Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05620

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05619 and H281DRAFT_05620 are separated by 294 nucleotidesH281DRAFT_05620 and H281DRAFT_05621 are separated by 128 nucleotides H281DRAFT_05619: H281DRAFT_05619 - putative transposase, at 40,245 to 40,952 _05619 H281DRAFT_05620: H281DRAFT_05620 - glutamate synthase (NADH) large subunit, at 41,247 to 45,950 _05620 H281DRAFT_05621: H281DRAFT_05621 - glutamate synthase (NADH) small subunit, at 46,079 to 47,548 _05621 Position (kb) 41 42 43 44 45 46Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3at 40.266 kb on + strandat 40.266 kb on + strandat 40.266 kb on + strandat 40.266 kb on + strandat 40.267 kb on - strandat 40.267 kb on - strandat 40.267 kb on - strandat 40.267 kb on - strandat 40.267 kb on - strandat 40.267 kb on - strandat 40.342 kb on - strand, within H281DRAFT_05619at 40.361 kb on + strand, within H281DRAFT_05619at 40.361 kb on + strand, within H281DRAFT_05619at 40.390 kb on + strand, within H281DRAFT_05619at 40.404 kb on + strand, within H281DRAFT_05619at 40.404 kb on + strand, within H281DRAFT_05619at 40.404 kb on + strand, within H281DRAFT_05619at 40.404 kb on + strand, within H281DRAFT_05619at 40.405 kb on - strand, within H281DRAFT_05619at 40.495 kb on - strand, within H281DRAFT_05619at 40.495 kb on - strand, within H281DRAFT_05619at 40.654 kb on - strand, within H281DRAFT_05619at 40.669 kb on - strand, within H281DRAFT_05619at 40.722 kb on + strand, within H281DRAFT_05619at 40.722 kb on + strand, within H281DRAFT_05619at 40.723 kb on - strand, within H281DRAFT_05619at 40.723 kb on - strand, within H281DRAFT_05619at 40.739 kb on + strand, within H281DRAFT_05619at 40.857 kb on + strand, within H281DRAFT_05619at 40.858 kb on - strand, within H281DRAFT_05619at 40.951 kb on - strandat 40.951 kb on - strandat 41.004 kb on + strandat 41.114 kb on + strandat 41.147 kb on - strandat 41.147 kb on - strandat 41.199 kb on + strandat 41.200 kb on - strandat 41.213 kb on - strandat 41.298 kb on + strandat 41.298 kb on + strandat 41.298 kb on + strandat 41.299 kb on - strandat 41.299 kb on - strandat 41.299 kb on - strandat 41.609 kb on + strandat 41.610 kb on - strandat 41.775 kb on + strand, within H281DRAFT_05620at 41.776 kb on - strand, within H281DRAFT_05620at 41.776 kb on - strand, within H281DRAFT_05620at 41.776 kb on - strand, within H281DRAFT_05620at 41.776 kb on - strand, within H281DRAFT_05620at 41.839 kb on - strand, within H281DRAFT_05620at 41.839 kb on - strand, within H281DRAFT_05620at 41.874 kb on + strand, within H281DRAFT_05620at 41.875 kb on - strand, within H281DRAFT_05620at 41.875 kb on - strandat 41.875 kb on - strand, within H281DRAFT_05620at 41.876 kb on + strand, within H281DRAFT_05620at 41.876 kb on + strand, within H281DRAFT_05620at 41.876 kb on + strand, within H281DRAFT_05620at 41.876 kb on + strand, within H281DRAFT_05620at 41.910 kb on + strand, within