Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04314 and H281DRAFT_04315 are separated by 15 nucleotides H281DRAFT_04315 and H281DRAFT_04316 are separated by 157 nucleotides
H281DRAFT_04314: H281DRAFT_04314 - fumarase, class II, at 30,617 to 32,017
_04314
H281DRAFT_04315: H281DRAFT_04315 - Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthase, at 32,033 to 33,682
_04315
H281DRAFT_04316: H281DRAFT_04316 - bile acid:Na+ symporter, BASS family, at 33,840 to 34,799
_04316
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 31.214 kb on + strand, within H281DRAFT_04314 at 31.215 kb on - strand, within H281DRAFT_04314 at 31.215 kb on - strand, within H281DRAFT_04314 at 31.347 kb on + strand, within H281DRAFT_04314 at 31.348 kb on - strand, within H281DRAFT_04314 at 31.348 kb on - strand, within H281DRAFT_04314 at 31.348 kb on - strand, within H281DRAFT_04314 at 31.405 kb on - strand, within H281DRAFT_04314 at 31.988 kb on - strand at 31.988 kb on - strand at 32.034 kb on + strand at 32.034 kb on + strand at 32.035 kb on - strand at 32.066 kb on + strand at 32.066 kb on + strand at 32.067 kb on - strand at 32.067 kb on - strand at 32.088 kb on + strand at 32.088 kb on + strand at 32.089 kb on - strand at 32.091 kb on - strand at 32.116 kb on - strand at 32.116 kb on - strand at 32.223 kb on + strand, within H281DRAFT_04315 at 32.379 kb on + strand at 32.380 kb on - strand, within H281DRAFT_04315 at 32.380 kb on - strand, within H281DRAFT_04315 at 32.460 kb on + strand, within H281DRAFT_04315 at 32.462 kb on + strand, within H281DRAFT_04315 at 32.505 kb on + strand, within H281DRAFT_04315 at 32.625 kb on + strand, within H281DRAFT_04315 at 32.625 kb on + strand, within H281DRAFT_04315 at 32.626 kb on - strand, within H281DRAFT_04315 at 32.806 kb on + strand, within H281DRAFT_04315 at 32.807 kb on - strand, within H281DRAFT_04315 at 33.042 kb on + strand, within H281DRAFT_04315 at 33.151 kb on + strand, within H281DRAFT_04315 at 33.151 kb on + strand, within H281DRAFT_04315 at 33.151 kb on + strand, within H281DRAFT_04315 at 33.152 kb on - strand, within H281DRAFT_04315 at 33.310 kb on - strand, within H281DRAFT_04315 at 33.360 kb on + strand, within H281DRAFT_04315 at 33.360 kb on + strand, within H281DRAFT_04315 at 33.360 kb on + strand, within H281DRAFT_04315 at 33.360 kb on + strand, within H281DRAFT_04315 at 33.361 kb on - strand, within H281DRAFT_04315 at 33.363 kb on + strand, within H281DRAFT_04315 at 33.363 kb on + strand, within H281DRAFT_04315 at 33.364 kb on - strand, within H281DRAFT_04315 at 33.364 kb on - strand, within H281DRAFT_04315 at 33.640 kb on + strand at 33.641 kb on - strand at 33.759 kb on + strand at 33.759 kb on + strand at 33.760 kb on - strand at 33.880 kb on + strand at 33.881 kb on - strand at 33.991 kb on + strand, within H281DRAFT_04316 at 33.992 kb on - strand, within