Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05084 and H281DRAFT_05085 are separated by 225 nucleotides H281DRAFT_05085 and H281DRAFT_05086 overlap by 4 nucleotides
H281DRAFT_05084: H281DRAFT_05084 - Uncharacterized membrane protein YccC, at 20,886 to 23,381
_05084
H281DRAFT_05085: H281DRAFT_05085 - DNA helicase/exodeoxyribonuclease V, gamma subunit, at 23,607 to 26,984
_05085
H281DRAFT_05086: H281DRAFT_05086 - DNA helicase/exodeoxyribonuclease V, beta subunit, at 26,981 to 30,682
_05086
Position (kb) 23
24
25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 22.644 kb on - strand, within H281DRAFT_05084 at 22.784 kb on - strand, within H281DRAFT_05084 at 22.813 kb on + strand, within H281DRAFT_05084 at 22.837 kb on + strand, within H281DRAFT_05084 at 22.921 kb on + strand, within H281DRAFT_05084 at 22.922 kb on - strand, within H281DRAFT_05084 at 22.927 kb on + strand, within H281DRAFT_05084 at 23.192 kb on - strand at 23.210 kb on + strand at 23.332 kb on + strand at 23.338 kb on + strand at 23.347 kb on + strand at 24.019 kb on - strand, within H281DRAFT_05085 at 24.028 kb on - strand, within H281DRAFT_05085 at 24.468 kb on + strand, within H281DRAFT_05085 at 24.531 kb on - strand, within H281DRAFT_05085 at 24.531 kb on - strand, within H281DRAFT_05085 at 26.245 kb on + strand, within H281DRAFT_05085 at 26.586 kb on - strand, within H281DRAFT_05085 at 27.030 kb on + strand at 27.056 kb on - strand at 27.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 22,644 - H281DRAFT_05084 0.70 -0.2 22,784 - H281DRAFT_05084 0.76 +0.8 22,813 + H281DRAFT_05084 0.77 +1.4 22,837 + H281DRAFT_05084 0.78 -0.0 22,921 + H281DRAFT_05084 0.82 +0.5 22,922 - H281DRAFT_05084 0.82 +0.8 22,927 + H281DRAFT_05084 0.82 -0.1 23,192 - -0.2 23,210 + +1.6 23,332 + -0.3 23,338 + -0.0 23,347 + +0.1 24,019 - H281DRAFT_05085 0.12 -1.8 24,028 - H281DRAFT_05085 0.12 -0.1 24,468 + H281DRAFT_05085 0.25 +0.7 24,531 - H281DRAFT_05085 0.27 -1.0 24,531 - H281DRAFT_05085 0.27 -1.5 26,245 + H281DRAFT_05085 0.78 -2.4 26,586 - H281DRAFT_05085 0.88 -3.0 27,030 + -0.0 27,056 - -2.2 27,111 - -1.8
Or see this region's nucleotide sequence