H281DRAFT_05620at 42.142 kb on - strand, within H281DRAFT_05620at 42.288 kb on + strand, within H281DRAFT_05620at 42.289 kb on - strand, within H281DRAFT_05620at 42.289 kb on - strand, within H281DRAFT_05620at 42.294 kb on + strand, within H281DRAFT_05620at 42.295 kb on - strand, within H281DRAFT_05620at 42.295 kb on - strand, within H281DRAFT_05620at 42.295 kb on - strand, within H281DRAFT_05620at 42.295 kb on - strand, within H281DRAFT_05620at 42.402 kb on + strand, within H281DRAFT_05620at 42.402 kb on + strand, within H281DRAFT_05620at 42.403 kb on - strand, within H281DRAFT_05620at 42.458 kb on + strand, within H281DRAFT_05620at 42.459 kb on - strand, within H281DRAFT_05620at 42.459 kb on - strand, within H281DRAFT_05620at 42.459 kb on - strand, within H281DRAFT_05620at 42.459 kb on - strand, within H281DRAFT_05620at 42.586 kb on - strand, within H281DRAFT_05620at 42.586 kb on - strand, within H281DRAFT_05620at 42.586 kb on - strand, within H281DRAFT_05620at 42.705 kb on + strand, within H281DRAFT_05620at 42.825 kb on + strand, within H281DRAFT_05620at 42.825 kb on + strand, within H281DRAFT_05620at 42.825 kb on + strand, within H281DRAFT_05620at 42.826 kb on - strand, within H281DRAFT_05620at 42.831 kb on + strand, within H281DRAFT_05620at 43.032 kb on + strand, within H281DRAFT_05620at 43.056 kb on + strand, within H281DRAFT_05620at 43.159 kb on - strand, within H281DRAFT_05620at 43.241 kb on + strand, within H281DRAFT_05620at 43.500 kb on + strand, within H281DRAFT_05620at 43.500 kb on + strand, within H281DRAFT_05620at 43.500 kb on + strand, within H281DRAFT_05620at 43.501 kb on - strand, within H281DRAFT_05620at 43.501 kb on - strand, within H281DRAFT_05620at 43.509 kb on + strand, within H281DRAFT_05620at 43.510 kb on - strand, within H281DRAFT_05620at 43.510 kb on - strand, within H281DRAFT_05620at 43.704 kb on + strand, within H281DRAFT_05620at 43.782 kb on + strand, within H281DRAFT_05620at 43.791 kb on + strand, within H281DRAFT_05620at 43.791 kb on + strand, within H281DRAFT_05620at 43.791 kb on + strand, within H281DRAFT_05620at 43.792 kb on - strand, within H281DRAFT_05620at 43.792 kb on - strand, within H281DRAFT_05620at 43.800 kb on + strand, within H281DRAFT_05620at 43.801 kb on - strand, within H281DRAFT_05620at 44.211 kb on + strand, within H281DRAFT_05620at 44.211 kb on + strand, within H281DRAFT_05620at 44.212 kb on - strand, within H281DRAFT_05620at 44.212 kb on - strand, within H281DRAFT_05620at 44.322 kb on + strandat 44.373 kb on + strand, within H281DRAFT_05620at 44.373 kb on + strand, within H281DRAFT_05620at 44.374 kb on - strand, within H281DRAFT_05620at 44.374 kb on - strand, within H281DRAFT_05620at 45.015 kb on + strand, within H281DRAFT_05620at 45.015 kb on + strand, within H281DRAFT_05620at 45.152 kb on - strand, within H281DRAFT_05620at 45.152 kb on - strand, within H281DRAFT_05620at 45.152 kb on - strand, within H281DRAFT_05620at 45.152 kb on - strand, within