Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05119 and H281DRAFT_05120 are separated by 156 nucleotides H281DRAFT_05120 and H281DRAFT_05121 are separated by 327 nucleotides H281DRAFT_05121 and H281DRAFT_05122 are separated by 29 nucleotides H281DRAFT_05122 and H281DRAFT_05123 are separated by 630 nucleotides H281DRAFT_05123 and H281DRAFT_05124 are separated by 14 nucleotides
H281DRAFT_05119: H281DRAFT_05119 - Protein of unknown function (DUF2968), at 70,032 to 70,772
_05119
H281DRAFT_05120: H281DRAFT_05120 - hypothetical protein, at 70,929 to 71,123
_05120
H281DRAFT_05121: H281DRAFT_05121 - hypothetical protein, at 71,451 to 71,675
_05121
H281DRAFT_05122: H281DRAFT_05122 - host factor-I protein, at 71,705 to 72,172
_05122
H281DRAFT_05123: H281DRAFT_05123 - hypothetical protein, at 72,803 to 72,940
_05123
H281DRAFT_05124: H281DRAFT_05124 - RNA polymerase sigma-70 factor, ECF subfamily, at 72,955 to 73,482
_05124
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 70.899 kb on + strand at 70.900 kb on - strand at 71.052 kb on - strand at 71.197 kb on + strand at 71.200 kb on - strand at 71.200 kb on - strand at 71.636 kb on - strand, within H281DRAFT_05121 at 71.707 kb on - strand at 71.850 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.003 kb on + strand, within H281DRAFT_05122 at 72.004 kb on - strand, within H281DRAFT_05122 at 72.004 kb on - strand, within H281DRAFT_05122 at 72.096 kb on + strand, within H281DRAFT_05122 at 72.186 kb on + strand at 72.259 kb on + strand at 72.260 kb on - strand at 72.260 kb on - strand at 72.279 kb on + strand at 72.279 kb on + strand at 72.280 kb on - strand at 72.291 kb on - strand at 72.295 kb on - strand at 72.413 kb on + strand at 72.414 kb on - strand at 72.414 kb on - strand at 72.451 kb on + strand at 72.451 kb on + strand at 72.532 kb on - strand at 72.581 kb on - strand at 72.727 kb on + strand at 72.727 kb on + strand at 72.727 kb on + strand at 72.728 kb on - strand at 72.761 kb on + strand at 72.761 kb on + strand at 72.777 kb on - strand at 72.777 kb on - strand at 72.777 kb on - strand at 72.802 kb on + strand at 72.834 kb on + strand, within H281DRAFT_05123 at 72.922 kb on + strand, within H281DRAFT_05123 at 73.105 kb on - strand, within H281DRAFT_05124 at 73.144 kb on + strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124 at 73.145 kb on - strand, within H281DRAFT_05124
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 70,899 + +0.4 70,900 - -0.2 71,052 - +1.2 71,197 + +0.1 71,200 - -0.0 71,200 - -0.8 71,636 - H281DRAFT_05121 0.82 -0.4 71,707 - +0.9 71,850 + H281DRAFT_05122 0.31 +0.3 72,003 + H281DRAFT_05122 0.64 +1.1 72,003 + H281DRAFT_05122 0.64 +0.7 72,003 + H281DRAFT_05122 0.64 +0.2 72,003 + H281DRAFT_05122 0.64 +0.9 72,004 - H281DRAFT_05122 0.64 +1.3 72,004 - H281DRAFT_05122 0.64 +0.5 72,096 + H281DRAFT_05122 0.84 +1.4 72,186 + +1.6 72,259 + +0.8 72,260 - +0.2 72,260 - -0.2 72,279 + +0.8 72,279 + +0.2 72,280 - +0.1 72,291 - -0.3 72,295 - +0.8 72,413 + +0.2 72,414 - +0.5 72,414 - -0.4 72,451 + +0.8 72,451 + +0.2 72,532 - -0.3 72,581 - +0.7 72,727 + +0.7 72,727 + -0.1 72,727 + -3.5 72,728 - -0.1 72,761 + +0.6 72,761 + +0.7 72,777 - +0.9 72,777 - +0.4 72,777 - -0.1 72,802 + -0.2 72,834 + H281DRAFT_05123 0.22 +1.1 72,922 + H281DRAFT_05123 0.86 -0.8 73,105 - H281DRAFT_05124 0.28 -0.8 73,144 + H281DRAFT_05124 0.36 +0.6 73,145 - H281DRAFT_05124 0.36 -2.0 73,145 - H281DRAFT_05124 0.36 -1.5 73,145 - H281DRAFT_05124 0.36 +0.1 73,145 - H281DRAFT_05124 0.36 -0.2
Or see this region's nucleotide sequence