Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05122 and H281DRAFT_05123 are separated by 630 nucleotides H281DRAFT_05123 and H281DRAFT_05124 are separated by 14 nucleotides H281DRAFT_05124 and H281DRAFT_05125 overlap by 4 nucleotides
H281DRAFT_05122: H281DRAFT_05122 - host factor-I protein, at 71,705 to 72,172
_05122
H281DRAFT_05123: H281DRAFT_05123 - hypothetical protein, at 72,803 to 72,940
_05123
H281DRAFT_05124: H281DRAFT_05124 - RNA polymerase sigma-70 factor, ECF subfamily, at 72,955 to 73,482
_05124
H281DRAFT_05125: H281DRAFT_05125 - Transmembrane transcriptional regulator (anti-sigma factor RsiW), at 73,479 to 74,342
_05125
Position (kb) 72
73 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 71.850 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.004 kb on - strand, within H281DRAFT_05122 at 72.004 kb on - strand, within H281DRAFT_05122 at 72.096 kb on + strand, within H281DRAFT_05122 at 72.186 kb on + strand at 72.259 kb on + strand at 72.260 kb on - strand at 72.260 kb on - strand at 72.279 kb on + strand at 72.279 kb on + strand at 72.280 kb on - strand at 72.291 kb on - strand at 72.295 kb on - strand at 72.413 kb on + strand at 72.414 kb on - strand at 72.414 kb on - strand at 72.451 kb on + strand at 72.451 kb on + strand at 72.532 kb on - strand at 72.581 kb on - strand at 72.727 kb on + strand at 72.727 kb on + strand at 72.727 kb on + strand at 72.728 kb on - strand at 72.761 kb on + strand at 72.761 kb on + strand at 72.777 kb on - strand at 72.777 kb on - strand at 72.777 kb on - strand at 72.802 kb on + strand at 72.834 kb on + strand, within H281DRAFT_05123 at 72.922 kb on + strand, within H281DRAFT_05123 at 73.105 kb on - strand, within H281DRAFT_05124 at 73.144 kb on + strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.270 kb on + strand, within H281DRAFT_05124 at 73.270 kb on + strand, within H281DRAFT_05124 at 73.270 kb on + strand, within H281DRAFT_05124 at 73.271 kb on - strand, within H281DRAFT_05124 at 73.446 kb on + strand at 73.657 kb on + strand, within H281DRAFT_05125 at 73.658 kb on - strand, within H281DRAFT_05125 at 73.928 kb on - strand, within H281DRAFT_05125 at 73.933 kb on - strand, within H281DRAFT_05125
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 71,850 + H281DRAFT_05122 0.31 +0.3 72,003 + H281DRAFT_05122 0.64 +1.1 72,003 + H281DRAFT_05122 0.64 +0.7 72,003 + H281DRAFT_05122 0.64 +0.2 72,003 + H281DRAFT_05122 0.64 +0.9 72,004 - H281DRAFT_05122 0.64 +1.3 72,004 - H281DRAFT_05122 0.64 +0.5 72,096 + H281DRAFT_05122 0.84 +1.4 72,186 + +1.6 72,259 + +0.8 72,260 - +0.2 72,260 - -0.2 72,279 + +0.8 72,279 + +0.2 72,280 - +0.1 72,291 - -0.3 72,295 - +0.8 72,413 + +0.2 72,414 - +0.5 72,414 - -0.4 72,451 + +0.8 72,451 + +0.2 72,532 - -0.3 72,581 - +0.7 72,727 + +0.7 72,727 + -0.1 72,727 + -3.5 72,728 - -0.1 72,761 + +0.6 72,761 + +0.7 72,777 - +0.9 72,777 - +0.4 72,777 - -0.1 72,802 + -0.2 72,834 + H281DRAFT_05123 0.22 +1.1 72,922 + H281DRAFT_05123 0.86 -0.8 73,105 - H281DRAFT_05124 0.28 -0.8 73,144 + H281DRAFT_05124 0.36 +0.6 73,145 - H281DRAFT_05124 0.36 -2.0 73,145 - H281DRAFT_05124 0.36 -1.5 73,145 - H281DRAFT_05124 0.36 +0.1 73,145 - H281DRAFT_05124 0.36 -0.2 73,270 + H281DRAFT_05124 0.60 -1.0 73,270 + H281DRAFT_05124 0.60 -0.4 73,270 + H281DRAFT_05124 0.60 -0.9 73,271 - H281DRAFT_05124 0.60 +0.8 73,446 + -0.1 73,657 + H281DRAFT_05125 0.21 +2.0 73,658 - H281DRAFT_05125 0.21 -1.0 73,928 - H281DRAFT_05125 0.52 +0.4 73,933 - H281DRAFT_05125 0.53 -1.2
Or see this region's nucleotide sequence