Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05429 and H281DRAFT_05430 are separated by 25 nucleotides H281DRAFT_05430 and H281DRAFT_05431 overlap by 4 nucleotides H281DRAFT_05431 and H281DRAFT_05432 overlap by 8 nucleotides H281DRAFT_05432 and H281DRAFT_05433 are separated by 73 nucleotides
H281DRAFT_05429: H281DRAFT_05429 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit, at 4,703 to 6,001
_05429
H281DRAFT_05430: H281DRAFT_05430 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 6,027 to 6,734
_05430
H281DRAFT_05431: H281DRAFT_05431 - amino acid/amide ABC transporter membrane protein 2, HAAT family /amino acid/amide ABC transporter ATP-binding protein 1, HAAT family, at 6,731 to 8,593
_05431
H281DRAFT_05432: H281DRAFT_05432 - amino acid/amide ABC transporter membrane protein 1, HAAT family, at 8,586 to 9,419
_05432
H281DRAFT_05433: H281DRAFT_05433 - amino acid/amide ABC transporter substrate-binding protein, HAAT family (TC 3.A.1.4.-), at 9,493 to 10,656
_05433
Position (kb)
6
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1 at 5.982 kb on + strand at 6.001 kb on + strand at 6.069 kb on - strand at 6.340 kb on + strand, within H281DRAFT_05430 at 6.340 kb on + strand, within H281DRAFT_05430 at 6.432 kb on + strand, within H281DRAFT_05430 at 6.433 kb on - strand, within H281DRAFT_05430 at 6.691 kb on + strand at 6.691 kb on + strand at 6.692 kb on - strand at 6.793 kb on - strand at 6.802 kb on + strand at 7.217 kb on + strand, within H281DRAFT_05431 at 7.217 kb on + strand, within H281DRAFT_05431 at 7.217 kb on + strand, within H281DRAFT_05431 at 7.409 kb on + strand, within H281DRAFT_05431 at 7.409 kb on + strand, within H281DRAFT_05431 at 7.526 kb on + strand, within H281DRAFT_05431 at 7.579 kb on + strand, within H281DRAFT_05431 at 7.646 kb on + strand, within H281DRAFT_05431 at 7.646 kb on + strand, within H281DRAFT_05431 at 7.991 kb on + strand, within H281DRAFT_05431 at 8.010 kb on - strand, within H281DRAFT_05431 at 8.074 kb on - strand, within H281DRAFT_05431 at 8.375 kb on - strand, within H281DRAFT_05431 at 8.378 kb on + strand, within H281DRAFT_05431 at 8.434 kb on - strand at 8.823 kb on + strand at 8.823 kb on + strand, within H281DRAFT_05432 at 8.824 kb on - strand, within H281DRAFT_05432 at 8.979 kb on - strand, within H281DRAFT_05432 at 9.257 kb on + strand, within H281DRAFT_05432 at 9.258 kb on - strand, within H281DRAFT_05432 at 9.258 kb on - strand, within H281DRAFT_05432 at 9.289 kb on + strand, within H281DRAFT_05432 at 9.338 kb on + strand at 9.357 kb on + strand at 9.358 kb on - strand at 9.374 kb on + strand at 9.374 kb on + strand at 9.454 kb on - strand at 9.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 5,982 + +0.1 6,001 + +0.0 6,069 - +0.5 6,340 + H281DRAFT_05430 0.44 +0.1 6,340 + H281DRAFT_05430 0.44 -0.0 6,432 + H281DRAFT_05430 0.57 -0.2 6,433 - H281DRAFT_05430 0.57 -1.9 6,691 + +0.4 6,691 + +1.0 6,692 - +0.5 6,793 - -0.0 6,802 + -0.6 7,217 + H281DRAFT_05431 0.26 +0.6 7,217 + H281DRAFT_05431 0.26 -0.2 7,217 + H281DRAFT_05431 0.26 -0.3 7,409 + H281DRAFT_05431 0.36 -0.1 7,409 + H281DRAFT_05431 0.36 +0.4 7,526 + H281DRAFT_05431 0.43 -0.6 7,579 + H281DRAFT_05431 0.46 +1.0 7,646 + H281DRAFT_05431 0.49 -0.2 7,646 + H281DRAFT_05431 0.49 -0.5 7,991 + H281DRAFT_05431 0.68 -0.2 8,010 - H281DRAFT_05431 0.69 +0.8 8,074 - H281DRAFT_05431 0.72 +0.6 8,375 - H281DRAFT_05431 0.88 +0.8 8,378 + H281DRAFT_05431 0.88 +0.2 8,434 - +0.9 8,823 + +0.9 8,823 + H281DRAFT_05432 0.28 -0.3 8,824 - H281DRAFT_05432 0.29 +0.1 8,979 - H281DRAFT_05432 0.47 -1.0 9,257 + H281DRAFT_05432 0.80 -1.2 9,258 - H281DRAFT_05432 0.81 -0.6 9,258 - H281DRAFT_05432 0.81 -0.8 9,289 + H281DRAFT_05432 0.84 -0.6 9,338 + -0.4 9,357 + +0.9 9,358 - -0.3 9,374 + +0.3 9,374 + -0.2 9,454 - -0.5 9,454 - +0.4
Or see this region's nucleotide sequence