H281DRAFT_04316 at 33.992 kb on - strand, within H281DRAFT_04316 at 34.077 kb on + strand, within H281DRAFT_04316 at 34.078 kb on - strand, within H281DRAFT_04316 at 34.078 kb on - strand, within H281DRAFT_04316 at 34.078 kb on - strand, within H281DRAFT_04316 at 34.370 kb on - strand, within H281DRAFT_04316 at 34.379 kb on + strand, within H281DRAFT_04316 at 34.380 kb on - strand, within H281DRAFT_04316 at 34.380 kb on - strand, within H281DRAFT_04316 at 34.462 kb on + strand, within H281DRAFT_04316 at 34.463 kb on - strand, within H281DRAFT_04316 at 34.463 kb on - strand, within H281DRAFT_04316 at 34.585 kb on + strand, within H281DRAFT_04316 at 34.586 kb on - strand, within H281DRAFT_04316
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 31,214 + H281DRAFT_04314 0.43 +0.7 31,215 - H281DRAFT_04314 0.43 -0.1 31,215 - H281DRAFT_04314 0.43 -1.0 31,347 + H281DRAFT_04314 0.52 -0.3 31,348 - H281DRAFT_04314 0.52 -0.4 31,348 - H281DRAFT_04314 0.52 +0.4 31,348 - H281DRAFT_04314 0.52 -0.5 31,405 - H281DRAFT_04314 0.56 -0.8 31,988 - -1.6 31,988 - -0.3 32,034 + +0.1 32,034 + +1.1 32,035 - +0.1 32,066 + -0.2 32,066 + +1.4 32,067 - -0.3 32,067 - +0.3 32,088 + +0.1 32,088 + -1.2 32,089 - -0.8 32,091 - +0.9 32,116 - +0.1 32,116 - +0.1 32,223 + H281DRAFT_04315 0.12 -2.0 32,379 + +1.4 32,380 - H281DRAFT_04315 0.21 -0.4 32,380 - H281DRAFT_04315 0.21 -0.5 32,460 + H281DRAFT_04315 0.26 -0.6 32,462 + H281DRAFT_04315 0.26 +0.4 32,505 + H281DRAFT_04315 0.29 +1.8 32,625 + H281DRAFT_04315 0.36 -0.5 32,625 + H281DRAFT_04315 0.36 -0.7 32,626 - H281DRAFT_04315 0.36 +0.1 32,806 + H281DRAFT_04315 0.47 +0.4 32,807 - H281DRAFT_04315 0.47 +0.1 33,042 + H281DRAFT_04315 0.61 -0.8 33,151 + H281DRAFT_04315 0.68 +0.2 33,151 + H281DRAFT_04315 0.68 -0.5 33,151 + H281DRAFT_04315 0.68 +0.2 33,152 - H281DRAFT_04315 0.68 +0.5 33,310 - H281DRAFT_04315 0.77 -0.6 33,360 + H281DRAFT_04315 0.80 +0.1 33,360 + H281DRAFT_04315 0.80 -0.2 33,360 + H281DRAFT_04315 0.80 +0.0 33,360 + H281DRAFT_04315 0.80 +0.2 33,361 - H281DRAFT_04315 0.80 +0.6 33,363 + H281DRAFT_04315 0.81 -0.9 33,363 + H281DRAFT_04315 0.81 -0.1 33,364 - H281DRAFT_04315 0.81 +0.1 33,364 - H281DRAFT_04315 0.81 +0.1 33,640 + +0.7 33,641 - +0.0 33,759 + +0.5 33,759 + -0.5 33,760 - +1.5 33,880 + +0.2 33,881 - +0.5 33,991 + H281DRAFT_04316 0.16 +2.5 33,992 - H281DRAFT_04316 0.16 +0.1 33,992 - H281DRAFT_04316 0.16 +0.3 34,077 + H281DRAFT_04316 0.25 -0.1 34,078 - H281DRAFT_04316 0.25 +1.2 34,078 - H281DRAFT_04316 0.25 -0.7 34,078 - H281DRAFT_04316 0.25 -0.3 34,370 - H281DRAFT_04316 0.55 -0.1 34,379 + H281DRAFT_04316 0.56 +1.3 34,380 - H281DRAFT_04316 0.56 +0.0 34,380 - H281DRAFT_04316 0.56 -2.8 34,462 + H281DRAFT_04316 0.65 +0.2 34,463 - H281DRAFT_04316 0.65 +0.0 34,463 - H281DRAFT_04316 0.65 +0.6 34,585 + H281DRAFT_04316 0.78 +0.7 34,586 - H281DRAFT_04316 0.78 +0.7
Or see this region's nucleotide sequence