Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04634 and H281DRAFT_04635 are separated by 60 nucleotides H281DRAFT_04635 and H281DRAFT_04636 are separated by 9 nucleotides H281DRAFT_04636 and H281DRAFT_04637 are separated by 221 nucleotides
H281DRAFT_04634: H281DRAFT_04634 - ATP-binding cassette, subfamily B, at 32,255 to 34,144
_04634
H281DRAFT_04635: H281DRAFT_04635 - Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon), at 34,205 to 34,912
_04635
H281DRAFT_04636: H281DRAFT_04636 - hypothetical protein, at 34,922 to 36,034
_04636
H281DRAFT_04637: H281DRAFT_04637 - LPS-assembly protein, at 36,256 to 38,622
_04637
Position (kb)
34
35
36
37 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 34.278 kb on + strand at 34.278 kb on + strand, within H281DRAFT_04635 at 34.323 kb on + strand, within H281DRAFT_04635 at 34.932 kb on + strand at 35.108 kb on - strand, within H281DRAFT_04636 at 35.108 kb on - strand, within H281DRAFT_04636 at 35.143 kb on - strand, within H281DRAFT_04636 at 35.200 kb on - strand, within H281DRAFT_04636 at 35.202 kb on + strand, within H281DRAFT_04636 at 35.202 kb on + strand, within H281DRAFT_04636 at 35.203 kb on - strand, within H281DRAFT_04636 at 35.274 kb on + strand, within H281DRAFT_04636 at 35.274 kb on + strand, within H281DRAFT_04636 at 35.274 kb on + strand, within H281DRAFT_04636 at 35.275 kb on - strand, within H281DRAFT_04636 at 35.275 kb on - strand, within H281DRAFT_04636 at 35.275 kb on - strand, within H281DRAFT_04636 at 35.279 kb on - strand, within H281DRAFT_04636 at 35.279 kb on - strand, within H281DRAFT_04636 at 35.279 kb on - strand, within H281DRAFT_04636 at 35.289 kb on + strand, within H281DRAFT_04636 at 35.312 kb on + strand, within H281DRAFT_04636 at 35.683 kb on - strand, within H281DRAFT_04636 at 35.683 kb on - strand, within H281DRAFT_04636 at 35.883 kb on + strand, within H281DRAFT_04636 at 36.074 kb on + strand at 36.074 kb on + strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.079 kb on + strand at 36.079 kb on + strand at 36.163 kb on + strand at 36.163 kb on + strand at 36.163 kb on + strand at 36.163 kb on + strand at 36.253 kb on + strand at 36.253 kb on + strand at 36.253 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 34,278 + +3.0 34,278 + H281DRAFT_04635 0.10 -2.4 34,323 + H281DRAFT_04635 0.17 -2.4 34,932 + +0.0 35,108 - H281DRAFT_04636 0.17 -0.1 35,108 - H281DRAFT_04636 0.17 -0.0 35,143 - H281DRAFT_04636 0.20 -2.3 35,200 - H281DRAFT_04636 0.25 +0.3 35,202 + H281DRAFT_04636 0.25 -0.7 35,202 + H281DRAFT_04636 0.25 +1.3 35,203 - H281DRAFT_04636 0.25 -0.1 35,274 + H281DRAFT_04636 0.32 +0.4 35,274 + H281DRAFT_04636 0.32 +1.1 35,274 + H281DRAFT_04636 0.32 -0.8 35,275 - H281DRAFT_04636 0.32 +0.3 35,275 - H281DRAFT_04636 0.32 +0.9 35,275 - H281DRAFT_04636 0.32 +0.0 35,279 - H281DRAFT_04636 0.32 -0.5 35,279 - H281DRAFT_04636 0.32 -1.1 35,279 - H281DRAFT_04636 0.32 +0.5 35,289 + H281DRAFT_04636 0.33 +0.3 35,312 + H281DRAFT_04636 0.35 +1.0 35,683 - H281DRAFT_04636 0.68 +0.3 35,683 - H281DRAFT_04636 0.68 -0.2 35,883 + H281DRAFT_04636 0.86 -0.8 36,074 + +1.2 36,074 + +0.1 36,075 - -0.2 36,075 - -3.8 36,075 - +1.0 36,075 - +0.7 36,075 - -0.2 36,076 + +0.5 36,076 + +0.6 36,076 + +0.3 36,076 + -0.7 36,076 + +1.6 36,079 + -0.2 36,079 + -1.1 36,163 + -0.1 36,163 + -0.3 36,163 + -0.4 36,163 + +1.4 36,253 + -0.3 36,253 + -0.6 36,253 + +0.3
Or see this region's nucleotide sequence