Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1 and Pf1N1B4_2 are separated by 98 nucleotidesPf1N1B4_2 and Pf1N1B4_3 overlap by 17 nucleotides Pf1N1B4_1 - Colicin V production protein, at 1,060 to 1,620 _1 Pf1N1B4_2 - DedD protein, at 1,719 to 2,390 _2 Pf1N1B4_3 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 2,374 to 3,681 _3 Position (kb) 1 2Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 0.207 kb on + strandat 0.208 kb on - strandat 0.208 kb on - strandat 0.208 kb on - strandat 0.249 kb on + strandat 0.250 kb on - strandat 0.330 kb on + strandat 0.330 kb on + strandat 0.893 kb on + strandat 1.033 kb on - strandat 1.036 kb on + strandat 1.037 kb on - strandat 1.037 kb on - strandat 1.037 kb on - strandat 1.037 kb on - strandat 1.106 kb on - strandat 1.106 kb on - strandat 1.106 kb on - strandat 1.106 kb on - strandat 1.106 kb on - strandat 1.106 kb on - strandat 1.108 kb on - strandat 1.367 kb on + strand, within Pf1N1B4_1at 1.367 kb on + strand, within Pf1N1B4_1at 1.368 kb on - strand, within Pf1N1B4_1at 1.385 kb on - strand, within Pf1N1B4_1at 1.451 kb on + strand, within Pf1N1B4_1

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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207 + -2.6
208 - -2.6
208 - -4.3
208 - -0.3
249 + -1.9
250 - -0.3
330 + +0.7
330 + -3.1
893 + -3.5
1,033 - -1.9
1,036 + -1.0
1,037 - +0.7
1,037 - -0.9
1,037 - -0.6
1,037 - -0.3
1,106 - +0.3
1,106 - -1.3
1,106 - -0.0
1,106 - -1.3
1,106 - -0.3
1,106 - -0.3
1,108 - +1.1
1,367 + Pf1N1B4_1 0.55 -1.3
1,367 + Pf1N1B4_1 0.55 -1.3
1,368 - Pf1N1B4_1 0.55 -0.3
1,385 - Pf1N1B4_1 0.58 -2.1
1,451 + Pf1N1B4_1 0.70 -2.9

Or see this region's nucleotide sequence