Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05711 and H281DRAFT_05712 are separated by 42 nucleotides H281DRAFT_05712 and H281DRAFT_05713 are separated by 30 nucleotides H281DRAFT_05713 and H281DRAFT_05714 are separated by 17 nucleotides H281DRAFT_05714 and H281DRAFT_05715 are separated by 13 nucleotides
H281DRAFT_05711: H281DRAFT_05711 - adenylate cyclase, at 130,470 to 131,096
_05711
H281DRAFT_05712: H281DRAFT_05712 - indole-3-glycerol phosphate synthase, at 131,139 to 131,936
_05712
H281DRAFT_05713: H281DRAFT_05713 - anthranilate phosphoribosyltransferase, at 131,967 to 132,998
_05713
H281DRAFT_05714: H281DRAFT_05714 - anthranilate synthase, component II, at 133,016 to 133,609
_05714
H281DRAFT_05715: H281DRAFT_05715 - anthranilate synthase, component I, at 133,623 to 135,116
_05715
Position (kb)
131
132
133 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 130.979 kb on - strand, within H281DRAFT_05711 at 130.982 kb on + strand, within H281DRAFT_05711 at 131.121 kb on - strand at 131.121 kb on - strand at 131.121 kb on - strand at 131.121 kb on - strand at 131.514 kb on + strand, within H281DRAFT_05712 at 131.515 kb on - strand, within H281DRAFT_05712 at 131.557 kb on + strand, within H281DRAFT_05712 at 131.558 kb on - strand, within H281DRAFT_05712 at 131.623 kb on + strand, within H281DRAFT_05712 at 131.624 kb on - strand, within H281DRAFT_05712 at 131.693 kb on - strand, within H281DRAFT_05712 at 131.915 kb on + strand at 131.936 kb on + strand at 131.936 kb on + strand at 131.937 kb on - strand at 131.968 kb on + strand at 131.969 kb on - strand at 131.969 kb on - strand at 132.327 kb on - strand, within H281DRAFT_05713 at 132.331 kb on + strand, within H281DRAFT_05713 at 132.331 kb on + strand at 132.331 kb on + strand, within H281DRAFT_05713 at 132.331 kb on + strand at 132.332 kb on - strand, within H281DRAFT_05713 at 132.332 kb on - strand, within H281DRAFT_05713 at 132.332 kb on - strand, within H281DRAFT_05713 at 132.716 kb on + strand, within H281DRAFT_05713 at 132.716 kb on + strand, within H281DRAFT_05713 at 132.716 kb on + strand, within H281DRAFT_05713 at 132.726 kb on - strand, within H281DRAFT_05713 at 132.867 kb on - strand, within H281DRAFT_05713 at 133.138 kb on + strand, within H281DRAFT_05714 at 133.139 kb on - strand, within H281DRAFT_05714 at 133.525 kb on - strand, within H281DRAFT_05714 at 133.557 kb on + strand at 133.557 kb on + strand at 133.572 kb on + strand at 133.572 kb on + strand at 133.572 kb on + strand at 133.572 kb on + strand at 133.573 kb on - strand at 133.573 kb on - strand at 133.573 kb on - strand at 133.587 kb on + strand at 133.587 kb on + strand at 133.588 kb on - strand at 133.753 kb on + strand at 133.753 kb on + strand at 133.753 kb on + strand at 133.753 kb on + strand at 133.753 kb on + strand at 133.754 kb on - strand at 133.754 kb on - strand at 133.826 kb on - strand, within H281DRAFT_05715 at 133.952 kb on + strand, within H281DRAFT_05715
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 130,979 - H281DRAFT_05711 0.81 +0.4 130,982 + H281DRAFT_05711 0.82 +0.6 131,121 - +0.8 131,121 - -1.4 131,121 - -1.2 131,121 - +0.6 131,514 + H281DRAFT_05712 0.47 -0.9 131,515 - H281DRAFT_05712 0.47 -4.9 131,557 + H281DRAFT_05712 0.52 -1.7 131,558 - H281DRAFT_05712 0.53 -4.4 131,623 + H281DRAFT_05712 0.61 -4.7 131,624 - H281DRAFT_05712 0.61 -4.6 131,693 - H281DRAFT_05712 0.69 -4.6 131,915 + -2.0 131,936 + -5.7 131,936 + -4.7 131,937 - +0.5 131,968 + -1.3 131,969 - +0.8 131,969 - +0.4 132,327 - H281DRAFT_05713 0.35 -2.2 132,331 + H281DRAFT_05713 0.35 -2.8 132,331 + -1.2 132,331 + H281DRAFT_05713 0.35 -4.1 132,331 + -1.5 132,332 - H281DRAFT_05713 0.35 -2.7 132,332 - H281DRAFT_05713 0.35 -3.3 132,332 - H281DRAFT_05713 0.35 -2.7 132,716 + H281DRAFT_05713 0.73 -0.9 132,716 + H281DRAFT_05713 0.73 -3.4 132,716 + H281DRAFT_05713 0.73 -4.4 132,726 - H281DRAFT_05713 0.74 -2.2 132,867 - H281DRAFT_05713 0.87 -2.9 133,138 + H281DRAFT_05714 0.21 -1.6 133,139 - H281DRAFT_05714 0.21 +0.7 133,525 - H281DRAFT_05714 0.86 -4.7 133,557 + -1.2 133,557 + -4.2 133,572 + -3.3 133,572 + -3.6 133,572 + -4.5 133,572 + -2.7 133,573 - -5.6 133,573 - -3.2 133,573 - -5.5 133,587 + -1.7 133,587 + -1.5 133,588 - -3.1 133,753 + -4.4 133,753 + -4.1 133,753 + -2.1 133,753 + -5.4 133,753 + -3.9 133,754 - -4.0 133,754 - -1.2 133,826 - H281DRAFT_05715 0.14 -2.1 133,952 + H281DRAFT_05715 0.22 -3.8
Or see this region's nucleotide sequence