Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05671 and H281DRAFT_05672 are separated by 96 nucleotides H281DRAFT_05672 and H281DRAFT_05673 are separated by 70 nucleotides H281DRAFT_05673 and H281DRAFT_05674 are separated by 19 nucleotides H281DRAFT_05674 and H281DRAFT_05675 are separated by 368 nucleotides
H281DRAFT_05671: H281DRAFT_05671 - ubiquinol-cytochrome c reductase cytochrome c1 subunit, at 88,589 to 89,344
_05671
H281DRAFT_05672: H281DRAFT_05672 - RNA polymerase-associated protein, at 89,441 to 90,052
_05672
H281DRAFT_05673: H281DRAFT_05673 - stringent starvation protein B, at 90,123 to 90,641
_05673
H281DRAFT_05674: H281DRAFT_05674 - tRNA_Thr_TGT, at 90,661 to 90,736
_05674
H281DRAFT_05675: H281DRAFT_05675 - Methylase of polypeptide chain release factors, at 91,105 to 92,232
_05675
Position (kb)
90
91 Strain fitness (log2 ratio)
-2
-1
0
1 at 89.335 kb on + strand at 89.335 kb on + strand at 89.342 kb on + strand at 89.342 kb on + strand at 89.342 kb on + strand at 90.165 kb on + strand at 90.166 kb on - strand at 90.189 kb on + strand, within H281DRAFT_05673 at 90.189 kb on + strand, within H281DRAFT_05673 at 90.288 kb on - strand, within H281DRAFT_05673 at 90.288 kb on - strand, within H281DRAFT_05673 at 90.378 kb on - strand, within H281DRAFT_05673 at 90.390 kb on + strand, within H281DRAFT_05673 at 90.390 kb on + strand, within H281DRAFT_05673 at 90.391 kb on - strand, within H281DRAFT_05673 at 90.391 kb on - strand, within H281DRAFT_05673 at 90.391 kb on - strand, within H281DRAFT_05673 at 90.614 kb on + strand at 90.634 kb on + strand at 90.634 kb on + strand at 90.645 kb on - strand at 90.738 kb on + strand at 90.738 kb on + strand at 90.738 kb on + strand at 90.738 kb on + strand at 90.739 kb on - strand at 90.886 kb on + strand at 90.938 kb on + strand at 90.939 kb on - strand at 90.950 kb on + strand at 90.950 kb on + strand at 90.950 kb on + strand at 90.951 kb on - strand at 90.951 kb on - strand at 90.981 kb on - strand at 90.981 kb on - strand at 90.983 kb on + strand at 90.984 kb on - strand at 90.984 kb on - strand at 91.070 kb on + strand at 91.070 kb on + strand at 91.237 kb on + strand, within H281DRAFT_05675 at 91.444 kb on + strand, within H281DRAFT_05675 at 91.445 kb on - strand, within H281DRAFT_05675 at 91.498 kb on + strand, within H281DRAFT_05675 at 91.499 kb on - strand, within H281DRAFT_05675 at 91.499 kb on - strand, within H281DRAFT_05675 at 91.622 kb on - strand, within H281DRAFT_05675 at 91.622 kb on - strand, within H281DRAFT_05675
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 89,335 + +0.6 89,335 + -0.2 89,342 + -1.9 89,342 + +0.6 89,342 + -0.6 90,165 + -0.2 90,166 - -0.7 90,189 + H281DRAFT_05673 0.13 +1.0 90,189 + H281DRAFT_05673 0.13 -0.3 90,288 - H281DRAFT_05673 0.32 -0.9 90,288 - H281DRAFT_05673 0.32 +1.4 90,378 - H281DRAFT_05673 0.49 +0.6 90,390 + H281DRAFT_05673 0.51 +1.2 90,390 + H281DRAFT_05673 0.51 -0.2 90,391 - H281DRAFT_05673 0.52 +0.3 90,391 - H281DRAFT_05673 0.52 -0.1 90,391 - H281DRAFT_05673 0.52 +1.1 90,614 + +1.4 90,634 + -0.7 90,634 + -0.9 90,645 - +0.1 90,738 + +0.1 90,738 + +0.3 90,738 + -0.5 90,738 + +0.5 90,739 - -0.4 90,886 + +0.3 90,938 + -0.2 90,939 - -0.9 90,950 + -0.4 90,950 + +0.4 90,950 + -1.2 90,951 - -0.5 90,951 - -0.7 90,981 - -0.6 90,981 - +0.3 90,983 + -1.6 90,984 - -0.5 90,984 - -0.0 91,070 + +0.9 91,070 + -0.4 91,237 + H281DRAFT_05675 0.12 -0.2 91,444 + H281DRAFT_05675 0.30 -0.1 91,445 - H281DRAFT_05675 0.30 -0.2 91,498 + H281DRAFT_05675 0.35 +1.7 91,499 - H281DRAFT_05675 0.35 -0.2 91,499 - H281DRAFT_05675 0.35 +0.0 91,622 - H281DRAFT_05675 0.46 -0.5 91,622 - H281DRAFT_05675 0.46 -0.7
Or see this region's nucleotide sequence