Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04317 and H281DRAFT_04318 are separated by 323 nucleotides H281DRAFT_04318 and H281DRAFT_04319 are separated by 311 nucleotides H281DRAFT_04319 and H281DRAFT_04320 are separated by 81 nucleotides H281DRAFT_04320 and H281DRAFT_04321 are separated by 218 nucleotides
H281DRAFT_04317: H281DRAFT_04317 - multidrug resistance protein, MATE family, at 35,283 to 36,695
_04317
H281DRAFT_04318: H281DRAFT_04318 - ribosome-associated protein, at 37,019 to 37,237
_04318
H281DRAFT_04319: H281DRAFT_04319 - hypothetical protein, at 37,549 to 38,013
_04319
H281DRAFT_04320: H281DRAFT_04320 - hypothetical protein, at 38,095 to 38,376
_04320
H281DRAFT_04321: H281DRAFT_04321 - DNA helicase-2 / ATP-dependent DNA helicase PcrA, at 38,595 to 40,724
_04321
Position (kb) 37
38
39 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 36.787 kb on - strand at 36.834 kb on + strand at 36.835 kb on - strand at 36.835 kb on - strand at 36.928 kb on + strand at 36.928 kb on + strand at 36.929 kb on - strand at 37.058 kb on + strand, within H281DRAFT_04318 at 37.058 kb on + strand, within H281DRAFT_04318 at 37.059 kb on - strand, within H281DRAFT_04318 at 37.203 kb on + strand, within H281DRAFT_04318 at 37.272 kb on + strand at 37.273 kb on - strand at 37.273 kb on - strand at 37.451 kb on + strand at 37.452 kb on - strand at 37.533 kb on + strand at 37.533 kb on + strand at 37.533 kb on + strand at 37.534 kb on - strand at 37.534 kb on - strand at 37.563 kb on + strand at 37.563 kb on + strand at 37.563 kb on + strand at 37.564 kb on - strand at 37.564 kb on - strand at 37.564 kb on - strand at 37.656 kb on + strand, within H281DRAFT_04319 at 37.657 kb on - strand, within H281DRAFT_04319 at 37.664 kb on + strand, within H281DRAFT_04319 at 37.664 kb on + strand, within H281DRAFT_04319 at 37.664 kb on + strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.782 kb on - strand, within H281DRAFT_04319 at 37.782 kb on - strand, within H281DRAFT_04319 at 37.885 kb on + strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 38.004 kb on + strand at 38.004 kb on + strand at 38.081 kb on + strand at 38.112 kb on + strand at 38.374 kb on + strand at 38.375 kb on - strand at 38.375 kb on - strand at 38.375 kb on - strand at 38.432 kb on + strand at 38.466 kb on + strand at 38.467 kb on - strand at 38.510 kb on + strand at 38.547 kb on + strand at 38.547 kb on + strand at 38.548 kb on - strand at 38.586 kb on + strand at 38.586 kb on + strand at 38.774 kb on - strand at 38.866 kb on + strand, within H281DRAFT_04321 at 38.867 kb on - strand, within H281DRAFT_04321 at 38.867 kb on - strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.871 kb on - strand, within H281DRAFT_04321 at 38.871 kb on - strand, within H281DRAFT_04321 at 38.871 kb on - strand, within H281DRAFT_04321 at 38.939 kb on + strand, within H281DRAFT_04321 at 38.967 kb on + strand, within H281DRAFT_04321 at 38.968 kb on - strand, within H281DRAFT_04321
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 36,787 - +0.6 36,834 + -0.2 36,835 - -1.0 36,835 - -1.4 36,928 + +0.6 36,928 + +0.5 36,929 - -0.6 37,058 + H281DRAFT_04318 0.18 -0.1 37,058 + H281DRAFT_04318 0.18 +0.2 37,059 - H281DRAFT_04318 0.18 +0.2 37,203 + H281DRAFT_04318 0.84 +1.1 37,272 + -0.5 37,273 - +3.5 37,273 - -0.3 37,451 + +1.3 37,452 - -0.3 37,533 + +0.3 37,533 + -0.5 37,533 + -0.5 37,534 - +0.2 37,534 - -0.6 37,563 + +0.3 37,563 + +3.3 37,563 + +0.6 37,564 - -0.2 37,564 - -0.0 37,564 - -0.4 37,656 + H281DRAFT_04319 0.23 +0.2 37,657 - H281DRAFT_04319 0.23 -0.1 37,664 + H281DRAFT_04319 0.25 +0.1 37,664 + H281DRAFT_04319 0.25 -0.2 37,664 + H281DRAFT_04319 0.25 -0.4 37,665 - H281DRAFT_04319 0.25 +0.2 37,665 - H281DRAFT_04319 0.25 -0.1 37,665 - H281DRAFT_04319 0.25 -0.4 37,665 - H281DRAFT_04319 0.25 +0.4 37,782 - H281DRAFT_04319 0.50 +0.3 37,782 - H281DRAFT_04319 0.50 -1.3 37,885 + H281DRAFT_04319 0.72 -0.5 37,886 - H281DRAFT_04319 0.72 +0.2 37,886 - H281DRAFT_04319 0.72 +0.3 37,886 - H281DRAFT_04319 0.72 -0.2 37,886 - H281DRAFT_04319 0.72 -0.2 38,004 + +1.1 38,004 + +0.8 38,081 + +0.1 38,112 + +0.6 38,374 + +0.5 38,375 - -1.1 38,375 - -0.9 38,375 - -0.4 38,432 + -0.6 38,466 + +0.6 38,467 - +0.0 38,510 + +0.8 38,547 + +0.1 38,547 + +0.0 38,548 - +1.2 38,586 + -0.6 38,586 + +3.8 38,774 - +0.4 38,866 + H281DRAFT_04321 0.13 -0.2 38,867 - H281DRAFT_04321 0.13 -0.2 38,867 - H281DRAFT_04321 0.13 -1.6 38,870 + H281DRAFT_04321 0.13 -0.4 38,870 + H281DRAFT_04321 0.13 -0.3 38,870 + H281DRAFT_04321 0.13 -0.0 38,870 + H281DRAFT_04321 0.13 -0.6 38,870 + H281DRAFT_04321 0.13 -0.7 38,870 + H281DRAFT_04321 0.13 -0.6 38,870 + H281DRAFT_04321 0.13 +1.5 38,871 - H281DRAFT_04321 0.13 -0.2 38,871 - H281DRAFT_04321 0.13 +0.2 38,871 - H281DRAFT_04321 0.13 -0.3 38,939 + H281DRAFT_04321 0.16 -0.1 38,967 + H281DRAFT_04321 0.17 +1.8 38,968 - H281DRAFT_04321 0.18 -0.2
Or see this region's nucleotide sequence