Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05219 and H281DRAFT_05220 are separated by 66 nucleotides H281DRAFT_05220 and H281DRAFT_05221 are separated by 406 nucleotides H281DRAFT_05221 and H281DRAFT_05222 are separated by 102 nucleotides H281DRAFT_05222 and H281DRAFT_05223 overlap by 4 nucleotides
H281DRAFT_05219: H281DRAFT_05219 - transcriptional regulator, LysR family, at 180,758 to 181,642
_05219
H281DRAFT_05220: H281DRAFT_05220 - transcriptional regulator, AsnC family, at 181,709 to 182,167
_05220
H281DRAFT_05221: H281DRAFT_05221 - Saccharopine dehydrogenase, NADP-dependent, at 182,574 to 183,680
_05221
H281DRAFT_05222: H281DRAFT_05222 - Dephospho-CoA kinase, at 183,783 to 184,403
_05222
H281DRAFT_05223: H281DRAFT_05223 - hypothetical protein, at 184,400 to 184,699
_05223
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1
2 at 181.922 kb on + strand, within H281DRAFT_05220 at 181.922 kb on + strand, within H281DRAFT_05220 at 181.928 kb on + strand, within H281DRAFT_05220 at 181.928 kb on + strand, within H281DRAFT_05220 at 182.008 kb on - strand, within H281DRAFT_05220 at 182.034 kb on - strand, within H281DRAFT_05220 at 182.120 kb on - strand, within H281DRAFT_05220 at 182.484 kb on - strand at 182.532 kb on + strand at 182.532 kb on + strand at 182.574 kb on - strand at 182.574 kb on - strand at 182.845 kb on - strand, within H281DRAFT_05221 at 183.090 kb on + strand, within H281DRAFT_05221 at 183.259 kb on - strand, within H281DRAFT_05221 at 183.259 kb on - strand, within H281DRAFT_05221 at 183.268 kb on + strand at 183.633 kb on + strand at 183.941 kb on - strand, within H281DRAFT_05222 at 184.266 kb on - strand, within H281DRAFT_05222 at 184.268 kb on - strand, within H281DRAFT_05222 at 184.268 kb on - strand, within H281DRAFT_05222 at 184.268 kb on - strand, within H281DRAFT_05222 at 184.276 kb on + strand, within H281DRAFT_05222 at 184.385 kb on + strand at 184.386 kb on - strand at 184.462 kb on + strand, within H281DRAFT_05223 at 184.462 kb on + strand, within H281DRAFT_05223 at 184.462 kb on + strand, within H281DRAFT_05223 at 184.652 kb on - strand, within H281DRAFT_05223 at 184.652 kb on - strand, within H281DRAFT_05223
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 181,922 + H281DRAFT_05220 0.46 +1.0 181,922 + H281DRAFT_05220 0.46 +0.2 181,928 + H281DRAFT_05220 0.48 +0.0 181,928 + H281DRAFT_05220 0.48 -0.1 182,008 - H281DRAFT_05220 0.65 +2.1 182,034 - H281DRAFT_05220 0.71 -0.3 182,120 - H281DRAFT_05220 0.90 +1.0 182,484 - -0.8 182,532 + -1.2 182,532 + +0.7 182,574 - -0.6 182,574 - +0.7 182,845 - H281DRAFT_05221 0.24 +1.3 183,090 + H281DRAFT_05221 0.47 +0.9 183,259 - H281DRAFT_05221 0.62 +1.5 183,259 - H281DRAFT_05221 0.62 -1.0 183,268 + -0.2 183,633 + -0.1 183,941 - H281DRAFT_05222 0.25 -0.4 184,266 - H281DRAFT_05222 0.78 +0.2 184,268 - H281DRAFT_05222 0.78 +0.2 184,268 - H281DRAFT_05222 0.78 +0.2 184,268 - H281DRAFT_05222 0.78 -0.7 184,276 + H281DRAFT_05222 0.79 +0.0 184,385 + +0.2 184,386 - -1.0 184,462 + H281DRAFT_05223 0.21 +0.1 184,462 + H281DRAFT_05223 0.21 +1.9 184,462 + H281DRAFT_05223 0.21 +0.2 184,652 - H281DRAFT_05223 0.84 +0.1 184,652 - H281DRAFT_05223 0.84 -0.2
Or see this region's nucleotide sequence