H281DRAFT_05620at 45.177 kb on - strand, within H281DRAFT_05620at 45.217 kb on - strand, within H281DRAFT_05620at 45.441 kb on + strand, within H281DRAFT_05620at 45.441 kb on + strand, within H281DRAFT_05620at 45.441 kb on + strand, within H281DRAFT_05620at 45.441 kb on + strand, within H281DRAFT_05620at 45.442 kb on - strand, within H281DRAFT_05620at 45.503 kb on + strandat 45.504 kb on - strandat 45.555 kb on + strandat 45.599 kb on + strandat 45.639 kb on + strandat 45.639 kb on + strandat 45.640 kb on - strandat 45.888 kb on + strandat 45.889 kb on - strandat 45.930 kb on - strandat 45.998 kb on + strandat 45.998 kb on + strandat 46.053 kb on + strandat 46.054 kb on - strandat 46.057 kb on + strandat 46.057 kb on + strandat 46.057 kb on + strandat 46.058 kb on - strandat 46.058 kb on - strandat 46.058 kb on - strandat 46.063 kb on + strandat 46.064 kb on - strandat 46.064 kb on - strandat 46.127 kb on + strandat 46.127 kb on + strandat 46.157 kb on + strandat 46.157 kb on + strandat 46.158 kb on - strandat 46.158 kb on - strandat 46.158 kb on - strandat 46.158 kb on - strandat 46.158 kb on - strandat 46.628 kb on + strand, within H281DRAFT_05621at 46.676 kb on - strand, within H281DRAFT_05621

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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40,266 + +0.8
40,266 + +0.2
40,266 + +0.6
40,266 + -0.2
40,267 - +1.0
40,267 - +0.9
40,267 - +1.2
40,267 - +1.7
40,267 - +1.0
40,267 - +0.8
40,342 - H281DRAFT_05619 0.14 +2.0
40,361 + H281DRAFT_05619 0.16 +0.4
40,361 + H281DRAFT_05619 0.16 +0.1
40,390 + H281DRAFT_05619 0.20 -0.1
40,404 + H281DRAFT_05619 0.22 +0.8
40,404 + H281DRAFT_05619 0.22 +0.8
40,404 + H281DRAFT_05619 0.22 -1.0
40,404 + H281DRAFT_05619 0.22 +0.5
40,405 - H281DRAFT_05619 0.23 +0.3
40,495 - H281DRAFT_05619 0.35 +0.9
40,495 - H281DRAFT_05619 0.35 +1.3
40,654 - H281DRAFT_05619 0.58 +0.2
40,669 - H281DRAFT_05619 0.60 +1.0
40,722 + H281DRAFT_05619 0.67 +0.1
40,722 + H281DRAFT_05619 0.67 +0.5
40,723 - H281DRAFT_05619 0.68 +1.2
40,723 - H281DRAFT_05619 0.68 +0.6
40,739 + H281DRAFT_05619 0.70 +0.3
40,857 + H281DRAFT_05619 0.86 +0.7
40,858 - H281DRAFT_05619 0.87 -1.0
40,951 - -0.0
40,951 - +0.0
41,004 + +0.3
41,114 + +0.3
41,147 - -5.6
41,147 - -0.9
41,199 + -0.6
41,200 - -4.7
41,213 - -4.0
41,298 + -1.3
41,298 + -2.4
41,298 + -5.3
41,299 - -6.6
41,299 - -3.5
41,299 - -1.7
41,609 + -3.0
41,610 - -5.5
41,775 + H281DRAFT_05620 0.11 -3.9
41,776 - H281DRAFT_05620 0.11 -2.1
41,776 - H281DRAFT_05620 0.11 -2.4
41,776 - H281DRAFT_05620 0.11 -2.5
41,776 - H281DRAFT_05620 0.11 -5.1
41,839 - H281DRAFT_05620 0.13 -4.5
41,839 - H281DRAFT_05620 0.13 -4.4
41,874 + H281DRAFT_05620 0.13 -0.9
41,875 - H281DRAFT_05620 0.13 -2.9
41,875 - -0.9
41,875 - H281DRAFT_05620 0.13 -4.1
41,876 + H281DRAFT_05620 0.13 -4.1
41,876 + H281DRAFT_05620 0.13 -3.8
41,876 + H281DRAFT_05620 0.13 -2.1
41,876 + H281DRAFT_05620 0.13 -3.5
41,910 + H281DRAFT_05620 0.14 -3.7
