Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS20705

Experiment: Temperature shift 30_to_10; with MOPS

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_10; with MOPS
4,661,813	+		PFLU_RS20700	0.50	-0.5
4,661,814	-		PFLU_RS20700	0.50	+0.7
4,661,904	+		PFLU_RS20700	0.60	+0.4
4,661,904	+		PFLU_RS20700	0.60	-0.4
4,661,904	+		PFLU_RS20700	0.60	-0.4
4,661,904	+		PFLU_RS20700	0.60	-1.7
4,661,904	+		PFLU_RS20700	0.60	-0.2
4,661,905	-		PFLU_RS20700	0.60	+0.6
4,661,905	-		PFLU_RS20700	0.60	+0.2
4,661,905	-		PFLU_RS20700	0.60	+1.1
4,661,905	-		PFLU_RS20700	0.60	-1.0
4,661,905	-		PFLU_RS20700	0.60	+0.2
4,661,952	-		PFLU_RS20700	0.65	-0.2
4,661,962	+		PFLU_RS20700	0.66	+0.2
4,662,030	+		PFLU_RS20700	0.74	+0.3
4,662,031	-		PFLU_RS20700	0.74	-0.2
4,662,066	+		PFLU_RS20700	0.77	+0.0
4,662,066	+		PFLU_RS20700	0.77	-0.2
4,662,066	+		PFLU_RS20700	0.77	-0.2
4,662,066	+		PFLU_RS20700	0.77	-0.5
4,662,066	+		PFLU_RS20700	0.77	+0.5
4,662,066	+		PFLU_RS20700	0.77	-0.2
4,662,067	-		PFLU_RS20700	0.78	+0.5
4,662,067	-		PFLU_RS20700	0.78	-0.2
4,662,067	-		PFLU_RS20700	0.78	+0.6
4,662,067	-		PFLU_RS20700	0.78	+0.1
4,662,067	-		PFLU_RS20700	0.78	+1.4
4,662,067	-		PFLU_RS20700	0.78	+0.2
4,662,129	+		PFLU_RS20700	0.84	-0.0
4,662,129	+		PFLU_RS20700	0.84	+0.6
4,662,193	-				-1.2
4,662,193	-				+1.2
4,662,247	+				+0.2
4,662,247	+				+0.5
4,662,247	+				+0.2
4,662,247	+				-1.6
4,662,247	+				+0.4
4,662,247	+				+0.7
4,662,247	+				-1.4
4,662,247	+				+0.5
4,662,247	+				-1.6
4,662,247	+				-0.3
4,662,247	+				+0.2
4,662,247	+				+1.7
4,662,247	+				+1.3
4,662,247	+				+0.2
4,662,247	+				+0.2
4,662,248	-				-0.5
4,662,248	-				+1.2
4,662,248	-				-0.5
4,662,248	-				+0.3
4,662,248	-				+0.4
4,662,248	-				+0.8
4,662,248	-				+2.1
4,662,248	-				+0.5
4,662,248	-				+1.0
4,662,248	-				-0.3
4,662,248	-				-0.0
4,662,248	-				+0.8
4,662,273	+				+1.1
4,662,273	+				+1.2
4,662,274	-				-0.1
4,662,274	-				-0.6
4,662,284	+				-2.5
4,662,284	+				+1.4
4,662,284	+				+1.8
4,662,284	+				+0.5
4,662,284	+				-1.6
4,662,284	+				-2.5
4,662,284	+				+1.4
4,662,284	+				-0.0
4,662,285	-				-1.1
4,662,285	-				-1.4
4,662,285	-				-2.5
4,662,285	-				+2.2
4,662,285	-				+0.4
4,662,285	-				-1.8
4,662,285	-				+0.9
4,662,285	-				-0.8
4,662,363	-				-0.1
4,662,577	+				+0.6
4,662,618	-				+0.5
