Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05428 and H281DRAFT_05429 are separated by 33 nucleotides H281DRAFT_05429 and H281DRAFT_05430 are separated by 25 nucleotides H281DRAFT_05430 and H281DRAFT_05431 overlap by 4 nucleotides
H281DRAFT_05428: H281DRAFT_05428 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 3,494 to 4,669
_05428
H281DRAFT_05429: H281DRAFT_05429 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit, at 4,703 to 6,001
_05429
H281DRAFT_05430: H281DRAFT_05430 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 6,027 to 6,734
_05430
H281DRAFT_05431: H281DRAFT_05431 - amino acid/amide ABC transporter membrane protein 2, HAAT family /amino acid/amide ABC transporter ATP-binding protein 1, HAAT family, at 6,731 to 8,593
_05431
Position (kb) 4
5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3.764 kb on + strand, within H281DRAFT_05428 at 3.774 kb on + strand, within H281DRAFT_05428 at 3.774 kb on + strand, within H281DRAFT_05428 at 3.775 kb on - strand, within H281DRAFT_05428 at 3.873 kb on + strand, within H281DRAFT_05428 at 3.873 kb on + strand, within H281DRAFT_05428 at 3.873 kb on + strand, within H281DRAFT_05428 at 3.874 kb on - strand, within H281DRAFT_05428 at 3.874 kb on - strand, within H281DRAFT_05428 at 3.990 kb on + strand, within H281DRAFT_05428 at 3.991 kb on - strand, within H281DRAFT_05428 at 4.114 kb on - strand, within H281DRAFT_05428 at 4.186 kb on - strand, within H281DRAFT_05428 at 4.186 kb on - strand, within H281DRAFT_05428 at 4.191 kb on + strand, within H281DRAFT_05428 at 4.206 kb on - strand, within H281DRAFT_05428 at 4.239 kb on + strand, within H281DRAFT_05428 at 4.284 kb on + strand, within H281DRAFT_05428 at 4.285 kb on - strand, within H281DRAFT_05428 at 4.302 kb on + strand, within H281DRAFT_05428 at 4.317 kb on + strand, within H281DRAFT_05428 at 4.329 kb on + strand, within H281DRAFT_05428 at 4.330 kb on - strand, within H281DRAFT_05428 at 4.480 kb on - strand, within H281DRAFT_05428 at 4.675 kb on - strand at 4.704 kb on + strand at 4.835 kb on + strand, within H281DRAFT_05429 at 4.879 kb on + strand, within H281DRAFT_05429 at 4.952 kb on + strand, within H281DRAFT_05429 at 4.952 kb on + strand, within H281DRAFT_05429 at 4.953 kb on - strand, within H281DRAFT_05429 at 4.953 kb on - strand, within H281DRAFT_05429 at 4.953 kb on - strand, within H281DRAFT_05429 at 5.022 kb on + strand, within H281DRAFT_05429 at 5.023 kb on - strand, within H281DRAFT_05429 at 5.100 kb on + strand, within H281DRAFT_05429 at 5.112 kb on - strand, within H281DRAFT_05429 at 5.177 kb on - strand, within H281DRAFT_05429 at 5.179 kb on + strand, within H281DRAFT_05429 at 5.179 kb on + strand, within H281DRAFT_05429 at 5.180 kb on - strand, within H281DRAFT_05429 at 5.180 kb on - strand, within H281DRAFT_05429 at 5.218 kb