Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04398 and H281DRAFT_04399 are separated by 19 nucleotides H281DRAFT_04399 and H281DRAFT_04400 are separated by 156 nucleotides H281DRAFT_04400 and H281DRAFT_04401 are separated by 201 nucleotides
H281DRAFT_04398: H281DRAFT_04398 - Methylase involved in ubiquinone/menaquinone biosynthesis, at 112,775 to 113,680
_04398
H281DRAFT_04399: H281DRAFT_04399 - hydroxyacylglutathione hydrolase, at 113,700 to 114,491
_04399
H281DRAFT_04400: H281DRAFT_04400 - membrane-bound lytic murein transglycosylase D, at 114,648 to 116,297
_04400
H281DRAFT_04401: H281DRAFT_04401 - Cyanate permease, at 116,499 to 117,737
_04401
Position (kb)
114
115
116
117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 113.660 kb on + strand at 113.661 kb on - strand at 113.661 kb on - strand at 113.681 kb on - strand at 113.716 kb on - strand at 113.720 kb on - strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.746 kb on - strand at 113.746 kb on - strand at 113.746 kb on - strand at 113.818 kb on - strand, within H281DRAFT_04399 at 113.854 kb on + strand, within H281DRAFT_04399 at 113.926 kb on - strand, within H281DRAFT_04399 at 114.020 kb on - strand, within H281DRAFT_04399 at 114.057 kb on + strand, within H281DRAFT_04399 at 114.226 kb on - strand, within H281DRAFT_04399 at 114.433 kb on + strand at 114.434 kb on - strand at 114.434 kb on - strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.456 kb on - strand at 114.586 kb on + strand at 114.656 kb on + strand at 114.656 kb on + strand at 114.656 kb on + strand at 114.657 kb on - strand at 114.979 kb on - strand, within H281DRAFT_04400 at 114.984 kb on + strand, within H281DRAFT_04400 at 115.016 kb on + strand at 115.059 kb on + strand, within H281DRAFT_04400 at 115.061 kb on + strand, within H281DRAFT_04400 at 115.131 kb on + strand, within H281DRAFT_04400 at 115.132 kb on - strand, within H281DRAFT_04400 at 115.132 kb on - strand, within H281DRAFT_04400 at 115.149 kb on - strand, within H281DRAFT_04400 at 115.203 kb on + strand, within H281DRAFT_04400 at 115.204 kb on - strand, within H281DRAFT_04400 at 115.204 kb on - strand, within H281DRAFT_04400 at 115.326 kb on + strand, within H281DRAFT_04400 at 115.326 kb on + strand, within H281DRAFT_04400 at 115.327 kb on - strand, within H281DRAFT_04400 at 115.327 kb on - strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.363 kb on - strand, within H281DRAFT_04400 at 115.413 kb on + strand, within H281DRAFT_04400 at 115.450 kb on - strand, within H281DRAFT_04400 at 115.509 kb on - strand, within H281DRAFT_04400 at 115.575 kb on - strand, within H281DRAFT_04400 at 115.668 kb on + strand, within H281DRAFT_04400 at 115.668 kb on + strand, within H281DRAFT_04400 at 115.668 kb on + strand, within H281DRAFT_04400 at 115.669 kb on - strand, within H281DRAFT_04400 at 115.669 kb on - strand, within H281DRAFT_04400 at 116.295 kb on + strand at 116.295 kb on + strand at 116.296 kb on - strand at 116.296 kb on - strand at 116.373 kb on - strand at 116.498 kb on + strand at 116.498 kb on + strand at 116.499 kb on - strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.594 kb on + strand at 116.690 kb on - strand, within H281DRAFT_04401 at 116.690 kb on - strand, within H281DRAFT_04401 at 116.692 kb on + strand, within H281DRAFT_04401 at 116.692 kb on + strand, within H281DRAFT_04401 at 116.692 kb on + strand, within H281DRAFT_04401 at 116.693 kb on - strand, within H281DRAFT_04401 at 117.107 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.171 kb on + strand, within H281DRAFT_04401 at 117.171 kb on + strand, within H281DRAFT_04401
