Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05812 and H281DRAFT_05813 overlap by 4 nucleotides H281DRAFT_05813 and H281DRAFT_05814 are separated by 201 nucleotides H281DRAFT_05814 and H281DRAFT_05815 are separated by 97 nucleotides H281DRAFT_05815 and H281DRAFT_05816 are separated by 116 nucleotides
H281DRAFT_05812: H281DRAFT_05812 - biotin-dependent carboxylase uncharacterized domain-containing protein, at 89,102 to 90,154
_05812
H281DRAFT_05813: H281DRAFT_05813 - sensor histidine kinase inhibitor, KipI family, at 90,151 to 90,804
_05813
H281DRAFT_05814: H281DRAFT_05814 - Predicted transcription regulator, contains HTH domain, MarR family, at 91,006 to 91,518
_05814
H281DRAFT_05815: H281DRAFT_05815 - 5,10-methenyltetrahydrofolate synthetase, at 91,616 to 92,158
_05815
H281DRAFT_05816: H281DRAFT_05816 - soluble lytic murein transglycosylase, at 92,275 to 94,203
_05816
Position (kb)
91
92 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 90.160 kb on + strand at 90.160 kb on + strand at 90.288 kb on - strand, within H281DRAFT_05813 at 90.390 kb on + strand, within H281DRAFT_05813 at 90.391 kb on - strand, within H281DRAFT_05813 at 90.391 kb on - strand, within H281DRAFT_05813 at 90.391 kb on - strand, within H281DRAFT_05813 at 90.392 kb on + strand, within H281DRAFT_05813 at 90.392 kb on + strand, within H281DRAFT_05813 at 90.392 kb on + strand, within H281DRAFT_05813 at 90.393 kb on - strand, within H281DRAFT_05813 at 90.393 kb on - strand, within H281DRAFT_05813 at 90.393 kb on - strand, within H281DRAFT_05813 at 90.431 kb on + strand, within H281DRAFT_05813 at 90.431 kb on + strand, within H281DRAFT_05813 at 90.432 kb on - strand, within H281DRAFT_05813 at 90.432 kb on - strand, within H281DRAFT_05813 at 90.432 kb on - strand, within H281DRAFT_05813 at 90.525 kb on - strand, within H281DRAFT_05813 at 90.890 kb on - strand at 90.890 kb on - strand at 90.890 kb on - strand at 90.890 kb on - strand at 90.905 kb on + strand at 90.906 kb on - strand at 90.923 kb on - strand at 90.923 kb on - strand at 90.963 kb on + strand at 91.247 kb on + strand, within H281DRAFT_05814 at 91.247 kb on + strand, within H281DRAFT_05814 at 91.247 kb on + strand, within H281DRAFT_05814 at 91.247 kb on + strand, within H281DRAFT_05814 at 91.247 kb on + strand, within H281DRAFT_05814 at 91.248 kb on - strand, within H281DRAFT_05814 at 91.248 kb on - strand, within H281DRAFT_05814 at 91.248 kb on - strand, within H281DRAFT_05814 at 91.248 kb on - strand, within H281DRAFT_05814 at 91.248 kb on - strand, within H281DRAFT_05814 at 91.248 kb on - strand, within H281DRAFT_05814 at 91.254 kb on + strand, within H281DRAFT_05814 at 91.254 kb on + strand at 91.255 kb on - strand, within H281DRAFT_05814 at 91.255 kb on - strand, within H281DRAFT_05814 at 91.270 kb on + strand, within H281DRAFT_05814 at 91.270 kb on + strand, within H281DRAFT_05814 at 91.290 kb on - strand, within H281DRAFT_05814 at 91.290 kb on - strand, within H281DRAFT_05814 at 91.294 kb on - strand, within H281DRAFT_05814 at 91.301 kb on - strand, within H281DRAFT_05814 at 91.305 kb on - strand, within H281DRAFT_05814 at 91.305 kb on - strand, within H281DRAFT_05814 at 91.305 kb on - strand, within H281DRAFT_05814 at 91.483 kb on + strand at 91.483 kb on + strand at 91.483 kb on + strand at 91.483 kb on + strand at 91.483 kb on + strand at 91.483 kb on + strand at 91.484 kb on - strand at 91.484 kb on - strand at 91.484 kb on - strand at 91.484 kb on - strand at 91.515 kb on - strand at 91.517 kb on - strand at 91.517 kb on - strand at 91.569 kb on + strand at 91.570 