Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06065 and H281DRAFT_06066 overlap by 4 nucleotides H281DRAFT_06066 and H281DRAFT_06067 are separated by 11 nucleotides H281DRAFT_06067 and H281DRAFT_06068 are separated by 52 nucleotides H281DRAFT_06068 and H281DRAFT_06069 overlap by 11 nucleotides
H281DRAFT_06065: H281DRAFT_06065 - tryptophan synthase, alpha chain, at 79,947 to 80,813
_06065
H281DRAFT_06066: H281DRAFT_06066 - site-specific DNA-methyltransferase (adenine-specific), at 80,810 to 81,721
_06066
H281DRAFT_06067: H281DRAFT_06067 - tryptophan synthase, beta chain, at 81,733 to 82,926
_06067
H281DRAFT_06068: H281DRAFT_06068 - phosphoribosylanthranilate isomerase, at 82,979 to 83,704
_06068
H281DRAFT_06069: H281DRAFT_06069 - tRNA pseudouridine38-40 synthase, at 83,694 to 84,512
_06069
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 80.910 kb on + strand, within H281DRAFT_06066 at 81.130 kb on + strand, within H281DRAFT_06066 at 81.132 kb on + strand, within H281DRAFT_06066 at 81.132 kb on + strand, within H281DRAFT_06066 at 81.133 kb on - strand, within H281DRAFT_06066 at 81.133 kb on - strand, within H281DRAFT_06066 at 81.403 kb on + strand, within H281DRAFT_06066 at 81.405 kb on + strand, within H281DRAFT_06066 at 81.405 kb on + strand, within H281DRAFT_06066 at 81.406 kb on - strand, within H281DRAFT_06066 at 81.406 kb on - strand, within H281DRAFT_06066 at 81.406 kb on - strand, within H281DRAFT_06066 at 81.406 kb on - strand, within H281DRAFT_06066 at 81.406 kb on - strand, within H281DRAFT_06066 at 81.525 kb on + strand, within H281DRAFT_06066 at 81.575 kb on + strand, within H281DRAFT_06066 at 81.576 kb on - strand, within H281DRAFT_06066 at 81.576 kb on - strand, within H281DRAFT_06066 at 81.576 kb on - strand, within H281DRAFT_06066 at 81.677 kb on + strand at 81.677 kb on + strand at 81.678 kb on - strand at 81.765 kb on + strand at 81.848 kb on + strand at 81.939 kb on + strand, within H281DRAFT_06067 at 81.948 kb on - strand, within H281DRAFT_06067 at 82.002 kb on - strand, within H281DRAFT_06067 at 82.064 kb on + strand, within H281DRAFT_06067 at 82.064 kb on + strand, within H281DRAFT_06067 at 82.065 kb on - strand, within H281DRAFT_06067 at 82.065 kb on - strand, within H281DRAFT_06067 at 82.068 kb on + strand, within H281DRAFT_06067 at 82.068 kb on + strand, within H281DRAFT_06067 at 82.068 kb on + strand, within H281DRAFT_06067 at 82.069 kb on - strand, within H281DRAFT_06067 at 82.069 kb on - strand, within H281DRAFT_06067 at 82.207 kb on - strand, within H281DRAFT_06067 at 82.360 kb on - strand, within H281DRAFT_06067 at 82.361 kb on + strand, within H281DRAFT_06067 at 82.361 kb on + strand, within H281DRAFT_06067 at 82.361 kb on + strand, within H281DRAFT_06067 at 82.361 kb on + strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.362 kb on - strand, within H281DRAFT_06067 at 82.520 kb on + strand, within H281DRAFT_06067 at 82.520 kb on + strand, within H281DRAFT_06067 at 82.520 kb on + strand, within H281DRAFT_06067 at 82.520 kb on + strand, within H281DRAFT_06067 at 82.521 kb on - strand, within H281DRAFT_06067 at 82.521 kb on - strand, within H281DRAFT_06067 at 82.659 kb on + strand, within H281DRAFT_06067 