Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05398 and H281DRAFT_05399 are separated by 330 nucleotides H281DRAFT_05399 and H281DRAFT_05400 are separated by 1 nucleotides
H281DRAFT_05398: H281DRAFT_05398 - hypothetical protein, at 161,142 to 161,540
_05398
H281DRAFT_05399: H281DRAFT_05399 - isoquinoline 1-oxidoreductase, alpha subunit, at 161,871 to 162,326
_05399
H281DRAFT_05400: H281DRAFT_05400 - isoquinoline 1-oxidoreductase, beta subunit, at 162,328 to 164,577
_05400
Position (kb) 161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 161.053 kb on + strand at 161.053 kb on + strand at 161.073 kb on + strand at 161.073 kb on + strand at 161.073 kb on + strand at 161.135 kb on - strand at 161.135 kb on - strand at 161.143 kb on + strand at 161.227 kb on + strand, within H281DRAFT_05398 at 161.478 kb on - strand, within H281DRAFT_05398 at 161.654 kb on + strand at 161.654 kb on + strand at 161.655 kb on - strand at 161.885 kb on + strand at 161.904 kb on - strand at 162.046 kb on + strand at 162.279 kb on + strand, within H281DRAFT_05399 at 162.280 kb on - strand, within H281DRAFT_05399 at 162.280 kb on - strand at 162.601 kb on - strand, within H281DRAFT_05400 at 162.784 kb on + strand, within H281DRAFT_05400 at 163.233 kb on + strand, within H281DRAFT_05400 at 163.234 kb on - strand, within H281DRAFT_05400 at 163.234 kb on - strand, within H281DRAFT_05400
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 161,053 + +0.1 161,053 + -1.0 161,073 + +0.2 161,073 + +0.0 161,073 + +0.9 161,135 - +0.3 161,135 - +0.9 161,143 + -0.9 161,227 + H281DRAFT_05398 0.21 +0.3 161,478 - H281DRAFT_05398 0.84 -0.3 161,654 + +0.1 161,654 + +1.0 161,655 - +0.1 161,885 + -0.1 161,904 - +0.6 162,046 + +0.7 162,279 + H281DRAFT_05399 0.89 +0.3 162,280 - H281DRAFT_05399 0.90 +1.5 162,280 - +1.7 162,601 - H281DRAFT_05400 0.12 -3.4 162,784 + H281DRAFT_05400 0.20 +1.5 163,233 + H281DRAFT_05400 0.40 +0.8 163,234 - H281DRAFT_05400 0.40 +0.1 163,234 - H281DRAFT_05400 0.40 -0.5
Or see this region's nucleotide sequence