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 113,660 + -1.0 113,661 - -1.5 113,661 - -1.7 113,681 - +0.5 113,716 - +0.3 113,720 - -0.4 113,745 + -0.9 113,745 + -0.6 113,745 + -0.4 113,745 + -1.3 113,745 + -3.3 113,745 + +0.1 113,746 - +0.1 113,746 - -0.6 113,746 - +0.6 113,818 - H281DRAFT_04399 0.15 +0.1 113,854 + H281DRAFT_04399 0.19 +0.3 113,926 - H281DRAFT_04399 0.29 -0.4 114,020 - H281DRAFT_04399 0.40 -0.2 114,057 + H281DRAFT_04399 0.45 -3.1 114,226 - H281DRAFT_04399 0.66 +0.9 114,433 + -0.7 114,434 - +0.9 114,434 - +1.3 114,455 + -1.1 114,455 + -0.3 114,455 + +2.1 114,455 + -0.6 114,455 + +0.9 114,456 - +1.5 114,586 + +1.0 114,656 + +1.5 114,656 + +1.7 114,656 + +0.7 114,657 - +0.1 114,979 - H281DRAFT_04400 0.20 +2.2 114,984 + H281DRAFT_04400 0.20 +3.2 115,016 + +1.4 115,059 + H281DRAFT_04400 0.25 +1.3 115,061 + H281DRAFT_04400 0.25 +0.7 115,131 + H281DRAFT_04400 0.29 +0.7 115,132 - H281DRAFT_04400 0.29 -0.5 115,132 - H281DRAFT_04400 0.29 +1.3 115,149 - H281DRAFT_04400 0.30 +1.1 115,203 + H281DRAFT_04400 0.34 +1.4 115,204 - H281DRAFT_04400 0.34 +0.6 115,204 - H281DRAFT_04400 0.34 +1.2 115,326 + H281DRAFT_04400 0.41 +1.4 115,326 + H281DRAFT_04400 0.41 +0.5 115,327 - H281DRAFT_04400 0.41 +0.9 115,327 - H281DRAFT_04400 0.41 +0.0 115,330 + H281DRAFT_04400 0.41 +1.5 115,330 + H281DRAFT_04400 0.41 +1.1 115,330 + H281DRAFT_04400 0.41 -0.0 115,330 + H281DRAFT_04400 0.41 +1.6 115,330 + H281DRAFT_04400 0.41 +0.0 115,331 - H281DRAFT_04400 0.41 +1.3 115,331 - H281DRAFT_04400 0.41 +2.7 115,331 - H281DRAFT_04400 0.41 +0.0 115,331 - H281DRAFT_04400 0.41 +1.0 115,331 - H281DRAFT_04400 0.41 +1.4 115,331 - H281DRAFT_04400 0.41 +2.4 115,331 - H281DRAFT_04400 0.41 +1.4 115,362 + H281DRAFT_04400 0.43 +0.4 115,362 + H281DRAFT_04400 0.43 +1.1 115,362 + H281DRAFT_04400 0.43 +0.8 115,362 + H281DRAFT_04400 0.43 +0.9 115,363 - H281DRAFT_04400 0.43 +1.5 115,413 + H281DRAFT_04400 0.46 +0.1 115,450 - H281DRAFT_04400 0.49 +2.6 115,509 - H281DRAFT_04400 0.52 +1.2 115,575 - H281DRAFT_04400 0.56 +1.0 115,668 + H281DRAFT_04400 0.62 -0.2 115,668 + H281DRAFT_04400 0.62 +0.6 115,668 + H281DRAFT_04400 0.62 +1.0 115,669 - H281DRAFT_04400 0.62 +1.5 115,669 - H281DRAFT_04400 0.62 +1.6 116,295 + +1.4 116,295 + +1.1 116,296 - -0.8 116,296 - -1.5 116,373 - +0.7 116,498 + +1.9 116,498 + +0.1 116,499 - -0.3 116,556 + -0.9 116,556 + +1.1 116,556 + +0.3 116,556 + -0.1 116,556 + -0.7 116,556 + -0.2 116,557 - -0.0 116,557 - +0.1 116,557 - +0.3 116,557 - +0.1 116,557 - +0.3 116,557 - -0.3 116,557 - +0.5 116,594 + +0.2 116,690 - H281DRAFT_04401 0.15 +0.5 116,690 - H281DRAFT_04401 0.15 +0.3 116,692 + H281DRAFT_04401 0.16 +1.5 116,692 + H281DRAFT_04401 0.16 +0.2 116,692 + H281DRAFT_04401 0.16 -0.8 116,693 - H281DRAFT_04401 0.16 -0.4 117,107 + H281DRAFT_04401 0.49 -0.5 117,112 + H281DRAFT_04401 0.49 +0.8 117,112 + H281DRAFT_04401 0.49 +0.3 117,112 + H281DRAFT_04401 0.49 +0.8 117,112 + H281DRAFT_04401 0.49 +0.6 117,171 + H281DRAFT_04401 0.54 +0.0 117,171 + H281DRAFT_04401 0.54 -0.6
Or see this region's nucleotide sequence