4,662,618	-				+0.5
4,662,618	-				-1.3
4,662,618	-				+0.1
4,662,618	-				-0.7
4,662,618	-				+0.4
4,662,618	-				+1.0
4,662,621	+				-1.2
4,662,622	-				+1.0
4,662,622	-				-0.4
4,662,700	+				+0.9
4,662,710	+				-0.0
4,662,710	+				+0.6
4,662,710	+				-0.2
4,662,711	-				-0.4
4,662,711	-				-0.6
4,662,711	-				-1.2
4,662,748	+				-0.5
4,662,748	+				-2.2
4,662,748	+				-1.6
4,662,748	+				-0.7
4,662,748	+				-0.4
4,662,748	+				+1.7
4,662,748	+				+0.1
4,662,749	-				-0.2
4,662,749	-				-0.8
4,662,749	-				-0.3
4,662,749	-				+0.2
4,662,749	-				+0.5
4,662,749	-				-1.6
4,662,749	-				-0.2
4,662,749	-				-1.4
4,662,749	-				-1.2
4,662,817	+		PFLU_RS20705	0.19	-0.2
4,662,820	+		PFLU_RS20705	0.20	+0.2
4,662,820	+		PFLU_RS20705	0.20	-0.2
4,662,820	+		PFLU_RS20705	0.20	-0.0
4,662,820	+		PFLU_RS20705	0.20	+0.9
4,662,821	-		PFLU_RS20705	0.20	-0.8
4,662,821	-		PFLU_RS20705	0.20	-0.0
4,662,821	-		PFLU_RS20705	0.20	+0.8
4,662,821	-		PFLU_RS20705	0.20	-0.0
4,662,821	-		PFLU_RS20705	0.20	+0.2
4,662,929	+		PFLU_RS20705	0.44	-1.6
4,662,930	-		PFLU_RS20705	0.45	-0.2
4,662,950	+		PFLU_RS20705	0.49	-1.6
4,663,076	-		PFLU_RS20705	0.77	-0.0
4,663,076	-		PFLU_RS20705	0.77	-0.5
4,663,076	-		PFLU_RS20705	0.77	+0.3
4,663,115	+		PFLU_RS20705	0.86	+0.3
4,663,116	-		PFLU_RS20705	0.86	+0.5
4,663,167	+				-0.0
4,663,180	+				+0.6
4,663,180	+				+0.7
4,663,180	+				-2.7
4,663,181	-				+1.2
4,663,181	-				+0.2
4,663,193	-				-0.1
4,663,218	+				-2.7
4,663,218	+				+0.1
4,663,218	+				-0.8
4,663,219	-				-0.7
4,663,219	-				-1.0
4,663,219	-				+2.2
4,663,219	-				-1.5
4,663,219	-				-0.5
4,663,283	+				-0.1
4,663,284	-				-1.0
4,663,284	-				+0.8
4,663,288	+				+0.4
4,663,313	+				+0.1
4,663,313	+				+0.4
4,663,313	+				+0.1
4,663,313	+				+0.4
4,663,314	-				-1.4
4,663,314	-				+0.8
4,663,314	-				+0.3
4,663,314	-				-0.6
4,663,316	+				-0.5
4,663,316	+				-0.4
4,663,316	+				-1.2
4,663,317	-				-2.3
4,663,370	+				+1.4
4,663,370	+				-0.5
4,663,370	+				-1.4
4,663,370	+				+1.0
4,663,370	+				-0.0
4,663,370	+				-1.7
4,663,370	+				+0.7
4,663,370	+				-0.2
4,663,370	+				+0.4
4,663,371	-				-0.4
4,663,371	-				-0.1
4,663,371	-				+1.2
4,663,371	-				+0.4
4,663,371	-				-0.2
4,663,371	-				+0.1
4,663,371	-				+0.2
4,663,371	-				-0.1
4,663,371	-				+0.9
4,663,371	-				+1.0
