Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05720 and H281DRAFT_05721 are separated by 201 nucleotides H281DRAFT_05721 and H281DRAFT_05722 are separated by 1 nucleotides H281DRAFT_05722 and H281DRAFT_05723 are separated by 53 nucleotides
H281DRAFT_05720: H281DRAFT_05720 - phenylacetate-CoA ligase, at 139,050 to 140,354
_05720
H281DRAFT_05721: H281DRAFT_05721 - acyl-CoA thioesterase, at 140,556 to 141,044
_05721
H281DRAFT_05722: H281DRAFT_05722 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18) (from data) , at 141,046 to 141,837
_05722
H281DRAFT_05723: H281DRAFT_05723 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) , at 141,891 to 143,093
_05723
Position (kb)
141
142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 140.131 kb on + strand at 140.236 kb on + strand at 140.237 kb on - strand at 140.255 kb on - strand at 140.301 kb on + strand at 140.301 kb on + strand at 140.302 kb on - strand at 140.302 kb on - strand at 140.391 kb on + strand at 140.557 kb on + strand at 140.558 kb on - strand at 140.558 kb on - strand at 140.648 kb on + strand, within H281DRAFT_05721 at 140.663 kb on + strand, within H281DRAFT_05721 at 140.663 kb on + strand, within H281DRAFT_05721 at 140.664 kb on - strand, within H281DRAFT_05721 at 140.664 kb on - strand, within H281DRAFT_05721 at 140.664 kb on - strand, within H281DRAFT_05721 at 140.664 kb on - strand, within H281DRAFT_05721 at 140.667 kb on + strand, within H281DRAFT_05721 at 140.668 kb on - strand, within H281DRAFT_05721 at 140.676 kb on - strand, within H281DRAFT_05721 at 140.680 kb on + strand, within H281DRAFT_05721 at 140.680 kb on + strand, within H281DRAFT_05721 at 140.680 kb on + strand, within H281DRAFT_05721 at 140.681 kb on - strand, within H281DRAFT_05721 at 140.681 kb on - strand, within H281DRAFT_05721 at 140.797 kb on + strand, within H281DRAFT_05721 at 140.798 kb on - strand, within H281DRAFT_05721 at 140.798 kb on - strand, within H281DRAFT_05721 at 140.798 kb on - strand, within H281DRAFT_05721 at 140.837 kb on + strand, within H281DRAFT_05721 at 140.837 kb on + strand, within H281DRAFT_05721 at 140.838 kb on - strand, within H281DRAFT_05721 at 140.841 kb on + strand, within H281DRAFT_05721 at 140.912 kb on - strand, within H281DRAFT_05721 at 140.972 kb on - strand, within H281DRAFT_05721 at 140.972 kb on - strand, within H281DRAFT_05721 at 141.025 kb on + strand at 141.025 kb on + strand at 141.044 kb on + strand at 141.045 kb on - strand at 141.045 kb on - strand at 141.045 kb on - strand at 141.104 kb on + strand at 141.104 kb on + strand at 141.104 kb on + strand at 141.199 kb on - strand, within H281DRAFT_05722 at 141.199 kb on - strand, within H281DRAFT_05722 at 141.199 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.353 kb on - strand, within H281DRAFT_05722 at 141.527 kb on - strand, within H281DRAFT_05722 at 141.791 kb on - strand at 141.830 kb on + strand at 141.880 kb on + strand at 141.880 kb on + strand at 141.880 kb