at 82.660 kb on - strand, within H281DRAFT_06067 at 82.769 kb on + strand, within H281DRAFT_06067 at 82.787 kb on + strand, within H281DRAFT_06067 at 82.838 kb on - strand at 82.838 kb on - strand at 82.939 kb on + strand at 82.940 kb on - strand at 83.057 kb on + strand at 83.057 kb on + strand, within H281DRAFT_06068 at 83.176 kb on + strand, within H281DRAFT_06068 at 83.177 kb on - strand, within H281DRAFT_06068 at 83.257 kb on - strand, within H281DRAFT_06068 at 83.273 kb on + strand, within H281DRAFT_06068 at 83.571 kb on - strand, within H281DRAFT_06068 at 83.724 kb on + strand at 83.725 kb on - strand at 83.730 kb on - strand at 83.800 kb on + strand, within H281DRAFT_06069 at 83.801 kb on - strand, within H281DRAFT_06069 at 83.900 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 80,910 + H281DRAFT_06066 0.11 -1.1 81,130 + H281DRAFT_06066 0.35 -1.2 81,132 + H281DRAFT_06066 0.35 +0.2 81,132 + H281DRAFT_06066 0.35 -0.8 81,133 - H281DRAFT_06066 0.35 -0.3 81,133 - H281DRAFT_06066 0.35 -0.1 81,403 + H281DRAFT_06066 0.65 -0.5 81,405 + H281DRAFT_06066 0.65 -3.5 81,405 + H281DRAFT_06066 0.65 -1.3 81,406 - H281DRAFT_06066 0.65 -0.7 81,406 - H281DRAFT_06066 0.65 -0.6 81,406 - H281DRAFT_06066 0.65 -0.5 81,406 - H281DRAFT_06066 0.65 -0.7 81,406 - H281DRAFT_06066 0.65 -0.1 81,525 + H281DRAFT_06066 0.78 +0.0 81,575 + H281DRAFT_06066 0.84 -0.8 81,576 - H281DRAFT_06066 0.84 -0.2 81,576 - H281DRAFT_06066 0.84 -1.4 81,576 - H281DRAFT_06066 0.84 -1.8 81,677 + -1.5 81,677 + -2.1 81,678 - +0.2 81,765 + -3.2 81,848 + -3.2 81,939 + H281DRAFT_06067 0.17 -3.5 81,948 - H281DRAFT_06067 0.18 -2.7 82,002 - H281DRAFT_06067 0.23 -2.6 82,064 + H281DRAFT_06067 0.28 -3.6 82,064 + H281DRAFT_06067 0.28 -4.1 82,065 - H281DRAFT_06067 0.28 -2.2 82,065 - H281DRAFT_06067 0.28 -0.6 82,068 + H281DRAFT_06067 0.28 -3.4 82,068 + H281DRAFT_06067 0.28 -3.0 82,068 + H281DRAFT_06067 0.28 -5.8 82,069 - H281DRAFT_06067 0.28 -1.6 82,069 - H281DRAFT_06067 0.28 -3.4 82,207 - H281DRAFT_06067 0.40 -5.6 82,360 - H281DRAFT_06067 0.53 -4.2 82,361 + H281DRAFT_06067 0.53 -3.0 82,361 + H281DRAFT_06067 0.53 -3.7 82,361 + H281DRAFT_06067 0.53 -4.5 82,361 + H281DRAFT_06067 0.53 -0.0 82,362 - H281DRAFT_06067 0.53 -3.9 82,362 - H281DRAFT_06067 0.53 -2.2 82,362 - H281DRAFT_06067 0.53 -3.3 82,362 - H281DRAFT_06067 0.53 -4.2 82,362 - H281DRAFT_06067 0.53 -4.4 82,362 - H281DRAFT_06067 0.53 -3.6 82,362 - H281DRAFT_06067 0.53 -3.8 82,520 + H281DRAFT_06067 0.66 -5.2 82,520 + H281DRAFT_06067 0.66 -5.2 82,520 + H281DRAFT_06067 0.66 -3.6 82,520 + H281DRAFT_06067 0.66 -4.3 82,521 - H281DRAFT_06067 0.66 -4.9 82,521 - H281DRAFT_06067 0.66 -2.8 82,659 + H281DRAFT_06067 0.78 -4.3 82,660 - H281DRAFT_06067 0.78 -2.2 82,769 + H281DRAFT_06067 0.87 -5.3 82,787 + H281DRAFT_06067 0.88 -4.2 82,838 - -4.8 82,838 - -3.8 82,939 + -2.2 82,940 - -0.5 83,057 + +1.0 83,057 + H281DRAFT_06068 0.11 -2.5 83,176 + H281DRAFT_06068 0.27 -2.1 83,177 - H281DRAFT_06068 0.27 -5.6 83,257 - H281DRAFT_06068 0.38 -4.1 83,273 + H281DRAFT_06068 0.40 -3.4 83,571 - H281DRAFT_06068 0.82 -5.1 83,724 + -0.1 83,725 - -0.9 83,730 - +0.2 83,800 + H281DRAFT_06069 0.13 +0.4 83,801 - H281DRAFT_06069 0.13 +0.7 83,900 - +2.3
Or see this region's nucleotide sequence