Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04607 and H281DRAFT_04608 are separated by 37 nucleotides H281DRAFT_04608 and H281DRAFT_04609 are separated by 38 nucleotides H281DRAFT_04609 and H281DRAFT_04610 are separated by 494 nucleotides H281DRAFT_04610 and H281DRAFT_04611 are separated by 34 nucleotides H281DRAFT_04611 and H281DRAFT_04612 are separated by 78 nucleotides
H281DRAFT_04607: H281DRAFT_04607 - hypothetical protein, at 889 to 1,260
_04607
H281DRAFT_04608: H281DRAFT_04608 - hypothetical protein, at 1,298 to 1,474
_04608
H281DRAFT_04609: H281DRAFT_04609 - hypothetical protein, at 1,513 to 1,731
_04609
H281DRAFT_04610: H281DRAFT_04610 - Fe-S oxidoreductase, at 2,226 to 6,320
_04610
H281DRAFT_04611: H281DRAFT_04611 - Diadenosine tetraphosphate (Ap4A) hydrolase, at 6,355 to 6,783
_04611
H281DRAFT_04612: H281DRAFT_04612 - DUF971 family protein, at 6,862 to 7,275
_04612
Position (kb)
2
3
4
5
6
7 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 1.699 kb on - strand, within H281DRAFT_04609 at 1.844 kb on + strand at 2.078 kb on + strand at 2.078 kb on + strand at 2.079 kb on - strand at 2.311 kb on - strand at 2.316 kb on + strand at 2.316 kb on + strand at 2.317 kb on - strand at 2.317 kb on - strand at 2.356 kb on + strand at 2.431 kb on - strand at 2.431 kb on - strand at 2.473 kb on - strand at 2.473 kb on - strand at 2.685 kb on + strand, within H281DRAFT_04610 at 2.685 kb on + strand, within H281DRAFT_04610 at 2.685 kb on + strand, within H281DRAFT_04610 at 2.686 kb on - strand, within H281DRAFT_04610 at 2.686 kb on - strand, within H281DRAFT_04610 at 2.686 kb on - strand, within H281DRAFT_04610 at 3.356 kb on + strand, within H281DRAFT_04610 at 3.392 kb on + strand, within H281DRAFT_04610 at 3.515 kb on + strand, within H281DRAFT_04610 at 3.588 kb on + strand, within H281DRAFT_04610 at 3.588 kb on + strand, within H281DRAFT_04610 at 3.588 kb on + strand, within H281DRAFT_04610 at 3.589 kb on - strand, within H281DRAFT_04610 at 3.589 kb on - strand, within H281DRAFT_04610 at 3.589 kb on - strand, within H281DRAFT_04610 at 3.655 kb on - strand, within H281DRAFT_04610 at 3.959 kb on + strand, within H281DRAFT_04610 at 3.960 kb on - strand, within H281DRAFT_04610 at 4.191 kb on + strand, within H281DRAFT_04610 at 4.191 kb on + strand, within H281DRAFT_04610 at 4.191 kb on + strand, within H281DRAFT_04610 at 4.209 kb on + strand, within H281DRAFT_04610 at 4.210 kb on - strand, within H281DRAFT_04610 at 4.485 kb on + strand, within H281DRAFT_04610 at 4.485 kb on + strand, within H281DRAFT_04610 at 4.486 kb on - strand, within H281DRAFT_04610 at 4.486 kb on - strand, within H281DRAFT_04610 at 4.488 kb on - strand, within H281DRAFT_04610 at 4.583 kb on + strand, within H281DRAFT_04610 at 4.635 kb on - strand, within H281DRAFT_04610 at 4.635 kb on - strand, within H281DRAFT_04610 at 4.707 kb on + strand, within H281DRAFT_04610 at 4.707 kb on + strand, within H281DRAFT_04610 at 4.857 kb on + strand, within H281DRAFT_04610 