Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06314 and H281DRAFT_06315 are separated by 107 nucleotides H281DRAFT_06315 and H281DRAFT_06316 are separated by 256 nucleotides H281DRAFT_06316 and H281DRAFT_06317 are separated by 158 nucleotides
H281DRAFT_06314: H281DRAFT_06314 - transketolase, at 97,260 to 99,281
_06314
H281DRAFT_06315: H281DRAFT_06315 - glyceraldehyde-3-phosphate dehydrogenase (NAD+), at 99,389 to 100,399
_06315
H281DRAFT_06316: H281DRAFT_06316 - ferric uptake regulator, Fur family, at 100,656 to 101,084
_06316
H281DRAFT_06317: H281DRAFT_06317 - Beta-barrel assembly machine subunit BamE, at 101,243 to 102,067
_06317
Position (kb) 99
100
101 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 99.353 kb on + strand at 99.362 kb on - strand at 99.383 kb on + strand at 99.384 kb on - strand at 99.452 kb on + strand at 99.453 kb on - strand at 99.537 kb on - strand, within H281DRAFT_06315 at 99.794 kb on + strand at 99.969 kb on - strand, within H281DRAFT_06315 at 100.332 kb on - strand at 100.407 kb on + strand at 100.407 kb on + strand at 100.408 kb on - strand at 100.408 kb on - strand at 100.408 kb on - strand at 100.408 kb on - strand at 100.432 kb on + strand at 100.451 kb on + strand at 100.515 kb on + strand at 101.124 kb on + strand at 101.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 99,353 + -0.3 99,362 - -3.2 99,383 + +1.7 99,384 - +0.5 99,452 + -0.9 99,453 - -0.3 99,537 - H281DRAFT_06315 0.15 -1.2 99,794 + -0.5 99,969 - H281DRAFT_06315 0.57 -4.1 100,332 - +0.1 100,407 + -2.2 100,407 + -0.1 100,408 - +1.6 100,408 - -0.3 100,408 - +0.7 100,408 - -0.5 100,432 + -0.4 100,451 + +0.4 100,515 + -0.3 101,124 + +0.2 101,124 + +0.3
Or see this region's nucleotide sequence