Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04249 and H281DRAFT_04250 overlap by 4 nucleotides H281DRAFT_04250 and H281DRAFT_04251 are separated by 56 nucleotides H281DRAFT_04251 and H281DRAFT_04252 are separated by 120 nucleotides H281DRAFT_04252 and H281DRAFT_04253 are separated by 128 nucleotides
H281DRAFT_04249: H281DRAFT_04249 - tRNA-U20-dihydrouridine synthase, at 369,070 to 370,146
_04249
H281DRAFT_04250: H281DRAFT_04250 - DNA-binding protein Fis, at 370,143 to 370,376
_04250
H281DRAFT_04251: H281DRAFT_04251 - IMP cyclohydrolase /phosphoribosylaminoimidazolecarboxamide formyltransferase, at 370,433 to 371,998
_04251
H281DRAFT_04252: H281DRAFT_04252 - hypothetical protein, at 372,119 to 372,358
_04252
H281DRAFT_04253: H281DRAFT_04253 - Holliday junction endonuclease RuvC, at 372,487 to 373,029
_04253
Position (kb) 370
371
372 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 369.617 kb on - strand, within H281DRAFT_04249 at 369.634 kb on + strand, within H281DRAFT_04249 at 369.696 kb on - strand, within H281DRAFT_04249 at 369.696 kb on - strand, within H281DRAFT_04249 at 369.887 kb on - strand, within H281DRAFT_04249 at 369.895 kb on + strand, within H281DRAFT_04249 at 369.896 kb on - strand, within H281DRAFT_04249 at 369.943 kb on + strand, within H281DRAFT_04249 at 369.944 kb on - strand, within H281DRAFT_04249 at 369.990 kb on + strand, within H281DRAFT_04249 at 369.991 kb on - strand, within H281DRAFT_04249 at 370.072 kb on + strand at 370.136 kb on + strand at 370.154 kb on + strand at 370.154 kb on + strand at 370.157 kb on + strand at 370.157 kb on + strand at 370.158 kb on - strand at 370.191 kb on + strand, within H281DRAFT_04250 at 370.191 kb on + strand, within H281DRAFT_04250 at 370.230 kb on + strand, within H281DRAFT_04250 at 370.230 kb on + strand, within H281DRAFT_04250 at 370.318 kb on - strand at 370.337 kb on + strand, within H281DRAFT_04250 at 370.337 kb on + strand, within H281DRAFT_04250 at 370.337 kb on + strand, within H281DRAFT_04250 at 370.374 kb on + strand at 370.374 kb on + strand at 370.374 kb on + strand at 370.384 kb on + strand at 372.039 kb on + strand at 372.049 kb on + strand at 372.050 kb on - strand at 372.137 kb on - strand at 372.177 kb on + strand, within H281DRAFT_04252 at 372.177 kb on + strand, within H281DRAFT_04252 at 372.177 kb on + strand, within H281DRAFT_04252 at 372.177 kb on + strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.255 kb on + strand, within H281DRAFT_04252 at 372.256 kb on - strand, within H281DRAFT_04252 at 372.261 kb on + strand at 372.261 kb on + strand, within H281DRAFT_04252 at 372.261 kb on + strand, within H281DRAFT_04252 at 372.261 kb on + strand, within H281DRAFT_04252 at 372.262 kb on - strand at 372.262 kb on - strand, within H281DRAFT_04252 at 372.262 kb on - strand, within H281DRAFT_04252 at 372.374 kb on + strand at 372.374 kb on + strand at 372.374 kb on + strand at 372.375 kb on - strand at 372.375 kb on - strand at 372.375 kb on - strand at 372.375 kb on - strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.445 kb on - strand at 372.445 kb on - strand at 372.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 369,617 - H281DRAFT_04249 0.51 -0.2 369,634 + H281DRAFT_04249 0.52 -1.8 369,696 - H281DRAFT_04249 0.58 -0.9 369,696 - H281DRAFT_04249 0.58 +0.6 369,887 - H281DRAFT_04249 0.76 +0.1 369,895 + H281DRAFT_04249 0.77 +0.7 369,896 - H281DRAFT_04249 0.77 -1.2 369,943 + H281DRAFT_04249 0.81 -1.4 369,944 - H281DRAFT_04249 0.81 -0.0 369,990 + H281DRAFT_04249 0.85 -0.7 369,991 - H281DRAFT_04249 0.86 +3.1 370,072 + +2.1 370,136 + -0.4 370,154 + +1.0 370,154 + +1.9 370,157 + +3.0 370,157 + +1.5 370,158 - +1.0 370,191 + H281DRAFT_04250 0.21 +1.0 370,191 + H281DRAFT_04250 0.21 +1.8 370,230 + H281DRAFT_04250 0.37 -0.2 370,230 + H281DRAFT_04250 0.37 +0.1 370,318 - -0.0 370,337 + H281DRAFT_04250 0.83 +1.2 370,337 + H281DRAFT_04250 0.83 +1.2 370,337 + H281DRAFT_04250 0.83 +1.2 370,374 + +0.7 370,374 + -0.3 370,374 + +0.9 370,384 + +0.5 372,039 + +1.8 372,049 + -1.7 372,050 - -1.1 372,137 - -0.4 372,177 + H281DRAFT_04252 0.24 -1.7 372,177 + H281DRAFT_04252 0.24 +0.2 372,177 + H281DRAFT_04252 0.24 +0.0 372,177 + H281DRAFT_04252 0.24 -0.4 372,178 - H281DRAFT_04252 0.25 -0.3 372,178 - H281DRAFT_04252 0.25 -0.3 372,178 - H281DRAFT_04252 0.25 +0.8 372,178 - H281DRAFT_04252 0.25 -1.2 372,255 + H281DRAFT_04252 0.57 +0.4 372,256 - H281DRAFT_04252 0.57 -0.9 372,261 + +3.3 372,261 + H281DRAFT_04252 0.59 -1.0 372,261 + H281DRAFT_04252 0.59 -0.3 372,261 + H281DRAFT_04252 0.59 +0.6 372,262 - +2.0 372,262 - H281DRAFT_04252 0.60 -0.6 372,262 - H281DRAFT_04252 0.60 -1.4 372,374 + -0.3 372,374 + +0.2 372,374 + +0.9 372,375 - +0.2 372,375 - -0.0 372,375 - -0.2 372,375 - -0.5 372,444 + +3.0 372,444 + -1.7 372,444 + -1.6 372,444 + +0.3 372,444 + +1.0 372,444 + +0.8 372,445 - -1.3 372,445 - -0.2 372,447 + +0.4
Or see this region's nucleotide sequence