N1B4_1037 0.40 +1.1 1,176,592 - Pf1N1B4_1037 0.40 -1.4 1,176,614 + Pf1N1B4_1037 0.44 +0.8 1,176,614 + Pf1N1B4_1037 0.44 -1.9 1,176,615 - -1.4 1,176,615 - Pf1N1B4_1037 0.44 +1.1 1,176,615 - Pf1N1B4_1037 0.44 -0.9 1,176,615 - Pf1N1B4_1037 0.44 -0.2 1,176,615 - Pf1N1B4_1037 0.44 -1.9 1,176,615 - Pf1N1B4_1037 0.44 -0.3 1,176,615 - Pf1N1B4_1037 0.44 -0.4 1,176,615 - Pf1N1B4_1037 0.44 +0.5
Or see this region's nucleotide sequence