Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06115 and H281DRAFT_06116 are separated by 73 nucleotides H281DRAFT_06116 and H281DRAFT_06117 are separated by 152 nucleotides
H281DRAFT_06115: H281DRAFT_06115 - Protein of unknown function (DUF1479), at 134,670 to 135,920
_06115
H281DRAFT_06116: H281DRAFT_06116 - transcriptional regulator, AraC family, at 135,994 to 137,157
_06116
H281DRAFT_06117: H281DRAFT_06117 - citrate synthase, at 137,310 to 138,611
_06117
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -2
-1
0
1
2 at 135.082 kb on + strand, within H281DRAFT_06115 at 135.241 kb on + strand, within H281DRAFT_06115 at 135.241 kb on + strand, within H281DRAFT_06115 at 135.515 kb on - strand, within H281DRAFT_06115 at 135.515 kb on - strand, within H281DRAFT_06115 at 135.515 kb on - strand, within H281DRAFT_06115 at 135.515 kb on - strand, within H281DRAFT_06115 at 135.515 kb on - strand, within H281DRAFT_06115 at 135.559 kb on + strand, within H281DRAFT_06115 at 135.560 kb on - strand, within H281DRAFT_06115 at 135.560 kb on - strand, within H281DRAFT_06115 at 135.613 kb on + strand, within H281DRAFT_06115 at 135.743 kb on - strand, within H281DRAFT_06115 at 135.743 kb on - strand, within H281DRAFT_06115 at 135.743 kb on - strand, within H281DRAFT_06115 at 135.844 kb on + strand at 135.844 kb on + strand at 135.844 kb on + strand at 135.845 kb on - strand at 135.911 kb on + strand at 135.911 kb on + strand at 135.912 kb on - strand at 135.912 kb on - strand at 135.912 kb on - strand at 135.935 kb on - strand at 135.969 kb on + strand at 135.969 kb on + strand at 135.970 kb on - strand at 135.999 kb on + strand at 136.000 kb on - strand at 136.000 kb on - strand at 136.000 kb on - strand at 136.079 kb on - strand at 136.120 kb on + strand, within H281DRAFT_06116 at 136.121 kb on - strand, within H281DRAFT_06116 at 136.121 kb on - strand, within H281DRAFT_06116 at 136.278 kb on + strand, within H281DRAFT_06116 at 136.278 kb on + strand, within H281DRAFT_06116 at 136.579 kb on - strand, within H281DRAFT_06116 at 136.844 kb on - strand, within H281DRAFT_06116 at 136.844 kb on - strand, within H281DRAFT_06116 at 136.879 kb on + strand, within H281DRAFT_06116 at 136.880 kb on - strand, within H281DRAFT_06116 at 136.884 kb on - strand, within H281DRAFT_06116 at 136.884 kb on - strand, within H281DRAFT_06116 at 136.947 kb on - strand, within H281DRAFT_06116 at 137.142 kb on + strand at 137.142 kb on + strand at 137.192 kb on - strand at 137.192 kb on - strand at 137.192 kb on - strand at 137.272 kb on - strand at 137.311 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 135,082 + H281DRAFT_06115 0.33 +1.3 135,241 + H281DRAFT_06115 0.46 +0.6 135,241 + H281DRAFT_06115 0.46 -0.5 135,515 - H281DRAFT_06115 0.68 -1.2 135,515 - H281DRAFT_06115 0.68 -0.2 135,515 - H281DRAFT_06115 0.68 -0.0 135,515 - H281DRAFT_06115 0.68 +0.7 135,515 - H281DRAFT_06115 0.68 +0.3 135,559 + H281DRAFT_06115 0.71 +1.0 135,560 - H281DRAFT_06115 0.71 +1.5 135,560 - H281DRAFT_06115 0.71 -0.2 135,613 + H281DRAFT_06115 0.75 +0.3 135,743 - H281DRAFT_06115 0.86 -0.5 135,743 - H281DRAFT_06115 0.86 -0.4 135,743 - H281DRAFT_06115 0.86 -0.8 135,844 + -1.1 135,844 + +0.4 135,844 + +0.7 135,845 - +0.2 135,911 + +0.1 135,911 + -0.5 135,912 - +0.8 135,912 - -0.7 135,912 - -0.9 135,935 - +0.2 135,969 + -0.3 135,969 + +0.3 135,970 - +0.2 135,999 + -0.4 136,000 - +0.6 136,000 - +0.3 136,000 - -0.6 136,079 - +1.7 136,120 + H281DRAFT_06116 0.11 -0.3 136,121 - H281DRAFT_06116 0.11 +0.1 136,121 - H281DRAFT_06116 0.11 -1.4 136,278 + H281DRAFT_06116 0.24 -0.1 136,278 + H281DRAFT_06116 0.24 -0.3 136,579 - H281DRAFT_06116 0.50 +0.6 136,844 - H281DRAFT_06116 0.73 +1.3 136,844 - H281DRAFT_06116 0.73 -0.4 136,879 + H281DRAFT_06116 0.76 -0.2 136,880 - H281DRAFT_06116 0.76 -0.4 136,884 - H281DRAFT_06116 0.76 +0.5 136,884 - H281DRAFT_06116 0.76 +2.4 136,947 - H281DRAFT_06116 0.82 -2.3 137,142 + -0.2 137,142 + -1.1 137,192 - -0.2 137,192 - +0.2 137,192 - +0.6 137,272 - +0.9 137,311 + -0.0
Or see this region's nucleotide sequence