Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1004 and Pf1N1B4_1005 are separated by 336 nucleotides Pf1N1B4_1005 and Pf1N1B4_1006 are separated by 21 nucleotides
Pf1N1B4_1004 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 1,138,974 to 1,139,948
_1004
Pf1N1B4_1005 - probable membrane protein YPO0899, at 1,140,285 to 1,140,650
_1005
Pf1N1B4_1006 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,140,672 to 1,141,574
_1006
Position (kb)
1140
1141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.489 kb on + strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.493 kb on + strand, within Pf1N1B4_1004 at 1139.760 kb on + strand, within Pf1N1B4_1004 at 1139.994 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.084 kb on - strand at 1140.100 kb on + strand at 1140.203 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.259 kb on - strand at 1140.259 kb on - strand at 1140.264 kb on + strand at 1140.312 kb on + strand at 1140.312 kb on + strand at 1140.313 kb on - strand at 1140.330 kb on - strand, within Pf1N1B4_1005 at 1140.415 kb on - strand, within Pf1N1B4_1005 at 1140.593 kb on + strand, within Pf1N1B4_1005 at 1140.841 kb on + strand at 1140.841 kb on + strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.922 kb on + strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1141.012 kb on + strand, within Pf1N1B4_1006 at 1141.012 kb on + strand, within Pf1N1B4_1006 at 1141.013 kb on - strand, within Pf1N1B4_1006 at 1141.013 kb on - strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.099 kb on - strand, within Pf1N1B4_1006 at 1141.099 kb on - strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.152 kb on - strand at 1141.152 kb on - strand at 1141.331 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.408 kb on - strand, within Pf1N1B4_1006 at 1141.554 kb on + strand at 1141.554 kb on + strand at 1141.554 kb on + strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,139,306 + Pf1N1B4_1004 0.34 -1.0 1,139,306 + Pf1N1B4_1004 0.34 -0.9 1,139,307 - Pf1N1B4_1004 0.34 +1.7 1,139,307 - Pf1N1B4_1004 0.34 -0.7 1,139,307 - Pf1N1B4_1004 0.34 -1.2 1,139,489 + Pf1N1B4_1004 0.53 -1.5 1,139,490 - Pf1N1B4_1004 0.53 +0.5 1,139,490 - Pf1N1B4_1004 0.53 -1.5 1,139,493 + Pf1N1B4_1004 0.53 -0.9 1,139,760 + Pf1N1B4_1004 0.81 +0.2 1,139,994 - -0.3 1,140,020 - +0.1 1,140,020 - -1.2 1,140,020 - -1.5 1,140,020 - -0.1 1,140,036 - +0.1 1,140,036 - -0.9 1,140,036 - +1.3 1,140,084 - -1.5 1,140,100 + -1.2 1,140,203 - -1.2 1,140,229 - -0.9 1,140,229 - -0.6 1,140,229 - -0.7 1,140,259 - +0.5 1,140,259 - -0.1 1,140,264 + +0.7 1,140,312 + +0.1 1,140,312 + +0.1 1,140,313 - -0.2 1,140,330 - Pf1N1B4_1005 0.12 +1.1 1,140,415 - Pf1N1B4_1005 0.36 +1.0 1,140,593 + Pf1N1B4_1005 0.84 -0.4 1,140,841 + +1.1 1,140,841 + Pf1N1B4_1006 0.19 +0.4 1,140,842 - Pf1N1B4_1006 0.19 +1.7 1,140,842 - Pf1N1B4_1006 0.19 -0.3 1,140,922 + Pf1N1B4_1006 0.28 -2.0 1,140,923 - Pf1N1B4_1006 0.28 +0.8 1,140,923 - Pf1N1B4_1006 0.28 +0.1 1,140,923 - Pf1N1B4_1006 0.28 -0.2 1,140,940 + Pf1N1B4_1006 0.30 -0.2 1,140,940 + Pf1N1B4_1006 0.30 +0.7 1,140,940 + Pf1N1B4_1006 0.30 -0.5 1,140,940 + Pf1N1B4_1006 0.30 -1.5 1,141,012 + Pf1N1B4_1006 0.38 -0.9 1,141,012 + Pf1N1B4_1006 0.38 +1.5 1,141,013 - Pf1N1B4_1006 0.38 -0.7 1,141,013 - Pf1N1B4_1006 0.38 +0.1 1,141,098 + Pf1N1B4_1006 0.47 -1.5 1,141,098 + Pf1N1B4_1006 0.47 -0.9 1,141,098 + Pf1N1B4_1006 0.47 -0.5 1,141,099 - Pf1N1B4_1006 0.47 -0.5 1,141,099 - Pf1N1B4_1006 0.47 +1.9 1,141,151 + Pf1N1B4_1006 0.53 -2.5 1,141,151 + Pf1N1B4_1006 0.53 -0.5 1,141,151 + Pf1N1B4_1006 0.53 +0.1 1,141,152 - +0.7 1,141,152 - +1.1 1,141,331 + Pf1N1B4_1006 0.73 -0.9 1,141,407 + Pf1N1B4_1006 0.81 +1.7 1,141,407 + Pf1N1B4_1006 0.81 -0.2 1,141,407 + Pf1N1B4_1006 0.81 +0.6 1,141,407 + Pf1N1B4_1006 0.81 -0.1 1,141,407 + Pf1N1B4_1006 0.81 -1.5 1,141,408 - Pf1N1B4_1006 0.82 +0.1 1,141,554 + -0.4 1,141,554 + -0.9 1,141,554 + -1.9 1,141,555 - +0.1 1,141,555 - -0.1 1,141,555 - -0.2 1,141,555 - -0.5 1,141,555 - -0.5 1,141,555 - -0.6 1,141,558 + -0.1 1,141,558 + +1.7 1,141,558 + +0.9 1,141,558 + -2.2 1,141,558 + +0.7 1,141,558 + -0.5 1,141,558 + +0.7 1,141,559 - +0.1 1,141,559 - -1.2 1,141,559 - -0.9 1,141,559 - +0.1
Or see this region's nucleotide sequence