Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05519 and H281DRAFT_05520 are separated by 427 nucleotides H281DRAFT_05520 and H281DRAFT_05521 are separated by 111 nucleotides
H281DRAFT_05519: H281DRAFT_05519 - Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, at 99,685 to 101,220
_05519
H281DRAFT_05520: H281DRAFT_05520 - transcriptional regulator, TetR family, at 101,648 to 102,304
_05520
H281DRAFT_05521: H281DRAFT_05521 - 3-hydroxyacyl-CoA dehydrogenase, at 102,416 to 103,267
_05521
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 100.778 kb on - strand, within H281DRAFT_05519 at 100.778 kb on - strand, within H281DRAFT_05519 at 100.798 kb on + strand, within H281DRAFT_05519 at 100.820 kb on + strand, within H281DRAFT_05519 at 100.821 kb on - strand, within H281DRAFT_05519 at 100.956 kb on + strand, within H281DRAFT_05519 at 101.018 kb on - strand, within H281DRAFT_05519 at 101.027 kb on + strand, within H281DRAFT_05519 at 101.049 kb on - strand, within H281DRAFT_05519 at 101.257 kb on - strand at 101.290 kb on + strand at 101.312 kb on - strand at 101.503 kb on + strand at 101.503 kb on + strand at 101.504 kb on - strand at 101.504 kb on - strand at 101.510 kb on - strand at 101.571 kb on - strand at 101.574 kb on + strand at 101.575 kb on - strand at 101.606 kb on - strand at 101.768 kb on - strand, within H281DRAFT_05520 at 101.831 kb on + strand, within H281DRAFT_05520 at 101.832 kb on - strand, within H281DRAFT_05520 at 101.832 kb on - strand, within H281DRAFT_05520 at 101.832 kb on - strand, within H281DRAFT_05520 at 101.832 kb on - strand, within H281DRAFT_05520 at 101.834 kb on + strand, within H281DRAFT_05520 at 101.834 kb on + strand, within H281DRAFT_05520 at 101.835 kb on - strand, within H281DRAFT_05520 at 101.835 kb on - strand, within H281DRAFT_05520 at 101.858 kb on + strand, within H281DRAFT_05520 at 101.882 kb on + strand, within H281DRAFT_05520 at 101.891 kb on + strand, within H281DRAFT_05520 at 101.892 kb on - strand, within H281DRAFT_05520 at 101.944 kb on - strand, within H281DRAFT_05520 at 102.263 kb on + strand at 102.263 kb on + strand at 102.263 kb on + strand at 102.264 kb on - strand at 102.359 kb on + strand at 102.429 kb on + strand at 102.540 kb on - strand, within H281DRAFT_05521 at 102.592 kb on + strand, within H281DRAFT_05521 at 102.676 kb on + strand, within H281DRAFT_05521 at 102.917 kb on - strand, within H281DRAFT_05521 at 103.172 kb on + strand, within H281DRAFT_05521 at 103.172 kb on + strand, within H281DRAFT_05521 at 103.228 kb on + strand at 103.228 kb on + strand at 103.229 kb on - strand at 103.229 kb on - strand at 103.250 kb on + strand at 103.275 kb on - strand at 103.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 100,778 - H281DRAFT_05519 0.71 +0.4 100,778 - H281DRAFT_05519 0.71 -1.2 100,798 + H281DRAFT_05519 0.72 -0.9 100,820 + H281DRAFT_05519 0.74 -0.2 100,821 - H281DRAFT_05519 0.74 +2.5 100,956 + H281DRAFT_05519 0.83 +1.0 101,018 - H281DRAFT_05519 0.87 +0.9 101,027 + H281DRAFT_05519 0.87 +0.9 101,049 - H281DRAFT_05519 0.89 +1.5 101,257 - +1.9 101,290 + +0.0 101,312 - -1.2 101,503 + +1.0 101,503 + +0.7 101,504 - +0.0 101,504 - +0.9 101,510 - +0.2 101,571 - -0.5 101,574 + +0.3 101,575 - -0.1 101,606 - -0.5 101,768 - H281DRAFT_05520 0.18 +0.1 101,831 + H281DRAFT_05520 0.28 -1.2 101,832 - H281DRAFT_05520 0.28 -0.9 101,832 - H281DRAFT_05520 0.28 -2.0 101,832 - H281DRAFT_05520 0.28 +0.6 101,832 - H281DRAFT_05520 0.28 -0.7 101,834 + H281DRAFT_05520 0.28 -0.9 101,834 + H281DRAFT_05520 0.28 -2.7 101,835 - H281DRAFT_05520 0.28 -1.4 101,835 - H281DRAFT_05520 0.28 -1.3 101,858 + H281DRAFT_05520 0.32 -2.2 101,882 + H281DRAFT_05520 0.36 +1.6 101,891 + H281DRAFT_05520 0.37 -0.6 101,892 - H281DRAFT_05520 0.37 -1.2 101,944 - H281DRAFT_05520 0.45 -1.9 102,263 + -0.9 102,263 + -0.7 102,263 + -0.2 102,264 - +0.1 102,359 + -0.4 102,429 + +0.3 102,540 - H281DRAFT_05521 0.15 +0.3 102,592 + H281DRAFT_05521 0.21 -1.0 102,676 + H281DRAFT_05521 0.31 -0.7 102,917 - H281DRAFT_05521 0.59 +3.7 103,172 + H281DRAFT_05521 0.89 +1.0 103,172 + H281DRAFT_05521 0.89 +1.0 103,228 + +0.0 103,228 + -1.0 103,229 - -0.8 103,229 - -0.6 103,250 + +0.4 103,275 - +0.9 103,300 - +0.2
Or see this region's nucleotide sequence