Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05461 and H281DRAFT_05462 are separated by 77 nucleotides H281DRAFT_05462 and H281DRAFT_05463 are separated by 102 nucleotides H281DRAFT_05463 and H281DRAFT_05464 are separated by 177 nucleotides
H281DRAFT_05461: H281DRAFT_05461 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 42,152 to 43,003
_05461
H281DRAFT_05462: H281DRAFT_05462 - hypothetical protein, at 43,081 to 43,347
_05462
H281DRAFT_05463: H281DRAFT_05463 - acetyl esterase, at 43,450 to 44,244
_05463
H281DRAFT_05464: H281DRAFT_05464 - 4-hydroxyacetophenone monooxygenase, at 44,422 to 46,401
_05464
Position (kb) 43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 42.502 kb on + strand, within H281DRAFT_05461 at 42.503 kb on - strand, within H281DRAFT_05461 at 42.537 kb on + strand, within H281DRAFT_05461 at 42.537 kb on + strand, within H281DRAFT_05461 at 42.538 kb on - strand, within H281DRAFT_05461 at 42.757 kb on - strand, within H281DRAFT_05461 at 42.969 kb on - strand at 43.005 kb on + strand at 43.006 kb on - strand at 43.006 kb on - strand at 43.006 kb on - strand at 43.218 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.261 kb on - strand, within H281DRAFT_05462 at 43.372 kb on - strand at 43.398 kb on + strand at 43.506 kb on + strand at 43.507 kb on - strand at 43.604 kb on + strand, within H281DRAFT_05463 at 43.605 kb on - strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.734 kb on - strand, within H281DRAFT_05463 at 43.734 kb on - strand, within H281DRAFT_05463 at 43.734 kb on - strand, within H281DRAFT_05463 at 43.797 kb on - strand, within H281DRAFT_05463 at 43.965 kb on + strand, within H281DRAFT_05463 at 43.966 kb on - strand, within H281DRAFT_05463 at 43.966 kb on - strand, within H281DRAFT_05463 at 44.144 kb on + strand, within H281DRAFT_05463 at 44.144 kb on + strand, within H281DRAFT_05463 at 44.144 kb on + strand, within H281DRAFT_05463 at 44.145 kb on - strand, within H281DRAFT_05463 at 44.145 kb on - strand at 44.152 kb on + strand, within H281DRAFT_05463 at 44.153 kb on - strand, within H281DRAFT_05463 at 44.380 kb on - strand at 44.380 kb on - strand at 44.380 kb on - strand at 44.384 kb on - strand at 44.384 kb on - strand at 44.384 kb on - strand at 44.391 kb on + strand at 44.392 kb on - strand at 44.529 kb on - strand at 44.618 kb on - strand at 44.720 kb on + strand, within H281DRAFT_05464 at 44.747 kb on + strand, within H281DRAFT_05464 at 44.747 kb on + strand, within H281DRAFT_05464 at 44.747 kb on + strand, within H281DRAFT_05464 at 44.748 kb on - strand, within H281DRAFT_05464 at 44.748 kb on - strand, within H281DRAFT_05464 at 44.748 kb on - strand, within H281DRAFT_05464 at 44.748 kb on - strand, within H281DRAFT_05464 at 44.886 kb on - strand, within H281DRAFT_05464 at 45.239 kb on + strand, within H281DRAFT_05464 at 45.239 kb on + strand, within H281DRAFT_05464 at 45.239 kb on + strand, within H281DRAFT_05464
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 42,502 + H281DRAFT_05461 0.41 +0.9 42,503 - H281DRAFT_05461 0.41 -0.4 42,537 + H281DRAFT_05461 0.45 +1.5 42,537 + H281DRAFT_05461 0.45 -0.1 42,538 - H281DRAFT_05461 0.45 -0.0 42,757 - H281DRAFT_05461 0.71 -1.1 42,969 - -0.1 43,005 + -0.1 43,006 - -0.2 43,006 - +0.2 43,006 - +0.1 43,218 + H281DRAFT_05462 0.51 -0.9 43,260 + H281DRAFT_05462 0.67 -0.3 43,260 + H281DRAFT_05462 0.67 -0.4 43,260 + H281DRAFT_05462 0.67 -0.1 43,260 + H281DRAFT_05462 0.67 -0.2 43,261 - H281DRAFT_05462 0.67 -2.1 43,372 - +1.1 43,398 + +0.1 43,506 + +1.1 43,507 - +0.4 43,604 + H281DRAFT_05463 0.19 -0.3 43,605 - H281DRAFT_05463 0.19 -0.7 43,733 + H281DRAFT_05463 0.36 +0.1 43,733 + H281DRAFT_05463 0.36 +0.5 43,733 + H281DRAFT_05463 0.36 +0.0 43,733 + H281DRAFT_05463 0.36 +1.8 43,734 - H281DRAFT_05463 0.36 -0.1 43,734 - H281DRAFT_05463 0.36 +1.3 43,734 - H281DRAFT_05463 0.36 -0.8 43,797 - H281DRAFT_05463 0.44 -1.8 43,965 + H281DRAFT_05463 0.65 +0.5 43,966 - H281DRAFT_05463 0.65 +0.7 43,966 - H281DRAFT_05463 0.65 -0.2 44,144 + H281DRAFT_05463 0.87 +0.1 44,144 + H281DRAFT_05463 0.87 +0.0 44,144 + H281DRAFT_05463 0.87 -0.7 44,145 - H281DRAFT_05463 0.87 -1.0 44,145 - +2.5 44,152 + H281DRAFT_05463 0.88 +0.1 44,153 - H281DRAFT_05463 0.88 +0.3 44,380 - -0.5 44,380 - -0.6 44,380 - +1.1 44,384 - +1.9 44,384 - +1.2 44,384 - -3.2 44,391 + -2.2 44,392 - +1.1 44,529 - +0.7 44,618 - -0.4 44,720 + H281DRAFT_05464 0.15 -0.5 44,747 + H281DRAFT_05464 0.16 -1.7 44,747 + H281DRAFT_05464 0.16 -0.2 44,747 + H281DRAFT_05464 0.16 +1.4 44,748 - H281DRAFT_05464 0.16 -0.1 44,748 - H281DRAFT_05464 0.16 -0.7 44,748 - H281DRAFT_05464 0.16 +0.5 44,748 - H281DRAFT_05464 0.16 -0.8 44,886 - H281DRAFT_05464 0.23 +0.6 45,239 + H281DRAFT_05464 0.41 +0.4 45,239 + H281DRAFT_05464 0.41 +0.3 45,239 + H281DRAFT_05464 0.41 -1.4
Or see this region's nucleotide sequence