Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05717 and H281DRAFT_05718 are separated by 256 nucleotides H281DRAFT_05718 and H281DRAFT_05719 are separated by 228 nucleotides H281DRAFT_05719 and H281DRAFT_05720 are separated by 108 nucleotides
H281DRAFT_05717: H281DRAFT_05717 - ribulose-5-phosphate 3-epimerase, at 136,211 to 136,927
_05717
H281DRAFT_05718: H281DRAFT_05718 - ApaG protein, at 137,184 to 137,558
_05718
H281DRAFT_05719: H281DRAFT_05719 - membrane-bound lytic murein transglycosylase A, at 137,787 to 138,941
_05719
H281DRAFT_05720: H281DRAFT_05720 - phenylacetate-CoA ligase, at 139,050 to 140,354
_05720
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 136.930 kb on + strand at 137.028 kb on - strand at 137.048 kb on + strand at 137.048 kb on + strand at 137.048 kb on + strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.273 kb on + strand, within H281DRAFT_05718 at 137.273 kb on + strand, within H281DRAFT_05718 at 137.273 kb on + strand, within H281DRAFT_05718 at 137.274 kb on - strand, within H281DRAFT_05718 at 137.425 kb on - strand, within H281DRAFT_05718 at 137.426 kb on + strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.475 kb on + strand, within H281DRAFT_05718 at 137.475 kb on + strand at 137.475 kb on + strand, within H281DRAFT_05718 at 137.475 kb on + strand, within H281DRAFT_05718 at 137.475 kb on + strand, within H281DRAFT_05718 at 137.476 kb on - strand, within H281DRAFT_05718 at 137.476 kb on - strand, within H281DRAFT_05718 at 137.476 kb on - strand, within H281DRAFT_05718 at 137.592 kb on + strand at 137.593 kb on - strand at 137.801 kb on + strand at 137.816 kb on + strand at 139.051 kb on + strand at 139.169 kb on - strand at 139.169 kb on - strand at 139.197 kb on + strand, within H281DRAFT_05720 at 139.275 kb on + strand, within H281DRAFT_05720 at 139.275 kb on + strand, within H281DRAFT_05720 at 139.276 kb on - strand, within H281DRAFT_05720 at 139.285 kb on + strand, within H281DRAFT_05720 at 139.286 kb on - strand, within H281DRAFT_05720 at 139.286 kb on - strand, within H281DRAFT_05720 at 139.286 kb on - strand, within H281DRAFT_05720 at 139.397 kb on + strand at 139.398 kb on - strand, within H281DRAFT_05720 at 139.559 kb on - strand, within H281DRAFT_05720 at 139.645 kb on + strand, within H281DRAFT_05720 at 139.645 kb on + strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.728 kb on + strand, within H281DRAFT_05720 at 139.728 kb on + strand, within H281DRAFT_05720 at 139.728 kb on + strand, within H281DRAFT_05720 at 139.729 kb on - strand, within H281DRAFT_05720 at 139.729 kb on - strand, within H281DRAFT_05720 at 139.795 kb on + strand, within H281DRAFT_05720 at 139.795 kb on + strand, within H281DRAFT_05720 at 139.795 kb on + strand, within H281DRAFT_05720 at 139.796 kb on - strand, within H281DRAFT_05720 at 139.796 kb on - strand, within H281DRAFT_05720 at 139.796 kb on - strand, within H281DRAFT_05720 at 139.909 kb on + strand, within H281DRAFT_05720 at 139.910 kb on - strand, within H281DRAFT_05720
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 136,930 + -0.0 137,028 - -0.1 137,048 + -0.9 137,048 + -0.2 137,048 + +1.4 137,049 - -1.3 137,049 - -0.2 137,049 - -0.3 137,049 - -2.8 137,049 - +0.3 137,273 + H281DRAFT_05718 0.24 -0.2 137,273 + H281DRAFT_05718 0.24 -1.9 137,273 + H281DRAFT_05718 0.24 +0.4 137,274 - H281DRAFT_05718 0.24 -1.1 137,425 - H281DRAFT_05718 0.64 -1.2 137,426 + H281DRAFT_05718 0.65 -0.0 137,427 - H281DRAFT_05718 0.65 -0.0 137,427 - H281DRAFT_05718 0.65 -0.3 137,427 - H281DRAFT_05718 0.65 -0.8 137,427 - H281DRAFT_05718 0.65 -0.1 137,475 + H281DRAFT_05718 0.78 -1.7 137,475 + -1.2 137,475 + H281DRAFT_05718 0.78 +0.7 137,475 + H281DRAFT_05718 0.78 -2.6 137,475 + H281DRAFT_05718 0.78 -0.9 137,476 - H281DRAFT_05718 0.78 -1.8 137,476 - H281DRAFT_05718 0.78 -0.6 137,476 - H281DRAFT_05718 0.78 -0.4 137,592 + +0.1 137,593 - -1.9 137,801 + +0.3 137,816 + -0.0 139,051 + -0.6 139,169 - -0.6 139,169 - +0.8 139,197 + H281DRAFT_05720 0.11 -2.4 139,275 + H281DRAFT_05720 0.17 -0.6 139,275 + H281DRAFT_05720 0.17 -0.8 139,276 - H281DRAFT_05720 0.17 -0.1 139,285 + H281DRAFT_05720 0.18 -2.1 139,286 - H281DRAFT_05720 0.18 +0.2 139,286 - H281DRAFT_05720 0.18 +0.1 139,286 - H281DRAFT_05720 0.18 -0.2 139,397 + +3.9 139,398 - H281DRAFT_05720 0.27 +0.6 139,559 - H281DRAFT_05720 0.39 -1.0 139,645 + H281DRAFT_05720 0.46 +0.1 139,645 + H281DRAFT_05720 0.46 -0.1 139,646 - H281DRAFT_05720 0.46 -0.2 139,646 - H281DRAFT_05720 0.46 -0.0 139,646 - H281DRAFT_05720 0.46 -2.7 139,646 - H281DRAFT_05720 0.46 -0.5 139,646 - H281DRAFT_05720 0.46 -0.3 139,728 + H281DRAFT_05720 0.52 +0.9 139,728 + H281DRAFT_05720 0.52 -0.5 139,728 + H281DRAFT_05720 0.52 +0.4 139,729 - H281DRAFT_05720 0.52 +0.6 139,729 - H281DRAFT_05720 0.52 -0.9 139,795 + H281DRAFT_05720 0.57 -0.7 139,795 + H281DRAFT_05720 0.57 +1.0 139,795 + H281DRAFT_05720 0.57 +0.5 139,796 - H281DRAFT_05720 0.57 -0.3 139,796 - H281DRAFT_05720 0.57 +0.3 139,796 - H281DRAFT_05720 0.57 +1.1 139,909 + H281DRAFT_05720 0.66 -0.1 139,910 - H281DRAFT_05720 0.66 +2.1
Or see this region's nucleotide sequence