Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04432 and H281DRAFT_04433 are separated by 650 nucleotides H281DRAFT_04433 and H281DRAFT_04434 are separated by 68 nucleotides H281DRAFT_04434 and H281DRAFT_04435 are separated by 73 nucleotides H281DRAFT_04435 and H281DRAFT_04436 are separated by 260 nucleotides
H281DRAFT_04432: H281DRAFT_04432 - Galactose mutarotase, at 149,014 to 149,922
_04432
H281DRAFT_04433: H281DRAFT_04433 - Undecaprenyl-diphosphatase, at 150,573 to 151,403
_04433
H281DRAFT_04434: H281DRAFT_04434 - tRNA_Arg_TCT, at 151,472 to 151,548
_04434
H281DRAFT_04435: H281DRAFT_04435 - Uncharacterized conserved protein, DUF427 family, at 151,622 to 152,005
_04435
H281DRAFT_04436: H281DRAFT_04436 - phospholipase C, at 152,266 to 153,843
_04436
Position (kb)
150
151
152 Strain fitness (log2 ratio)
-1
0
1
2
3 at 149.943 kb on - strand at 149.943 kb on - strand at 149.949 kb on + strand at 149.950 kb on - strand at 150.023 kb on + strand at 150.024 kb on - strand at 150.108 kb on + strand at 150.113 kb on - strand at 150.143 kb on - strand at 150.203 kb on + strand at 150.204 kb on - strand at 150.212 kb on + strand at 150.418 kb on + strand at 150.418 kb on + strand at 150.419 kb on - strand at 150.419 kb on - strand at 150.419 kb on - strand at 150.419 kb on - strand at 150.419 kb on - strand at 150.419 kb on - strand at 150.419 kb on - strand at 150.441 kb on + strand at 150.492 kb on + strand at 150.492 kb on + strand at 150.493 kb on - strand at 150.559 kb on - strand at 150.591 kb on - strand at 150.650 kb on + strand at 150.650 kb on + strand at 150.651 kb on - strand at 150.651 kb on - strand at 150.683 kb on + strand, within H281DRAFT_04433 at 150.683 kb on + strand, within H281DRAFT_04433 at 150.787 kb on + strand, within H281DRAFT_04433 at 150.905 kb on - strand, within H281DRAFT_04433 at 151.302 kb on + strand, within H281DRAFT_04433 at 151.405 kb on - strand at 151.600 kb on + strand at 151.600 kb on + strand at 151.624 kb on - strand at 151.685 kb on + strand, within H281DRAFT_04435 at 151.705 kb on - strand, within H281DRAFT_04435 at 151.716 kb on + strand, within H281DRAFT_04435 at 151.716 kb on + strand, within H281DRAFT_04435 at 151.764 kb on + strand, within H281DRAFT_04435 at 151.767 kb on + strand, within H281DRAFT_04435 at 151.767 kb on + strand, within H281DRAFT_04435 at 151.767 kb on + strand, within H281DRAFT_04435 at 151.767 kb on + strand, within H281DRAFT_04435 at 151.851 kb on + strand, within H281DRAFT_04435 at 151.852 kb on - strand, within H281DRAFT_04435 at 151.855 kb on - strand, within H281DRAFT_04435 at 151.867 kb on - strand, within H281DRAFT_04435 at 152.073 kb on - strand at 152.125 kb on + strand at 152.143 kb on - strand at 152.143 kb on - strand at 152.170 kb on + strand at 152.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 149,943 - +2.5 149,943 - -0.3 149,949 + -0.2 149,950 - +0.2 150,023 + -0.9 150,024 - -0.9 150,108 + +0.5 150,113 - +0.5 150,143 - +0.6 150,203 + +0.8 150,204 - +1.1 150,212 + +0.7 150,418 + +0.5 150,418 + -1.0 150,419 - -0.6 150,419 - +0.6 150,419 - +0.6 150,419 - +0.2 150,419 - +0.2 150,419 - -0.7 150,419 - -0.0 150,441 + -1.1 150,492 + +1.5 150,492 + +0.1 150,493 - -0.0 150,559 - +0.2 150,591 - +1.0 150,650 + -0.3 150,650 + +0.4 150,651 - +0.4 150,651 - -0.1 150,683 + H281DRAFT_04433 0.13 +0.8 150,683 + H281DRAFT_04433 0.13 -0.8 150,787 + H281DRAFT_04433 0.26 -1.2 150,905 - H281DRAFT_04433 0.40 +0.9 151,302 + H281DRAFT_04433 0.88 +0.7 151,405 - +0.0 151,600 + +0.2 151,600 + +1.6 151,624 - +0.2 151,685 + H281DRAFT_04435 0.16 +0.3 151,705 - H281DRAFT_04435 0.22 +0.6 151,716 + H281DRAFT_04435 0.24 -0.4 151,716 + H281DRAFT_04435 0.24 +1.2 151,764 + H281DRAFT_04435 0.37 +0.9 151,767 + H281DRAFT_04435 0.38 -0.8 151,767 + H281DRAFT_04435 0.38 +0.7 151,767 + H281DRAFT_04435 0.38 +0.1 151,767 + H281DRAFT_04435 0.38 -0.8 151,851 + H281DRAFT_04435 0.60 -0.2 151,852 - H281DRAFT_04435 0.60 -0.7 151,855 - H281DRAFT_04435 0.61 +3.1 151,867 - H281DRAFT_04435 0.64 -0.1 152,073 - +0.3 152,125 + -0.7 152,143 - -0.1 152,143 - +0.3 152,170 + -0.1 152,242 - +0.1
Or see this region's nucleotide sequence