Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05160 and H281DRAFT_05161 overlap by 4 nucleotides H281DRAFT_05161 and H281DRAFT_05162 are separated by 368 nucleotides H281DRAFT_05162 and H281DRAFT_05163 are separated by 184 nucleotides
H281DRAFT_05160: H281DRAFT_05160 - allantoicase, at 113,631 to 114,644
_05160
H281DRAFT_05161: H281DRAFT_05161 - ureidoglycolate lyase, at 114,641 to 115,153
_05161
H281DRAFT_05162: H281DRAFT_05162 - Uncharacterized membrane protein, at 115,522 to 116,715
_05162
H281DRAFT_05163: H281DRAFT_05163 - 5-hydroxyisourate hydrolase, at 116,900 to 117,253
_05163
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 114.535 kb on - strand, within H281DRAFT_05160 at 115.275 kb on - strand at 115.275 kb on - strand at 115.280 kb on + strand at 115.426 kb on + strand at 115.427 kb on - strand at 115.492 kb on - strand at 115.631 kb on + strand at 115.632 kb on - strand at 115.668 kb on + strand, within H281DRAFT_05162 at 115.669 kb on - strand, within H281DRAFT_05162 at 115.871 kb on + strand, within H281DRAFT_05162 at 115.871 kb on + strand, within H281DRAFT_05162 at 115.872 kb on - strand, within H281DRAFT_05162 at 115.961 kb on + strand, within H281DRAFT_05162 at 115.962 kb on - strand, within H281DRAFT_05162 at 115.973 kb on + strand, within H281DRAFT_05162 at 115.974 kb on - strand, within H281DRAFT_05162 at 115.974 kb on - strand, within H281DRAFT_05162 at 116.027 kb on + strand, within H281DRAFT_05162 at 116.066 kb on + strand, within H281DRAFT_05162 at 116.187 kb on - strand, within H281DRAFT_05162 at 116.263 kb on + strand, within H281DRAFT_05162 at 116.263 kb on + strand, within H281DRAFT_05162 at 116.264 kb on - strand, within H281DRAFT_05162 at 116.303 kb on + strand, within H281DRAFT_05162 at 116.391 kb on - strand, within H281DRAFT_05162 at 116.510 kb on + strand, within H281DRAFT_05162 at 116.511 kb on - strand, within H281DRAFT_05162 at 116.511 kb on - strand, within H281DRAFT_05162 at 116.511 kb on - strand, within H281DRAFT_05162 at 116.525 kb on + strand, within H281DRAFT_05162 at 116.543 kb on - strand, within H281DRAFT_05162 at 116.628 kb on - strand at 117.305 kb on + strand at 117.342 kb on - strand at 117.387 kb on - strand at 117.387 kb on - strand at 117.387 kb on - strand at 117.429 kb on + strand at 117.430 kb on - strand at 117.430 kb on - strand at 117.497 kb on + strand at 117.497 kb on + strand at 117.539 kb on + strand at 117.539 kb on + strand at 117.539 kb on + strand at 117.540 kb on - strand at 117.680 kb on + strand at 117.680 kb on + strand at 117.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 114,535 - H281DRAFT_05160 0.89 -2.4 115,275 - -0.1 115,275 - +1.1 115,280 + +0.1 115,426 + -0.1 115,427 - -0.1 115,492 - +1.3 115,631 + -2.0 115,632 - -1.2 115,668 + H281DRAFT_05162 0.12 -1.2 115,669 - H281DRAFT_05162 0.12 -0.4 115,871 + H281DRAFT_05162 0.29 -1.1 115,871 + H281DRAFT_05162 0.29 -2.2 115,872 - H281DRAFT_05162 0.29 -1.4 115,961 + H281DRAFT_05162 0.37 -0.8 115,962 - H281DRAFT_05162 0.37 -0.8 115,973 + H281DRAFT_05162 0.38 -1.1 115,974 - H281DRAFT_05162 0.38 -2.6 115,974 - H281DRAFT_05162 0.38 -1.7 116,027 + H281DRAFT_05162 0.42 -2.5 116,066 + H281DRAFT_05162 0.46 -1.9 116,187 - H281DRAFT_05162 0.56 -3.4 116,263 + H281DRAFT_05162 0.62 -2.7 116,263 + H281DRAFT_05162 0.62 -1.2 116,264 - H281DRAFT_05162 0.62 -0.1 116,303 + H281DRAFT_05162 0.65 -4.4 116,391 - H281DRAFT_05162 0.73 -2.4 116,510 + H281DRAFT_05162 0.83 -3.2 116,511 - H281DRAFT_05162 0.83 -0.2 116,511 - H281DRAFT_05162 0.83 -3.4 116,511 - H281DRAFT_05162 0.83 -2.0 116,525 + H281DRAFT_05162 0.84 -2.3 116,543 - H281DRAFT_05162 0.86 -0.6 116,628 - -1.2 117,305 + -1.7 117,342 - +0.1 117,387 - +0.1 117,387 - -0.6 117,387 - -1.3 117,429 + -0.7 117,430 - +0.2 117,430 - -0.6 117,497 + +1.0 117,497 + +1.7 117,539 + +1.0 117,539 + +2.0 117,539 + +1.3 117,540 - +0.1 117,680 + -1.2 117,680 + +1.0 117,681 - -0.6
Or see this region's nucleotide sequence