Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05717

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05716 and H281DRAFT_05717 are separated by 12 nucleotidesH281DRAFT_05717 and H281DRAFT_05718 are separated by 256 nucleotidesH281DRAFT_05718 and H281DRAFT_05719 are separated by 228 nucleotides H281DRAFT_05716: H281DRAFT_05716 - phosphoglycolate phosphatase, at 135,482 to 136,198 _05716 H281DRAFT_05717: H281DRAFT_05717 - ribulose-5-phosphate 3-epimerase, at 136,211 to 136,927 _05717 H281DRAFT_05718: H281DRAFT_05718 - ApaG protein, at 137,184 to 137,558 _05718 H281DRAFT_05719: H281DRAFT_05719 - membrane-bound lytic murein transglycosylase A, at 137,787 to 138,941 _05719 Position (kb) 136 137Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 135.268 kb on - strandat 135.268 kb on - strandat 135.278 kb on + strandat 135.279 kb on - strandat 135.279 kb on - strandat 135.345 kb on + strandat 135.378 kb on - strandat 135.436 kb on - strandat 135.539 kb on + strandat 135.539 kb on + strandat 135.539 kb on + strandat 135.539 kb on + strandat 135.540 kb on - strandat 135.540 kb on - strandat 135.541 kb on + strandat 135.541 kb on + strandat 135.541 kb on + strandat 135.542 kb on - strandat 135.542 kb on - strandat 135.542 kb on - strandat 135.555 kb on - strand, within H281DRAFT_05716at 135.565 kb on + strand, within H281DRAFT_05716at 135.565 kb on + strand, within H281DRAFT_05716at 135.566 kb on - strand, within H281DRAFT_05716at 135.566 kb on - strand, within H281DRAFT_05716at 135.582 kb on + strand, within H281DRAFT_05716at 135.582 kb on + strand, within H281DRAFT_05716at 135.583 kb on - strand, within H281DRAFT_05716at 135.583 kb on - strand, within H281DRAFT_05716at 135.583 kb on - strand, within H281DRAFT_05716at 135.765 kb on + strand, within H281DRAFT_05716at 135.766 kb on - strand, within H281DRAFT_05716at 135.766 kb on - strand, within H281DRAFT_05716at 135.781 kb on - strand, within H281DRAFT_05716at 135.930 kb on + strand, within H281DRAFT_05716at 135.931 kb on - strand, within H281DRAFT_05716at 135.931 kb on - strand, within H281DRAFT_05716at 136.004 kb on + strand, within H281DRAFT_05716at 136.005 kb on - strand, within H281DRAFT_05716at 136.005 kb on - strand, within H281DRAFT_05716at 136.029 kb on + strand, within H281DRAFT_05716at 136.396 kb on - strand, within H281DRAFT_05717at 136.406 kb on - strand, within H281DRAFT_05717at 136.406 kb on - strand, within H281DRAFT_05717at 136.406 kb on - strand, within H281DRAFT_05717at 136.511 kb on + strand, within H281DRAFT_05717at 136.511 kb on + strand, within H281DRAFT_05717at 136.512 kb on - strand, within H281DRAFT_05717at 136.512 kb on - strand, within H281DRAFT_05717at 136.715 kb on + strand, within H281DRAFT_05717at 136.715 kb on + strand, within H281DRAFT_05717at 136.716 kb on - strand, within H281DRAFT_05717at 136.716 kb on - strand, within H281DRAFT_05717at 136.716 kb on - strand, within H281DRAFT_05717at 136.770 kb on + strand, within H281DRAFT_05717at 136.770 kb on + strand, within H281DRAFT_05717at 136.770 kb on + strand, within H281DRAFT_05717at 136.770 kb on + strand, within H281DRAFT_05717at 136.770 kb on + strand, within H281DRAFT_05717at 136.771 kb on - strand, within H281DRAFT_05717at 136.771 kb on - strand, within H281DRAFT_05717at 136.771 kb on - strand, within H281DRAFT_05717at 136.771 kb on - strand, within H281DRAFT_05717at 136.771 kb on - strand, within H281DRAFT_05717at 136.930 kb on + strandat 137.028 kb on - strandat 137.048 kb on + strandat 137.048 kb on + strandat 137.048 kb on + strandat 137.049 kb on - strandat 137.049 kb on - strandat 137.049 kb on - strandat 137.049 kb on - strandat 137.049 kb on - strandat 137.273 kb on + strand, within H281DRAFT_05718at 137.273 kb on + strand, within H281DRAFT_05718at 137.273 kb on + strand, within H281DRAFT_05718at 137.274 kb on - strand, within H281DRAFT_05718at 137.425 kb on - strand, within H281DRAFT_05718at 137.426 kb on + strand, within H281DRAFT_05718at 137.427 kb on - strand, within H281DRAFT_05718at 137.427 kb on - strand, within H281DRAFT_05718at 137.427 kb on - strand, within H281DRAFT_05718at 137.427 kb on - strand, within H281DRAFT_05718at 137.475 kb on + strand, within H281DRAFT_05718at 137.475 kb on + strandat 137.475 kb on + strand, within H281DRAFT_05718at 137.475 kb on + strand, within H281DRAFT_05718at 137.475 kb on + strand, within H281DRAFT_05718at 137.476 kb on - strand, within H281DRAFT_05718at 137.476 kb on - strand, within H281DRAFT_05718at 137.476 kb on - strand, within H281DRAFT_05718at 137.592 kb on + strandat 137.593 kb on - strandat 137.801 kb on + strandat 137.816 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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135,268 - +0.3
135,268 - -0.3
135,278 + -1.6
135,279 - -0.3
135,279 - +0.5
135,345 + -1.5
135,378 - +0.2
135,436 - -0.3
135,539 + -0.5
135,539 + -0.2
135,539 + -0.7
135,539 + -0.3
135,540 - -0.0
135,540 - -0.0
135,541 + -4.6
135,541 + +1.0
135,541 + -0.4
135,542 - +0.2
135,542 - +1.4
135,542 - -0.6
135,555 - H281DRAFT_05716 0.10 -0.2
135,565 + H281DRAFT_05716 0.12 +1.3
135,565 + H281DRAFT_05716 0.12 +1.5
135,566 - H281DRAFT_05716 0.12 -1.1
135,566 - H281DRAFT_05716 0.12 +0.5
135,582 + H281DRAFT_05716 0.14 +0.5
135,582 + H281DRAFT_05716 0.14 +0.3
135,583 - H281DRAFT_05716 0.14 +1.3
135,583 - H281DRAFT_05716 0.14 +0.1
135,583 - H281DRAFT_05716 0.14 -1.7
135,765 + H281DRAFT_05716 0.39 -0.1
135,766 - H281DRAFT_05716 0.40 -0.4
135,766 - H281DRAFT_05716 0.40 +0.3
135,781 - H281DRAFT_05716 0.42 -0.3
135,930 + H281DRAFT_05716 0.62 +0.4
135,931 - H281DRAFT_05716 0.63 +0.9
135,931 - H281DRAFT_05716 0.63 -0.1
136,004 + H281DRAFT_05716 0.73 +0.1
136,005 - H281DRAFT_05716 0.73 +0.0
136,005 - H281DRAFT_05716 0.73 -0.5
136,029 + H281DRAFT_05716 0.76 +1.0
136,396 - H281DRAFT_05717 0.26 -0.7
136,406 - H281DRAFT_05717 0.27 -2.1
136,406 - H281DRAFT_05717 0.27 -2.4
136,406 - H281DRAFT_05717 0.27 -0.1
136,511 + H281DRAFT_05717 0.42 -1.1
136,511 + H281DRAFT_05717 0.42 -2.9
136,512 - H281DRAFT_05717 0.42 -0.5
136,512 - H281DRAFT_05717 0.42 -0.4
136,715 + H281DRAFT_05717 0.70 -1.1
136,715 + H281DRAFT_05717 0.70 -0.9
136,716 - H281DRAFT_05717 0.70 -1.1
136,716 - H281DRAFT_05717 0.70 -0.9
136,716 - H281DRAFT_05717 0.70 -1.0
136,770 + H281DRAFT_05717 0.78 -2.1
136,770 + H281DRAFT_05717 0.78 -1.4
136,770 + H281DRAFT_05717 0.78 -2.2
136,770 + H281DRAFT_05717 0.78 +0.5
136,770 + H281DRAFT_05717 0.78 -1.4
136,771 - H281DRAFT_05717 0.78 -1.3
136,771 - H281DRAFT_05717 0.78 -2.4
136,771 - H281DRAFT_05717 0.78 -0.8
136,771 - H281DRAFT_05717 0.78 -0.2
136,771 - H281DRAFT_05717 0.78 -0.1
136,930 + -0.0
137,028 - -0.1
137,048 + -0.9
137,048 + -0.2
137,048 + +1.4
137,049 - -1.3
137,049 - -0.2
137,049 - -0.3
137,049 - -2.8
137,049 - +0.3
137,273 + H281DRAFT_05718 0.24 -0.2
137,273 + H281DRAFT_05718 0.24 -1.9
137,273 + H281DRAFT_05718 0.24 +0.4
137,274 - H281DRAFT_05718 0.24 -1.1
137,425 - H281DRAFT_05718 0.64 -1.2
137,426 + H281DRAFT_05718 0.65 -0.0
137,427 - H281DRAFT_05718 0.65 -0.0
137,427 - H281DRAFT_05718 0.65 -0.3
137,427 - H281DRAFT_05718 0.65 -0.8
137,427 - H281DRAFT_05718 0.65 -0.1
137,475 + H281DRAFT_05718 0.78 -1.7
137,475 + -1.2
137,475 + H281DRAFT_05718 0.78 +0.7
137,475 + H281DRAFT_05718 0.78 -2.6
137,475 + H281DRAFT_05718 0.78 -0.9
137,476 - H281DRAFT_05718 0.78 -1.8
137,476 - H281DRAFT_05718 0.78 -0.6
137,476 - H281DRAFT_05718 0.78 -0.4
137,592 + +0.1
137,593 - -1.9
137,801 + +0.3
137,816 + -0.0

Or see this region's nucleotide sequence