at 4.912 kb on - strand, within H281DRAFT_04610 at 5.156 kb on + strand, within H281DRAFT_04610 at 5.156 kb on + strand, within H281DRAFT_04610 at 5.157 kb on - strand, within H281DRAFT_04610 at 5.422 kb on - strand, within H281DRAFT_04610 at 5.422 kb on - strand, within H281DRAFT_04610 at 5.422 kb on - strand, within H281DRAFT_04610 at 5.615 kb on - strand, within H281DRAFT_04610 at 5.615 kb on - strand, within H281DRAFT_04610 at 5.631 kb on + strand, within H281DRAFT_04610 at 5.631 kb on + strand, within H281DRAFT_04610 at 5.631 kb on + strand, within H281DRAFT_04610 at 5.632 kb on - strand, within H281DRAFT_04610 at 5.632 kb on - strand, within H281DRAFT_04610 at 5.673 kb on + strand, within H281DRAFT_04610 at 5.674 kb on - strand, within H281DRAFT_04610 at 5.790 kb on + strand, within H281DRAFT_04610 at 5.790 kb on + strand, within H281DRAFT_04610 at 5.790 kb on + strand, within H281DRAFT_04610 at 5.790 kb on + strand, within H281DRAFT_04610 at 5.791 kb on - strand, within H281DRAFT_04610 at 5.791 kb on - strand, within H281DRAFT_04610 at 5.791 kb on - strand, within H281DRAFT_04610 at 5.796 kb on + strand, within H281DRAFT_04610 at 5.797 kb on - strand, within H281DRAFT_04610 at 5.797 kb on - strand, within H281DRAFT_04610 at 5.797 kb on - strand, within H281DRAFT_04610 at 5.797 kb on - strand, within H281DRAFT_04610 at 5.797 kb on - strand, within H281DRAFT_04610 at 5.881 kb on - strand, within H281DRAFT_04610 at 6.190 kb on - strand at 6.190 kb on - strand at 6.219 kb on + strand at 6.219 kb on + strand at 6.220 kb on - strand at 6.220 kb on - strand at 6.220 kb on - strand at 6.273 kb on + strand at 6.273 kb on + strand at 6.274 kb on - strand at 6.318 kb on + strand at 6.443 kb on - strand, within H281DRAFT_04611 at 6.561 kb on + strand, within H281DRAFT_04611 at 6.561 kb on + strand, within H281DRAFT_04611 at 6.849 kb on + strand at 6.850 kb on - strand at 6.940 kb on + strand, within H281DRAFT_04612 at 6.940 kb on + strand, within H281DRAFT_04612 at 6.958 kb on + strand, within H281DRAFT_04612 at 7.090 kb on + strand, within H281DRAFT_04612 at 7.091 kb on - strand, within H281DRAFT_04612 at 7.135 kb on + strand, within H281DRAFT_04612 at 7.135 kb on + strand at 7.135 kb on + strand, within H281DRAFT_04612 at 7.135 kb on + strand, within H281DRAFT_04612 at 7.135 kb on + strand, within H281DRAFT_04612 at 7.136 kb on - strand, within H281DRAFT_04612 at 7.136 kb on - strand, within H281DRAFT_04612 at 7.153 kb on + strand, within H281DRAFT_04612 at 7.153 kb on + strand, within H281DRAFT_04612 at 7.192 kb on + strand, within H281DRAFT_04612 at 7.192 kb on + strand, within H281DRAFT_04612 at 7.276 kb on + strand at 7.