on + strand at 141.881 kb on - strand at 141.884 kb on + strand at 141.884 kb on + strand at 141.884 kb on + strand at 141.884 kb on + strand at 141.885 kb on - strand at 141.885 kb on - strand at 141.885 kb on - strand at 141.907 kb on - strand at 142.276 kb on + strand, within H281DRAFT_05723 at 142.276 kb on + strand, within H281DRAFT_05723 at 142.353 kb on + strand, within H281DRAFT_05723 at 142.354 kb on - strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.837 kb on - strand, within H281DRAFT_05723
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 140,131 + +2.1 140,236 + -0.4 140,237 - +0.3 140,255 - +0.5 140,301 + -0.8 140,301 + +2.3 140,302 - +0.2 140,302 - +0.4 140,391 + +0.6 140,557 + +1.0 140,558 - -0.5 140,558 - -0.2 140,648 + H281DRAFT_05721 0.19 +0.9 140,663 + H281DRAFT_05721 0.22 -0.9 140,663 + H281DRAFT_05721 0.22 +0.2 140,664 - H281DRAFT_05721 0.22 +1.8 140,664 - H281DRAFT_05721 0.22 +0.1 140,664 - H281DRAFT_05721 0.22 -0.1 140,664 - H281DRAFT_05721 0.22 +0.4 140,667 + H281DRAFT_05721 0.23 +0.5 140,668 - H281DRAFT_05721 0.23 +0.5 140,676 - H281DRAFT_05721 0.25 +1.1 140,680 + H281DRAFT_05721 0.25 +1.7 140,680 + H281DRAFT_05721 0.25 +1.3 140,680 + H281DRAFT_05721 0.25 +0.1 140,681 - H281DRAFT_05721 0.26 -0.1 140,681 - H281DRAFT_05721 0.26 +0.7 140,797 + H281DRAFT_05721 0.49 +1.1 140,798 - H281DRAFT_05721 0.49 +0.2 140,798 - H281DRAFT_05721 0.49 +0.1 140,798 - H281DRAFT_05721 0.49 -1.4 140,837 + H281DRAFT_05721 0.57 +0.9 140,837 + H281DRAFT_05721 0.57 -1.1 140,838 - H281DRAFT_05721 0.58 +0.1 140,841 + H281DRAFT_05721 0.58 +0.0 140,912 - H281DRAFT_05721 0.73 +0.5 140,972 - H281DRAFT_05721 0.85 +1.1 140,972 - H281DRAFT_05721 0.85 +0.1 141,025 + -0.1 141,025 + -0.4 141,044 + +1.1 141,045 - -0.2 141,045 - +0.2 141,045 - -0.8 141,104 + +0.6 141,104 + +0.5 141,104 + +0.6 141,199 - H281DRAFT_05722 0.19 +0.0 141,199 - H281DRAFT_05722 0.19 +0.6 141,199 - H281DRAFT_05722 0.19 -0.7 141,295 - H281DRAFT_05722 0.31 +0.8 141,295 - H281DRAFT_05722 0.31 -1.4 141,295 - H281DRAFT_05722 0.31 +0.7 141,295 - H281DRAFT_05722 0.31 +1.7 141,295 - H281DRAFT_05722 0.31 +0.3 141,295 - H281DRAFT_05722 0.31 +0.2 141,295 - H281DRAFT_05722 0.31 +0.4 141,353 - H281DRAFT_05722 0.39 +0.4 141,527 - H281DRAFT_05722 0.61 +0.6 141,791 - +1.1 141,830 + -0.1 141,880 + -0.3 141,880 + +0.5 141,880 + +0.3 141,881 - +0.7 141,884 + -2.5 141,884 + -0.7 141,884 + +0.5 141,884 + -0.3 141,885 - +0.7 141,885 - +0.5 141,885 - -0.8 141,907 - -0.0 142,276 + H281DRAFT_05723 0.32 -0.3 142,276 + H281DRAFT_05723 0.32 +1.8 142,353 + H281DRAFT_05723 0.38 -0.2 142,354 - H281DRAFT_05723 0.38 -0.1 142,374 + H281DRAFT_05723 0.40 +0.3 142,374 + H281DRAFT_05723 0.40 +0.8 142,374 + H281DRAFT_05723 0.40 +1.4 142,375 - H281DRAFT_05723 0.40 +0.1 142,375 - H281DRAFT_05723 0.40 -0.3 142,375 - H281DRAFT_05723 0.40 -1.0 142,375 - H281DRAFT_05723 0.40 -0.1 142,375 - H281DRAFT_05723 0.40 -0.2 142,837 - H281DRAFT_05723 0.79 +0.7
Or see this region's nucleotide sequence