kb on - strand at 91.570 kb on - strand at 91.618 kb on - strand at 91.679 kb on + strand, within H281DRAFT_05815 at 91.679 kb on + strand, within H281DRAFT_05815 at 91.680 kb on - strand, within H281DRAFT_05815 at 91.680 kb on - strand, within H281DRAFT_05815 at 91.717 kb on - strand, within H281DRAFT_05815 at 91.842 kb on - strand, within H281DRAFT_05815 at 91.842 kb on - strand at 92.046 kb on + strand, within H281DRAFT_05815 at 92.047 kb on - strand, within H281DRAFT_05815 at 92.116 kb on + strand at 92.116 kb on + strand at 92.138 kb on + strand at 92.153 kb on + strand at 92.201 kb on + strand at 92.201 kb on + strand at 92.201 kb on + strand at 92.201 kb on + strand at 92.201 kb on + strand at 92.202 kb on - strand at 92.260 kb on + strand at 92.260 kb on + strand at 92.307 kb on + strand at 92.307 kb on + strand at 92.307 kb on + strand at 92.307 kb on + strand at 92.443 kb on + strand at 92.444 kb on - strand at 92.458 kb on + strand at 92.467 kb on + strand at 92.467 kb on + strand at 92.468 kb on - strand, within H281DRAFT_05816
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 90,160 + -0.2 90,160 + +0.3 90,288 - H281DRAFT_05813 0.21 -0.1 90,390 + H281DRAFT_05813 0.37 +0.3 90,391 - H281DRAFT_05813 0.37 -0.6 90,391 - H281DRAFT_05813 0.37 +0.4 90,391 - H281DRAFT_05813 0.37 +0.0 90,392 + H281DRAFT_05813 0.37 +1.0 90,392 + H281DRAFT_05813 0.37 -0.7 90,392 + H281DRAFT_05813 0.37 -0.6 90,393 - H281DRAFT_05813 0.37 +0.9 90,393 - H281DRAFT_05813 0.37 +1.0 90,393 - H281DRAFT_05813 0.37 +0.6 90,431 + H281DRAFT_05813 0.43 +1.0 90,431 + H281DRAFT_05813 0.43 +2.3 90,432 - H281DRAFT_05813 0.43 +1.0 90,432 - H281DRAFT_05813 0.43 +0.0 90,432 - H281DRAFT_05813 0.43 +0.2 90,525 - H281DRAFT_05813 0.57 +1.1 90,890 - -0.9 90,890 - +0.7 90,890 - -4.0 90,890 - -0.1 90,905 + +0.5 90,906 - -1.9 90,923 - +0.1 90,923 - +0.4 90,963 + -0.1 91,247 + H281DRAFT_05814 0.47 -2.5 91,247 + H281DRAFT_05814 0.47 +0.3 91,247 + H281DRAFT_05814 0.47 -2.1 91,247 + H281DRAFT_05814 0.47 +0.1 91,247 + H281DRAFT_05814 0.47 -1.2 91,248 - H281DRAFT_05814 0.47 -1.7 91,248 - H281DRAFT_05814 0.47 -4.5 91,248 - H281DRAFT_05814 0.47 -2.4 91,248 - H281DRAFT_05814 0.47 -4.3 91,248 - H281DRAFT_05814 0.47 -3.0 91,248 - H281DRAFT_05814 0.47 -2.2 91,254 + H281DRAFT_05814 0.48 -1.7 91,254 + -0.6 91,255 - H281DRAFT_05814 0.49 -3.9 91,255 - H281DRAFT_05814 0.49 -2.3 91,270 + H281DRAFT_05814 0.51 -1.9 91,270 + H281DRAFT_05814 0.51 -0.7 91,290 - H281DRAFT_05814 0.55 -2.6 91,290 - H281DRAFT_05814 0.55 -4.4 91,294 - H281DRAFT_05814 0.56 -1.6 91,301 - H281DRAFT_05814 0.58 -2.5 91,305 - H281DRAFT_05814 0.58 -1.7 91,305 - H281DRAFT_05814 0.58 -2.7 91,305 - H281DRAFT_05814 0.58 -0.9 91,483 + -1.3 91,483 + -2.2 91,483 + -1.6 91,483 + -0.4 91,483 + -1.6 91,483 + -2.0 91,484 - -1.4 91,484 - -0.7 91,484 - -2.5 91,484 - -2.4 91,515 - -3.8 91,517 - -0.2 91,517 - +3.0 91,569 + +0.2 91,570 - -0.2 91,570 - +1.5 91,618 - +0.1 91,679 + H281DRAFT_05815 0.12 -1.0 91,679 + H281DRAFT_05815 0.12 -1.1 91,680 - H281DRAFT_05815 0.12 -2.4 91,680 - H281DRAFT_05815 0.12 -2.3 91,717 - H281DRAFT_05815 0.19 -3.5 91,842 - H281DRAFT_05815 0.42 -2.5 91,842 - -1.6 92,046 + H281DRAFT_05815 0.79 -3.6 92,047 - H281DRAFT_05815 0.79 -1.9 92,116 + -1.6 92,116 + -3.3 92,138 + -1.5 92,153 + -3.5 92,201 + -0.4 92,201 + -0.4 92,201 + -1.3 92,201 + +0.4 92,201 + +3.8 92,202 - +1.4 92,260 + +0.7 92,260 + -0.2 92,307 + +0.7 92,307 + +0.9 92,307 + +0.0 92,307 + +0.9 92,443 + +1.1 92,444 - +1.3 92,458 + +0.2 92,467 + +1.9 92,467 + +1.6 92,468 - H281DRAFT_05816 0.10 +0.9
Or see this region's nucleotide sequence