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 1,699 - H281DRAFT_04609 0.85 -0.1 1,844 + -0.5 2,078 + +0.5 2,078 + -0.4 2,079 - -3.5 2,311 - -4.6 2,316 + -4.1 2,316 + -5.0 2,317 - -1.8 2,317 - -2.6 2,356 + -2.9 2,431 - -4.4 2,431 - -1.8 2,473 - -2.4 2,473 - -1.5 2,685 + H281DRAFT_04610 0.11 -5.4 2,685 + H281DRAFT_04610 0.11 -3.7 2,685 + H281DRAFT_04610 0.11 -3.0 2,686 - H281DRAFT_04610 0.11 -3.5 2,686 - H281DRAFT_04610 0.11 -3.2 2,686 - H281DRAFT_04610 0.11 -4.2 3,356 + H281DRAFT_04610 0.28 -3.2 3,392 + H281DRAFT_04610 0.28 -1.8 3,515 + H281DRAFT_04610 0.31 -5.3 3,588 + H281DRAFT_04610 0.33 -2.9 3,588 + H281DRAFT_04610 0.33 -4.5 3,588 + H281DRAFT_04610 0.33 -4.3 3,589 - H281DRAFT_04610 0.33 -4.3 3,589 - H281DRAFT_04610 0.33 -2.8 3,589 - H281DRAFT_04610 0.33 -0.8 3,655 - H281DRAFT_04610 0.35 -4.3 3,959 + H281DRAFT_04610 0.42 -6.6 3,960 - H281DRAFT_04610 0.42 -4.3 4,191 + H281DRAFT_04610 0.48 -4.1 4,191 + H281DRAFT_04610 0.48 -3.1 4,191 + H281DRAFT_04610 0.48 -4.1 4,209 + H281DRAFT_04610 0.48 -0.1 4,210 - H281DRAFT_04610 0.48 -3.6 4,485 + H281DRAFT_04610 0.55 -2.2 4,485 + H281DRAFT_04610 0.55 -3.5 4,486 - H281DRAFT_04610 0.55 +0.4 4,486 - H281DRAFT_04610 0.55 -4.9 4,488 - H281DRAFT_04610 0.55 -4.2 4,583 + H281DRAFT_04610 0.58 -3.3 4,635 - H281DRAFT_04610 0.59 -4.5 4,635 - H281DRAFT_04610 0.59 -2.7 4,707 + H281DRAFT_04610 0.61 -4.1 4,707 + H281DRAFT_04610 0.61 -2.1 4,857 + H281DRAFT_04610 0.64 -1.0 4,912 - H281DRAFT_04610 0.66 -3.7 5,156 + H281DRAFT_04610 0.72 -3.6 5,156 + H281DRAFT_04610 0.72 -5.4 5,157 - H281DRAFT_04610 0.72 -3.1 5,422 - H281DRAFT_04610 0.78 -2.3 5,422 - H281DRAFT_04610 0.78 -5.1 5,422 - H281DRAFT_04610 0.78 -1.3 5,615 - H281DRAFT_04610 0.83 -5.1 5,615 - H281DRAFT_04610 0.83 -2.1 5,631 + H281DRAFT_04610 0.83 -3.1 5,631 + H281DRAFT_04610 0.83 -0.7 5,631 + H281DRAFT_04610 0.83 -3.9 5,632 - H281DRAFT_04610 0.83 -3.5 5,632 - H281DRAFT_04610 0.83 -1.0 5,673 + H281DRAFT_04610 0.84 -4.1 5,674 - H281DRAFT_04610 0.84 -4.7 5,790 + H281DRAFT_04610 0.87 -4.2 5,790 + H281DRAFT_04610 0.87 -2.7 5,790 + H281DRAFT_04610 0.87 -4.3 5,790 + H281DRAFT_04610 0.87 -4.1 5,791 - H281DRAFT_04610 0.87 -5.2 5,791 - H281DRAFT_04610 0.87 -3.8 5,791 - H281DRAFT_04610 0.87 -3.4 5,796 + H281DRAFT_04610 0.87 -4.6 5,797 - H281DRAFT_04610 0.87 -2.9 5,797 - H281DRAFT_04610 0.87 -2.5 5,797 - H281DRAFT_04610 0.87 -4.4 5,797 - H281DRAFT_04610 0.87 -3.5 5,797 - H281DRAFT_04610 0.87 -4.5 5,881 - H281DRAFT_04610 0.89 -4.6 6,190 - -3.1 6,190 - -4.8 6,219 + -4.9 6,219 + -1.3 6,220 - -4.0 6,220 - -3.1 6,220 - -3.2 6,273 + -4.9 6,273 + -4.1 6,274 - -3.5 6,318 + +0.2 6,443 - H281DRAFT_04611 0.21 +0.8 6,561 + H281DRAFT_04611 0.48 +0.1 6,561 + H281DRAFT_04611 0.48 -0.3 6,849 + +0.1 6,850 - -1.3 6,940 + H281DRAFT_04612 0.19 -0.5 6,940 + H281DRAFT_04612 0.19 +0.1 6,958 + H281DRAFT_04612 0.23 +0.8 7,090 + H281DRAFT_04612 0.55 +0.3 7,091 - H281DRAFT_04612 0.55 +0.7 7,135 + H281DRAFT_04612 0.66 -0.2 7,135 + +1.0 7,135 + H281DRAFT_04612 0.66 -1.0 7,135 + H281DRAFT_04612 0.66 +0.7 7,135 + H281DRAFT_04612 0.66 -0.4 7,136 - H281DRAFT_04612 0.66 -0.2 7,136 - H281DRAFT_04612 0.66 -0.4 7,153 + H281DRAFT_04612 0.70 +0.1 7,153 + H281DRAFT_04612 0.70 -0.0 7,192 + H281DRAFT_04612 0.80 -0.7 7,192 + H281DRAFT_04612 0.80 -2.9 7,276 + -0.4 7,277 - +3.0
Or see this region's nucleotide sequence