Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06080 and H281DRAFT_06081 are separated by 59 nucleotides H281DRAFT_06081 and H281DRAFT_06082 are separated by 53 nucleotides H281DRAFT_06082 and H281DRAFT_06083 are separated by 174 nucleotides H281DRAFT_06083 and H281DRAFT_06084 are separated by 143 nucleotides
H281DRAFT_06080: H281DRAFT_06080 - thioredoxin reductase (NADPH), at 96,856 to 98,091
_06080
H281DRAFT_06081: H281DRAFT_06081 - ubiquitin-hydrolase Zn-finger-containing protein, at 98,151 to 98,423
_06081
H281DRAFT_06082: H281DRAFT_06082 - ATP-dependent Clp protease ATP-binding subunit ClpC, at 98,477 to 101,311
_06082
H281DRAFT_06083: H281DRAFT_06083 - heat shock protein Hsp20, at 101,486 to 102,121
_06083
H281DRAFT_06084: H281DRAFT_06084 - Uncharacterized membrane protein HdeD, DUF308 family, at 102,265 to 102,840
_06084
Position (kb) 98
99
100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 97.498 kb on + strand, within H281DRAFT_06080 at 97.501 kb on + strand, within H281DRAFT_06080 at 97.547 kb on + strand, within H281DRAFT_06080 at 97.547 kb on + strand, within H281DRAFT_06080 at 97.547 kb on + strand, within H281DRAFT_06080 at 97.548 kb on - strand, within H281DRAFT_06080 at 97.549 kb on + strand, within H281DRAFT_06080 at 97.549 kb on + strand, within H281DRAFT_06080 at 97.549 kb on + strand, within H281DRAFT_06080 at 97.549 kb on + strand, within H281DRAFT_06080 at 97.549 kb on + strand, within H281DRAFT_06080 at 97.549 kb on + strand, within H281DRAFT_06080 at 97.619 kb on - strand, within H281DRAFT_06080 at 97.619 kb on - strand, within H281DRAFT_06080 at 97.699 kb on + strand, within H281DRAFT_06080 at 97.704 kb on - strand, within H281DRAFT_06080 at 97.886 kb on - strand, within H281DRAFT_06080 at 97.886 kb on - strand, within H281DRAFT_06080 at 97.971 kb on + strand at 97.972 kb on - strand at 97.972 kb on - strand at 97.972 kb on - strand at 97.972 kb on - strand at 98.082 kb on + strand at 98.128 kb on + strand at 98.128 kb on + strand at 98.144 kb on + strand at 98.145 kb on - strand at 98.245 kb on + strand, within H281DRAFT_06081 at 98.252 kb on - strand, within H281DRAFT_06081 at 99.188 kb on + strand, within H281DRAFT_06082 at 99.189 kb on - strand, within H281DRAFT_06082 at 99.638 kb on + strand, within H281DRAFT_06082 at 99.805 kb on - strand, within H281DRAFT_06082 at 99.854 kb on + strand, within H281DRAFT_06082 at 99.978 kb on + strand, within H281DRAFT_06082 at 99.978 kb on + strand, within H281DRAFT_06082 at 99.978 kb on + strand, within H281DRAFT_06082 at 99.979 kb on - strand, within H281DRAFT_06082 at 99.996 kb on - strand, within H281DRAFT_06082 at 100.105 kb on + strand, within H281DRAFT_06082 at 100.198 kb on - strand, within H281DRAFT_06082 at 100.225 kb on + strand, within H281DRAFT_06082 at 100.624 kb on + strand, within H281DRAFT_06082 at 100.641 kb on + strand, within H281DRAFT_06082 at 100.938 kb on + strand, within H281DRAFT_06082 at 101.042 kb on - strand at 101.080 kb on + strand at 101.215 kb on - strand at 101.649 kb on + strand, within H281DRAFT_06083 at 101.876 kb on + strand, within H281DRAFT_06083 at 102.275 kb on - strand at 102.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 97,498 + H281DRAFT_06080 0.52 +0.0 97,501 + H281DRAFT_06080 0.52 -0.1 97,547 + H281DRAFT_06080 0.56 -1.0 97,547 + H281DRAFT_06080 0.56 -0.7 97,547 + H281DRAFT_06080 0.56 +2.0 97,548 - H281DRAFT_06080 0.56 +0.3 97,549 + H281DRAFT_06080 0.56 +0.3 97,549 + H281DRAFT_06080 0.56 +0.3 97,549 + H281DRAFT_06080 0.56 +0.3 97,549 + H281DRAFT_06080 0.56 +0.1 97,549 + H281DRAFT_06080 0.56 +0.6 97,549 + H281DRAFT_06080 0.56 +0.8 97,619 - H281DRAFT_06080 0.62 +0.6 97,619 - H281DRAFT_06080 0.62 +0.3 97,699 + H281DRAFT_06080 0.68 -1.3 97,704 - H281DRAFT_06080 0.69 +1.1 97,886 - H281DRAFT_06080 0.83 +0.8 97,886 - H281DRAFT_06080 0.83 -0.7 97,971 + +0.0 97,972 - +0.4 97,972 - -3.0 97,972 - +0.2 97,972 - +0.8 98,082 + +1.1 98,128 + -1.4 98,128 + -0.2 98,144 + +1.4 98,145 - -3.6 98,245 + H281DRAFT_06081 0.34 -1.7 98,252 - H281DRAFT_06081 0.37 -0.3 99,188 + H281DRAFT_06082 0.25 -0.0 99,189 - H281DRAFT_06082 0.25 +0.0 99,638 + H281DRAFT_06082 0.41 +0.8 99,805 - H281DRAFT_06082 0.47 +0.3 99,854 + H281DRAFT_06082 0.49 -1.0 99,978 + H281DRAFT_06082 0.53 +0.4 99,978 + H281DRAFT_06082 0.53 +1.1 99,978 + H281DRAFT_06082 0.53 -0.9 99,979 - H281DRAFT_06082 0.53 +0.2 99,996 - H281DRAFT_06082 0.54 -1.1 100,105 + H281DRAFT_06082 0.57 -0.9 100,198 - H281DRAFT_06082 0.61 -0.3 100,225 + H281DRAFT_06082 0.62 -0.1 100,624 + H281DRAFT_06082 0.76 +0.5 100,641 + H281DRAFT_06082 0.76 +0.2 100,938 + H281DRAFT_06082 0.87 +0.5 101,042 - -0.3 101,080 + +1.0 101,215 - +1.0 101,649 + H281DRAFT_06083 0.26 -0.7 101,876 + H281DRAFT_06083 0.61 +0.0 102,275 - +0.7 102,275 - -1.2
Or see this region's nucleotide sequence