Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05460 and H281DRAFT_05461 are separated by 275 nucleotides H281DRAFT_05461 and H281DRAFT_05462 are separated by 77 nucleotides H281DRAFT_05462 and H281DRAFT_05463 are separated by 102 nucleotides
H281DRAFT_05460: H281DRAFT_05460 - 3-(3-hydroxy-phenyl)propionate hydroxylase, at 40,410 to 41,876
_05460
H281DRAFT_05461: H281DRAFT_05461 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 42,152 to 43,003
_05461
H281DRAFT_05462: H281DRAFT_05462 - hypothetical protein, at 43,081 to 43,347
_05462
H281DRAFT_05463: H281DRAFT_05463 - acetyl esterase, at 43,450 to 44,244
_05463
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 41.231 kb on - strand, within H281DRAFT_05460 at 41.258 kb on - strand, within H281DRAFT_05460 at 41.269 kb on + strand, within H281DRAFT_05460 at 41.269 kb on + strand, within H281DRAFT_05460 at 41.270 kb on - strand, within H281DRAFT_05460 at 41.370 kb on + strand, within H281DRAFT_05460 at 41.370 kb on + strand, within H281DRAFT_05460 at 41.370 kb on + strand, within H281DRAFT_05460 at 41.371 kb on - strand, within H281DRAFT_05460 at 41.404 kb on + strand, within H281DRAFT_05460 at 41.404 kb on + strand, within H281DRAFT_05460 at 41.455 kb on - strand, within H281DRAFT_05460 at 41.579 kb on - strand, within H281DRAFT_05460 at 41.587 kb on + strand, within H281DRAFT_05460 at 41.588 kb on - strand, within H281DRAFT_05460 at 41.767 kb on + strand at 41.767 kb on + strand at 41.767 kb on + strand at 41.768 kb on - strand at 41.768 kb on - strand at 41.876 kb on + strand at 42.271 kb on + strand, within H281DRAFT_05461 at 42.272 kb on - strand, within H281DRAFT_05461 at 42.321 kb on + strand, within H281DRAFT_05461 at 42.322 kb on - strand, within H281DRAFT_05461 at 42.322 kb on - strand, within H281DRAFT_05461 at 42.502 kb on + strand, within H281DRAFT_05461 at 42.503 kb on - strand, within H281DRAFT_05461 at 42.537 kb on + strand, within H281DRAFT_05461 at 42.537 kb on + strand, within H281DRAFT_05461 at 42.538 kb on - strand, within H281DRAFT_05461 at 42.757 kb on - strand, within H281DRAFT_05461 at 42.969 kb on - strand at 43.005 kb on + strand at 43.006 kb on - strand at 43.006 kb on - strand at 43.006 kb on - strand at 43.218 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.260 kb on + strand, within H281DRAFT_05462 at 43.261 kb on - strand, within H281DRAFT_05462 at 43.372 kb on - strand at 43.398 kb on + strand at 43.506 kb on + strand at 43.507 kb on - strand at 43.604 kb on + strand, within H281DRAFT_05463 at 43.605 kb on - strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.733 kb on + strand, within H281DRAFT_05463 at 43.734 kb on - strand, within H281DRAFT_05463 at 43.734 kb on - strand, within H281DRAFT_05463 at 43.734 kb on - strand, within H281DRAFT_05463 at 43.797 kb on - strand, within H281DRAFT_05463 at 43.965 kb on + strand, within H281DRAFT_05463 at 43.966 kb on - strand, within H281DRAFT_05463 at 43.966 kb on - strand, within H281DRAFT_05463
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 41,231 - H281DRAFT_05460 0.56 +0.2 41,258 - H281DRAFT_05460 0.58 +0.5 41,269 + H281DRAFT_05460 0.59 +0.3 41,269 + H281DRAFT_05460 0.59 -0.7 41,270 - H281DRAFT_05460 0.59 +0.3 41,370 + H281DRAFT_05460 0.65 -0.3 41,370 + H281DRAFT_05460 0.65 -1.2 41,370 + H281DRAFT_05460 0.65 -0.7 41,371 - H281DRAFT_05460 0.66 +1.1 41,404 + H281DRAFT_05460 0.68 +0.1 41,404 + H281DRAFT_05460 0.68 +0.6 41,455 - H281DRAFT_05460 0.71 +0.1 41,579 - H281DRAFT_05460 0.80 -0.5 41,587 + H281DRAFT_05460 0.80 +1.2 41,588 - H281DRAFT_05460 0.80 +0.5 41,767 + +1.1 41,767 + -0.1 41,767 + +0.2 41,768 - -0.1 41,768 - -0.7 41,876 + -0.0 42,271 + H281DRAFT_05461 0.14 +0.1 42,272 - H281DRAFT_05461 0.14 -0.3 42,321 + H281DRAFT_05461 0.20 -0.5 42,322 - H281DRAFT_05461 0.20 +1.3 42,322 - H281DRAFT_05461 0.20 +0.2 42,502 + H281DRAFT_05461 0.41 +0.9 42,503 - H281DRAFT_05461 0.41 -0.4 42,537 + H281DRAFT_05461 0.45 +1.5 42,537 + H281DRAFT_05461 0.45 -0.1 42,538 - H281DRAFT_05461 0.45 -0.0 42,757 - H281DRAFT_05461 0.71 -1.1 42,969 - -0.1 43,005 + -0.1 43,006 - -0.2 43,006 - +0.2 43,006 - +0.1 43,218 + H281DRAFT_05462 0.51 -0.9 43,260 + H281DRAFT_05462 0.67 -0.3 43,260 + H281DRAFT_05462 0.67 -0.4 43,260 + H281DRAFT_05462 0.67 -0.1 43,260 + H281DRAFT_05462 0.67 -0.2 43,261 - H281DRAFT_05462 0.67 -2.1 43,372 - +1.1 43,398 + +0.1 43,506 + +1.1 43,507 - +0.4 43,604 + H281DRAFT_05463 0.19 -0.3 43,605 - H281DRAFT_05463 0.19 -0.7 43,733 + H281DRAFT_05463 0.36 +0.1 43,733 + H281DRAFT_05463 0.36 +0.5 43,733 + H281DRAFT_05463 0.36 +0.0 43,733 + H281DRAFT_05463 0.36 +1.8 43,734 - H281DRAFT_05463 0.36 -0.1 43,734 - H281DRAFT_05463 0.36 +1.3 43,734 - H281DRAFT_05463 0.36 -0.8 43,797 - H281DRAFT_05463 0.44 -1.8 43,965 + H281DRAFT_05463 0.65 +0.5 43,966 - H281DRAFT_05463 0.65 +0.7 43,966 - H281DRAFT_05463 0.65 -0.2
Or see this region's nucleotide sequence