Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04630 and H281DRAFT_04631 are separated by 76 nucleotides H281DRAFT_04631 and H281DRAFT_04632 are separated by 158 nucleotides H281DRAFT_04632 and H281DRAFT_04633 are separated by 102 nucleotides H281DRAFT_04633 and H281DRAFT_04634 are separated by 309 nucleotides
H281DRAFT_04630: H281DRAFT_04630 - PAS domain S-box-containing protein/diguanylate cyclase (GGDEF) domain-containing protein, at 26,567 to 29,677
_04630
H281DRAFT_04631: H281DRAFT_04631 - Glutathione S-transferase, at 29,754 to 30,368
_04631
H281DRAFT_04632: H281DRAFT_04632 - LysR family transcriptional regulator, glycine cleavage system transcriptional activator, at 30,527 to 31,447
_04632
H281DRAFT_04633: H281DRAFT_04633 - (3S)-malyl-CoA thioesterase, at 31,550 to 31,945
_04633
H281DRAFT_04634: H281DRAFT_04634 - ATP-binding cassette, subfamily B, at 32,255 to 34,144
_04634
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 29.729 kb on + strand at 29.729 kb on + strand at 29.730 kb on - strand at 29.730 kb on - strand at 29.730 kb on - strand at 29.735 kb on + strand at 30.041 kb on - strand, within H281DRAFT_04631 at 30.163 kb on + strand, within H281DRAFT_04631 at 30.163 kb on + strand, within H281DRAFT_04631 at 30.164 kb on - strand, within H281DRAFT_04631 at 30.164 kb on - strand, within H281DRAFT_04631 at 30.164 kb on - strand, within H281DRAFT_04631 at 30.260 kb on - strand, within H281DRAFT_04631 at 30.260 kb on - strand, within H281DRAFT_04631 at 30.263 kb on + strand, within H281DRAFT_04631 at 30.263 kb on + strand, within H281DRAFT_04631 at 30.320 kb on + strand at 30.320 kb on + strand at 30.320 kb on + strand at 30.320 kb on + strand at 30.320 kb on + strand at 30.320 kb on + strand at 30.320 kb on + strand at 30.320 kb on + strand at 30.321 kb on - strand at 30.321 kb on - strand at 30.321 kb on - strand at 30.321 kb on - strand at 30.321 kb on - strand at 30.337 kb on + strand at 30.337 kb on + strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.389 kb on + strand at 30.460 kb on + strand at 30.466 kb on - strand at 30.466 kb on - strand at 30.524 kb on + strand at 30.663 kb on + strand, within H281DRAFT_04632 at 30.663 kb on + strand, within H281DRAFT_04632 at 30.664 kb on - strand, within H281DRAFT_04632 at 30.664 kb on - strand, within H281DRAFT_04632 at 30.728 kb on + strand, within H281DRAFT_04632 at 30.729 kb on - strand, within H281DRAFT_04632 at 30.741 kb on + strand, within H281DRAFT_04632 at 30.741 kb on + strand, within H281DRAFT_04632 at 30.890 kb on + strand, within H281DRAFT_04632 at 30.891 kb on - strand, within H281DRAFT_04632 at 31.316 kb on + strand, within H281DRAFT_04632 at 31.329 kb on - strand, within H281DRAFT_04632 at 31.392 kb on + strand at 31.392 kb on + strand at 31.393 kb on - strand at 31.412 kb on + strand at 31.413 kb on - strand at 31.413 kb on - strand at 31.476 kb on + strand at 31.476 kb on + strand at 31.477 kb on - strand at 31.477 kb on - strand at 31.487 kb on + strand at 31.488 kb on - strand at 31.498 kb on + strand at 31.498 kb on + strand at 31.499 kb on - strand at 31.499 kb on - strand at 31.507 kb on + strand at 31.602 kb on + strand, within H281DRAFT_04633 at 31.602 kb on + strand, within H281DRAFT_04633 at 31.602 kb on + strand, within H281DRAFT_04633 at 31.602 kb on + strand, within H281DRAFT_04633 at 31.603 kb on - strand, within H281DRAFT_04633 at 31.622 kb on + strand, within H281DRAFT_04633 at 31.651 kb on + strand, within H281DRAFT_04633 at 31.651 kb on + strand, within H281DRAFT_04633 at 31.651 kb on + strand, within H281DRAFT_04633 at 31.662 kb on + strand, within H281DRAFT_04633 at 31.662 kb on + strand, within H281DRAFT_04633 at 31.663 kb on - strand, within H281DRAFT_04633 at 31.691 kb on - strand, within H281DRAFT_04633 at 31.829 kb on - strand, within H281DRAFT_04633 at 31.937 kb on - strand at 31.937 kb on - strand at 32.007 kb on - strand at 32.126 kb on + strand at 32.127 kb on - strand at 32.339 kb on + strand at 32.339 kb on + strand at 32.351 kb on + strand at 32.351 kb on + strand at 32.352 kb on - strand at 32.352 kb on - strand at 32.434 kb on + strand at 32.434 kb on + strand at 32.435 kb on - strand at 32.435 kb on - strand at 32.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 29,729 + +1.1 29,729 + -0.8 29,730 - +2.0 29,730 - +0.1 29,730 - +0.2 29,735 + -0.0 30,041 - H281DRAFT_04631 0.47 -1.1 30,163 + H281DRAFT_04631 0.67 +0.4 30,163 + H281DRAFT_04631 0.67 +0.6 30,164 - H281DRAFT_04631 0.67 +0.2 30,164 - H281DRAFT_04631 0.67 -0.2 30,164 - H281DRAFT_04631 0.67 -1.6 30,260 - H281DRAFT_04631 0.82 -0.1 30,260 - H281DRAFT_04631 0.82 +1.7 30,263 + H281DRAFT_04631 0.83 +0.2 30,263 + H281DRAFT_04631 0.83 -0.3 30,320 + +0.0 30,320 + +0.7 30,320 + +0.2 30,320 + +0.5 30,320 + -0.1 30,320 + +0.2 30,320 + -0.5 30,320 + +0.1 30,321 - +0.7 30,321 - +0.2 30,321 - +1.2 30,321 - +0.9 30,321 - +0.7 30,337 + -0.3 30,337 + -1.0 30,338 - +1.6 30,338 - +0.5 30,338 - +0.3 30,389 + +1.0 30,460 + -1.4 30,466 - +0.4 30,466 - -0.5 30,524 + +0.9 30,663 + H281DRAFT_04632 0.15 +0.2 30,663 + H281DRAFT_04632 0.15 -0.3 30,664 - H281DRAFT_04632 0.15 +0.4 30,664 - H281DRAFT_04632 0.15 -0.9 30,728 + H281DRAFT_04632 0.22 -0.9 30,729 - H281DRAFT_04632 0.22 -0.3 30,741 + H281DRAFT_04632 0.23 +0.1 30,741 + H281DRAFT_04632 0.23 +0.3 30,890 + H281DRAFT_04632 0.39 -1.3 30,891 - H281DRAFT_04632 0.40 +0.0 31,316 + H281DRAFT_04632 0.86 +0.6 31,329 - H281DRAFT_04632 0.87 -0.1 31,392 + -0.6 31,392 + +0.3 31,393 - +0.6 31,412 + +0.7 31,413 - +1.3 31,413 - +1.4 31,476 + -0.2 31,476 + +0.3 31,477 - -0.2 31,477 - -0.5 31,487 + +0.3 31,488 - -0.0 31,498 + +0.8 31,498 + +0.8 31,499 - -0.4 31,499 - +0.6 31,507 + -3.1 31,602 + H281DRAFT_04633 0.13 -1.4 31,602 + H281DRAFT_04633 0.13 +0.4 31,602 + H281DRAFT_04633 0.13 +0.0 31,602 + H281DRAFT_04633 0.13 +0.6 31,603 - H281DRAFT_04633 0.13 -0.3 31,622 + H281DRAFT_04633 0.18 +0.2 31,651 + H281DRAFT_04633 0.26 -0.8 31,651 + H281DRAFT_04633 0.26 -0.3 31,651 + H281DRAFT_04633 0.26 +0.3 31,662 + H281DRAFT_04633 0.28 +0.9 31,662 + H281DRAFT_04633 0.28 +0.3 31,663 - H281DRAFT_04633 0.29 +1.0 31,691 - H281DRAFT_04633 0.36 -1.0 31,829 - H281DRAFT_04633 0.70 +0.4 31,937 - +0.3 31,937 - +0.5 32,007 - +0.1 32,126 + -0.3 32,127 - +1.8 32,339 + -0.1 32,339 + +0.3 32,351 + +0.6 32,351 + +0.1 32,352 - +0.1 32,352 - +1.9 32,434 + -0.7 32,434 + +1.0 32,435 - -0.2 32,435 - +0.2 32,435 - +1.0
Or see this region's nucleotide sequence