4,663,515	+				-0.0
4,663,516	-				+0.5
4,663,535	+				+1.1
4,663,535	+				+1.6
4,663,535	+				-0.6
4,663,535	+				+1.2
4,663,536	-				-0.5
4,663,536	-				+1.0
4,663,536	-				+2.2
4,663,536	-				-2.0
4,663,548	+				+2.0
4,663,548	+				+0.5
4,663,548	+				+0.5
4,663,548	+				+0.3
4,663,548	+				+1.7
4,663,548	+				-0.1
4,663,549	-				-1.1
4,663,549	-				-0.4
4,663,549	-				-1.4
4,663,566	+				-0.1
4,663,566	+				-0.7
4,663,566	+				-0.2
4,663,566	+				+1.9
4,663,566	+				+2.5
4,663,566	+				+0.7
4,663,566	+				-1.7
4,663,567	-				+0.7
4,663,567	-				-0.7
4,663,567	-				-0.6
4,663,567	-				-0.7
4,663,567	-				-0.8
4,663,567	-				-1.5
4,663,691	+		PFLU_RS20710	0.10	-0.4
4,663,692	-		PFLU_RS20710	0.10	+0.8
4,663,692	-		PFLU_RS20710	0.10	-0.0
4,663,759	-		PFLU_RS20710	0.17	+0.1
4,663,779	-		PFLU_RS20710	0.19	-1.7
4,663,824	+		PFLU_RS20710	0.23	+0.1
4,663,824	+		PFLU_RS20710	0.23	-0.2
4,663,824	+		PFLU_RS20710	0.23	+0.8
4,663,824	+		PFLU_RS20710	0.23	+1.5
4,663,824	+		PFLU_RS20710	0.23	-1.8
4,663,824	+		PFLU_RS20710	0.23	+1.4
4,663,824	+		PFLU_RS20710	0.23	+0.6
4,663,825	-		PFLU_RS20710	0.23	+0.0
4,663,825	-		PFLU_RS20710	0.23	+0.5
4,663,825	-		PFLU_RS20710	0.23	-0.2
4,663,825	-		PFLU_RS20710	0.23	-0.1
4,663,825	-		PFLU_RS20710	0.23	-0.1
4,663,825	-		PFLU_RS20710	0.23	-0.2
4,663,825	-		PFLU_RS20710	0.23	-1.1
4,663,992	+		PFLU_RS20710	0.40	-0.8
4,663,992	+		PFLU_RS20710	0.40	+0.5
4,663,992	+		PFLU_RS20710	0.40	+0.3
4,663,992	+		PFLU_RS20710	0.40	+0.1
4,663,992	+		PFLU_RS20710	0.40	+0.5
4,663,992	+		PFLU_RS20710	0.40	+0.2
4,663,993	-		PFLU_RS20710	0.40	+0.5
4,663,993	-		PFLU_RS20710	0.40	-0.4
4,663,993	-		PFLU_RS20710	0.40	-0.3
4,663,993	-		PFLU_RS20710	0.40	-0.1
4,663,993	-		PFLU_RS20710	0.40	-1.1
4,663,993	-		PFLU_RS20710	0.40	-0.4
4,663,993	-		PFLU_RS20710	0.40	-0.8
4,663,993	-		PFLU_RS20710	0.40	+1.6
4,663,993	-		PFLU_RS20710	0.40	-1.1
4,663,993	-		PFLU_RS20710	0.40	+0.7
4,664,079	+		PFLU_RS20710	0.48	-1.2
4,664,079	+		PFLU_RS20710	0.48	+1.3
4,664,080	-		PFLU_RS20710	0.48	-1.2
4,664,080	-		PFLU_RS20710	0.48	-0.5
4,664,080	-		PFLU_RS20710	0.48	+0.3
4,664,082	-		PFLU_RS20710	0.49	-0.5
4,664,082	-		PFLU_RS20710	0.49	+0.1
4,664,082	-		PFLU_RS20710	0.49	-1.0
4,664,116	+		PFLU_RS20710	0.52	+0.2
4,664,116	+		PFLU_RS20710	0.52	+1.2
4,664,117	-		PFLU_RS20710	0.52	+0.1