on + strand, within H281DRAFT_05429 at 5.219 kb on - strand, within H281DRAFT_05429 at 5.358 kb on + strand, within H281DRAFT_05429 at 5.358 kb on + strand, within H281DRAFT_05429 at 5.358 kb on + strand, within H281DRAFT_05429 at 5.396 kb on - strand, within H281DRAFT_05429 at 5.627 kb on - strand, within H281DRAFT_05429 at 5.629 kb on - strand, within H281DRAFT_05429 at 5.719 kb on - strand, within H281DRAFT_05429 at 5.982 kb on + strand at 6.001 kb on + strand at 6.069 kb on - strand at 6.340 kb on + strand, within H281DRAFT_05430 at 6.340 kb on + strand, within H281DRAFT_05430 at 6.432 kb on + strand, within H281DRAFT_05430 at 6.433 kb on - strand, within H281DRAFT_05430 at 6.691 kb on + strand at 6.691 kb on + strand at 6.692 kb on - strand at 6.793 kb on - strand at 6.802 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 3,764 + H281DRAFT_05428 0.23 -0.3 3,774 + H281DRAFT_05428 0.24 +1.2 3,774 + H281DRAFT_05428 0.24 +0.0 3,775 - H281DRAFT_05428 0.24 +0.6 3,873 + H281DRAFT_05428 0.32 -0.5 3,873 + H281DRAFT_05428 0.32 -0.4 3,873 + H281DRAFT_05428 0.32 +0.9 3,874 - H281DRAFT_05428 0.32 -1.2 3,874 - H281DRAFT_05428 0.32 -1.3 3,990 + H281DRAFT_05428 0.42 -1.5 3,991 - H281DRAFT_05428 0.42 +0.1 4,114 - H281DRAFT_05428 0.53 +0.5 4,186 - H281DRAFT_05428 0.59 -1.2 4,186 - H281DRAFT_05428 0.59 +0.3 4,191 + H281DRAFT_05428 0.59 -0.3 4,206 - H281DRAFT_05428 0.61 +1.0 4,239 + H281DRAFT_05428 0.63 +0.1 4,284 + H281DRAFT_05428 0.67 +0.7 4,285 - H281DRAFT_05428 0.67 +0.3 4,302 + H281DRAFT_05428 0.69 -1.7 4,317 + H281DRAFT_05428 0.70 +0.8 4,329 + H281DRAFT_05428 0.71 +0.4 4,330 - H281DRAFT_05428 0.71 +1.1 4,480 - H281DRAFT_05428 0.84 -1.0 4,675 - +0.1 4,704 + +0.1 4,835 + H281DRAFT_05429 0.10 -0.8 4,879 + H281DRAFT_05429 0.14 -0.2 4,952 + H281DRAFT_05429 0.19 -0.3 4,952 + H281DRAFT_05429 0.19 +0.3 4,953 - H281DRAFT_05429 0.19 -1.4 4,953 - H281DRAFT_05429 0.19 -0.3 4,953 - H281DRAFT_05429 0.19 -1.2 5,022 + H281DRAFT_05429 0.25 +0.8 5,023 - H281DRAFT_05429 0.25 +0.2 5,100 + H281DRAFT_05429 0.31 +0.9 5,112 - H281DRAFT_05429 0.31 +0.4 5,177 - H281DRAFT_05429 0.36 +0.9 5,179 + H281DRAFT_05429 0.37 -0.2 5,179 + H281DRAFT_05429 0.37 -0.0 5,180 - H281DRAFT_05429 0.37 +0.5 5,180 - H281DRAFT_05429 0.37 -0.5 5,218 + H281DRAFT_05429 0.40 +0.3 5,219 - H281DRAFT_05429 0.40 -3.5 5,358 + H281DRAFT_05429 0.50 -2.2 5,358 + H281DRAFT_05429 0.50 -0.6 5,358 + H281DRAFT_05429 0.50 +1.6 5,396 - H281DRAFT_05429 0.53 +0.4 5,627 - H281DRAFT_05429 0.71 +0.8 5,629 - H281DRAFT_05429 0.71 +0.8 5,719 - H281DRAFT_05429 0.78 -1.4 5,982 + +0.1 6,001 + +0.0 6,069 - +0.5 6,340 + H281DRAFT_05430 0.44 +0.1 6,340 + H281DRAFT_05430 0.44 -0.0 6,432 + H281DRAFT_05430 0.57 -0.2 6,433 - H281DRAFT_05430 0.57 -1.9 6,691 + +0.4 6,691 + +1.0 6,692 - +0.5 6,793 - -0.0 6,802 + -0.6
Or see this region's nucleotide sequence