Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05005

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05003 and H281DRAFT_05004 overlap by 4 nucleotidesH281DRAFT_05004 and H281DRAFT_05005 are separated by 54 nucleotidesH281DRAFT_05005 and H281DRAFT_05006 are separated by 311 nucleotides H281DRAFT_05003: H281DRAFT_05003 - type III secretion apparatus lipoprotein, YscJ/HrcJ family, at 188,849 to 189,670 _05003 H281DRAFT_05004: H281DRAFT_05004 - type III secretion inner rod protein HrpB2, at 189,667 to 190,059 _05004 H281DRAFT_05005: H281DRAFT_05005 - type III secretion protein HrpB1, at 190,114 to 190,731 _05005 H281DRAFT_05006: H281DRAFT_05006 - type III secretion protein U, at 191,043 to 192,119 _05006 Position (kb) 190 191Strain fitness (log2 ratio) -1 0 1at 190.585 kb on - strand, within H281DRAFT_05005at 190.673 kb on + strandat 190.674 kb on - strandat 191.255 kb on + strand, within H281DRAFT_05006at 191.492 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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190,585 - H281DRAFT_05005 0.76 +0.8
190,673 + +0.8
190,674 - -0.7
191,255 + H281DRAFT_05006 0.20 -0.2
191,492 - +0.8

Or see this region's nucleotide sequence