42,142 - H281DRAFT_05620 0.19 -2.0
42,288 + H281DRAFT_05620 0.22 -2.8
42,289 - H281DRAFT_05620 0.22 -4.1
42,289 - H281DRAFT_05620 0.22 -4.2
42,294 + H281DRAFT_05620 0.22 -2.5
42,295 - H281DRAFT_05620 0.22 -3.3
42,295 - H281DRAFT_05620 0.22 -2.7
42,295 - H281DRAFT_05620 0.22 -5.6
42,295 - H281DRAFT_05620 0.22 +3.4
42,402 + H281DRAFT_05620 0.25 -4.6
42,402 + H281DRAFT_05620 0.25 -5.3
42,403 - H281DRAFT_05620 0.25 -3.7
42,458 + H281DRAFT_05620 0.26 -4.0
42,459 - H281DRAFT_05620 0.26 -3.7
42,459 - H281DRAFT_05620 0.26 -2.8
42,459 - H281DRAFT_05620 0.26 -3.7
42,459 - H281DRAFT_05620 0.26 -3.4
42,586 - H281DRAFT_05620 0.28 -4.5
42,586 - H281DRAFT_05620 0.28 -2.5
42,586 - H281DRAFT_05620 0.28 -1.9
42,705 + H281DRAFT_05620 0.31 -5.7
42,825 + H281DRAFT_05620 0.34 -4.3
42,825 + H281DRAFT_05620 0.34 -2.6
42,825 + H281DRAFT_05620 0.34 -5.2
42,826 - H281DRAFT_05620 0.34 -2.4
42,831 + H281DRAFT_05620 0.34 -2.2
43,032 + H281DRAFT_05620 0.38 -4.7
43,056 + H281DRAFT_05620 0.38 -0.9
43,159 - H281DRAFT_05620 0.41 +0.1
43,241 + H281DRAFT_05620 0.42 -1.5
43,500 + H281DRAFT_05620 0.48 -0.5
43,500 + H281DRAFT_05620 0.48 -5.1
43,500 + H281DRAFT_05620 0.48 -3.4
43,501 - H281DRAFT_05620 0.48 -2.7
43,501 - H281DRAFT_05620 0.48 -4.6
43,509 + H281DRAFT_05620 0.48 -3.4
43,510 - H281DRAFT_05620 0.48 -2.2
43,510 - H281DRAFT_05620 0.48 -4.2
43,704 + H281DRAFT_05620 0.52 -2.1
43,782 + H281DRAFT_05620 0.54 -2.7
43,791 + H281DRAFT_05620 0.54 -2.8
43,791 + H281DRAFT_05620 0.54 -3.4
43,791 + H281DRAFT_05620 0.54 -5.6
43,792 - H281DRAFT_05620 0.54 -2.4
43,792 - H281DRAFT_05620 0.54 -1.9
43,800 + H281DRAFT_05620 0.54 -2.2
43,801 - H281DRAFT_05620 0.54 +0.1
44,211 + H281DRAFT_05620 0.63 -3.5
44,211 + H281DRAFT_05620 0.63 -4.5
44,212 - H281DRAFT_05620 0.63 -4.1
44,212 - H281DRAFT_05620 0.63 -1.5
44,322 + -0.5
44,373 + H281DRAFT_05620 0.66 -4.2
44,373 + H281DRAFT_05620 0.66 -4.2
44,374 - H281DRAFT_05620 0.66 -2.4
44,374 - H281DRAFT_05620 0.66 -3.2
45,015 + H281DRAFT_05620 0.80 -2.0
45,015 + H281DRAFT_05620 0.80 -4.7
45,152 - H281DRAFT_05620 0.83 -3.4
45,152 - H281DRAFT_05620 0.83 -3.5
45,152 - H281DRAFT_05620 0.83 -5.2
45,152 - H281DRAFT_05620 0.83 -3.7
45,177 - H281DRAFT_05620 0.84 -4.6
45,217 - H281DRAFT_05620 0.84 -5.6
45,441 + H281DRAFT_05620 0.89 -0.9
45,441 + H281DRAFT_05620 0.89 -6.2
45,441 + H281DRAFT_05620 0.89 -2.5
45,441 + H281DRAFT_05620 0.89 -4.6
45,442 - H281DRAFT_05620 0.89 -1.7
45,503 + -4.2
45,504 - -3.1
45,555 + -4.5
45,599 + -4.5
45,639 + +1.1
45,639 + -2.2
45,640 - -3.8
45,888 + +1.1
45,889 - -1.3
45,930 - -3.5
45,998 + -2.6
45,998 + -1.4
46,053 + +0.0
46,054 - -2.4
46,057 + +0.2
46,057 + +1.7
46,057 + -0.7
46,058 - -3.1
46,058 - -3.8
46,058 - -3.5
46,063 + +1.2
46,064 - +0.1
46,064 - -3.6
46,127 + -3.9
46,127 + -5.0
46,157 + -5.3
46,157 + -3.5
46,158 - -5.3
46,158 - -5.1
46,158 - -1.7
46,158 - -3.1
46,158 - -2.1
46,628 + H281DRAFT_05621 0.37 -3.9
46,676 - H281DRAFT_05621 0.41 -3.2

Or see this region's nucleotide sequence